1. Gene delivery to tumor cells by cationic polymeric nanovectors coupled to folic acid and the cell-penetrating peptide octaarginine
- Author
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Jie Shen, Qi-Ying Jiang, Qingqing Wang, Lihua Lai, Guping Tang, and Fu-Jian Xu
- Subjects
Materials science ,Polymers ,Genetic Vectors ,Biophysics ,Mice, Nude ,Bioengineering ,Peptide ,Biocompatible Materials ,Cell-Penetrating Peptides ,Gene delivery ,Endocytosis ,Transfection ,Cell Line ,Biomaterials ,chemistry.chemical_compound ,Mice ,Folic Acid ,Cations ,Materials Testing ,Animals ,Humans ,chemistry.chemical_classification ,Polyethylenimine ,Mice, Inbred BALB C ,Molecular Structure ,Gene Transfer Techniques ,Genetic Therapy ,Ligand (biochemistry) ,Cell biology ,Nanostructures ,chemistry ,Biochemistry ,Mechanics of Materials ,Folate receptor ,Ceramics and Composites ,Cell-penetrating peptide ,Oligopeptides - Abstract
Target ligand folic acid (FA) and cell-penetrating peptide octaarginine (R8) were coupled with the gene vectors (PEI 600 -CyD, PC) composed of β-cyclodextrin (β-CyD) and low-molecular-weight polyethylenimine (PEI, Mw 600) to form nanovectors for highly efficient gene delivery to tumor cells. The resultant ternary nanocomplexes of FA-PC/R8-PC/pDNA produced excellent gene transfaction abilities in the folate receptor (FR)-positive tumor cells in vitro and in vivo . The FR-mediated endocytosis and the R8-mediated transmembrane functionality together contributed to the high transfection levels. This study provides a promising means to produce gene nanovectors for in vivo applications.
- Published
- 2011