1. Pseudogenes as competitive endogenous RNAs: target prediction and validation.
- Author
-
Karreth FA, Ala U, Provero P, and Pandolfi PP
- Subjects
- 3' Untranslated Regions, Gene Expression, Gene Silencing, Genes, Reporter, Humans, MicroRNAs genetics, RNA Interference, RNA, Messenger genetics, Reproducibility of Results, Ribonuclease III deficiency, Ribonuclease III genetics, Ribonuclease III metabolism, Computational Biology methods, Genomics methods, Pseudogenes genetics, RNA genetics
- Abstract
Pseudogenes may regulate expression of their parental genes as well as other protein-coding genes through various mechanisms. One such mechanism is the ability to act as competitive endogenous RNA (ceRNA) and participate in microRNA-mediated cross-regulation. Here, we outline how to predict the targets of pseudogene ceRNAs bioinformatically and how to validate them experimentally.
- Published
- 2014
- Full Text
- View/download PDF