24 results on '"Omayma A. Eldahshan"'
Search Results
2. Essential Oil of Cestrum diurnum L.: GC/MS Analysis, in Vitro and in Silico Anti‐HCoV‐229E Effects and Inhibitory Activity against LPS‐Induced Inflammation
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Dina M. Bahgat, Haidy A. Gad, Eman Al‐Sayed, Sara H. Mahmoud, Ahmed Mostafa, Nadia M. Mahfouz, Omayma A. Eldahshan, and Abdel Nasser B. Singab
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Molecular Medicine ,Bioengineering ,General Chemistry ,General Medicine ,Molecular Biology ,Biochemistry - Published
- 2023
3. Insights on benzodiazepines' potential in Alzheimer's disease
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Hayder M. Al-Kuraishy, Ali I. Al-Gareeb, Abdulrahman A. Alsayegh, Wafaa Fouzi Abusudah, Najlaa Hamed Almohmadi, Omayma A. Eldahshan, Eman A. Ahmed, and Gaber El-Saber Batiha
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General Medicine ,General Pharmacology, Toxicology and Pharmaceutics ,General Biochemistry, Genetics and Molecular Biology - Published
- 2023
4. Orange fruit (Citrus sinensis) peel extract attenuates chemotherapy-induced toxicity in male rats
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Eman A. Abdelghffar, Ameera G A Al-Mohammadi, Heba A. S. El-Nashar, and Omayma A. Eldahshan
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Antioxidant ,Limonin ,medicine.medical_treatment ,Diosmin ,General Medicine ,Pharmacology ,medicine.disease_cause ,Lipid peroxidation ,chemistry.chemical_compound ,Hesperidin ,chemistry ,Toxicity ,medicine ,Naringin ,Oxidative stress ,Food Science ,medicine.drug - Abstract
Background: Cyclophosphamide (CYP) is a chemotherapy drug widely used in the treatment of several types of cancers and autoimmune disorders. Unfortunately, it causes severe side effects on many organs due to its oxidative stress effect. Objective: The present study aims to tentatively identify the phytochemical constituents of orange fruit (Citrus sinensis) peel extract (OFPE) and elucidate the chemopreventive effects of OFPE on CYP drug induced organ toxicity. Methods: The high performance liquid chromatography coupled with mass spectroscopy (HPLC-MS/MS) technique was used to identify the compounds. Thirty-five male rats were divided into five groups (GP; n = 7): GP1: normal control, GP2: OFPE 0.5 only, GP3: CYP-only, GP4: OFPE 0.25 + CYP, and GP5: OFPE 0.5 + CYP. Results: Twenty-nine compounds of polyphenolic nature, mainly flavonoids, anthocyanidins, phenolic acids and limonoids were characterized by HPLC-MS/MS analysis. Among these compounds, naringin, hesperidin, diosmin, rutin, neohesperidin and limonin were the predominant compounds in the examined extract. Serum cellular markers were found to be decreased significantly upon treatment with OFPE (especially high dose). Also, a significant prophylactic effect against liver, kidney, and heart injuries induced by CYP via decreasing inflammation (serum TNF-α, IL-1β & IL-6) and lipid peroxidation (MDA) was also revealed. Also, an increase in antioxidant levels (serum TAO, and cellular GSH & CAT in tissue homogenates) confirmed the protective efficacy of OFPE against CYP toxicity. Conclusions: The present study reveals some chemopreventive properties and beneficial effects of OFPE on CYP-induced organ toxicity via its antioxidant status and immunoregulatory activities.
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- 2021
5. UPLC-ESI/MS/MS Profiling and Anti-Inflammatory Activity of Gleditsia caspica
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Amany Seleem, Ashaimaa Y Moussa, Hager Ashraf, Abdel Nasser B. Singab, and Omayma A. Eldahshan
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chemistry.chemical_classification ,biology ,Traditional medicine ,Chemistry ,medicine.drug_class ,Electrospray ionization ,Phytosterol ,lcsh:RM1-950 ,Glycoside ,Uplc esi ms ms ,glycosides ,General Medicine ,biology.organism_classification ,fabaceae ,Anti-inflammatory ,lcsh:Therapeutics. Pharmacology ,inflammation ,Gleditsia caspica ,flavonoids ,medicine ,gleditsia caspica ,Gleditsia ,Paw edema - Abstract
The Caspian Locust is the common name of Gleditsia caspica, a tree cultivated in the southern and western Caspian Sea in Russia and Iran. Herein, a qualitative characterization of the chemical constituents of Gleditsia caspica leaves extract using ultra-performance liquid chromatography coupled to electrospray ionization tandem mass fragmentation (UPLC-ESI/MS/MS) analysis was performed revealing the presence of nine compounds: six flavonoids, one triterpenoid, one phytosterol, and one long chain ester. Six compounds were newly reported from Gleditsia caspica and two for the first time from genus Gleditsia. Moreover, the in-vivo anti-inflammatory activities of two different doses; 50 and 100 mg/kg of the aqueous-alcoholic extract of Gleditsia caspica leaves were evaluated in the carrageenan-induced paw edema model. The percentage of edema inhibition was calculated after 1, 2, 3, and 4 h of the administration showing a significant reduction in edema thickness in a dose and time-dependent manner compared to the control drug, indomethacin. These results put forward Gleditsia caspica extract as a potential natural anti-inflammatory agent with minimal side effects.
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- 2020
6. New γ-pyrone glycoside from Pachira glabra and assessment of its gastroprotective activity using an alcohol-induced gastric ulcer model in rats
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Mohamed L. Ashour, Mariam I. Gamal El-Din, Omayma A. Eldahshan, Fadia S. Youssef, and Abdel Nasser B. Singab
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Male ,0301 basic medicine ,Pachira glabra ,Alcohol ,01 natural sciences ,03 medical and health sciences ,chemistry.chemical_compound ,In vivo ,Bombacaceae ,medicine ,Animals ,Glycosides ,Stomach Ulcer ,Rats, Wistar ,Omeprazole ,chemistry.chemical_classification ,Ethanol ,Traditional medicine ,Plant Extracts ,Glycoside ,General Medicine ,Anti-Ulcer Agents ,Pyrone ,Rats ,0104 chemical sciences ,Disease Models, Animal ,010404 medicinal & biomolecular chemistry ,030104 developmental biology ,Phytochemical ,chemistry ,Gastric Mucosa ,Food Science ,medicine.drug - Abstract
Pachira glabra is a medium sized tree that is famous for its delicious edible seeds and leaves. Phytochemical investigation of Pachira glabra leaf alcohol extract led to the isolation of a new γ-pyrone glycoside, 7-hydroxy maltol-3-O-β-d-glucoside (HMGlu). In vivo assessment of the gastro-protective activity of HMGlu demonstrated superior gastro-protection at a dose of 100 mg kg-1, close to the value triggered by the standard reference omeprazole drug (evidenced by morphological and histopathological examinations). This was further confirmed by immunohistochemical staining where pretreatment with HMGlu (100 mg kg-1) markedly reduced NFκB, COX-2 and BAX positively-stained cells. Thus, it can be concluded that 7-hydroxy maltol-3-O-β-d-glucoside can be a new pharmaceutically active agent with promising gastro-protective activity of natural origin.
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- 2020
7. Ajwa dates (Phoenix dactylifera L.) attenuate cisplatin-induced nephrotoxicity in rats via augmenting Nrf2, modulating NADPH oxidase-4 and mitigating inflammatory/apoptotic mediators
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Eman A. Abdelghffar, Wael A. Obaid, Zuhair M. Mohammedsaleh, Wafae Ouchari, Omayma A. Eldahshan, and Mansour Sobeh
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Male ,Pharmacology ,NF-E2-Related Factor 2 ,Plant Extracts ,Phoeniceae ,Hydrogen Peroxide ,General Medicine ,GA-Binding Protein Transcription Factor ,Antioxidants ,Rats ,Oxidative Stress ,Phenols ,Tandem Mass Spectrometry ,Animals ,RNA, Messenger ,Cisplatin ,Inflammation Mediators ,Chromatography, Liquid - Abstract
In the therapy of cisplatin (CP), nephrotoxicity is a main limiting issue that associated with oxidative stress and apoptosis. According to many studies, the antioxidant and anti-inflammatory properties of Ajwa dates are very strong, due to the unique phytochemical profile. Here, we investigated the possible mitigative effects of Ajwa dates fruits extract (ADFE) vs CP-induced nephrotoxicity in rats, in addition to phytochemical profiling of its components via LC-MS/MS. Six groups were formed from forty-two male rats. G1: control, G2: ADFE 0.25 g/kg, G3: ADFE 0.5 g/kg (for 21 days), G4: CP -intoxicated group (single i.p. dose of 7.0 mg/kg b.w) on day 16th, G5: ADFE 0.25 + CP, G6: ADFE 0.5 + CP. LC-MS/MS analysis revealed the tentative identification of 17 compounds of different chemical nature, including organic/phenolic acids, and flavonoids and their sulphated/glycosides derivatives. ADFE has considerable antioxidant potential (DPPH with IC
- Published
- 2022
8. Chemical profiling, antiviral and antiproliferative activities of the essential oil of Phlomis aurea Decne grown in Egypt
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Eman A. Abdelghffar, Zenab Aly Torky, Usama K. Abdel-Hameed, Ashaimaa Y Moussa, and Omayma A. Eldahshan
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0301 basic medicine ,01 natural sciences ,Antiviral Agents ,Gas Chromatography-Mass Spectrometry ,Flow cytometry ,law.invention ,03 medical and health sciences ,Phlomis ,Sesquiterpenes, Germacrane ,law ,Cell Line, Tumor ,medicine ,Oils, Volatile ,Humans ,Plant Oils ,MTT assay ,Cytotoxicity ,Essential oil ,Bicyclic Monoterpenes ,Binding Sites ,medicine.diagnostic_test ,biology ,Chemistry ,Plant Extracts ,Cell Cycle ,Cyclin-Dependent Kinase 2 ,General Medicine ,Hep G2 Cells ,Cell cycle ,biology.organism_classification ,0104 chemical sciences ,Molecular Docking Simulation ,010404 medicinal & biomolecular chemistry ,030104 developmental biology ,Biochemistry ,Apoptosis ,Cell culture ,Egypt ,Sesquiterpenes ,Food Science - Abstract
Here, we investigated the chemical composition of the edible Phlomis aurea oil and its anticancer potential on three human cancer cell lines, as well as its antiviral activity against Herpes simplex-1 (HSV-1). Exploring Phlomis aurea Decne essential oil by gas chromatography coupled with mass spectrometry (GC/MS) revealed the presence of four major components: germacrene D (51.56%), trans-β-farnesene (11.36%), α-pinene (22.96%) & limonene (6.26%). An antiproliferative effect, as determined by the MTT assay, against human hepatic, breast and colon cancer cell lines, manifested IC50 values of 10.14, 328.02, & 628.43 μg mL-1, respectively. Cytotoxicity assay of the Phlomis oil against Vero cell lines revealed a safe profile within the range of 50 μg ml-1. Phlomis essential oil induced the apoptosis of HepG2 cells through increasing cell accumulation in sub G1 & G2/M phases, decreasing both S & G0/G1 phases of the cell cycle, triggering both caspases-3 &-9, and inhibiting cyclin dependent kinase-2 (CDK2). The antiviral activity of the oil against HSV-1 was investigated using the plaque reduction assay, which showed 80% of virus inhibition. Moreover, the molecular docking in silico study of the four major chemical constituents of the oil at the CDK2 binding site demonstrated marked interactions with the ATP-binding site residues through alkyl & Pi-alkyl interactions. Cell cycle distribution of HepG2 cells was studied using flow cytometry to highlight the apoptotic mechanistic approaches by measuring caspases-3 &-9 and CDK2 activities. Thus, the edible Phlomis oil can be regarded as a candidate for in vivo studies to prove that it is a promising natural antiviral/anticancer agent.
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- 2021
9. Metabolic profiling of a polyphenolic-rich fraction of Coccinia grandis leaves using LC-ESI-MS/MS and in vivo validation of its antimicrobial and wound healing activities
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Nada M Mostafa, Somaia. A. Al-Madhagy, Abdel Nasser B. Singab, Ghada E. A. Awad, Omayma A. Eldahshan, and Fadia S. Youssef
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Male ,0301 basic medicine ,Coccinia grandis ,food.ingredient ,Scars ,Bacterial growth ,03 medical and health sciences ,chemistry.chemical_compound ,Rutin ,food ,Bacillus cereus ,Tandem Mass Spectrometry ,Oleuropein ,In vivo ,medicine ,Animals ,Humans ,Chromatography, High Pressure Liquid ,Gram-Positive Bacterial Infections ,Wound Healing ,030109 nutrition & dietetics ,Chromatography ,Plant Extracts ,Polyphenols ,General Medicine ,Antimicrobial ,Anti-Bacterial Agents ,Rats ,Plant Leaves ,Cucurbitaceae ,030104 developmental biology ,chemistry ,Wounds and Injuries ,medicine.symptom ,Wound healing ,Food Science - Abstract
A polyphenolic-rich fraction (CG50) was obtained from the methanol extract of Coccinia grandis leaves by chromatographic fractionation over a Diaion column using 50% aqueous methanol. LC-ESI-MS/MS analysis of CG50 showed the presence of six flavonoids, namely quercetin-hexoside deoxyhexoside (rutin), quercetin-hexoside deoxyhexoside (quercetin-3-O-neohesperidoside), kaempferol-hexoside deoxyhexoside (kaempferol-3-O-rutinoside), kaempferol-hexoside deoxyhexoside (kaempferol-3-O-neohesperidoside), kaempferol-3-O-glucoside, and kaempferol-hexoside in addition to the presence of two secoiridoids which are oleuropein and ligstroside. CG50 hydrogel showed a pronounced inhibition of the bacterial growth in wounds infected by Bacillus cereus in rats comparable to those treated with hydrogel base only showing 85.08 and 16.50% inhibition for the bacterial growth for the CG50 hydrogel and hydrogel base, respectively. The antimicrobial activity of CG50 hydrogel was close to that of fucidin during all days of treatment. Rats treated with CG50 hydrogel showed remarkable healing ability of the wound compared to other groups and approaching that of fucidin. This was clearly manifested by the clear formation of scars with obvious reduction in the wound size together with the appearance and re-growth of hair. This was further confirmed by the histopathological study of skin tissues as well as by the evaluation of the percentages of collagen fiber deposition. CG50 hydrogel showed 18.71% collagen fiber deposition comparable to the untreated group that showed 6.84% collagen fiber deposition and approaches that of the fucidin group. It was concluded that Coccinia grandis could be used as a natural wound healing agent that further consolidated its traditional use as a wound dressing.
- Published
- 2019
10. The protective role ofOcimum basilicumL. (Basil) against aspirin-induced gastric ulcer in mice: Impact on oxidative stress, inflammation, motor deficits and anxiety-like behavior
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Eman A. Abd El-Ghffar, Thomas Efferth, Safia Mohamed Shehata, Omayma A. Eldahshan, and Eman Al-Sayed
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Male ,food.ingredient ,medicine.drug_class ,Anxiety ,Motor Activity ,Pharmacology ,Protective Agents ,Ulcer index ,medicine.disease_cause ,030226 pharmacology & pharmacy ,Anxiolytic ,Mice ,03 medical and health sciences ,0302 clinical medicine ,food ,TBARS ,Animals ,Humans ,Medicine ,Stomach Ulcer ,Misoprostol ,Aspirin ,Behavior, Animal ,biology ,Interleukin-6 ,Plant Extracts ,Tumor Necrosis Factor-alpha ,business.industry ,Basilicum ,General Medicine ,Ocimum ,biology.organism_classification ,Plant Leaves ,Oxidative Stress ,Ocimum basilicum ,Female ,Interleukin-4 ,business ,030217 neurology & neurosurgery ,Oxidative stress ,Food Science ,medicine.drug - Abstract
The gastroprotective effect of Ocimum basilicum L. (Basil) hexane extract (OBHE) in aspirin-induced gastric ulcers in mice and its ameliorative effect on behavioral alterations were determined. Pretreatment with OBHE (100 or 200 mg kg-1) or misoprostol (50 μg kg-1) alleviated the aspirin-induced oxidative stress by significantly decreasing (p < 0.001) gastric ulcer index scores (57, 76 and 79%), gastric TBARS (by 49, 51 and 52%), NO (21, 28 and 29%), H2O2 (24, 42 and 45%), and the serum pro-inflammatory mediator TNF-α (21, 53 and 53%) and IL-6 (29, 30 and 31%), as well as by markedly increasing gastric GSH (41, 61 and 70%), GSH-Px (21, 32 and 34%), GST (33, 63 and 70%), GR (90, 99 and 112%), CAT (167, 211 and 267%) and serum PGE-2 levels (22, 135 and 200%) and IL-4 (64, 81 and 104%), respectively, compared with the aspirin-treated group. Meanwhile, OBHE and misoprostol induced a significant decrease (p < 0.001) in the freezing time (53, 56 and 64%), and the grooming time (by 25, 43 and 44%), respectively, compared to the aspirin treated group. This study provides evidence that OBHE confers anxiolytic, antioxidant and anti-inflammatory prophylactic effects on aspirin-induced gastric ulcers. GC/MS was used for the characterization of OBHE components. Based on the findings of this study, basil may be used as a nutritional supplement or therapeutic drug to protect against aspirin-induced gastric ulcers, a common problem resulting from the use of aspirin.
- Published
- 2018
11. The prophylactic effect of aEugenia aqueaextract against oxidative stress and inflammation associated with the development of arthritis in an adjuvant-induced arthritis rat model
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Alaa Barakat, Thomas Efferth, Eman A. Abd El-Ghffar, and Omayma A. Eldahshan
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0301 basic medicine ,Antioxidant ,medicine.medical_treatment ,Anti-Inflammatory Agents ,Arthritis ,Inflammation ,Pharmacology ,medicine.disease_cause ,Eugenia ,Antioxidants ,Borneol ,Arthritis, Rheumatoid ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Diclofenac ,medicine ,Animals ,Humans ,Bicyclic Monoterpenes ,Eucalyptol ,Plant Extracts ,business.industry ,General Medicine ,medicine.disease ,Rats ,Oxidative Stress ,030104 developmental biology ,chemistry ,030220 oncology & carcinogenesis ,Rheumatoid arthritis ,Monoterpenes ,Female ,medicine.symptom ,business ,Oxidative stress ,Food Science ,medicine.drug - Abstract
Rheumatoid arthritis (RA) is the most common human autoimmune disease. A petroleum ether extract of Eugenia aquea (E. aquea) was analyzed by GC/MS. Antioxidant and anti-inflammatory activities were investigated in rats with adjuvant-induced arthritis (AIA). An AIA rat model received orally/daily a vehicle, diclofenac (100 mg per kg b.w.), and E. aquea extract (50 or 100 or 200 mg per kg b.w.; for 21 days). Fifty-five out of 70 compounds (97.77%) were identified: eucalyptol (34.14%), α-pinene (15.91%), l-verbenone (8.01%), camphor (7.38%) and borneol (6.74%). In an acute oral toxicity study, the E. aquea extract did not show any toxic effects in rats at 2000 mg/ kg-1. Only a high dose of the E. aquea extract or diclofenac significantly alleviated (P < 0.05-0.001) all complications observed in arthritic rats, including body weight loss, articular/extra-articular oxidative injury and synovial joint inflammation by increasing food intake as well as improving the antioxidant defense system and inflammatory marker. The dose-dependent modulatory activity of the E. aquea extract was statistically significant. It was equivalent to and sometimes even better than that of diclofenac. The present study proved the antioxidant and anti-inflammatory activities of the E. aquea extract, which could be attributed to the presence of eucalyptol and α-pinene.
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- 2018
12. HPLC Standardization of The Methanolic Extract of Acrocarpus fraxinifolius leaves based on Gallic acid Content
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Omayma A. Eldahshan, Heba A. S. El-Nashar, and Abdel Nasser B. Singab
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Detection limit ,linearity ,Chromatography ,biology ,Correlation coefficient ,Gallic acid ,lcsh:RM1-950 ,General Medicine ,biology.organism_classification ,High-performance liquid chromatography ,Standardization ,quantification ,chemistry.chemical_compound ,lcsh:Therapeutics. Pharmacology ,chemistry ,HPLC ,Acrocarpus fraxinifolius ,Reference standards ,Accuracy - Abstract
High-Performance Liquid Chromatography (HPLC) was developed for the standardization and quantification of gallic acid in the methanolic extract of Acrocarpus fraxinifolius Weight & Arn leaves. Linearity was observed in the range of 0.4–2 mg/mL with a correlation coefficient (r2) of 0.9978. The limit of detection (LOD) and limit of quantification (LOQ) were 0.0054 mg/mL and 0.0163 mg/mL, respectively indicating the sensitivity of the applied method. Recovery values of 100.377 % indicate the best accuracy of the method. Gallic acid content was quantified as 168.75 ± 1.05 mg/g methanolic extract. This developed method was simple, accurate and precise to be used as a reference standard method for determination of gallic acid content in the plant extract.
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- 2017
13. Genus Enterolobium : traditional uses, chemistry and biological activities
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Omayma A. Eldahshan, Abdel Nasser B. Singab, Nahla Ayoub, and Mariam I. Gamal El-Din
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Antifungal ,Traditional uses ,Traditional medicine ,biology ,medicine.drug_class ,Chemistry ,lcsh:RM1-950 ,biological activities ,chemical constituents ,Biological activity ,General Medicine ,Fabaceae ,biology.organism_classification ,Enterolobium ,Terpene ,Enteroobium ,lcsh:Therapeutics. Pharmacology ,Genus ,medicine - Abstract
The chemical composition, pharmacological activity and traditional uses of 20 species attributed to the genus Enterolobium (Fabaceae) as used in the South and Central America, and Tropical Africa, were revised and compared. A survey of the available literature shows that these species are used mostly for their anti-inflammatory and cytotoxic activities. Additionally, some of these Enterolobium species showed antibacterial, antifungal, insecticidal, molluscicidal and larvicidal activities. Generally, the triterpenes or the phenolic compounds isolated from these plant extracts are assumed to be the bioactive principles.
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- 2017
14. Anti-inflammatory and gastroprotective potential of leaf essential oil of Cinnamomum glanduliferum in ethanol-induced rat experimental gastritis
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Gehad A. Abdel Jaleel, Samar S. Azab, and Omayma A. Eldahshan
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Male ,Cinnamomum glanduliferum ,Anti-Inflammatory Agents ,Pharmaceutical Science ,medicine.disease_cause ,01 natural sciences ,law.invention ,Lipid peroxidation ,chemistry.chemical_compound ,law ,Drug Discovery ,oxidative stress ,Traditional medicine ,biology ,Chemistry ,lipid peroxidation ,General Medicine ,Lauraceae ,pge2 ,Gastritis ,visual_art ,visual_art.visual_art_medium ,Molecular Medicine ,Egypt ,Bark ,Research Article ,medicine.drug_class ,Context (language use) ,gc-ms ,Gas Chromatography-Mass Spectrometry ,Anti-inflammatory ,Gastrointestinal Agents ,Botany ,Oils, Volatile ,medicine ,Animals ,Rats, Wistar ,Essential oil ,Cinnamomum ,Pharmacology ,Dose-Response Relationship, Drug ,010405 organic chemistry ,lcsh:RM1-950 ,biology.organism_classification ,Rats ,0104 chemical sciences ,Plant Leaves ,Disease Models, Animal ,010404 medicinal & biomolecular chemistry ,lcsh:Therapeutics. Pharmacology ,Complementary and alternative medicine ,Cyclooxygenase 2 ,inflammation ,Oxidative stress - Abstract
Context: Nothing could be found in the literature concerning Cinnamomum glanduliferum (Wall) Meissn (Lauraceae) bark (CG) in Egypt. Objective: To investigate CG volatile oil chemically and its anti-inflammatory and gastroprotective effects. Materials and methods: Essential oils were investigated by GC-MS. Leaves oil was assessed at doses of 250, 500 and 1000 mg/kg for its anti-inflammatory effect against carrageenan-induced rat oedema model. Serum inflammation markers were measured. The gastro-protective effect of the same doses of the volatile oil was also tested in ethanol-induced non-ulcerative gastritis model in rats. Stomach oxidative stress markers were examined following 1 h after intragastric ethanol administration. Results: Twenty-five and 20 compounds were identified from leaf and branch oils, respectively (98.85 and 99.13%). The major ones were: eucalyptol (59.44%; 55.74%), sabinene (14.99%; 7.12%), α-terpineol (6.44%; 9.81%), α-pinene (5.27%; 4.71%). Following 4 h of treatment leaves volatile oil at doses of 250, 500 and 1000 mg/kg significantly reduced paw volume to 94, 82 and 69%, respectively. The same doses significantly reduced COX-2 activity to 73.8, 50.7 and 21.4 nmol/min/mL, respectively. A significant reduction of PGE2 concentration was observed (2.95 ± 0.2, 2.45 ± 0.15 and 1.75 ± 0.015 pg/mL). CG oil exhibited a significant modulatory effect on ethanol-induced gastritis in rats as the level of NO reduced to 32, 37 and 41 μM nitrate/g and also a significant inhibition of lipid peroxidation was observed via reduction of MDA concentration (1.15, 1.11 and 1.04 nmol/g). Conclusion: CG volatile oil exhibited an anti-inflammatory effect and protected against ethanol-induced non-ulcerative gastritis.
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- 2017
15. GC-MS analysis and hepatoprotective activity of then-hexane extract ofAcrocarpus fraxinifoliusleaves against paracetamol-induced hepatotoxicity in male albino rats
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Heba A. S. El-Nashar, Eman A. Abd El-Ghffar, Omayma A. Eldahshan, and Abdel Nasser B. Singab
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Male ,0301 basic medicine ,gas chromatography mass spectrometry ,Pharmaceutical Science ,Antioxidants ,chemistry.chemical_compound ,Squalene ,0302 clinical medicine ,Drug Discovery ,acetaminophen ,biology ,Traditional medicine ,liver dysfunction ,digestive, oral, and skin physiology ,Fabaceae ,Organ Size ,General Medicine ,Lipids ,Hexane ,Liver ,030220 oncology & carcinogenesis ,Molecular Medicine ,Chemical and Drug Induced Liver Injury ,medicine.drug ,Gas Chromatography-Mass Spectrometry ,Lethal Dose 50 ,03 medical and health sciences ,Phytol ,Weight Loss ,Healthy control ,medicine ,Animals ,Hexanes ,Rats, Wistar ,Lupeol ,Pharmacology ,Plants, Medicinal ,Chromatography ,Dose-Response Relationship, Drug ,Plant Extracts ,lcsh:RM1-950 ,Bilirubin ,biology.organism_classification ,ink cedar ,Acetaminophen ,Plant Leaves ,Disease Models, Animal ,Oxidative Stress ,lcsh:Therapeutics. Pharmacology ,030104 developmental biology ,Complementary and alternative medicine ,chemistry ,Solvents ,Gas chromatography–mass spectrometry ,Acrocarpus fraxinifolius ,Biomarkers ,Phytotherapy - Abstract
Context: In Egypt, the burden of liver diseases is exceptionally high. Objective: To investigate the components of the n-hexane extract of Acrocarpus fraxinifolius Arn. (Leguminosae) and its hepatoprotective activity against paracetamol (APAP)-induced hepatotoxicity in rats. Material and methods: TRACE GC ultra gas chromatogaphic spectrometry was used for extract analysis. Thirty albino rats were divided into six groups (five rats in each). Group 1 was the healthy control; Groups 2 and 3 were healthy treated groups (250 and 500 mg/kg b.w. of the extract, respectively) for seven days. Group 4 was hepatotoxicity control (APAP intoxicated group). Groups 5 and 6 received APAP + extract 250 and APAP + extract 500, respectively. Results: Chromatographic analysis revealed the presence of 36 components. Major compounds were α-tocopherol (18.23%), labda-8 (20)-13-dien-15-oic acid (13.15%), lupeol (11.93%), phytol (10.95%) and squalene (7.19%). In the acute oral toxicity study, the mortality rates and behavioural signs of toxicity were zero in all groups (doses from 0 to 5 g/kg b.w. of A. fraxinifolius). LD50 was found to be greater than 5 g/kg of the extract. Only the high dose (500 mg/kg b.w.) of extract significantly alleviated the liver relative weight (4.01 ± 0.06) and biomarkers, as serum aspartate aminotransferase (62.87 ± 1.41), alanine aminotransferase (46.74 ± 1.45), alkaline phosphatase (65.96 ± 0.74), lipid profiles (180.39 ± 3.51), bilirubin profiles (2.30 ± 0.06) and hepatic lipid peroxidation (114.20 ± 2.06), and increased body weight (11.58 ± 0.20), serum protein profile (11.09 ± 0.46) and hepatic total antioxidant capacity (23.78 ± 0.66) in APAP-induced hepatotoxicity in rats. Conclusion: Our study proves the antihepatotoxic/antioxidant efficacies of A. fraxinifolius hexane extract.
- Published
- 2016
16. Cytotoxic Oleanane-Type Saponins from the Leaves of Albizia anthelmintica <scp>Brongn.</scp>
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Omayma A. Eldahshan, Dina M. Bahgat, Eman Al-Sayed, and Abdel Nasser B. Singab
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Stereochemistry ,Molecular Conformation ,Albizzia ,Bioengineering ,01 natural sciences ,Biochemistry ,Inhibitory Concentration 50 ,Structure-Activity Relationship ,chemistry.chemical_compound ,Humans ,Cytotoxic T cell ,MTT assay ,Oleanolic Acid ,Cytotoxicity ,Molecular Biology ,Oleanane ,IC50 ,Cell Proliferation ,Aqueous solution ,Dose-Response Relationship, Drug ,010405 organic chemistry ,Hep G2 Cells ,General Chemistry ,General Medicine ,Saponins ,HCT116 Cells ,Antineoplastic Agents, Phytogenic ,0104 chemical sciences ,Plant Leaves ,010404 medicinal & biomolecular chemistry ,chemistry ,Molecular Medicine ,Acid hydrolysis ,Drug Screening Assays, Antitumor ,Heteronuclear single quantum coherence spectroscopy - Abstract
Two new oleanane-type saponins: β-d-xylopyranosyl-(1 → 4)-6-deoxy-α-l-mannopyranosyl-(1 → 2)-1-O-{(3β)-28-oxo-3-[(2-O-β-d-xylopyranosyl-β-d-glucopyranosyl)oxy]olean-12-en-28-yl}-β-d-glucopyranose (1) and 1-O-[(3β)-28-oxo-3-{[β-d-xylopyranosyl-(1 → 2)-α-l-arabinopyranosyl-(1 → 6)-2-acetamido-2-deoxy-β-d-glucopyranosyl]oxy}olean-12-en-28-yl]β-d-glucopyranose (2), along with two known saponins: (3β)-3-[(β-d-Glucopyranosyl-(1 → 2)-β-d-glucopyranosyl)oxy]olean-12-en-28-oic acid (3) and (3β)-3-{[α-l-arabinopyranosyl-(1 → 6)-[β-d-glucopyranosyl-(1 → 2)]-β-d-glucopyranosyl]oxy}olean-12-en-28-oic acid (4) were isolated from the acetone-insoluble fraction obtained from the 80% aqueous MeOH extract of Albizia anthelmintica Brongn. leaves. Their structures were identified using different NMR experiments including: 1 H- and 13 C-NMR, HSQC, HMBC and 1 H,1 H-COSY, together with HR-ESI-MS/MS, as well as by acid hydrolysis. The four isolated saponins and the fractions of the extract exhibited cytotoxic activity against HepG-2 and HCT-116 cell lines. Compound 2 showed the most potent cytotoxic activity among the other tested compounds against the HepG2 cell line with an IC50 value of 3.60μm. Whereas, compound 1 showed the most potent cytotoxic effect with an IC50 value of 4.75μm on HCT-116 cells.
- Published
- 2016
17. Modulatory effect of Prosopis juliflora leaves on hepatic fibrogenic and fibrolytic alterations induced in rats by thioacetamide
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Ahmed A. Sayed, Salah M. Hassan, AlShaimaa M. Taha, Omayma A. Eldahshan, and Ahmed M. Salem
- Subjects
0301 basic medicine ,Liver Cirrhosis ,Necrosis ,Extracellular matrix removal ,RM1-950 ,Pharmacology ,Thioacetamide ,03 medical and health sciences ,Hydroxyproline ,chemistry.chemical_compound ,Mice ,0302 clinical medicine ,Alkaloids ,Prosopis ,Fibrosis ,medicine ,Animals ,Regeneration ,Rats, Wistar ,IC50 ,Plant Extracts ,Polyphenols ,General Medicine ,medicine.disease ,Flavones ,Rats ,Plant Leaves ,030104 developmental biology ,chemistry ,Phytochemical ,Polyphenol ,030220 oncology & carcinogenesis ,Therapeutics. Pharmacology ,medicine.symptom ,Hepatic fibrosis ,Phytotherapy - Abstract
This study investigated the antifibrotic effect of Prosopis juliflora leaves crude methanolic extract (PJEL) against thioacetamide (TAA)-induced liver fibrosis. The phytochemical analysis of PJEL was performed via HPLC/MS in association with evaluating its free radical scavenging and cytotoxic activities. The antifibrotic activity of PJEL was assessed by dividing Wistar rats into 8 groups: normal control, PJEL1-administered rats (2 mg/ Kg b.w.), PJEL2-administered rats (4 mg/ Kg b.w.), PJEL3-administered rats (8 mg/Kg b.w.), TAA-induced hepatic fibrosis, TTA + PJEL1, TAA + PJEL2, and TAA + PJEL3. Results indicated that PJEL crude methanolic extract is rich in polyphenolic compounds and alkaloids. PJEL exerted free radical scavenging activity with IC50 of 123.5 μg/mL and cytotoxic activity against a well-differentiated hepatocellular cell line (IC50 = 11.1 μg/mL). PJEL at a dose of 4 mg/Kg b.w. ameliorated serum ALT activity and improved serum albumin level and hepatic hydroxyproline content in association with a reduction in the fibrosis stage. PJEL elevated hepatic tumor necrosis factor-α and interleukin-6 contents with less necrosis grade. PJEL post-therapy ameliorated the relative expression of Bcl-2, Col1A1, Mmp-9, and Mmp-2 genes in liver. Conclusion PJEL possesses a good therapeutic activity against TAA-induced liver fibrosis via enhancing extracellular matrix removal and stimulating hepatic regeneration to decrease hepatic necrosis.
- Published
- 2019
18. Hepatoprotective Natural Products
- Author
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Omayma A. Eldahshan
- Subjects
Natural products ,lcsh:Therapeutics. Pharmacology ,Traditional medicine ,Building unit ,lcsh:RM1-950 ,medicine ,Cancer ,hepatoprotective ,General Medicine ,Biology ,medicine.disease ,Natural (archaeology) - Abstract
Egypt holds a unique position in the epidemiology of liver diseases and cancer. We, here, shed the light on some important plant extracts and pure compounds that could be used as a new building unit for treatment of liver diseases.
- Published
- 2017
19. Rhoifolin; A Potent Antiproliferative Effect on Cancer Cell Lines
- Author
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Omayma A. Eldahshan
- Subjects
biology ,General Medicine ,biology.organism_classification ,Vinblastine ,Hep G2 ,HeLa ,chemistry.chemical_compound ,Rhoifolin ,chemistry ,Apigenin ,Cancer cell ,medicine ,Cancer research ,Cytotoxic T cell ,Viability assay ,medicine.drug - Abstract
Aims: To investigate the cytotoxic activity of rhoifolin against different cancer cell lines. Study Design: Isolation, identification and cytotoxic activity evaluation. Place and Duration of Study: Faculty of Pharmacy, Ain Shams University and Al-Azhar University, between October, 2010 and January, 2011. Methodology: Rhoifolin, Apigenin 7-O-β neohesperidoside was isolated in a copious amount from the leaves of Chorisia crispiflora (Bombaceae). Its identity was unambiguously confirmed via different spectroscopic methods (UV, 1HNMR, 13CNMR and HMBC) and viability assay test was used to evaluate its cytotoxic activity. Results: It exhibited potent anticancer activities, nearly similar to that of vinblastine, when evaluated against human epidermoid larynex (Hep 2) and human cervical (HeLa) carcinoma cell lines. Promising activities were also obtained against hepatocellular (Hep G2), colon (HCT-116) and fetal human lung fibroblast (MRC-5) carcinoma cell lines. A unique effect of rhoifolin was in having no cytotoxic activity against healthy normal cells (Vero cells) which indicates a high selectivity of this compound. Conclusion: The findings of this study showed that rhoifolin could be used as an ideal anticancer agent. It discriminates between cancerous and non cancerous cell as it kills only the former one. So the side effects which may appear during chemotherapy could be overcome.
- Published
- 2013
20. Chemical Composition of Aqueous Ethanol Extract ofLuffa cylindricaLeaves and Its Effect on Representation of Caspase-8, Caspase-3, and the Proliferation Marker Ki67 in Intrinsic Molecular Subtypes of Breast Cancerin Vitro
- Author
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Amany M. Hilal, Mohamed Ashour, Omayma A. Eldahshan, Ibrahim M Abdel-Salam, and Abeer M. Ashmawy
- Subjects
0301 basic medicine ,Apoptosis ,Breast Neoplasms ,Bioengineering ,Caspase 3 ,Pharmacology ,Caspase 8 ,Biochemistry ,Structure-Activity Relationship ,03 medical and health sciences ,0302 clinical medicine ,Breast cancer ,Cell Line, Tumor ,Biomarkers, Tumor ,medicine ,Humans ,Molecular Biology ,Cell Proliferation ,Dose-Response Relationship, Drug ,Ethanol ,Molecular Structure ,Plant Extracts ,Chemistry ,Water ,Cancer ,General Chemistry ,General Medicine ,medicine.disease ,Antineoplastic Agents, Phytogenic ,In vitro ,Plant Leaves ,Ki-67 Antigen ,030104 developmental biology ,Phytochemical ,Cell culture ,030220 oncology & carcinogenesis ,Molecular Medicine ,Female ,Drug Screening Assays, Antitumor ,Luffa - Abstract
Breast cancer constitutes the second most prevalent cancer in Egypt, the problem needs more trends in treatment and treatment development either by regimen modification or introducing new drugs, and the main objective of this study is to screen the effects of the aqueous ethanol herbal extract of Luffa cylindrica leaves on different types of breast cancer cell lines representing different molecular subtypes of the disease. The major active constituents of the extract were tentatively identified by LC/MS which revealed the presence of phenolic compound derivatives and saponin that may be responsible in part for the activity of the extract. The emphasis was laid on the main apoptotic pathways as well as the extract effect on the normal cell line. Results of phytochemical investigation, cell cycle analysis, and molecular analysis of apoptotic and proliferative markers have shown effective anticancer activity against MCF-7, BT-474, and MDA-MB-231 cell lines which represent three subtypes of breast cancer, luminal A, luminal B, and triple negative, respectively. On the other hand, the effects on normal lung fibroblast cell line are less prominent at the dose used for treating breast cancer cell lines.
- Published
- 2018
21. New Methoxyflavone fromCasimiroa sapotaand the Biological Activities of Its Leaves Extract against Lead Acetate Induced Hepatotoxicity in Rats
- Author
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Eman A. Abd El-Ghffar, Hoda G. Hegazy, Nada M Mostafa, and Omayma A. Eldahshan
- Subjects
Male ,Antioxidant ,medicine.medical_treatment ,Dose dependence ,Bioengineering ,medicine.disease_cause ,01 natural sciences ,Biochemistry ,Flavones ,Structure-Activity Relationship ,03 medical and health sciences ,Casimiroa ,0302 clinical medicine ,Healthy control ,Organometallic Compounds ,medicine ,Animals ,Molecular Biology ,chemistry.chemical_classification ,Dose-Response Relationship, Drug ,Molecular Structure ,Traditional medicine ,biology ,Plant Extracts ,Chemistry ,General Chemistry ,General Medicine ,biology.organism_classification ,Rats ,0104 chemical sciences ,Plant Leaves ,010404 medicinal & biomolecular chemistry ,Lead acetate ,030220 oncology & carcinogenesis ,Molecular Medicine ,Chemical and Drug Induced Liver Injury ,After treatment ,Oxidative stress - Abstract
Flavonoids are agents with strong antioxidant properties and ameliorate many diseases associated with oxidative stress. Leaves of Casimiroa sapota were investigated for components and antioxidant/anti-inflammatory activities against lead acetate ((AcO)2 Pb) induced hepatotoxicity in rats. Three groups of male albino rats were administrated orally with vehicle or C. sapota (100 and 200 mg/kg b.w/day) for 28 days; other group was injected with sub-acute dose (100 mg/kg b.w/day) of (AcO)2 Pb. Three protective groups were injected with (AcO)2 Pb (100 mg/kg b.w/day) for 7 days at day 22 after treatment with either C. sapota (100 or 200 mg/kg b.w/day) or silymarin (SILY) for 28 days. We isolated and identified, from C. sapota, a new compound for the 1st time in nature; 5,6,2',3'-tetramethoxyflavone in addition to the rare compound 5,6,3'-trimethoxyflavone (second report of isolation from nature) and the known compound 5,6,2',3',4'-pentamethoxyflavone. There is an improvement in all hemato-biochemical parameters, antioxidant defense system and anti-inflammatory cytokines of protective groups, which received C. sapota in dose dependent manner. The percentage of changes in all parameters measured in (AcO)2 Pb groups that received vehicle, CS100, CS200 or SILY were 109.2, 37.3, 12.5%, and 1.2% compared with the healthy control group. The C. sapota groups confer a better antioxidant activity by preventing oxidative stress and inflammation in (AcO)2 Pb treated rats. The compounds isolated are responsible at least in part for the observed protective effects.
- Published
- 2018
22. Comparison of the Composition and Antimicrobial Activities of the Essential Oils of Green Branches and Leaves of Egyptian Navel Orange (Citrus sinensis (L.) Osbeck var. malesy)
- Author
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Ahmed F. Halim and Omayma A. Eldahshan
- Subjects
Salmonella ,Antifungal Agents ,Gram-positive bacteria ,Sabinene ,Bioengineering ,Microbial Sensitivity Tests ,medicine.disease_cause ,Gram-Positive Bacteria ,01 natural sciences ,Biochemistry ,Aspergillus fumigatus ,chemistry.chemical_compound ,Structure-Activity Relationship ,Botany ,Gram-Negative Bacteria ,medicine ,Oils, Volatile ,Molecular Biology ,Limonene ,biology ,Dose-Response Relationship, Drug ,010405 organic chemistry ,Fungi ,General Chemistry ,General Medicine ,Antimicrobial ,biology.organism_classification ,0104 chemical sciences ,Anti-Bacterial Agents ,010404 medicinal & biomolecular chemistry ,Horticulture ,chemistry ,Molecular Medicine ,Egypt ,Bacteria ,Citrus × sinensis ,Citrus sinensis - Abstract
The essential oils isolated from the leaves and green branches of the Egyptian navel orange trees were analyzed by GC and GC/MS. A total of 33 and 24 compounds were identified from the oils of the leaves and branches accounting for 96.0% and 97.9%, respectively, of the total detected constituents. The major ones were sabinene (36.5; 33.0%), terpinen-4-ol (8.2; 6.2%), δ-3-carene (7.0; 9.4%), limonene (6.8; 18.7%), trans-ocimene (6.7; 6.1%), and β-myrcene (4.5; 4.4%). The antimicrobial activities of both oils were evaluated using the agar-well diffusion method toward three representatives for each of Gram-positive bacteria, Gram-negative bacteria, and fungi. The oil of leaves was more effective as antimicrobial agent than that of the branches. Streptococcus pyogenes, Staphylococcus aureus, Salmonella typhimurium, and Aspergillus fumigatus were the most sensitive bacteria and fungi by the leaves oil.
- Published
- 2015
23. Chemical Composition and Biological Activities of the Essential Oil from Leaves and Flowers ofPulicaria incisasub.candolleana(Family Asteraceae)
- Author
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Riham O. Bakr, Nahla A. Ayoub, Esraa A. Shahat, and Omayma A. Eldahshan
- Subjects
Copaene ,Antifungal Agents ,Sabinene ,Antineoplastic Agents ,Bioengineering ,Flowers ,Microbial Sensitivity Tests ,Gram-Positive Bacteria ,01 natural sciences ,Biochemistry ,Gas Chromatography-Mass Spectrometry ,Pulicaria ,law.invention ,chemistry.chemical_compound ,law ,Gram-Negative Bacteria ,Botany ,Oils, Volatile ,Humans ,Boranes ,Molecular Biology ,Essential oil ,Antiinfective agent ,biology ,010405 organic chemistry ,Terpinen-4-ol ,Hep G2 Cells ,General Chemistry ,General Medicine ,biology.organism_classification ,Anti-Bacterial Agents ,0104 chemical sciences ,Plant Leaves ,010404 medicinal & biomolecular chemistry ,Horticulture ,chemistry ,Molecular Medicine ,Camphene ,Egypt ,Chrysanthenone - Abstract
The composition of the essential oil isolated from leaves and flowers of Pulicaria incisa sub. candolleana E.Gamal-Eldin was analysed by GC and GC-MS. Forty-nine and sixty-eight compounds were identified from the oils of the leaves and flowers accounting for 86.69% and 84.29%, respectively of the total detected constituents. Both leaves and flowers oils were characterized by the high content of each carvotanacetone with 66.01%, 50.87% and chrysanthenone 13.26%, 24.3% respectively. The cytotoxic activity of both essential oils was evaluated against hepatocellular carcinoma cell line HEPG-2, using MTT assay and vinblastine as a reference drug. Leaf oil showed higher activity with IC50 11.4 μg/mL compared with 37.4 μg/mL for flower oil. The antimicrobial activity of both oils was evaluated using agar well diffusion method towards two representatives for each of Gram positive and Gram negative bacteria as well as four representatives for fungi. The minimum inhibitory concentration of both essential oils against bacterial and fungal strains was obtained in the range of 0.49 – 15.63 μg/mL. This article is protected by copyright. All rights reserved.
- Published
- 2017
24. Profile of Volatile Components of Hydrodistilled and Extracted Leaves of Jacaranda acutifolia and their Antimicrobial Activity Against Foodborne Pathogens
- Author
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Omayma A. Eldahshan, Michael Wink, Nada M Mostafa, Abdel Nasser B. Singab, and Mohamed L. Ashour
- Subjects
Pharmacology ,Phenyl acetate ,Bignoniaceae ,Plant Science ,General Medicine ,Biology ,Antimicrobial ,biology.organism_classification ,Microbiology ,law.invention ,Jacaranda ,Penicillin ,Palmitic acid ,chemistry.chemical_compound ,Complementary and alternative medicine ,chemistry ,law ,Drug Discovery ,medicine ,Food science ,Agar diffusion test ,Essential oil ,medicine.drug - Abstract
Volatile constituents of the essential oil and n-hexane extract of Jacaranda acutifolia Humb. and Bonpl. (Bignoniaceae) leaves were determined, and their antimicrobial activities were investigated using an agar diffusion method. The minimum inhibitory concentrations (MIC) were determined and compared with those of standard antibiotics (penicillin, gentamicin and nystatin). The chemical composition of the oils was analyzed by capillary gas chromatography (GLC-FID) and gas chromatography–mass spectrometry (GLC-MS). Thirty-four components, comprising almost 93.8% of the total peak area, were identified in the leaf essential oil. The main components were methyl linolenate (26.7%), 1-octen-3-ol (10.8%), methyl phenyl acetate (9.9%), β-linalool (5.5%) and palmitic acid (4.7%). The n-hexane extract revealed similar oil constituents, but also p-benzoquinone, phenyl acetic acid, resorcinol and homogentisic acid. The oil showed some activity against Staphylococcus aureus and Escherichia coli with MIC values of 2.2 and 2.9 mg/mL, respectively, and moderate activity against Candida albicans, Salmonella typhimurium and Shigella flexneri. The n-hexane extract showed moderate activities against all tested microorganisms, with MIC values ranging from 3.5 to 10.2 mg/mL. The antimicrobial activities of the hydrodistilled and extracted leaves make their local traditional uses rational.
- Published
- 2014
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