52 results on '"Ruihua Zhang"'
Search Results
2. PGC 1α-Mediates Mitochondrial Damage in the Liver by Inhibiting the Mitochondrial Respiratory Chain as a Non-cholinergic Mechanism of Repeated Low-Level Soman Exposure
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Qian Jin, Yi Zhang, Yalan Cui, Meng Shi, Jingjing Shi, Siqing Zhu, Tong Shi, Ruihua Zhang, Xuejun Chen, Xingxing Zong, Chen Wang, and Liqin Li
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Pharmacology ,Pharmaceutical Science ,General Medicine - Published
- 2023
3. Plasma Exosome-Derived microRNAs Profiles in Patients with Serofast Status: A Cross-Sectional Study
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Jinquan Liu, Ruihua Zhang, Tingting Lian, Zuoxi Chen, Rui-Li Zhang, and Qianqiu Wang
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International Journal of General Medicine ,General Medicine - Abstract
Jinquan Liu,1 Ruihua Zhang,1 Tingting Lian,2 Zuoxi Chen,1 Rui-Li Zhang,3 Qianqiu Wang1 1Institute of Dermatology, Chinese Academy of Medical Sciences & Peking Union Medical College, National Center for STD Control, China Centers for Disease Control and Prevention, Nanjing, 210042, Peopleâs Republic of China; 2Department of Dermatology, Zhongshan Hospital, Fudan University, Shanghai, 200032, Peopleâs Republic of China; 3Department of Dermatology, the Second Affiliated Hospital of Nanjing Medical University, Nanjing, 210003, Peopleâs Republic of ChinaCorrespondence: Rui-Li Zhang; Qianqiu Wang, Email reallyvictor@126.com; wangqianqiunj@126.comPurpose: Syphilis is a sexually transmitted bacterial infection caused by Treponema pallidum (T. pallidum), which can lead to chronic morbidity and adverse complications. In clinical practice, serofast status (SF) patients present with clinical symptoms that are very similar to those of healthy individuals or syphilis-cured patients, and often require prolonged follow-up for diagnosis. Currently, there is increasing interest in the potential of plasma exosome-derived miRNA as a biomarker for the detection of infectious diseases. In this study, we aimed to explore the diagnostic potential of miRNA in SF and its possible biological implications.Patients and Methods: Exosome-derived miRNAs were isolated from peripheral plasma samples obtained from 20 patients with secondary syphilis (SS), SF, serologically cured syphilis (SC), and healthy controls (HC), and differentially expressed miRNAs (DEmiRNAs) were identified by microarray analysis. Prediction of potential target genes, functional annotation, gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis were then performed. The expression of selected miRNAs was confirmed in 37 patients by quantitative reverse transcription polymerase chain reaction (RT-qPCR). A receiver operating characteristic (ROC) analysis was performed to evaluate the diagnostic performance of these miRNAs in differentiating syphilis from HC or SC.Results: The expression profile of plasma exosome-derived miRNA was discovered in individuals with SF through microarray analysis. The targeted genes of DEmiRNAs were found to be involved in diverse biological processes according to GO and KEGG analysis, such as regulation of transcription, mitochondria, Golgi, immune system, apoptosis, Ras signaling pathway, etc. Using RT-qPCR validation, miR-1273g-3p, miR-4485-5p, miR-197-3p, and miR-1908-3p showed significant upregulation in patients with SF. These miRNAs exhibited a superior diagnostic ability, either individually or combined, to distinguish SF from SC or HC.Conclusion: The DEmiRNAs in plasma exosomes may play a role in the pathogenesis of SF and have the potential to become a noble and effective diagnostic method.Keywords: serofast status, syphilis, microRNA, exosome, biomarker
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- 2023
4. A Stable Cell Line Co-expressing hTRPV1 and GCaMP6s: A Novel Cell-based Assay For High-throughput screening of hTRPV1 agonists
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Liqin Li, Chen Wang, Jingjing Shi, Xuejun Chen, Yi Zhang, Tong Shi, Ruihua Zhang, Siqing Zhu, and Xingxing Zong
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Organic Chemistry ,Drug Discovery ,General Medicine ,Computer Science Applications - Abstract
Background: Transient receptor potential vanilloid-1 (TRPV1) is a non-selective cation channel capable of integrating various noxious chemical and physical stimuli. Recently, human TRPV1 (hTRPV1) has attracted wide attention from researchers because it is closely related to pain, inflammation, temperature perception, and tumors. Our study was aimed at generating a stable cell line co-expressing hTRPV1 receptor and GCaMP6s calcium indicator protein and, based on this, developing high-throughput screening methods for targeting hTRPV1 agonists. Method: The CHO-hTRPV1-GCaMP6s cell line stably expressing hTRPV1 and GCaMP6s was generated by co-transfection of hTRPV1 and GCaMP6s into Chinese hamster ovary (CHO) cells. The high-throughput screening methods were developed based on detecting the concentration of intracellular calcium ions ([Ca2+]i) by using chemically synthesized dyes and genetically encoded calcium indicator (GECI). Meanwhile, the sensitivity and adaptability of these methods in the evaluation of capsaicinoids were also compared. Results: A stable cell line co-expressing hTRPV1 and GCaMP6s was generated and used to establish a functional high-throughput screening assay based on the measurement of [Ca2+]i by fluorometric imaging plate reader (FLIPR). The GECI exhibited a higher sensitivity and applicability than that of chemically synthesized dyes in detecting the changes in [Ca2+]i induced by capsaicin. The CHO-hTRPV1-GCaMP6s cell line was further used to detect the dose-dependent relationships of various hTRPV1 agonists (comparison of EC50 values: capsaicin (39 ± 1.67 nM) < nonivamide (67 ± 3.05 nM) < piperine (9222 ± 1851 nM)), and this order is consistent with the pharmacological properties of hTRPV1 activation by these agonists. Conclusion: The successful establishment of the CHO-hTRPV1-GCaMP6s cell lines and their application in high-throughput screening of hTRPV1 agonists.
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- 2023
5. Engineering of a TrpR-Based Biosensor for Altered Dynamic Range and Ligand Preference
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Xinyu Gong, Ruihua Zhang, Jian Wang, and Yajun Yan
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5-Hydroxytryptophan ,Repressor Proteins ,Bacterial Proteins ,Escherichia coli ,Tryptophan ,Biomedical Engineering ,Biosensing Techniques ,General Medicine ,Ligands ,Biochemistry, Genetics and Molecular Biology (miscellaneous) ,Transcription Factors - Abstract
Transcriptional factors play a crucial role in regulating cellular functions. Understanding and altering the dynamic behavior of the transcriptional factor-based biosensors will expand our knowledge in investigating biomolecular interactions and facilitating biosynthetic applications. In this study, we characterized and engineered a TrpR-based tryptophan repressor system in
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- 2022
6. Establishment of indirect ELISA method for Salmonella antibody detection from ducks based on PagN protein
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Shaopeng, Hou, Shuyang, Wang, Xinyuan, Zhao, Wei, Li, Jing, Gao, Yanjun, Wang, Ruihua, Zhang, Lingling, Gong, Shijin, Jiang, and Yanli, Zhu
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Ducks ,General Veterinary ,Escherichia coli ,Animals ,Enzyme-Linked Immunosorbent Assay ,General Medicine ,Antibodies, Viral ,Sensitivity and Specificity ,Antibodies, Bacterial ,Recombinant Proteins - Abstract
Background Salmonella as an important food-borne zoonotic bacterial pathogen, infection in ducks is a recessive infection, however, it can also cause high mortality and threat to food safety. Preventing and controlling the infection and transmission of Salmonella in ducks critically require rapid and sensitive detection method. Full-length Salmonella-specific protein PagN was induced and expressed in E.coil BL21 and was purified as an antigen to establish an indirect enzyme-linked immunosorbent assays (iELSA) detection kit. Results The recombinant PagN protein has a molecular weight of 43 kDa containing a His-tag, was recognized by an anti-Salmonella positive serum by Western blot assay. The optimal concentration of PagN as a coating antigen in the iELISA was 1 μg/mL, and the optimal dilution of enzyme-labeled secondary antibody was 1:4000 (0.025 μg/mL). The cutoff OD450 value was established at 0.268. The iELISA kit showed high selectivity since no cross-reaction with E. coli, Staphylococcus aureus and Streptococcus was observed. iELISA method and Dot-blot test were performed on 100 clinical sera samples collected from duck farms, and the actual coincidence rate was 89% (89/100). 613 duck serum samples from 3 different farms were tested using established method and commercial ELISA kit. The concordance between the two methods was 94.1%. Conclusion Anti-PagN based iELISA can serve as a useful tool for diagnosis of Salmonella infection.
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- 2022
7. Exploring the Tunability and Dynamic Properties of MarR-PmarO Sensor System in Escherichia coli
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Xianqiao Wang, Chenyi Li, Yusong Zou, Ning Liu, Ruihua Zhang, Yajun Yan, Tian Jiang, and Jian Wang
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chemistry.chemical_classification ,Transcriptional factor ,Chemistry ,Biomedical Engineering ,Regulator ,Promoter ,General Medicine ,Computational biology ,medicine.disease_cause ,Biochemistry, Genetics and Molecular Biology (miscellaneous) ,Metabolic engineering ,medicine ,Nucleotide ,Escherichia coli ,Biosensor ,Function (biology) - Abstract
Transcriptional factor-based biosensors (TFBs) have been widely used in dynamic pathway control or high-throughput screening. Here, we systematically explored the tunability of a salicylic acid responsive regulator MarR from Escherichia coli aiming to explore its engineering potential. The effect of endogenous MarR in E. coli on the MarR-PmarO biosensor system was investigated. Furthermore, to investigate the function of marO binding boxes in this biosensor system, a series of hybrid promoters were constructed by placing the marO binding boxes in the strong constitutive pL promoter. The engineered hybrid promoters became responsive to MarR and salicylic acid. To further study the influence of each nucleotide in the marO box on MarR binding, we employed dynamic modeling to simulate the interaction and binding energy between each nucleotide in the marO boxes with the corresponding residues on MarR. Guided by the results of the simulation, we introduced mutations to key positions on the hybrid promoters and investigated corresponding dynamic performance. Two promoter variants I12AII4T and I12AII14T that exhibited improved responsive strengths and shifted dynamic ranges were obtained, which can be beneficial for future metabolic engineering research.
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- 2021
8. The kisspeptin-GnIH signaling pathway in the role of zebrafish courtship and aggressive behavior induced by azoxystrobin
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Xuanjun Guo, Ruihua Zhang, Qian Jin, Niannian Cao, Jingjing Shi, Xingxing Zong, Xuejun Chen, Chen Wang, Xuefeng Li, Sen Pang, and Liqin Li
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Health, Toxicology and Mutagenesis ,General Medicine ,Toxicology ,Pollution - Published
- 2023
9. Nonstructural protein 2A2 from Duck hepatitis A virus type 1 inhibits interferon beta production by interaction with mitochondrial antiviral signaling protein and TANK-binding kinase 1
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Nana Sui, Ruihua Zhang, Yue Jiang, Honglei Yu, Guige Xu, Jingyu Wang, Yanli Zhu, Zhijing Xie, Jiaqing Hu, and Shijin Jiang
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General Veterinary ,General Medicine ,Microbiology - Published
- 2023
10. Repeated low-dose exposures to sarin disrupted the homeostasis of phospholipid and sphingolipid metabolism in guinea pig hippocampus
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Liqin Li, Shikun Deng, Ruihua Zhang, Liangcai Song, Jianfu Xu, Meng Shi, Xuejun Chen, Yi Zhang, Tong Shi, Yalan Cui, Jingjing Shi, and Chen Wang
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Male ,0301 basic medicine ,medicine.medical_specialty ,Sarin ,medicine.medical_treatment ,Guinea Pigs ,Spatial Learning ,Phospholipid ,Morris water navigation task ,Toxicology ,Hippocampus ,Guinea pig ,03 medical and health sciences ,chemistry.chemical_compound ,Cognition ,0302 clinical medicine ,Internal medicine ,medicine ,Animals ,Homeostasis ,Hippocampus (mythology) ,Saline ,Phospholipids ,Sphingolipids ,Behavior, Animal ,Dose-Response Relationship, Drug ,business.industry ,General Medicine ,Sphingolipid ,030104 developmental biology ,Endocrinology ,chemistry ,Lipidomics ,Acetylcholinesterase ,Neurotoxicity Syndromes ,Cholinesterase Inhibitors ,business ,Carboxylic Ester Hydrolases ,030217 neurology & neurosurgery - Abstract
Repeated low-level exposure to sarin results to hippocampus dysfunction. Metabonomics involves a holistic analysis of a set of metabolites in an organism in the search for a relationship between these metabolites and physiological or pathological changes. The objective of the present study was to evaluate the effects of repeated exposure to low-level sarin on the metabonomics in hippocampus of a guinea pig model. Guinea pigs were divided randomly into control and sarin treated groups (n = 14). Guinea pigs in the control group received saline; while the sarin-treated group received 0.4×LD50 (16.8 μg/kg) sarin. Daily injections (a total of 14 days) were administered sc between the shoulder blades in a volume of 1.0 ml/kg body weight. At the end of the final injection, 6 animals in each group were chosen for Morris water maze test. The rest guinea pigs (n = 8 for each group) were sacrificed by decapitation, and hippocampus were dissected for analysis. Compared with the control-group, the escape latency in sarin-group was significantly (p
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- 2021
11. Duck hepatitis A virus type 1 transmission by exosomes establishes a productive infection in vivo and in vitro
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Guige, Xu, Hui, Yan, Yanli, Zhu, Zhijing, Xie, Ruihua, Zhang, and Shijin, Jiang
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General Veterinary ,General Medicine ,Microbiology - Abstract
Duck hepatitis A virus type 1 (DHAV-1) infection causes an acute and highly fatal disease in young ducklings. Exosomes are nano-sized small extracellular vesicles secreted by various cells, which participate in intercellular communication and play a key role in the physiological and pathological processes. However, the role of exosomes in DHAV-1 transmission remains unknown. In this study, through RT-PCR, WB analysis and TEM observation, the complete DHAV-1 genomic RNA, partial viral proteins, and virions were respectively identified in the exosomes derived from DHAV-1-infected duck embryo fibroblasts (DEFs). The productive DHAV-1 infection was transmitted by exosomes in DEFs, duck embryos, and ducklings, and high titers of neutralizing antibodies completely blocked DHAV-1 infection but did not significantly neutralize exosome-mediated DHAV-1 infection. To the best of our knowledge, this is the first report that exosome-mediated DHAV-1 infection was resistant to antibody neutralization in vivo and in vitro, which might be an immune evasion mechanism of DHAV-1.
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- 2023
12. Correction to: H3K27 acetylation activated long noncoding RNA RP11-162G10.5 promotes breast cancer progression via the YBX1/GLO1 axis
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Ning Xie, Ruihua Zhang, Zhuofei Bi, Wei Ren, Kaiyun You, Hai Hu, Ying Xu, and Herui Yao
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Cancer Research ,Oncology ,Molecular Medicine ,General Medicine - Published
- 2023
13. Epidemiological investigation on drug resistance of Salmonella isolates from duck breeding farms in Shandong Province and surrounding areas, China
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Xinyuan Zhao, Wei Li, Shaopeng Hou, Yanjun Wang, Shuyang Wang, Jing Gao, Ruihua Zhang, Shijin Jiang, and Yanli Zhu
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China ,Farms ,General Medicine ,Microbial Sensitivity Tests ,Anti-Bacterial Agents ,Integrons ,Ducks ,Salmonella ,Tetracyclines ,Drug Resistance, Multiple, Bacterial ,Animals ,Animal Science and Zoology ,Chickens ,Multilocus Sequence Typing - Abstract
Duck salmonellosis is a common acute septic infectious disease that spreads rapidly, with serious harm to the duck breeding industry and public health. To date, there are few reports about the epidemiological characteristics of drug resistance in Salmonella from ducks. In this study, an epidemiological investigation was conducted on drug resistance of 110 Salmonella strains isolated from multiple duck farms in Shandong Province and surrounding areas, China. The multidrug-resistant (MDR) rate for 110 Salmonella strains was up to 71.82% (79/110), and 12 types of drug resistance genes were detected in all isolates, including β-lactams, aminoglycosides, tetracycline, macrolides, and quinolones resistance genes. Using the multilocus sequence typing (MLST) based on 7 housekeeping genes, 13 various ST types were identified among all strains, and ST19 (32/110, 29.09%) was the primary type. As the dominant serotypes, S. Kottbus and S. Typhimurium were divided into multiple ST types. A total of 6 kinds of plasmid incompatibility groups were carried in the Salmonella strains, of which IncFIIs (29/110, 26.36%) was most prevalent, and the class I integrons were detected in 78.18% (86/110) of strains. Furthermore, we found that some drug resistance genes, plasmid incompatibility groups, and class I integrons coexist in the same strain. This phenomenon indicates that class I integrons and plasmids are important ways for the spread of drug resistance genes. Therefore, the spread of antibiotic resistance in Salmonella had been facilitated, especially erythromycin (108/110, 98.18%), streptomycin (93/110, 84.54%), and tetracycline (53/110, 48.18%). The above research results broadened ideas and provided directions for the transmission mechanism of Salmonella resistance.
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- 2022
14. Coinfection of novel goose parvovirus–associated virus and duck circovirus in feather sacs of Cherry Valley ducks with feather shedding syndrome
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Yupeng Yang, Hanqing Li, Zhijing Xie, Shijin Jiang, Ruihua Zhang, Jingjing Lan, Nana Sui, Caiyu Lian, and Pengfei Li
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Circovirus ,China ,Veterinary medicine ,animal structures ,feather shedding syndrome (FSS) ,animal diseases ,viruses ,Virus ,Parvoviridae Infections ,Viral Proteins ,03 medical and health sciences ,Goose ,feather sac ,Parvovirinae ,biology.animal ,medicine ,Animals ,duck circovirus (DuCV) ,Circoviridae Infections ,music ,Poultry Diseases ,030304 developmental biology ,lcsh:SF1-1100 ,0303 health sciences ,music.instrument ,Goose parvovirus ,biology ,Mortality rate ,Duck circovirus ,novel goose parvovirus–related virus (NGPV) ,0402 animal and dairy science ,virus diseases ,Syndrome ,04 agricultural and veterinary sciences ,General Medicine ,Feathers ,Immunology, Health and Disease ,medicine.disease ,040201 dairy & animal science ,coinfection ,Satellite Viruses ,Feather ,visual_art ,Mutation ,visual_art.visual_art_medium ,Coinfection ,Animal Science and Zoology ,Flock ,lcsh:Animal culture - Abstract
Since 2017, an infectious disease, named feathers shedding syndrome (FSS), has consistently broken out in Cherry Valley ducks in East China. The sick ducks showed the new clinical symptoms of feathers shedding and being plucked off with difficulty after slaughter. The high incidence rate of 20%-70% predominantly happened in ducks with 4-5 weeks age and nearly 40% mortality rate was observed in infected ducks. In order to explore the possible role of novel goose parvovirus-associated virus (NGPV) and duck circovirus (DuCV) in this disease, a total of 540 feather sac samples were collected from sick ducks with FSS. The infection rates of NGPV and DuCV in samples were 82.78% and 78.89% respectively, and the co-infection rate of the two viruses was 70.00 %. Notably, ducks with 4-5 weeks age usually presented obvious and severe FSS in the flocks with high co-detection rate of NGPV and DuCV. Furthermore, nine NGPV strains were isolated from feather sacs and five synchronous amino acid mutations were demonstrated in VP3 protein. These results indicated that co-infection of NGPV and DuCV might play an important role in duck FSS disease.
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- 2020
15. EEG source estimation in a rare patient with cold-induced reflex epilepsy
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Ye Ren, Jun Zhang, Ruihua Zhang, Tapani Ristaniemi, Ao Ting, Qin Zhang, Fengyu Cong, Xiao Shuying, Yuping Wang, and Xiaoli Li
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Male ,Prefrontal Cortex ,Electroencephalography ,Epilepsy, Reflex ,Reflex seizure ,Rhythm ,Reflex Epilepsy ,medicine ,Humans ,Ictal ,Beta Rhythm ,Oxcarbazepine ,Aged ,medicine.diagnostic_test ,business.industry ,General Medicine ,medicine.disease ,Temporal Lobe ,Cold Temperature ,Neurology ,Neurology (clinical) ,business ,Occipital lobe ,Neuroscience ,medicine.drug - Abstract
Temperature-related reflex epilepsy most often takes the form of hot water epilepsy, but very rarely, reflex epilepsy is related to cold temperature. We report a 70-year-old male who had seizures triggered by cold sensations in the body. Four antiepileptic drugs were taken during the drug treatment, and oxcarbazepine was the most effective at stopping the seizures. We implemented clinical seizure induction and obtained EEG data from an interictal period and two complete ictal periods. Source estimation was performed to identify and map the primary sources involved in the seizures on the cortical level. We found that β rhythm appeared on the prefrontal lobes during the whole ictal period. The low-frequency slow δ and θ rhythms, especially the δ rhythm, appeared in the occipital lobe in the early ictal stage and propagated to the right temporal lobe in the mid-late ictal stage. The prefrontal lobe and right temporal lobe were mainly involved in the generation and propagation of the epileptic activities. This study provides a valuable reference for clinical drug therapy and provides insights into the characteristics of the brain activities involved in cold-induced reflex epilepsy. [Published with video sequences].
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- 2020
16. Evidence of possible vertical transmission of duck hepatitis A virus type 1 in ducks
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Shijin Jiang, Zhijing Xie, Xiansheng Zhang, Jingjing Lan, Ruihua Zhang, and Yupeng Yang
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China ,Veterinary medicine ,animal structures ,Biology ,Hepatitis Virus, Duck ,Amino acid mutation ,medicine ,Animals ,Amino Acid Sequence ,Egg drop syndrome ,Phylogeny ,Poultry Diseases ,Duck hepatitis A virus ,Picornaviridae Infections ,General Veterinary ,General Immunology and Microbiology ,Transmission (medicine) ,Embryo ,General Medicine ,medicine.disease ,Pathogenicity ,Infectious Disease Transmission, Vertical ,Ducks ,Hepatitis, Viral, Animal ,embryonic structures ,Flock ,Sequence Alignment ,Horizontal transmission - Abstract
Duck hepatitis A virus (DHAV) causes a highly contagious and acute disease in ducklings younger than 3 weeks of age and spreads rapidly by horizontal transmission to all susceptible ducklings in the flock. To date, there is no evidence of vertical transmission of DHAV-1. In a previous study, we identified a novel DHAV type 1 (DHAV-1) isolate that could infect adult ducks and induce laying drop. In this study, 30 non-embryonated duck eggs and 60 17-day-old embryos were collected from three breeding duck flocks with egg drop syndrome caused by DHAV-1 in China, and 30 17-day-old embryos were randomly selected from the 60 embryos and allowed to hatch. DHAV-1 RNA was detected by RT-PCR in 10 of 30 non-embryonated eggs, 9 of 30 17-day-old embryos, 5 of 7 dead embryos and 5 of 23 newly hatched ducklings. Overall, 29 of 90 (32.2%) eggs and embryos were positive for DHAV-1. Three DHAV-1 strains were isolated from the dead duck embryos of the three breeding duck flocks, respectively. Pathogenicity studies showed that the three DHAV-1 isolates had median embryo lethal doses but were highly pathogenic to healthy ducklings. Compared with the DHAV reference strains, there were two specific amino acid mutation sites (F169 and S220 ) in VP1 of the three isolates. To the best of our knowledge, this is the first report that DHAV-1 is isolated from duck embryos. The findings provide evidence of possible vertical transmission of DHAV-1 from breeding ducks to ducklings.
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- 2020
17. Establishment of a high throughput screening system for GABAA1 modulators in living cells
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Liqin Li, Chen Wang, Yi Zhang, Tong Shi, Xuejun Chen, Ruihua Zhang, Jingjing Shi, Qian Jin, and Jianfu Xu
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Organic Chemistry ,Drug Discovery ,General Medicine ,Computer Science Applications - Abstract
Background: The incidence of sleep disorders is more than 27% in the worldwide, and the development of novel sleep drugs that target GABAA receptors is of great interest. Traditional drug screening methods restrict the discovery of lead compounds, the high-throughput screening system is a powerful means for the lead compounds discovery of sleep drug. Methods: The GABAA1-CHO cell line stably expressing α1β2γ2L was constituted by cotransfection of α1, β2 and γ2L subunits into CHO-T-Rex cells. The high-throughput screening method of membrane potential targeting GABAAR was established and optimized. The optimized method was used to screen the compound library, and the compounds with high activity were obtained. The active compounds were confirmed in vitro by electrophysiological detection technique, and the sleep effects of compounds in vivo were detected by pentobarbital sodium sleep model in mice. Results: A stable cell line expressing human GABAA1 receptor in CHO-T-Rex cells was generated and used to establish a functional high-throughput screening assay based on the measurement of membrane potential changes in living cells by fluorometric imaging plate reader (FLIPR). The assay was further used to detect the dose-effect relationships of tool compounds, the EC50 values of agonist GABA (137.42 ± 26.31 nM), positive allosteric modulator diazepam (3.22 ± 0.73 μM), and antagonist gabazine (0.16 ± 0.04 μM), blocking agents bicuculine (0.47 ± 0.06 μM) and PTX (6.39 ± 1.17 μM). In the meanwhile, the compounds were screened from a compound library (10000) by the membrane potential dye assay. Selected 4 active compounds were further identified for their EC50 values in vitro by electrophysiological method, the EC50 values of 4 compounds were further determined as 1.37 ± 0.43 μM, 0.69 ± 0.17 μM, 0.77 ± 0.16 μM, and 1.62 ± 0.29 μM. Furthermore, the pentobarbital sleep rate and the sleep time of mice pretreated with 4 active compounds by oral administration were significantly increased compared with mice pretreated with a negative control in vivo experiment. Conclusion: We successfully generated a stable CHO cell line expressing human GABAA1 by induced expression strategy which decreased cytotoxicity. Then, developed an efficient membrane potential detection method for high-throughput screening, the assay based on the stable cell line could distinguish different types of GABAA1 modulators, which would be an effective in vitro system to screen the GABAAR-targeted compounds. Compared with the patch clamp electrophysiological detection method, the membrane potential detection method has higher detection flux for compounds and higher detection sensitivity for active compounds.
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- 2021
18. Subacute sarin exposure disrupted the homeostasis of purine and pyrimidine metabolism in guinea pig striatum studied by integrated metabolomic, lipidomic and proteomic analysis
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Meng Shi, Ruihua Zhang, Qian Jin, Yalan Cui, Jingjing Shi, Xuejun Chen, Tong Shi, Yi Zhang, Siqing Zhu, Xingxing Zong, Jianfu Xu, Chen Wang, and Liqin Li
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Proteomics ,Guinea Pigs ,General Medicine ,Toxicology ,Lipids ,Sarin ,Lethal Dose 50 ,Pyrimidines ,Purines ,Lipidomics ,Animals ,Homeostasis ,Neurotoxicity Syndromes ,Chemical Warfare Agents ,Cholinesterase Inhibitors - Abstract
Sarin was used as a chemical weapon due to its high neurotoxicity and mortality. Subacute sarin induced cognitive and behavioral disorder. However, the underlying mechanism is still unclear. Here we offered a multi-omic approach for the analysis of altered metabolites, lipids, and proteins to explore the neurotoxicity of subacute sarin. Guinea pigs were administered between the shoulder blades 16.8 μg/kg of sarin in a volume of 1.0 ml/kg body weight by subcutaneous injection once daily for 14 days. At the end of the final injection, guinea pigs were sacrificed, and striatum were dissected for analysis. A total of 138 different metabolites were identified in the metabolome analysis. Lipids and lipid-like molecules is the largest group (38.41%). For lipidomic analysis, a total of 216 lipids were identified. In proteomic study, over 4300 proteins were identified and quantified. By integrating these enriched components, we demonstrated that the joint pathways disturbed by subacute sarin mainly involving lipid, purine and pyrimidine metabolism in guinea pig striatum. Overall, this study highlights the powerfulness of omics platforms to deepen the understanding of nerve agents caused neurotoxicity.
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- 2021
19. Isolation and characterization of a naturally attenuated novel duck reovirus strain as a live vaccine candidate
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Ruihua Zhang, Zhijing Xie, Yanli Zhu, Shijin Jiang, Hui Yan, and Guige Xu
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China ,Attenuated vaccine ,General Veterinary ,biology ,Strain (chemistry) ,Orthoreovirus, Avian ,Virulence ,Outbreak ,Viral Vaccines ,General Medicine ,Isolation (microbiology) ,Pathogenicity ,Antibodies, Viral ,Vaccines, Attenuated ,Microbiology ,Virology ,Antibodies, Neutralizing ,Reoviridae Infections ,High morbidity ,Ducks ,biology.protein ,Animals ,Antibody ,Poultry Diseases - Abstract
Novel duck reovirus (NDRV) causes high morbidity in ducklings, and recovered ducklings are often remarkably stunted in growth. In this study, four NDRV strains were isolated from the NDRV outbreaks that occurred in different regions of Shandong province, China. The biological characteristics and pathogenicity of the four NDRV strains were elucidated, and the N20 was identified as a naturally attenuated strain. Three-day-old ducklings were immunized with live N20 strain (100 ELD50/duck), and challenged with 104.52 ELD50 of virulent N19 strain at 7 days post immunization. The vaccinated ducklings showed no evidence of clinical signs, gross and histopathological lesions, or loss of body weight, and 100 % protection against the virulent NDRV N19 infection. The NDRV-specific antibodies were generated in the immunized ducklings and could neutralize different NDRV strains. These results indicated that the N20 strain was a promising live attenuated vaccine candidate against highly pathogenic NDRV infection.
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- 2021
20. Identification of a novel missense eya4 mutation causing autosomal dominant non'‘syndromic hearing loss in a chinese family
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Qin Zhang, Xiao Shuying, Jing Qu, Ruihua Zhang, Jun Zhang, and Ao Ting
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Adult ,Male ,Hearing loss ,Mutation, Missense ,Biology ,03 medical and health sciences ,Exon ,symbols.namesake ,Asian People ,otorhinolaryngologic diseases ,medicine ,Humans ,Missense mutation ,Computer Simulation ,Exome ,Family ,Hearing Loss ,Progressive hearing impairment ,Exome sequencing ,Aged ,Genes, Dominant ,Genetics ,Sanger sequencing ,0303 health sciences ,030302 biochemistry & molecular biology ,General Medicine ,Middle Aged ,medicine.disease ,Genetic Loci ,Mutation (genetic algorithm) ,Trans-Activators ,symbols ,Female ,Sensorineural hearing loss ,medicine.symptom ,Software - Abstract
The aim of this study was to identify the novel missense eya4 mutation which cause autosomal dominant non syndromic hearing loss In a Chinese family. Hearing loss is the most common sensory deficit in humans, but the middle-frequency sensorineural hearing loss (MFSNHL) is rare among hereditary non-syndromic hearing loss, and EYA4 is one of the genes reported to be associated with MFSNHL. A genetic analysis of a Chinese family with autosomal dominant non"‘syndromic progressive hearing impairment was conducted and assessed. Targeted exome sequencing, conducted using DNA samples of an affected member in this family, revealed a novel heterozygous missense mutation c.1855T>G in exon 20 of EYA4, causing amino-acid (aa) substitution Gly for Trp at a conserved position aa-619. The p.W619G mutation related to hearing loss in this Chinese family was validated by Sanger sequencing. Bioinformatic analysis confirmed the pathogenic effects of this mutation. We identified the novel missense mutation c.1855T>G (p.W619G) in EYA4 causing autosomal dominant non-syndromic hearing impairment in the selected Chinese family.
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- 2019
21. The research of SARIMA model for prediction of hepatitis B in mainland China
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Daren, Zhao, Huiwu, Zhang, Qing, Cao, Zhiyi, Wang, and Ruihua, Zhang
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China ,Models, Statistical ,Incidence ,Humans ,Seasons ,General Medicine ,Hepatitis B ,Forecasting - Abstract
Hepatitis B virus infection is a major global public health concern. This study explored the epidemic characteristics and tendency of hepatitis B in 31 provinces of mainland China, constructed a SARIMA model for prediction, and provided corresponding preventive measures.Monthly hepatitis B case data from mainland China from 2013 to 2020 were obtained from the website of the National Health Commission of the People's Republic of China. Monthly data from 2013 to 2020 were used to build the SARIMA model and data from 2021 were used to test the model.Between 2013 and 2020, 9,177,313 hepatitis B cases were reported in mainland China. SARIMA(1,0,0)(0,1,1)12 was the optimal model and its residual was white noise. It was used to predict the number of hepatitis B cases from January to December 2021, and the predicted values for 2021 were within the 95% confidence interval.This study suggests that the SARIMA model simulated well based on epidemiological trends of hepatitis B in mainland China. The SARIMA model is a feasible tool for monitoring hepatitis B virus infections in mainland China.
- Published
- 2022
22. Prevalence and Associated Factors of Portopulmonary Hypertension in Patients with Portal Hypertension: A Case-Control Study
- Author
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Xiaoyu Wen, Ruihua Zhang, Yueming Shao, Yu Zhang, Xin Yin, and Tingting Qin
- Subjects
Male ,medicine.medical_specialty ,Atrial enlargement ,Article Subject ,Anemia ,Hypertension, Pulmonary ,education ,behavioral disciplines and activities ,Pericardial effusion ,General Biochemistry, Genetics and Molecular Biology ,03 medical and health sciences ,Electrocardiography ,0302 clinical medicine ,Risk Factors ,Internal medicine ,medicine.artery ,Hypertension, Portal ,medicine ,Prevalence ,Humans ,Portopulmonary hypertension ,General Immunology and Microbiology ,business.industry ,General Medicine ,Hepatology ,Middle Aged ,medicine.disease ,030220 oncology & carcinogenesis ,Case-Control Studies ,Pulmonary artery ,Cardiology ,Portal hypertension ,Medicine ,030211 gastroenterology & hepatology ,Female ,medicine.symptom ,Mitral valve regurgitation ,business ,Research Article - Abstract
Background and Aims. There are few studies on the prevalence and clinical characteristics of portopulmonary hypertension (POPH) in patients with portal hypertension. In addition, invasive right heart catheterization further limits the clinical diagnosis of POPH patients. Methods. From January 2018 to December 2019, 1004 patients with portal hypertension were treated in the Department of Hepatology, the First Hospital of Jilin University. Based on the inclusion and exclusion criteria, 188 patients with portal hypertension were finally included. We collected complete clinical data, laboratory examinations, and imaging examinations. Patients were divided into a POPH group and a non-POPH group based on echocardiographic results. We calculated the prevalence of POPH in patients with portal hypertension. The differences in clinical characteristics of the two groups of patients were compared. Results. The prevalence of POPH in patients with portal hypertension was 2.8%. Among the 188 patients with portal hypertension with fingertip oxygen saturation < 95 % at rest, 28 patients had POPH (12 males and 16 females), with an average age of 63 ± 8 , and 160 patients did not have POPH (110 males, 50 women), with an average age of 59 ± 11 . The proportion of women in the POPH group ( P < 0.01 ) and patients without liver cancer ( P = 0.044 ) was high. Compared to patients without POPH, patients with POPH had lower hemoglobin (related to the severity of anemia, P < 0.01 ), higher creatinine ( P < 0.05 ), and lower partial pressure of oxygen and carbon dioxide ( P < 0.05 ). Patients with POPH had a higher incidence of atrial enlargement, ventricular enlargement, mitral valve regurgitation, tricuspid regurgitation, pulmonary artery widening, pericardial effusion, and aortic regurgitation than those without POPH. The risk of POPH did not increase with the aggravation of the Child-Pugh classification. Conclusion. The prevalence of POPH in patients with portal hypertension is 2.8%. The proportion of women and nonliver cancer in POPH patients was higher than that in non-POPH patients. In addition, the POPH group had higher creatinine and lower hemoglobin, and echocardiography showed that POPH patients had more cardiac structural changes. In patients with portal hypertension, the risk in patients with POPH has nothing to do with the Child-Pugh classification and MELD score.
- Published
- 2021
23. Relationship Between Chronic Conditions and Balance Disorders in Outpatients with Dizziness: A Hospital-Based Cross-Sectional Study
- Author
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Jingtao Bi, Bo Liu, Yiwen Chen, and Ruihua Zhang
- Subjects
Male ,Pediatrics ,medicine.medical_specialty ,Cross-sectional study ,Dizziness ,Clinical Research ,Risk Factors ,Surveys and Questionnaires ,Diabetes mellitus ,Vertigo ,Outpatients ,Prevalence ,medicine ,Humans ,Postural Balance ,Aged ,Balance (ability) ,biology ,business.industry ,Incidence (epidemiology) ,Balance disorders ,General Medicine ,Hospital based ,Middle Aged ,medicine.disease ,biology.organism_classification ,Hospitals ,Cross-Sectional Studies ,Otorhinolaryngology ,Chronic Disease ,Accidental Falls ,Female ,business - Abstract
BACKGROUND Balance dysfunction is common in adult outpatients with dizziness, especially those who are older, which can lead to catastrophic outcomes such as falls. The aim of this study was to investigate the association between chronic conditions and balance disorders in patients with dizziness, especially those who are elderly. MATERIAL AND METHODS A cross-sectional study was conducted in adult outpatients diagnosed with dizziness referred to the Department of Otolaryngology at Beijing Tongren Hospital from September 2017 to August 2018. All of the patients completed a self-administered, structured questionnaire. Demographic data and information on history of chronic conditions were collected and the patients were divided into 2 groups based on whether their balance was normal or abnormal. RESULTS Three hundred and thirty-two patients were included in this study, 168 in the normal balance group and 164 in the abnormal balance group. The incidence of chronic conditions, including hypertension and diabetes, in the abnormal balance group was higher than that in the normal balance group (all P0.05). In subgroup analysis based on age, in those who were aged ≥60 years, the prevalence of chronic diseases was higher in the abnormal balance group than in the normal balance group (P=0.002), while there was no difference in age between the groups with abnormal and normal balance. Hypertension (OR: 2.268; 95%CI: 1.038-4.957; P0.05) was a risk factor for balance disorders in elderly patients rather than those who were younger (P0.05). CONCLUSIONS Our results show that chronic conditions are associated with balance function in older patients with dizziness. Thus, specialists should consider chronic conditions, especially hypertension, in elderly patients with dizziness.
- Published
- 2020
24. Panax notoginseng saponins and their applications in nervous system disorders: a narrative review
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Jing Qu, Xiaokun Geng, Na Xu, Jianliang Zhang, and Ruihua Zhang
- Subjects
0301 basic medicine ,Nervous system ,biology ,business.industry ,Mechanism (biology) ,Active components ,General Medicine ,Disease ,Review Article ,Bioinformatics ,biology.organism_classification ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,medicine.anatomical_structure ,medicine ,Narrative review ,Panax notoginseng ,business ,030217 neurology & neurosurgery - Abstract
Panax notoginseng saponins (PNS), also called "sanqi" in Chinese, are the main active ingredients which are extracted from the root of Panax notoginseng (Burk.) F. H. Chen., and they have been traditionally used as a medicine in China for hundreds of years with magical medicinal value. PNS have varied biological functions, such as anti-inflammatory effects, anti-cancer effects, anti-neurotoxicity, and the prevention of diabetes. Nervous system disorders, a spectrum of diseases originating from the nervous system, have a significant impact on all aspects of patients' lives. Due to the dramatic gains in global life expectancy, the prevalence of nervous system disorders is growing gradually. Even if the mechanism of these diseases is still not clear, they are mainly characterized by neuronal dysfunction and neuronal death. Consequently, it is essential to find measures to slow down or prevent the onset of these diseases. At present, traditional Chinese medicines, as well as their active components, have gained widespread popularity in preventing and treating these diseases because of their merits, especially PNS. In this review, we predominantly address the recent advances in PNS researches and their biological functions, and highlight their applications in nervous system disorders, such as Alzheimer's disease (AD), Parkinson's disease (PD), and stroke.
- Published
- 2020
25. Positive correlation between miR-570 and prognosis of colon cancer: inhibition of cell proliferation and invasion
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Xuan Yang, Ruihua Zhang, Wen Zhang, Yubin Hou, and Guoxiang Jiang
- Subjects
medicine.medical_specialty ,Colorectal cancer ,General Biochemistry, Genetics and Molecular Biology ,law.invention ,Downregulation and upregulation ,law ,Cell Movement ,Internal medicine ,Cell Line, Tumor ,Biomarkers, Tumor ,Medicine ,Humans ,Neoplasm Invasiveness ,Cell Proliferation ,Hematology ,Cell growth ,business.industry ,Proportional hazards model ,Cell migration ,General Medicine ,medicine.disease ,Prognosis ,MicroRNAs ,Cell culture ,Colonic Neoplasms ,Cancer research ,Suppressor ,Neoplasm Recurrence, Local ,business - Abstract
Colon cancer is one of most common cancers. The progression of various cancers is driven by miRNA-570. The role of miRNA-570 in the progression of colon cancer remains unclear. We aimed to investigate the clinical function of miR-570 and its impact on colon cancer cells. We evaluated the expression of miR-570 in colon cancer cells and analyzed its influence on the various clinical parameters. The Kaplan-Meier curve was plotted to understand the clinical role of miR-570. Cox regression analysis was performed to predict the prognostic factors in colon cancer. The Cell Counting Kit-8 was used to investigate the effect of miR-570 on cell proliferation. The transwell migration assay was performed to quantify cell migration and invasion. The quantitative real-time polymerase chain reaction technique was used to analyze the sample system. The results revealed that the level of miR-570 expression in colon cancer tissues and cell lines was low. The abnormal expression of miR-570 was associated with tumor size, extent of differentiation, lymph node metastasis, and tumor-node-metastasis stages. Downregulation of miR-570 indicated poor overall survival (OS), poor relapse-free survival, and unfavorable cancer-specific survival (CSS) rates in patients with colon cancer. The results from Cox regression analysis revealed that miR-570 expression could be used as an independent prognostic biomarker for OS and CSS in colon cancer. Overexpression of miR-570 can potentially result in the inhibition of cell proliferation, migration, and invasion. The results proved that miR-570 could potentially function as a tumor suppressor and a potential prognostic factor in patients with colon cancer.
- Published
- 2020
26. Occurrence and molecular epidemiology of fosA3-bearing Escherichia coli from ducks in Shandong province of China
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Ruihua Zhang, Jingyu Wang, Zhijing Xie, Yanli Zhu, Shijin Jiang, Fengzhi Liu, and Ang Tian
- Subjects
plasmids ,China ,MICROBIOLOGY AND FOOD SAFETY ,genetic environment ,Fosfomycin ,Biology ,fosA3 ,medicine.disease_cause ,SF1-1100 ,beta-Lactamases ,Microbiology ,Plasmid ,Escherichia coli ,Pulsed-field gel electrophoresis ,medicine ,Animals ,Replicon ,Typing ,Gel electrophoresis ,Molecular Epidemiology ,Molecular epidemiology ,General Medicine ,Animal culture ,Anti-Bacterial Agents ,Ducks ,Animal Science and Zoology ,Chickens ,medicine.drug - Abstract
The plasmid-borne fosfomycin resistance gene fosA3 has been identified in Escherichia coli (E. coli) from various animals but has rarely been reported in ducks. In this study, we investigated the fosA3 prevalence and molecular characteristics of fosA3-harboring E. coli strains from ducks in Shandong province of China. In 416 E. coli isolates, 91 (21.88%) were identified as fosA3-bearing strains, and the fosfomycin-resistant phenotype of 88 of the 91 fosA3-harboring strains was successfully transferred to the recipient strains. Seven different genetic structures surrounding the fosA3 gene were detected and two new contexts were discovered among the fosA3-carrying E. coli. Twenty fosA3-harboring isolates and their trans conjugants were randomly selected for pulsed-field gel electrophoresis (PFGE) typing and S1-nuclease PFGE, respectively. The PFGE patterns revealed that the 20 randomly selected fosA3-bearing isolates were not a result of clonal dissemination. S1-PFGE showed that 15 of the 20 randomly selected trans conjugants carried a single plasmid, and these 15 plasmids that harbored fosA3 (55–190 kb) were distributed into the following replicon types: IncF (n = 11), IncI1 (n = 1), IncN (n = 1), untypable (n = 1), and W-FIC (n = 1). Additionally, as vectors for fosA3 in E. coli, F-:A1:B6, N/ST1, IncI1/ST2, W-FIC, and one untypable plasmid had never been reported before. These observations highlighted the importance of ducks as a reservoir for multidrug-resistant fosA3-carrying E. coli.
- Published
- 2022
27. Quantitative Proteomic Analysis Uncovers the Mediation of Endoplasmic Reticulum Stress-Induced Autophagy in DHAV-1-Infected DEF Cells
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Yu Wang, Shaoli Lin, Wenxiang Xue, Zhijing Xie, Honglei Yu, Jingyu Wang, Junhao Chen, Shijin Jiang, Jingjing Lan, and Ruihua Zhang
- Subjects
Proteomics ,0301 basic medicine ,duck hepatitis A virus type 1 ,quantitative proteomics ,Biology ,Hepatitis Virus, Duck ,Article ,Catalysis ,Inorganic Chemistry ,03 medical and health sciences ,0302 clinical medicine ,Downregulation and upregulation ,Western blot ,Heat shock protein ,Autophagy ,medicine ,Animals ,Humans ,Physical and Theoretical Chemistry ,differentially expressed proteins ,Molecular Biology ,Spectroscopy ,Phagosome ,Picornaviridae Infections ,medicine.diagnostic_test ,Endoplasmic reticulum ,Organic Chemistry ,General Medicine ,Endoplasmic Reticulum Stress ,Computer Science Applications ,Cell biology ,030104 developmental biology ,Host-Pathogen Interactions ,Unfolded protein response ,host–pathogen interaction ,ER stress-induced autophagy ,030217 neurology & neurosurgery - Abstract
Autophagy is a tightly regulated catabolic process and is activated in cells in response to stress signals. Despite extensive study, the interplay between duck hepatitis A virus type 1 (DHAV-1) and the autophagy of host cells is not clear. In this study, we applied proteomics analysis to investigate the interaction mechanism between DHAV-1 and duck embryo fibroblast (DEF) cells. In total, 507 differentially expressed proteins (DEPs) were identified, with 171 upregulated proteins and 336 downregulated proteins. The protein expression level of heat shock proteins (Hsps) and their response to stimulus proteins and zinc finger proteins (ZFPs) were significantly increased while the same aspects of ribosome proteins declined. Bioinformatics analysis indicated that DEPs were mainly involved in the &ldquo, response to stimulus&rdquo, the &ldquo, defense response to virus&rdquo, and the &ldquo, phagosome pathway&rdquo, Furthermore, Western blot results showed that the conversion of microtubule-associated protein 1 light chain 3-I (LC3-I) to the lipidation form of LC3-II increased, and the conversion rate decreased when DEF cells were processed with 4-phenylbutyrate (4-PBA). These findings indicated that DHAV-1 infection could cause endoplasmic reticulum (ER) stress-induced autophagy in DEF cells, and that ER stress was an important regulatory factor in the activation of autophagy. Our data provide a new clue regarding the host cell response to DHAV-1 and identify proteins involved in the DHAV-1 infection process or the ER stress-induced autophagy process.
- Published
- 2019
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28. Duck 'beak atrophy and dwarfism syndrome' disease complex: Interplay of novel goose parvovirus-related virus and duck circovirus?
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Ruihua Zhang, Zhijing Xie, J Li, W Wang, Junhao Chen, Jingjing Lan, Shijin Jiang, and Pengfei Li
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Circovirus ,0301 basic medicine ,China ,animal structures ,viruses ,animal diseases ,Dwarfism ,Genome, Viral ,Disease ,Communicable Diseases, Emerging ,Polymerase Chain Reaction ,Virus ,Parvoviridae Infections ,03 medical and health sciences ,Goose ,Parvovirinae ,biology.animal ,medicine ,Animals ,Circoviridae Infections ,music ,Poultry Diseases ,music.instrument ,General Veterinary ,General Immunology and Microbiology ,biology ,Duck circovirus ,Beak ,Gene Amplification ,virus diseases ,General Medicine ,medicine.disease ,biology.organism_classification ,Virology ,Ducks ,030104 developmental biology ,Infectious disease (medical specialty) ,DNA, Viral ,Coinfection ,Atrophy - Abstract
As a newly emerged infectious disease, duck "beak atrophy and dwarfism syndrome (BADS)" disease has caused huge economic losses to waterfowl industry in China since 2015. Novel goose parvovirus-related virus (NGPV) is believed the main pathogen of BADS disease; however, BADS is rarely reproduced by infecting ducks with NGPV alone. As avian circovirus infection causes clinical symptoms similar to BADS, duck circovirus (DuCV) is suspected the minor pathogen of BADS disease. In this study, an investigation was carried out to determine the coinfection of NGPV and DuCV in duck embryos and in ducks with BADS disease. According to our study, the coinfection of emerging NGPV and DuCV was prevalent in East China (Shandong, Jiangsu and Anhui province) and could be vertical transmitted, indicating their cooperative roles in duck BADS disease.
- Published
- 2018
29. Identification of suh gene and evidence for involvement of notch signaling pathway on gonadal differentiation of Yellow River carp (Cyprinus carpio)
- Author
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Xiaolin Ji, Fang Wang, Ruihua Zhang, Qiyan Du, Zhongjie Chang, Yongfang Jia, Wan-wan Zhang, and Tingting Liang
- Subjects
Male ,0301 basic medicine ,endocrine system ,Carps ,Gonad ,Physiology ,Notch signaling pathway ,Aquatic Science ,Biology ,Synteny ,Biochemistry ,Chromosomes ,03 medical and health sciences ,Transcription (biology) ,medicine ,Animals ,Gonads ,Carp ,Gene ,Phylogeny ,Receptors, Notch ,Chromosome Mapping ,Gene Expression Regulation, Developmental ,Genomics ,General Medicine ,biology.organism_classification ,Cell biology ,Sexual dimorphism ,Fishery ,030104 developmental biology ,medicine.anatomical_structure ,Female ,Signal transduction ,Development of the gonads ,Signal Transduction - Abstract
The suh gene is crucial in Notch pathway and regulates mammalian gonad development. In this study, the sequences of suh1 and suh2 genes in Yellow River carp (Cyprinus carpio) were verified. The partial 5'-flanking regions of suh1 and suh2 were analyzed and several potential transcription factor-binding sites were identified. Phylogenetic, gene structure, and chromosome synteny analyses revealed that carp suh1 and suh2 were orthologs and homologous to vertebrate suh. Investigation of the expression profiles of suh1 and suh2 with qPCR showed that these genes were abundant in the brain and gonad of carp, with suh1 exhibiting sexual dimorphism expression pattern in gonad. To study the relationship between gonad differentiation and Notch signaling, primordial gonads were exposed to DAPT, an inhibitor of Notch signaling, in vitro and in vivo. The results revealed a significant downregulation of suh1 and other Notch genes in vitro. In addition, expression of male-biased genes, such as amh, dmrt1, etc., was downregulated, whereas that of female-biased genes, such as foxl2, gdf9, etc., was upregulated. When the primordial gonads were subjected to long-term DAPT exposure, an increased proportion of ovary and delay in testis development were observed. These results suggest that suh gene may have a conservative function between teleosts and mammals. Furthermore, Notch signaling was found to be involved in gonad differentiation in Yellow River carp, and DAPT was noted to inhibit and enhance the expression of male- and female-biased genes, respectively, and induce the increase in number of females.
- Published
- 2017
30. Isolation and characterization of novel goose parvovirus-related virus reveal the evolution of waterfowl parvovirus
- Author
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Shijin Jiang, Shasha Song, Pengfei Li, Zhijing Xie, Shaoli Lin, Ruihua Zhang, Jingjing Lan, D Sun, and Junhao Chen
- Subjects
Models, Molecular ,0301 basic medicine ,China ,Embryo, Nonmammalian ,Sequence alignment ,Genome, Viral ,Genome ,Virus ,Parvoviridae Infections ,03 medical and health sciences ,Goose ,Parvovirinae ,biology.animal ,Animals ,Phylogeny ,Recombination, Genetic ,Whole genome sequencing ,General Veterinary ,General Immunology and Microbiology ,biology ,Bird Diseases ,Parvovirus ,Sequence Analysis, DNA ,General Medicine ,biology.organism_classification ,Biological Evolution ,Virology ,Ducks ,030104 developmental biology ,Capsid ,Viral evolution ,Capsid Proteins ,Sequence Alignment - Abstract
Short beak and dwarfism syndrome (SBDS) has been constantly breaking out in China since 2015. It is caused by a novel goose parvovirus-related virus (NGPV) and can severely restrict the growth of ducks. In this study, seven NGPV stains were isolated from different regions in China between 2015 and 2016. To better understand the correlation between NGPV and goose parvovirus (GPV), we conducted complete genome sequencing and a comprehensive analysis of the NGPV genome. The phylogenetic and alignment analysis showed that NGPV is a branch of GPV, sharing 92.2%-97.1% nucleotide identity with GPV. Compared with classical GPV, five consensus nucleotide mutations in all the seven NGPV isolates and two 14-nucleotide-pair deletions in six NGPV isolates were found in the inverted terminal repeats, twelve and eight synchronous amino acid changes were found in the replication protein and capsid protein of NGPV, respectively, which might be important for viral gene regulation, humoral immune responses, and host transfer. Notably, SDLY1602 was demonstrated a recombinant strain, with the potential major parent GPV vaccine strain 82-0321v and the minor parent GPV wild strain GDaGPV. This is the first report showing that the recombination between two classical GPV strains generated a NGPV strain circulating in nature. This study will advance our understanding of NGPV molecular biology and facilitate to elucidate the evolutionary characteristics of GPV.
- Published
- 2017
31. Establishing an Artificial Pathway for De Novo Biosynthesis of Vanillyl Alcohol in Escherichia coli
- Author
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Jia Wang, Jian Wang, Justin Forrest Rey, Ruihua Zhang, Qipeng Yuan, Zhenya Chen, Xiaolin Shen, and Yajun Yan
- Subjects
0301 basic medicine ,Biomedical Engineering ,Parabens ,Alcohol ,Biochemistry, Genetics and Molecular Biology (miscellaneous) ,03 medical and health sciences ,chemistry.chemical_compound ,Vanillyl alcohol ,Biosynthesis ,Caffeate O-methyltransferase ,Escherichia coli ,Genes, Synthetic ,Shikimate pathway ,Enzyme kinetics ,Benzyl Alcohols ,chemistry.chemical_classification ,Models, Genetic ,biology ,Escherichia coli Proteins ,Gene Expression Regulation, Bacterial ,Methyltransferases ,General Medicine ,030104 developmental biology ,Enzyme ,Metabolic Engineering ,chemistry ,Biochemistry ,biology.protein ,Enzyme promiscuity - Abstract
Vanillyl alcohol is a phenolic alcohol and is used as a flavoring agent in foods and beverages. In this paper, we propose a novel artificial pathway for microbial production of vanillyl alcohol from simple carbon sources. The pathway extends from 4-hydroxybenzoic acid (4-HBA), and needs only three heterologous enzymes, p-hydroxybenzoate hydroxylase (PobA), carboxylic acid reductase (CAR) and caffeate O-methyltransferase (COMT). First, we examined the promiscuous activity of COMT toward 3,4-dihydroxybenzyl alcohol and found a kcat value of 0.097 s–1. Meanwhile, 499.36 mg/L vanillyl alcohol was produced by COMT in vivo catalysis when fed with 1000 mg/L 3,4-dihydroxybenzyl alcohol. In the following experiment, de novo biosynthesis of vanillyl alcohol was carried out and 240.69 mg/L vanillyl alcohol was produced via modular optimization of pathway genes. This work was to date the first achievement for microbial production of vanillyl alcohol. Additionally, the present study demonstrates the application of enz...
- Published
- 2017
32. Toll-like receptor 5 agonist CBLB502 induces radioprotective effects in vitro
- Author
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Jisheng Chen, Chen Wang, Guochao Zhou, Liqin Li, Tong Shi, Hui Jiang, Xiaojing Lu, Xuejun Chen, Ruihua Zhang, and Jianfu Xu
- Subjects
0301 basic medicine ,Agonist ,medicine.diagnostic_test ,Chemistry ,medicine.drug_class ,Biophysics ,General Medicine ,Cell cycle ,Biochemistry ,Flow cytometry ,Cell biology ,03 medical and health sciences ,030104 developmental biology ,Apoptosis ,TLR5 ,medicine ,Inverse agonist ,Viability assay ,Receptor - Abstract
CBLB502 derived from Salmonella flagellin is a novel agonist of Toll-like receptor 5 (TLR5). It has been shown that CBLB502 can exert high radioprotective efficacy on mice and primates from both GI and hematopoietic syndromes during whole-body irradiation with low toxicity and immunogenicity. However, no effective system has been used to investigate the protective effect of CBLB502 against irradiation and the related mechanism in vitro. In this study, we investigated the radioprotective properties of CBLB502 in HEK293-N-T cells constitutively expressing human TLR5 and NF-κB-dependent luciferase. HEK293-N-T cells were treated with different doses of CBLB502 prior to 60Co-γ ray irradiation. After irradiation, cell viability was real-time measured for 4 days by using the real-time cell analysis system. We found that CBLB502 was capable of efficiently maintaining the survival rate of irradiated HEK293-N-T cells. Then apoptotic cell death and cell cycle were detected by flow cytometry. The results showed that CBLB502 pre-treatment could reduce the apoptosis and promote the recovery of irradiated HEK293-N-T cells from G2-phase arrest in a dose-dependent manner. Our data indicated that CBLB502 has a direct radioprotective effect in vitro via anti-apoptosis and promotes cell cycle recovery. The method developed here could be an effective in vitro system to screen other TLR5-target radioprotectants like CBLB502.
- Published
- 2017
33. Protein adduct binding properties of tabun-subtype nerve agents after exposure in vitro and in vivo
- Author
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Ruihua Zhang, Pengcheng Zhao, Runli Gao, Hongmei Wang, Liangliang Wang, Chengxin Pei, Feiyan Fu, Haibo Liu, and Xiaogang Lu
- Subjects
0301 basic medicine ,Male ,Sarin ,Stereochemistry ,Protein Conformation ,Lysine ,Serum Albumin, Human ,Molecular Dynamics Simulation ,Toxicology ,Mass Spectrometry ,03 medical and health sciences ,chemistry.chemical_compound ,Hemoglobins ,Structure-Activity Relationship ,0302 clinical medicine ,Soman ,medicine ,Animals ,Humans ,Chemical Warfare Agents ,Binding site ,Tabun ,Nerve agent ,Binding Sites ,Hydrogen Bonding ,General Medicine ,Human serum albumin ,Blood proteins ,Organophosphates ,030104 developmental biology ,chemistry ,Immunoglobulin G ,Female ,Rabbits ,030217 neurology & neurosurgery ,medicine.drug ,Protein Binding - Abstract
Upon entering the body, nerve agents can bind active amino acid residues to form phosphonylated adducts. Tabun derivatives (O-alkyl-N,N-dialkyl phosphoroamidocyanidates) have strikingly different structural features from other G-series nerve agents, such as sarin and soman. Here, we investigate the binding mechanism for the phosphonylated adducts of nerve agents of tabun derivatives. Binding sites for three tabun derivatives, O-ethyl-N,N- dimethyl phosphoramidocyanidate (GA), O-ethyl-N,N-ethyl(methyl) phosphoramidocyanidate, and O-ethyl-N,N-diethylphosphoramidocyanidate were studied. Quadrupole-orbitrap mass spectrometry (Q-Orbitrap-MS) coupled to proteomics was used to screen adducts between tabun derivatives and albumin, immunoglobulin, and hemoglobin. The results reveal that all three tabun derivatives exhibit robust selectivity to lysine residues, rather than other amino acid residue types. A set of 10 lysine residues on human serum albumin are labeled by tabun derivatives in vitro, with K525 (K*QTALVELVK) and K199 (LK*CASLQK) peptides displaying the most reactivity. Tabun derivatives formed stable adducts on K525 and K414 (K*VPQVSTPTLVEVSR) for at least 7 days and on K351 (LAK*TYETTLEK) for at least 5 days in a rabbit model. Three of these peptides-K525, K414, and K351-have the highest homology with human serum albumin of all 5 lysine residues that bound to examined rabbit blood proteins in vivo. Molecular simulation of the tabun-albumin interaction using structural analysis and molecular docking provided theoretical evidence supporting lysine residue reactivity to phosphonylation by tabun derivatives. K525 has the lowest free binding energy and the strongest hydrogen bonding to human albumin. In summary, these findings identify unique binding properties for tabun derivatives to blood proteins.
- Published
- 2019
34. Verification of soman-related nerve agents via detection of phosphonylated adducts from rabbit albumin in vitro and in vivo
- Author
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Ruihua Zhang, Feiyan Fu, Liqin Li, Xiaogang Lu, Pengcheng Zhao, Chengxin Pei, Hongmei Wang, and Runli Gao
- Subjects
0301 basic medicine ,Health, Toxicology and Mutagenesis ,Lysine ,Soman ,Peptide ,010501 environmental sciences ,Toxicology ,01 natural sciences ,Mass Spectrometry ,03 medical and health sciences ,chemistry.chemical_compound ,In vivo ,medicine ,Animals ,Serum Albumin ,0105 earth and related environmental sciences ,Nerve agent ,chemistry.chemical_classification ,Binding Sites ,Organophosphate ,Albumin ,General Medicine ,In vitro ,030104 developmental biology ,Biochemistry ,chemistry ,Rabbits ,Nerve Agents ,Biomarkers ,medicine.drug ,Chromatography, Liquid - Abstract
A major challenge in organophosphate compound (OP) and OP nerve agent (OPNA) research has been in the identification and utilization of reliable biomarkers for rapid, sensitive, and efficient detection of OP exposure. Albumin has been widely studied as a biomarker for retrospective verification of exposure to OPNAs, including soman (GD), by detecting the phosphonylation of specific amino acid residues. The aim of the present study was to identify binding sites between GD and rabbit serum albumin in vitro and in vivo. A nano-liquid chromatography coupled with a quadrupole-orbitrap mass spectrometry (nLC-Q-Orbitrap-MS) was used to examine the GD-modified adducts of rabbit albumin. A total of 11 GD-modified sites were found in rabbit serum albumin across three experimental models. The following five GD-modified rabbit albumin sites, which were all lysine residues, were established in vivo: K188, K329, K162, K233, and K525. Two of these five lysine residues, K188 in peptide EK*ALISAAQER and K162 in peptide YK*AILTECCEAADK, were stable for at least 7 days in vivo. Molecular simulation of the GD–albumin interaction provided theoretical evidence for reactivity of the identified lysine residues. The findings suggest that these modifiable lysine residues are potential biomarkers of GD exposure for retrospective analysis by Q-Orbitrap-MS.
- Published
- 2019
35. Excessive Oxidative Stress Contributes to Increased Acute ER Stress Kidney Injury in Aged Mice
- Author
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Gary E. Striker, Feng Zheng, Xiaoyan Zhang, Helen Vlassara, Xiaoyan Liu, Lianghu Huang, Zihan Zheng, Ruihua Zhang, and Youfei Guan
- Subjects
0301 basic medicine ,X-Box Binding Protein 1 ,medicine.medical_specialty ,Programmed cell death ,Aging ,Article Subject ,Transgene ,Mice, Transgenic ,Protein Serine-Threonine Kinases ,medicine.disease_cause ,Biochemistry ,Antioxidants ,Kidney Tubules, Proximal ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Internal medicine ,medicine ,Animals ,Inducer ,RNA, Messenger ,lcsh:QH573-671 ,Kidney ,business.industry ,lcsh:Cytology ,Endoplasmic reticulum ,Tunicamycin ,Membrane Proteins ,Cell Biology ,General Medicine ,Acute Kidney Injury ,Endoplasmic Reticulum Stress ,Mice, Inbred C57BL ,Alternative Splicing ,Oxidative Stress ,030104 developmental biology ,medicine.anatomical_structure ,Endocrinology ,chemistry ,030220 oncology & carcinogenesis ,Unfolded protein response ,Unfolded Protein Response ,Female ,business ,Oxidative stress ,Research Article - Abstract
The aged kidney is susceptible to acute injury due presumably to its decreased ability to handle additional challenges, such as endoplasmic reticulum (ER) stress. This was tested by giving tunicamycin, an ER stress inducer, to either old or young mice. Injection of high dose caused renal failure in old mice, not in young mice. Moreover, injection of low dose resulted in severe renal damage in old mice, confirming the increased susceptibility of aged kidney to ER stress. There existed an abnormality in ER stress response kinetics in aged kidney, characterized by a loss of XBP-1 splicing and decreased PERK-eIF2α phosphorylation at late time point. The presence of excessive oxidative stress in aged kidney may play a role since high levels of oxidation increased ER stress-induced cell death and decreased IRE1 levels and XBP-1 splicing. Importantly, treatment with antioxidants protected old mice from kidney injury and normalized IRE1 and XBP-1 responses. Furthermore, older mice (6 months old) transgenic with antioxidative stress AGER1 were protected from ER stress-induced kidney injury. In conclusion, the decreased ability to handle ER stress, partly due to the presence of excessive oxidative stress, may contribute to increased susceptibility of the aging kidney to acute injury.
- Published
- 2019
36. Programming Surface Chemistry with Engineered Cells
- Author
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Felicia Y. Scott, Ruihua Zhang, Keith C. Heyde, Sung-Ho Paek, and Warren C. Ruder
- Subjects
0301 basic medicine ,Streptavidin ,Cell engineering ,Biomedical Engineering ,Biotin ,Biotin synthase ,Biochemistry, Genetics and Molecular Biology (miscellaneous) ,03 medical and health sciences ,chemistry.chemical_compound ,Synthetic biology ,Tissue engineering ,Synthetic gene ,Biotin synthesis ,Escherichia coli ,Gene Regulatory Networks ,Cell Engineering ,Tissue Engineering ,biology ,General Medicine ,Molecular biology ,030104 developmental biology ,chemistry ,Sulfurtransferases ,Biophysics ,biology.protein ,Synthetic Biology - Abstract
We have developed synthetic gene networks that enable engineered cells to selectively program surface chemistry. E. coli were engineered to upregulate biotin synthase, and therefore biotin synthesis, upon biochemical induction. Additionally, two different functionalized surfaces were developed that utilized binding between biotin and streptavidin to regulate enzyme assembly on programmable surfaces. When combined, the interactions between engineered cells and surfaces demonstrated that synthetic biology can be used to engineer cells that selectively control and modify molecular assembly by exploiting surface chemistry. Our system is highly modular and has the potential to influence fields ranging from tissue engineering to drug development and delivery.
- Published
- 2016
37. Novel duck hepatitis A virus type 1 isolates from adult ducks showing egg drop syndrome
- Author
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Jinqiang Zhang, Junhao Chen, Shijin Jiang, Zhijing Xie, Pengfei Li, Jingjing Lan, Yupeng Yang, and Ruihua Zhang
- Subjects
0301 basic medicine ,Veterinary medicine ,China ,animal structures ,Feed consumption ,040301 veterinary sciences ,animal diseases ,viruses ,Oviposition ,Oviducts ,Biology ,Microbiology ,Disease Outbreaks ,0403 veterinary science ,03 medical and health sciences ,Amino acid mutation ,medicine ,Animals ,Egg drop syndrome ,Pathogen ,Duck hepatitis A virus ,Phylogeny ,Poultry Diseases ,General Veterinary ,Laying duck ,virus diseases ,Outbreak ,04 agricultural and veterinary sciences ,General Medicine ,Hepatitis A ,medicine.disease ,030104 developmental biology ,Ducks ,Female ,Flock ,Hepatitis A virus - Abstract
Generally, duck hepatitis A virus type 1 (DHAV-1) only infects young ducklings. Since December 2016, severe outbreaks of duck viral infection with egg drop, feed consumption decline, and ovary-oviduct disease have occurred in some laying duck flocks in Shandong Province of China. DHAV-1 isolated from the affected ducks was confirmed as the causative pathogen of the egg drop. Compared with other DHAV-1 strains, the novel isolate has three special amino acid mutation points in the most variable regions at the C-terminus of VP1. The experimental infection in laying ducks indicated that successful immunization with DHAV-1 vaccine could protect laying duck from infection. To the best of our knowledge, this is the first reported incidence of a severe duck disease outbreak involving egg drop syndrome caused by DHAV-1.
- Published
- 2018
38. Mapping intrinsic functional brain changes and repetitive transcranial magnetic stimulation neuromodulation in idiopathic restless legs syndrome: a resting-state functional magnetic resonance imaging study
- Author
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Yue Hou, Ruihua Zhang, Zhaoyang Huang, Zhengjia Dai, Yuping Wang, Yong He, Chunyan Liu, Shuqin Zhan, Mo Zhang, Zhigang Qi, and Yicong Lin
- Subjects
Adult ,Male ,medicine.medical_specialty ,Brain activity and meditation ,medicine.medical_treatment ,Asymptomatic ,Physical medicine and rehabilitation ,Reference Values ,Restless Legs Syndrome ,mental disorders ,medicine ,Humans ,Restless legs syndrome ,Aged ,Brain Mapping ,Neurotransmitter Agents ,medicine.diagnostic_test ,Motor Cortex ,Brain ,General Medicine ,Middle Aged ,medicine.disease ,Magnetic Resonance Imaging ,Transcranial Magnetic Stimulation ,Neuromodulation (medicine) ,Transcranial magnetic stimulation ,Female ,Occipital Lobe ,Sensorimotor Cortex ,medicine.symptom ,Primary motor cortex ,Psychology ,Functional magnetic resonance imaging ,Neuroscience ,Insula - Abstract
Objective The objectives of this study were, first, to explore differences in brain activity between normal people and idiopathic restless legs syndrome (RLS) patients during asymptomatic periods; and, second, to determine whether administering repetitive transcranial magnetic stimulation (rTMS) to specific cortical regions would reverse any observed differences in brain activity and alleviate patient symptoms. Methods Fifteen idiopathic RLS patients (nine drug-naive patients) and 14 gender- and age-matched healthy controls were enrolled. Resting-state functional magnetic resonance imaging was used to measure the amplitude of low-frequency fluctuations (ALFF) in spontaneous brain activity during asymptomatic periods. Seven patients received high-frequency (5 Hz) rTMS directed toward the leg area of the primary motor cortex. Scores on the International Restless Legs Syndrome Study Group (IRLSSG) Rating Scale and ALFF values were measured before and after treatment. Results Compared with healthy controls, RLS patients showed lower ALFF in the sensorimotor and visual processing regions, and higher ALFF in the insula, parahippocampal and hippocampal gyri, left posterior parietal areas, and brainstem. These results were largely conserved when only drug-naive patients were considered. After rTMS treatment, ALFF in several sensorimotor and visual regions were significantly elevated and IRLSSG Rating Scale scores decreased, indicating improved RLS symptoms. Conclusions High-frequency rTMS delivered to the leg area of the primary motor cortex may raise functional activity in the sensorimotor and occipital regions, leading to improve symptoms in RLS patients. These results provide novel insight into RLS pathophysiology and suggest a potential mechanism for rTMS therapy in idiopathic RLS patients.
- Published
- 2015
39. Hemoglobin discriminates stages of chronic kidney disease in elderly patients
- Author
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Ruihua Zhang, Jie Zheng, Hong Yan, Ying Guo, Ying Chen, Mingzhao Qin, Wei Zhao, Yao Cui, and Mei Li
- Subjects
Cancer Research ,Pathology ,medicine.medical_specialty ,Creatinine ,medicine.diagnostic_test ,Anemia ,business.industry ,Renal function ,Articles ,General Medicine ,Hematocrit ,urologic and male genital diseases ,medicine.disease ,Gastroenterology ,female genital diseases and pregnancy complications ,chemistry.chemical_compound ,Immunology and Microbiology (miscellaneous) ,chemistry ,Internal medicine ,medicine ,Biomarker (medicine) ,Hemoglobin ,Stage (cooking) ,business ,Kidney disease - Abstract
The prevalence of chronic kidney disease (CKD) increases with age, and anemia is known to affect the outcome of subjects with CKD. However, little is known with regard to the associations between metabolic complications and stages of CKD among elderly patients. Thus, the aim of the present study was to investigate the prevalence of CKD in elderly Chinese patients, as well as the associations between stages of CKD and clinically important complications of anemia. In total, 2,258 individuals with CKD, divided into younger (n=989) and elderly (n=1,269) groups, were enrolled in the study between June 2009 and December 2011. The glomerular filtration rate (GFR) was assessed using a 99mTc-DTPA renal dynamic imaging method (modified Gate's method). The levels of serum creatinine (SCr) and hemoglobin (Hb), and the hematocrit (HCT) were measured simultaneously per manufacturer's instructions. In the elderly group, the levels of SCr and proportional ratios were higher, while the GFR, Hb level, HCT and proportional ratios were lower when compared with the values in the younger group. Statistically significant differences were observed in the Hb concentrations when comparing individuals classified with different stages of CKD in the younger and elderly groups. In the younger group, there was no significant difference in the Hb concentrations between the stage 3a and 3b CKD patients. However, in the elderly group, the Hb concentrations were significantly higher in patients classified with stage 3a CKD when compared with those with stage 3b, whose GFR cutoff point was
- Published
- 2015
40. C-terminal 20 residues of ORF3 protein of duck circovirus genotype 2 regulates the nuclear localization and inhibits apoptotic activity of ORF3 protein
- Author
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Yuming Li, Ruihua Zhang, Shijin Jiang, Zhi-Yong Ma, Hua Chen, Zhuan-Chang Wu, Xin Wang, and Dong-Hua Shao
- Subjects
0301 basic medicine ,Circovirus ,Gene Expression Regulation, Viral ,Genotype ,040301 veterinary sciences ,Viral protein ,Nuclear Localization Signals ,Apoptosis ,Genome, Viral ,Biology ,medicine.disease_cause ,Microbiology ,0403 veterinary science ,03 medical and health sciences ,Open Reading Frames ,Viral Proteins ,medicine ,Animals ,Circoviridae Infections ,music ,Gene ,Phylogeny ,chemistry.chemical_classification ,Cell Nucleus ,music.instrument ,General Veterinary ,Point mutation ,Duck circovirus ,04 agricultural and veterinary sciences ,General Medicine ,Subcellular localization ,Molecular biology ,Amino acid ,030104 developmental biology ,Ducks ,chemistry ,DNA, Viral ,Nuclear localization sequence - Abstract
Duck circovirus (DuCV) is divided into genotypes 1 and 2. The DuCV ORF3 protein is a newly identified viral protein with apoptotic activity. In this study, the differences in the gene sequences, subcellular localization, and apoptotic activities of the ORF3 proteins of DuCV genotypes 1 and 2 were analyzed. A T-to-A point mutation at nucleotide 236 (T236A) in the ORF3 gene sequence of DuCV genotype 1 was observed, which generates a premature stop codon (TAG) and resulted in a truncated ORF3 protein. The ORF3 protein of DuCV genotype 2 is 20 amino acids longer at its C-terminus than the truncated ORF3 protein of genotype 1. A variant monopartite-type nuclear localization signal (RRLRTCNCRACRTLK) was identified within the C-terminal region of the ORF3 protein of DuCV genotype 2, which is essential for the nuclear localization of the protein. The 20 C-terminal residues of the DuCV genotype 2 ORF3 protein also inhibits the apoptotic activity of the protein. Our findings provide insight into the biological and functional characteristics of the DuCV ORF3 protein.
- Published
- 2017
41. Regulation of iNOS on Immune Cells and Its Role in Diseases
- Author
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Qingjie Xue, Yingchun Yan, Huabao Xiong, and Ruihua Zhang
- Subjects
0301 basic medicine ,T-Lymphocytes ,T cells ,Nitric Oxide Synthase Type II ,Review ,Catalysis ,lcsh:Chemistry ,Inorganic Chemistry ,03 medical and health sciences ,0302 clinical medicine ,Immune system ,Animals ,Humans ,dendritic cells ,Physical and Theoretical Chemistry ,Laboratory research ,lcsh:QH301-705.5 ,Molecular Biology ,Spectroscopy ,biology ,Chemistry ,Organic Chemistry ,General Medicine ,Dendritic cell ,macrophages ,Computer Science Applications ,Cell biology ,iNOS ,Nitric oxide synthase ,030104 developmental biology ,lcsh:Biology (General) ,lcsh:QD1-999 ,030220 oncology & carcinogenesis ,biology.protein ,Function (biology) - Abstract
In recent years, there have been many studies on the function of nitric oxide synthase (NOS) in experimental animals and humans. This review analyzes and explores the relationship between inducible nitric oxide synthase (iNOS) and T cells, macrophages, and dendritic cell et al. differentiation using data based on laboratory research, highlighting recent NOS laboratory research. Our insights into research prospects and directions are also presented.
- Published
- 2018
42. The combination of apatinib and S-1 for the treatment of advanced gastric cancer in China
- Author
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Yikuan Feng, Ruihua Zhang, Yan Liu, Changchun Zhou, and Kai Zhang
- Subjects
0301 basic medicine ,Oncology ,medicine.medical_specialty ,business.industry ,MEDLINE ,Treatment options ,General Medicine ,Advanced gastric cancer ,law.invention ,Clinical trial ,03 medical and health sciences ,chemistry.chemical_compound ,030104 developmental biology ,0302 clinical medicine ,Pharmacotherapy ,chemistry ,Randomized controlled trial ,law ,030220 oncology & carcinogenesis ,Meta-analysis ,Internal medicine ,Medicine ,Apatinib ,business - Abstract
Background:Apatinib-targeted therapy is considered a promising treatment option for malignancies. This study systematically evaluated the efficacy and safety of the combination of apatinib and S-1 for the treatment of patients with advanced gastric cancer (GC).Methods:Clinical trials were se
- Published
- 2018
43. Design of Energy Saving Lighting System in University Classroom Based on Wireless Sensor Network
- Author
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Yu Liang, Ruihua Zhang, Wei Wang, and Caiqing Xiao
- Subjects
Computer science ,business.industry ,Electrical engineering ,Classroom based ,General Medicine ,Key distribution in wireless sensor networks ,Software ,Lighting control system ,Sensor node ,Embedded system ,Mobile wireless sensor network ,business ,Smart lighting ,Wireless sensor network - Abstract
In order to reach the objective of intelligence and energy saving for university classroom lighting, energy saving lighting control system in university classroom based on wireless sensor network is designed, including design of sensor node and sink, as well as corresponding development of control program and upper-computer software. The system sets single-chip Ameg16 as control center, realizes communication between nodes via nRF24L01 wireless transceiver module, and realizes communication between sink and upper computer via w5100 wireless internet module. It perceives illumination intensity via photoconductor, detects the human body position via infrared pyroelectric sensor, and places the sensor node on the lamp, so the light can be controlled according to position of human body and current illumination intensity, which can realize energy saving to a large extent on condition that lighting requirement is satisfied. The system has low cost, and there is no need to change the original lighting circuit. The light can be turned off by hand, and when multi-media are used for the class, light can keep off even it is dim. In addition, this system has the function of automatic fault report, which is convenient for property maintenance.
- Published
- 2013
44. The correlation of D-dimer levels with patient outcomes in acute ischemic cerebrovascular disease complicating coronary heart disease
- Author
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Xin Ma, Xiaomeng Chen, Xuemei Wang, Yanling Wang, Fanhua Meng, Qi Kong, Adam Hafeez, Huishan Du, and Ruihua Zhang
- Subjects
Male ,medicine.medical_specialty ,Neurology ,Statistics as Topic ,Coronary Disease ,030204 cardiovascular system & hematology ,Severity of Illness Index ,Correlation ,Fibrin Fibrinogen Degradation Products ,03 medical and health sciences ,0302 clinical medicine ,Risk Factors ,Internal medicine ,Severity of illness ,D-dimer ,Medicine ,Humans ,In patient ,cardiovascular diseases ,Aged ,Retrospective Studies ,Aged, 80 and over ,biology ,business.industry ,C-reactive protein ,Retrospective cohort study ,General Medicine ,Recovery of Function ,Middle Aged ,Coronary heart disease ,Cerebrovascular Disorders ,C-Reactive Protein ,Cardiology ,biology.protein ,Female ,Neurology (clinical) ,business ,030217 neurology & neurosurgery - Abstract
To investigate the correlation between nerve function defect, their degree of recovery, and D-dimer levels in patients with acute ischemic cerebrovascular disease (AICVD) complicating coronary heart disease (CHD) in winter.From 1 October, 2014 to 31 December, 2014, we enrolled patients in a consecutive manner with AICVD who were hospitalized in the Department of Neurology, Beijing Luhe hospital, Capital Medical University. The patients were selected that had an occurrence of AICVD within the last 14 days. A total of 151 cases were divided into CHD group (n = 77) or non-CHD group (n = 74) based on a diagnosis of CHD. The risk factors, hematological indices associated with the diseases, and the nerve function defect and recovery degrees were compared between the two groups. Moreover, according to the result of the preliminary analysis of the CHD and non-CHD groups, patients were further divided into two subgroups based on whether their D-dimer levels were higher than 0.5 mg/l or not. Finally, the nerve function defect and recovery degrees in each subgroups were compared in pairs.Among the patients consecutively enrolled, the percentage of the patients with CHD was 50.99% (77/151) and non-CHD patients was 49.01%. On admission, there was no significant difference in NIHSS scores between the CHD and non-CHD groups. However, there was a significant difference between the CHD and non-CHD groups when comparing the NIHSS scores on 14th day and the mRs scores on 90 (±7)th day after the initial onset (p = 0.006, 0.005). The D-dimer levels of AICVD complicating CHD patients were higher than those not complicating CHD patients (p = 0.006). Those AICVD patients that complicating CHD with also elevated D-dimer levels had most severe neurologic function deficits on 14th day and worst neural function recoveries on 90 (±7)th day after onset (p = 0.001,0.001).AICVD patients complicating CHD is very common in clinical practice. The AICVD patients that complicating CHD showed worse outcomes within 90 days after initial onset of stroke. The D-dimer levels of patients with AICVD complicating CHD were higher. Patients in the CHD group, whose D-dimer levels were higher than the normal standard, had worst outcomes. Paying close attention to the stage of the coronary artery disease and indicators of the coagulation-fibrinolysis is beneficial in the optimization of the clinical treatment for AICVD patients. Maybe the results of this study could provide some reference for specific groups of stroke patients to accept anticoagulant therapy.
- Published
- 2016
45. Asynchronism of Thymocyte Development In Vivo and In Vitro
- Author
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Jinping Zhang, Yiwei Chu, Ying Wang, Sidong Xiong, Xian'An Shao, Yanping Gong, Wei Xu, and Ruihua Zhang
- Subjects
medicine.medical_specialty ,T-Lymphocytes ,Cellular differentiation ,CD3 ,Apoptosis ,Thymus Gland ,Biology ,Lymphocyte Activation ,Organ culture ,Mice ,Organ Culture Techniques ,Antigens, CD ,T-Lymphocyte Subsets ,In vivo ,Internal medicine ,Genetics ,medicine ,Animals ,Molecular Biology ,Cells, Cultured ,Cell Proliferation ,Mice, Inbred BALB C ,Cell Differentiation ,Cell Biology ,General Medicine ,Embryo, Mammalian ,Embryonic stem cell ,In vitro ,Cell biology ,Thymocyte ,Endocrinology ,biology.protein ,CD8 - Abstract
Although fetal thymus organ culture (FTOC) has become widely used to investigate T-cell development, the differences between thymocyte development in vivo and in vitro (in FTOC) remain largely unknown. In this study, the viability and numbers of thymocytes recovered from embryonic thymus lobes in different gestation days (gd) mice or from 15 day embryonic thymus lobes cultured for different days in FTOC system were evaluated. The expression of CD3, CD4, CD8, CD95 ligand (CD95L), and CD69 on thymocytes were analyzed by FACS. The results showed that thymocytes, either in vivo or in vitro, could differentiate from double negative (DN) cells to double positive (DP) cells and to single positive (SP) cells. But the number of total thymocytes and the percentage of DP cells in vitro were less than that in vivo, and the expression of CD95L and CD69 on thymocytes in vitro was higher than that in vivo. Our results suggested that although thymocyte development in vitro could recapitulate thymic development in vivo, the proliferation of thymocytes in vitro was less intensive than that in vivo; the differentiation of thymocytes in vitro was delayed compared with that in vivo; and the apoptosis and activation of thymocytes in vitro were higher than that in vivo. In conclusion, FTOC is a useful system for the study of T cell differentiation, but it is necessary to interpret the results from in vitro studies carefully since the thymocyte development in vitro is asynchronous from that in vivo.
- Published
- 2007
46. Integration of target responsive hydrogel with cascaded enzymatic reactions and microfluidic paper-based analytic devices (µPADs) for point-of-care testing (POCT)
- Author
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Huimin Zhang, Zhenyu Lin, Yanli Ma, Xiaofeng Wei, Jiuxing Li, Shasha Jia, Chaoyong James Yang, Ruihua Zhang, Tian Tian, and Zhi Zhu
- Subjects
Paper ,Materials science ,Aptamer ,Point-of-Care Systems ,Microfluidics ,Biomedical Engineering ,Biophysics ,Nanotechnology ,02 engineering and technology ,01 natural sciences ,Sensitivity and Specificity ,Buffer (optical fiber) ,Software portability ,Molecular recognition ,Cocaine ,Lab-On-A-Chip Devices ,Electrochemistry ,Disposable Equipment ,Point of care ,010401 analytical chemistry ,technology, industry, and agriculture ,Reproducibility of Results ,Hydrogels ,General Medicine ,Folding (DSP implementation) ,Equipment Design ,021001 nanoscience & nanotechnology ,0104 chemical sciences ,Equipment Failure Analysis ,Substance Abuse Detection ,Colorimetry ,Naked eye ,Glucan 1,4-alpha-Glucosidase ,0210 nano-technology ,Biotechnology - Abstract
Paper based microfluidics (µPADs) with advantages of portability, low cost, and ease of use have attracted extensive attention. Here we describe a novel method that integrates glucoamylase-trapped aptamer-crosslinked hydrogel for molecular recognition with cascaded enzymatic reactions for signal amplification and a µPAD for portable readout. Upon target introduction, the hydrogel decomposes to release glucoamylase, which catalyzes the hydrolysis of amylose to produce a large amount of glucose. With a simple folding of the µPAD, the sample solution containing glucose product wicks and diffuses in parallel to each test-zone to carry out homogeneous assays, where glucose is used to produce I2 for brown color visualization through multiple enzymatic and chemical cascade reactions. Through color gradient changes based on different concentrations of the target, a semiquantitative assay is achieved by the naked eye, and quantitation can be obtained by handheld devices. Detection of cocaine in buffer and urine was performed to demonstrate the utility of the hydrogel–µPAD system. More importantly, the hydrogel–µPAD system can be extended to the detection of various targets by incorporating the corresponding aptamer into the hydrogel. The hydrogel–µPAD system reported here provides a new platform for portable, disposable and visual detection of a wide range of targets.
- Published
- 2015
47. Microdialysis combined with liquid chromatography-tandem mass spectrometry for the determination of nimodipine in the guinea pig hippocampus
- Author
-
Chen Wang, Shu Li, Ruihua Zhang, Tong Shi, Liqin Li, and Xiaojing Lu
- Subjects
Microdialysis ,Clinical Biochemistry ,Guinea Pigs ,Tandem mass spectrometry ,030226 pharmacology & pharmacy ,01 natural sciences ,Biochemistry ,Hippocampus ,Analytical Chemistry ,03 medical and health sciences ,0302 clinical medicine ,Nitrendipine ,Liquid chromatography–mass spectrometry ,Tandem Mass Spectrometry ,medicine ,Animals ,Nimodipine ,Chromatography ,Chemistry ,010401 analytical chemistry ,Selected reaction monitoring ,Dihydropyridine ,Cell Biology ,General Medicine ,Repeatability ,0104 chemical sciences ,medicine.drug ,Chromatography, Liquid - Abstract
Nimodipine is a dihydropyridine calcium-channel blocker that has been recently shown to be effective on the function of central nervous system. It has been reported that treatment against deficits of learning and memory in animals and human by maintain the calcium homeostasis in hippocampus with nimodipine may be promising therapeutic strategies. A rapid and sensitive liquid chromatography-tandem mass spectrometric method was developed to determination the nimodipine in hippocampus using microdialysis technique. The separation was accomplished on an Agilent Zorbax SB-C18 column (100mm×2.1mm ID, 3.5μm) with the mobile phase composed of methanol-water (80:20, v/v) containing 0.2% formic acid at a flow rate of 0.2ml/min. Multiple reaction monitoring of the precursor-product ion transitions 419→343 for nimodipine and 361→315 nitrendipine (IS) was used for quantitation. The method was sensitive with a lower limit of quantitation (LLOQ) of 0.2ng/ml for nimodipine, with good linearity in the range of 0.2-20ng/ml. All the validation data, such as accuracy, precision, intra- and inter-day repeatability and stability were within the required limits. The method was successfully applied to p harmacokinetic study of the nimodipine in the guinea pig hippocampus.
- Published
- 2015
48. Identification of a conserved neutralizing linear B-cell epitope in the VP1 proteins of duck hepatitis A virus type 1 and 3
- Author
-
Zhijing Xie, Ye Tian, Yinghao Xin, Ruihua Zhang, Guomei Zhou, Shijin Jiang, Junhao Chen, and Shaoli Lin
- Subjects
medicine.drug_class ,viruses ,Molecular Sequence Data ,Monoclonal antibody ,Microbiology ,Neutralization ,Epitope ,Virus ,Hepatitis Virus, Duck ,Antigen ,medicine ,Animals ,Amino Acid Sequence ,Neutralizing antibody ,Antigens, Viral ,Poultry Diseases ,Viral Structural Proteins ,Picornaviridae Infections ,General Veterinary ,biology ,virus diseases ,Antibodies, Monoclonal ,General Medicine ,Virology ,Antibodies, Neutralizing ,Epitope mapping ,Ducks ,Hepatitis, Viral, Animal ,biology.protein ,Epitopes, B-Lymphocyte ,Antibody ,Epitope Mapping - Abstract
Duck virus hepatitis (DVH), mainly caused by duck hepatitis A virus (DHAV), is a severe disease threaten to duck industry and has worldwide distribution. As the major structural protein, the VP1 protein of DHAV is able to induce neutralizing antibody in ducks. In this study, a monoclonal antibody (mAb) 4F8 against the intact DHAV-1 particles was used to identify the possible epitope in the three serotypes of DHAV. The mAb 4F8 had weak neutralizing activities to both DHAV-1 and DHAV-3, and reacted with the conserved linear B-cell epitopes of (75)GEIILT(80) in DHAV-1 VP1 and (75)GEVILT(80) in DHAV-3 VP1 protein, respectively, while not with DHAV-2 VP1. This was the first report about identification of the common conserved neutralizing linear B-cell epitope of DHAV-1 and DHAV-3, which will facilitate understanding of the antigenic structure of VP1 and the serologic diagnosis of DHAV infection.
- Published
- 2015
49. Marine Geoid from Satellite Altimeter Data
- Author
-
Ruihua Zhang, Zheren Xia, Pan Shi, Yuanxi Yang, and Fuqing Peng
- Subjects
Covariance function ,Homogeneity (statistics) ,General Medicine ,Geophysics ,Sea-surface height ,Covariance ,Geodesy ,Physics::Geophysics ,Ocean surface topography ,Geoid ,Undulation of the geoid ,Altimeter ,Physics::Atmospheric and Oceanic Physics ,Geology - Abstract
We compute the deflections of the vertical line over sea from the altimeter data using simple difference, which reduces effectively the influence of the Dynamic Ocean Topography (DOT) and systematic residuals on the deflections of the vertical line. Then, we present a covariance function to approach precisely the marine geoid from the deflections of the vertical line in view of the homogeneity and isotropy of the covariance functions of the anomalous potenial elements. And the determination of the geoid makes it possible to separate precisely the DOT from the sea surface height (SSH). Finally, the global marine geoid and DOT are predicted from the Topex/Poseidon, ERS-1/2 and Geosat altimeter data, which prove that the above-mentioned methods are scientific and reasonable.
- Published
- 2003
50. Evidence of possible vertical transmission of duck circovirus
- Author
-
Zhijing Xie, Xin Wang, Lin-Lin Xia, Ruihua Zhang, Junhao Chen, Shaoli Lin, Li Zhiguo, and Shijin Jiang
- Subjects
Circovirus ,Veterinary medicine ,animal structures ,Embryo, Nonmammalian ,viruses ,animal diseases ,Biology ,Microbiology ,Polymerase Chain Reaction ,law.invention ,Duck embryo ,Single infection ,law ,Animals ,Circoviridae Infections ,music ,Polymerase chain reaction ,Poultry Diseases ,DNA Primers ,Ovum ,music.instrument ,General Veterinary ,Duck circovirus ,virus diseases ,Computational Biology ,Embryo ,General Medicine ,Infectious Disease Transmission, Vertical ,Duplex pcr ,Ducks ,embryonic structures ,DNA, Viral ,Flock ,Co infection - Abstract
To test the hypothesis that duck circovirus (DuCV) may be vertically transmitted from infected breeder ducks to their ducklings, we investigated 120 newly hatched ducklings, 30 dead duck embryos and 80 non-embryonated duck eggs with the duplex polymerase chain reaction (PCR). DuCV DNA was present in 15 newly hatched ducklings, 4 duck embryos and 3 non-embryonated eggs. Four ducklings from two flocks were co-infected by DuCV-1 and DuCV-2, three ducklings from three flocks were DuCV-1 single infection, and eight ducklings from six flocks were DuCV-2 single infection. One duck embryo and one non-embryonated egg were positive for both DuCV-1 and DuCV-2 DNAs, one embryo for DuCV-1 DNA, and two embryos and two non-embryonated eggs for DuCV-2 DNA. The findings provide evidence of possible vertical transmission of DuCV and simultaneous transmission of DuCV-1 and DuCV-2 from breeder ducks to ducklings.
- Published
- 2014
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