24 results on '"Furness, David N"'
Search Results
2. Kinematic Analysis of Shear Displacement as a Means for Operating Mechanotransduction Channels in the Contact Region between Adjacent Stereocilia of Mammalian Cochlear Hair Cells
- Author
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Furness, David N., Zetes, Deborah E., Hackney, Carole M., and Steele, Charles R.
- Published
- 1997
3. Progressive hearing loss and gradual deterioration of sensory hair bundles in the ears of mice lacking the actin-binding protein Eps8L2
- Author
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Furness, David N., Johnson, Stuart L., Manor, Uri, Rüttiger, Lukas, Tocchetti, Arianna, Offenhauser, Nina, Olt, Jennifer, Goodyear, Richard J., Vijayakumar, Sarath, Dai, Yuhai, Hackney, Carole M., Franz, Christoph, Di Fioreehi, Pier Paolo, Masetto, Sergio, Jones, Sherri M., Knipper, Marlies, Holley, Matthew C., Richardson, Guy P., Kachar, Bechara, and Marcotti, Walter
- Published
- 2013
4. Presynaptic maturation in auditory hair cells requires a critical period of sensory-independent spiking activity
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Johnson, Stuart L., Kuhn, Stephanie, Franz, Christoph, Ingham, Neil, Furness, David N., Knipper, Marlies, Steel, Karen P., Adelman, John P., Holley, Matthew C., and Marcotti, Walter
- Published
- 2013
5. miR-96 regulates the progression of differentiation in mammalian cochlear inner and outer hair cells
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Kuhn, Stephanie, Johnson, Stuart L., Furness, David N., Chen, Jing, Ingham, Neil, Hilton, Jennifer M., Steffes, Georg, Lewis, Morag A., Zampini, Valeria, Hackney, Carole M., Masetto, Sergio, Holley, Matthew C., Steel, Karen P., Marcotti, Walter, and King, Mary-Claire
- Published
- 2011
6. The conductance and organization of the TMC1-containing mechanotransducer channel complex in auditory hair cells.
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Fettiplace, Robert, Furness, David N., and Beurg, Maryline
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HAIR cells , *PERMEABILITY measurement , *KNOCKOUT mice , *MISSENSE mutation , *MEMBRANE proteins - Abstract
Transmembrane channel-like protein 1 (TMC1) is thought to form the ion-conducting pore of the mechanoelectrical transducer (MET) channel in auditory hair cells. Using single-channel analysis and ionic permeability measurements, we characterized six missense mutations in the purported pore region of mouse TMC1. All mutations reduced the Ca2+ permeability of the MET channel, triggering hair cell apoptosis and deafness. In addition, Tmc1 p.E520Q and Tmc1 p.D528N reduced channel conductance, whereas Tmc1 p.W554L and Tmc1 p.D569N lowered channel expression without affecting the conductance. Tmc1 p.M412K and Tmc1 p.T416K reduced only the Ca2+ permeability. The consequences of these mutations endorse TMC1 as the pore of the MET channel. The accessory subunits, LHFPL5 and TMIE, are thought to be involved in targeting TMC1 to the tips of the stereocilia. We found sufficient expression of TMC1 in outer hair cells of Lhfpl5 and Tmie knockout mice to determine the properties of the channels, which could still be gated by hair bundle displacement. Single-channel conductance was unaffected in Lhfpl5−/− but was reduced in Tmie−/−, implying TMIE very likely contributes to the pore. Both the working range and half-saturation point of the residual MET current in Lhfpl5−/− were substantially increased, suggesting that LHFPL5 is part of the mechanical coupling between the tip-link and the MET channel. Based on counts of numbers of stereocilia per bundle, we estimate that each PCDH15 and LHFPL5 monomer may contact two channels irrespective of location. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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7. Putative Immunolocalization of the Mechanoelectrical Transduction Channels in Mammalian Cochlear Hair Cells
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Hackney, Carole M., Furness, David N., Benos, Dale J., Woodley, John F., and Barratt, Joan
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- 1992
8. Age‐related changes in the biophysical and morphological characteristics of mouse cochlear outer hair cells.
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Jeng, Jing‐Yi, Johnson, Stuart L., Carlton, Adam J, De Tomasi, Lara, Goodyear, Richard J., De Faveri, Francesca, Furness, David N., Wells, Sara, Brown, Steve D. M., Holley, Matthew C., Richardson, Guy P., Mustapha, Mirna, Bowl, Michael R., and Marcotti, Walter
- Subjects
HAIR cells ,BONE conduction ,ANIMAL models for aging ,OTOACOUSTIC emissions ,SENSORY receptors ,HEARING disorders ,MICE - Abstract
Key points: Age‐related hearing loss (ARHL) is a very heterogeneous disease, resulting from cellular senescence, genetic predisposition and environmental factors (e.g. noise exposure).Currently, we know very little about age‐related changes occurring in the auditory sensory cells, including those associated with the outer hair cells (OHCs).Using different mouse strains, we show that OHCs undergo several morphological and biophysical changes in the ageing cochlea.Ageing OHCs also exhibited the progressive loss of afferent and efferent synapses.We also provide evidence that the size of the mechanoelectrical transducer current is reduced in ageing OHCs, highlighting its possible contribution in cochlear ageing. Outer hair cells (OHCs) are electromotile sensory receptors that provide sound amplification within the mammalian cochlea. Although OHCs appear susceptible to ageing, the progression of the pathophysiological changes in these cells is still poorly understood. By using mouse strains with a different progression of hearing loss (C57BL/6J, C57BL/6NTac, C57BL/6NTacCdh23+, C3H/HeJ), we have identified morphological, physiological and molecular changes in ageing OHCs (9–12 kHz cochlear region). We show that by 6 months of age, OHCs from all strains underwent a reduction in surface area, which was not a sign of degeneration. Although the ageing OHCs retained a normal basolateral membrane protein profile, they showed a reduction in the size of the K+ current and non‐linear capacitance, a readout of prestin‐dependent electromotility. Despite these changes, OHCs have a normal Vm and retain the ability to amplify sound, as distortion product otoacoustic emission thresholds were not affected in aged, good‐hearing mice (C3H/HeJ, C57BL/6NTacCdh23+). The loss of afferent synapses was present in all strains at 15 months. The number of efferent synapses per OHCs, defined as postsynaptic SK2 puncta, was reduced in aged OHCs of all strains apart from C3H mice. Several of the identified changes occurred in aged OHCs from all mouse strains, thus representing a general trait in the pathophysiological progression of age‐related hearing loss, possibly aimed at preserving functionality. We have also shown that the mechanoelectrical transduction (MET) current from OHCs of mice harbouring the Cdh23ahl allele is reduced with age, highlighting the possibility that changes in the MET apparatus could play a role in cochlear ageing. Key points: Age‐related hearing loss (ARHL) is a very heterogeneous disease, resulting from cellular senescence, genetic predisposition and environmental factors (e.g. noise exposure).Currently, we know very little about age‐related changes occurring in the auditory sensory cells, including those associated with the outer hair cells (OHCs).Using different mouse strains, we show that OHCs undergo several morphological and biophysical changes in the ageing cochlea.Ageing OHCs also exhibited the progressive loss of afferent and efferent synapses.We also provide evidence that the size of the mechanoelectrical transducer current is reduced in ageing OHCs, highlighting its possible contribution in cochlear ageing. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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9. Forgotten Fibrocytes: A Neglected, Supporting Cell Type of the Cochlea With the Potential to be an Alternative Therapeutic Target in Hearing Loss.
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Furness, David N.
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FIBROBLASTS ,HAIR cells ,HEARING disorders ,MENIERE'S disease ,COCHLEA ,ACOUSTIC stimulation ,COCHLEA physiology - Abstract
Cochlear fibrocytes are a homeostatic supporting cell type embedded in the vascularized extracellular matrix of the spiral ligament, within the lateral wall. Here, they participate in the connective tissue syncytium that enables potassium recirculation into the scala media to take place and ensures development of the endolymphatic potential that helps drive current into hair cells during acoustic stimulation. They have also been implicated in inflammatory responses in the cochlea. Some fibrocytes interact closely with the capillaries of the vasculature in a way which suggests potential involvement, together with the stria vascularis , also in the blood-labyrinth barrier. Several lines of evidence suggests that pathology of the fibrocytes, along with other degenerative changes in this region, contribute to metabolic hearing loss (MHL) during aging that is becoming recognized as distinct from, and potentially a precursor for, sensorineural hearing loss (SNHL). This pathology may underlie a significant proportion of cases of presbycusis. Some evidence points also to an association between fibrocyte degeneration and Ménière's disease (MD). Fibrocytes are mesenchymal; this characteristic, and their location, make them amenable to potential cell therapy in the form of cell replacement or genetic modification to arrest the process of degeneration that leads to MHL. This review explores the properties and roles of this neglected cell type and suggests potential therapeutic approaches, such as cell transplantation or genetic engineering of fibrocytes, which could be used to prevent this form of presbycusis or provide a therapeutic avenue for MD. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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10. A Tmc1 mutation reduces calcium permeability and expression of mechanoelectrical transduction channels in cochlear hair cells.
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Beurg, Maryline, Barlow, Amanda, Furness, David N., and Fettiplace, Robert
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HAIR cells ,PERMEABILITY ,GENETIC transduction ,MEMBRANE proteins ,KNOCKOUT mice - Abstract
Mechanoelectrical transducer (MET) currents were recorded from cochlear hair cells in mice with mutations of transmembrane channellike protein TMC1 to study the effects onMET channel properties. We characterized a Tmc1 mouse with a single-amino-acid mutation (D569N), homologous to a dominant human deafness mutation. Measurements were made in both Tmc2 wild-type and Tmc2 knockout mice. By 30 d, Tmc1 pD569N heterozygote mice were profoundly deaf, and there was substantial loss of outer hair cells (OHCs). MET current in OHCs of Tmc1 pD569N mutants developed over the first neonatal week to attain a maximum amplitude one-third the size of that in Tmc1 wild-type mice, similar at apex and base, and lacking the tonotopic size gradient seen in wild type. The MET-channel Ca
2+ permeability was reduced 3-fold in Tmc1 pD569N homozygotes, intermediate deficits being seen in heterozygotes. Reduced Ca2+ permeability resembled that of the Tmc1 pM412K Beethoven mutant, a previously studied semidominant mouse mutation. The MET channel unitary conductance, assayed by single-channel recordings and by measurements of current noise, was unaffected in mutant apical OHCs. We show that, in contrast to the Tmc1 M412K mutant, there was reduced expression of the TMC1 D569N channel at the transduction site assessed by immunolabeling, despite the persistence of tip links. The reduction inMET channel Ca2+ permeability seen in both mutants may be the proximate cause of hair-cell apoptosis, but changes in bundle shape and protein expression in Tmc1 D569N suggest another role for TMC1 apart from forming the channel. [ABSTRACT FROM AUTHOR]- Published
- 2019
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11. Spatiotemporal changes in the distribution of LHFPL5 in mice cochlear hair bundles during development and in the absence of PCDH15.
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Mahendrasingam, Shanthini, Fettiplace, Robert, Alagramam, Kumar N., Cross, Ellen, and Furness, David N.
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GENOMES ,GENETICS ,IMMUNOGLOBULINS ,AMINO acids ,HAIR cells - Abstract
Mechanosensory transduction by vertebrate hair cells depends on a protein complex at the tips of shorter stereocilia associated with mechanoelectrical transduction channels activated by tip links in the hair bundle. In mammalian hair cells, this complex includes transmembrane channel-like protein subunit 1 (TMC1), lipoma HMGIC fusion partner-like 5 protein (LHFPL5) and protocadherin 15 (PCDH15), a lower-end component of the tip link. TMC1 interacts with LHFPL5 and PCDH15 but how the complex develops to maturity, and the relationships between these proteins, remains uncertain. Here we evaluate the spatiotemporal development of LHFPL5 distributions in mouse cochlear hair bundles by immunofluorescence and immunogold transmission electron microscopy, from postnatal day 0 (P0) through P21 in wild type and PCDH15-deficient mice. At P0, hair bundles contain many short microvilli-like processes which we term unranked stereocilia, and a subset of lengthening rows, adjacent to a kinocilium. LHFPL5 is distributed throughout the bundle, including on stereocilia tips and the kinocilium. At P3, 4-to-6 rows of ranked stereocilia are evident, total LHFPL5 expression peaks, and LHFPL5 is localised to ranked stereocilia tips of all rows and to lower shaft/ankle links. By P12, the bundle has a mature pattern with 3 ranked rows but virtually no unranked stereocilia or kinocilium; LHFPL5 expression has declined and become restricted to the tips of shorter stereocilia. Throughout development from P0, expression of LHFPL5 is greater overall on apical than basal bundles, but there is, on average, an equal amount of labelling per labelled tip. In P3 mice lacking PCDH15, LHFPL5 labelling is not at the tips but is primarily on unranked stereocilia and lower lateral links. These data show that LHFPL5 is already present in the MET apparatus at P0 but requires PCDH15 at P3 to remain there. Shaft/ankle link localisation suggests it interacts with link proteins other than PCDH15. [ABSTRACT FROM AUTHOR]
- Published
- 2017
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12. Local mechanisms for loud sound-enhanced aminoglycoside entry into outer hair cells.
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Hongzhe Li, Kachelmeier, Allan, Furness, David N., and Steyger, Peter S.
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AMINOGLYCOSIDES ,HAIR cells ,QUALITY of life ,OTOTOXICITY ,GENTAMICIN - Abstract
Loud sound exposure exacerbates aminoglycoside ototoxicity, increasing the risk of permanent hearing loss and degrading the quality of life in affected individuals. We previously reported that loud sound exposure induces temporary threshold shifts (TTS) and enhances uptake of aminoglycosides, like gentamicin, by cochlear outer hair cells (OHCs). Here, we explore mechanisms by which loud sound exposure and TTS could increase aminoglycoside uptake by OHCs that may underlie this form of ototoxic synergy. Mice were exposed to loud sound levels to induce TTS, and received fluorescently-tagged gentamicin (GTTR) for 30 min prior to fixation. The degree of TTS was assessed by comparing auditory brainstem responses (ABRs) before and after loud sound exposure. The number of tip links, which gate the GTTR-permeant mechanoelectrical transducer (MET) channels, was determined in OHC bundles, with or without exposure to loud sound, using scanning electron microscopy. We found wide-band noise (WBN) levels that induce TTS also enhance OHC uptake of GTTR compared to OHCs in control cochleae. In cochlear regions with TTS, the increase in OHC uptake of GTTR was significantly greater than in adjacent pillar cells. In control mice, we identified stereociliary tip links at ~50% of potential positions in OHC bundles. However, the number of OHC tip links was significantly reduced in mice that received WBN at levels capable of inducing TTS. These data suggest that GTTR uptake by OHCs during TTS occurs by increased permeation of surviving, mechanically-gated MET channels, and/or non-MET aminoglycoside-permeant channels activated following loud sound exposure. Loss of tip links would hyperpolarize hair cells and potentially increase drug uptake via aminoglycoside-permeant channels expressed by hair cells. The effect of TTS on aminoglycoside-permeant channel kinetics will shed new light on the mechanisms of loud sound-enhanced aminoglycoside uptake, and consequently on ototoxic synergy. [ABSTRACT FROM AUTHOR]
- Published
- 2015
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13. CONNECTIONS BETWEEN STEREOCILIARY ROOTLETS AND LATERAL WALL: A POSSIBLE ROUTE FOR INTERACTIONS BETWEEN BUNDLE AND PRESTIN BASED COCHLEAR AMPLIFICATION?
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FURNESS, DAVID N., MAHENDRASINGAM, SHANTHINI, and HACKNEY, CAROLE M.
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AUDIO amplifiers ,COCHLEA ,HAIR cells ,TRANSMISSION electron microscopy ,SCANNING electrochemical microscopy - Published
- 2009
14. PRESTIN DISTRIBUTION IN RAT OUTER CELLS - AN ULTRASTRUCTURAL STUDY.
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MAHENDRASINGAM, SHANTHINI, FURNESS, DAVID N., FETTIPLACE, ROBERT, and HACKNEY, CAROLE M.
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HAIR cells ,COCHLEA ,CORTI'S organ ,IMMUNOGOLD labeling ,HEARING - Published
- 2009
15. The composition and role of cross links in mechanoelectrical transduction in vertebrate sensory hair cells.
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Hackney, Carole M. and Furness, David N.
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HAIR cells , *CELLULAR signal transduction , *CADHERINS , *GENETICS of deafness , *MICROVILLI , *NEUROTRANSMITTER receptors , *MOLECULAR biology - Abstract
The key components of acousticolateralis systems (lateral line, hearing and balance) are sensory hair cells. At their apex, these cells have a bundle of specialized cellular protrusions, which are modified actin-containing microvilli, connected together by extracellular filaments called cross links. Stereociliary deflections open nonselective cation channels allowing ions from the extracellular environment into the cell, a process called mechanoelectrical transduction. This produces a receptor potential that causes the release of the excitatory neurotransmitter glutamate onto the terminals of the sensory nerve fibres, which connect to the cell base, causing nerve signals to be sent to the brain. Identification of the cellular mechanisms underlying mechanoelectrical transduction and of some of the proteins involved has been assisted by research into the genetics of deafness, molecular biology and mechanical measurements of function. It is thought that one type of cross link, the tip link, is composed of cadherin 23 and protocadherin 15, and gates the transduction channel when the bundle is deflected. Another type of link, called lateral (or horizontal) links, maintains optimal bundle cohesion and stiffness for transduction. This Commentary summarizes the information currently available about the structure, function and composition of the links and how they might be relevant to human hearing impairment. [ABSTRACT FROM AUTHOR]
- Published
- 2013
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16. The development, distribution and density of the plasma membrane calcium ATPase 2 calcium pump in rat cochlear hair cells.
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Chen, Qingguo, Mahendrasingam, Shanthini, Tickle, Jacqueline A., Hackney, Carole M., Furness, David N., and Fettiplace, Robert
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CELL membranes ,ADENOSINE triphosphatase ,CALCIUM pumps ,LABORATORY rats ,HAIR cells ,HOMEOSTASIS ,IMMUNOFLUORESCENCE - Abstract
Calcium is tightly regulated in cochlear outer hair cells (OHCs). It enters mainly via mechanotransducer (MT) channels and is extruded by the plasma membrane calcium ATPase (PMCA)2 isoform of the PMCA, mutations in which cause hearing loss. To assess how pump expression matches the demands of Ca
2+ homeostasis, the distribution of PMCA2 at different cochlear locations during development was quantified using immunofluorescence and post-embedding immunogold labeling. The PMCA2 isoform was confined to stereociliary bundles, first appearing at the base of the cochlea around post-natal day (P)0 followed by the middle and then the apex by P3, and was unchanged after P8. The developmental appearance matched the maturation of the MT channels in rat OHCs. High-resolution immunogold labeling in adult rats showed that PMCA2 was distributed along the membranes of all three rows of OHC stereocilia at similar densities and at about a quarter of the density in inner hair cell stereocilia. The difference between OHCs and inner hair cells was similar to the ratio of their MT channel resting open probabilities. Gold particle counts revealed no difference in PMCA2 density between low- and high-frequency OHC bundles despite larger MT currents in high-frequency OHCs. The PMCA2 density in OHC stereocilia was determined in low- and high-frequency regions from calibration of immunogold particle counts as 2200/μm2 from which an extrusion rate of ∼200 ions/s per pump was inferred. The limited ability of PMCA2 to extrude the Ca2+ load through MT channels may constitute a major cause of OHC vulnerability and high-frequency hearing loss. [ABSTRACT FROM AUTHOR]- Published
- 2012
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17. The Mechanosensory Structure of the Hair Cell Requires Clarin-1, a Protein Encoded by Usher Syndrome III Causative Gene.
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Ruishuang Geng, Melki, Sami, Chen, Daniel H.-C., Tian, Guilian, Furness, David N., Tomoko Oshima-Takago, Neef, Jakob, Moser, Tobias, Askew, Charles, Horwitz, Geoff, Holt, Jeffrey R., Yoshikazu Imanishi, and Alagramam, Kumar N.
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HAIR cells ,USHER'S syndrome ,GENETIC mutation ,TETRASPANIN ,IMMUNOHISTOCHEMISTRY ,SYNAPSES ,LABORATORY mice - Abstract
Mutation in the clarin-1 gene (Clrn1) results in loss of hearing and vision in humans(Ushersyndrome III), but the role of clarin-1 in the sensory hair cells is unknown. Clarin-1 is predicted to be a four transmembrane domain protein similar to members of the tetraspanin family. Mice carrying null mutation in the clarin-1 gene (Clrn1
-/- )show loss of hair cell function and a possible defect in ribbon synapse. We investigated the role of clarin-1 using various in vitro and in vivo approaches. We show by immunohistochemistry and patch-clamp recordings of Ca2+ currents and membrane capacitance from inner hair cells that clarin-1 is not essential for formation or function of ribbon synapse. However, reduced cochlear microphonic potentials, FM1-43 [N-(3-triethylammoniumpropyl)-4-(4-(dibutylamino)styryl) pyridinium dibromide] loading, and transduction currents pointed to diminished cochlear hair bundle function in Clrn1-/- mice. Electron microscopy of cochlear hair cells revealed loss of some tall stereocilia and gaps in the v-shaped bundle, although tip links and staircase arrangement of stereocilia were not primarily affected by Clrn1-/- mutation. Human clarin-1 protein expressed in transfected mouse cochlear hair cells localized to the bundle; however, the pathogenic variant p.N48K failed to localize to the bundle. The mouse model generated to study the in vivo consequence of p.N48K in clarin-1 (Clrn1N48K ) supports our in vitro and Clrn1-/- mouse data and the conclusion that CLRN1 is an essential hair bundle protein. Furthermore, the ear phenotype in the Clrn1N48K mouse suggests that it is a valuable model for ear disease in CLRN1N48K , the most prevalent Usher syndrome III mutation in North America. [ABSTRACT FROM AUTHOR]- Published
- 2012
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18. Eps8 Regulates Hair Bundle Length and Functional Maturation of Mammalian Auditory Hair Cells.
- Author
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Zampini, Valeria, Rüttiger, Lukas, Johnson, Stuart L., Franz, Christoph, Furness, David N., Waldhaus, Jörg, Hao Xiong, Hackney, Carole M., Holley, Matthew C., Offenhauser, Nina, Di Fiore, Pier Paolo, Knipper, Marlies, Masetto, Sergio, and Marcotti, Walter
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EPIDERMAL growth factor ,HAIR cells ,COCHLEA ,MAMMALS ,AUDITORY perception ,GENETIC transduction ,ACTIN ,DEAFNESS - Abstract
Hair cells of the mammalian cochlea are specialized for the dynamic coding of sound stimuli. The transduction of sound waves into electrical signals depends upon mechanosensitive hair bundles that project from the cell's apical surface. Each stereocilium within a hair bundle is composed of uniformly polarized and tightly packed actin filaments. Several stereociliary proteins have been shown to be associated with hair bundle development and function and are known to cause deafness in mice and humans when mutated. The growth of the stereociliar actin core is dynamically regulated at the actin filament barbed ends in the stereociliary tip. We show that Eps8, a protein with actin binding, bundling, and barbed-end capping activities in other systems, is a novel component of the hair bundle. Eps8 is localized predominantly at the tip of the stereocilia and is essential for their normal elongation and function. Moreover, we have found that Eps8 knockout mice are profoundly deaf and that IHCs, but not OHCs, fail to mature into fully functional sensory receptors. We propose that Eps8 directly regulates stereocilia growth in hair cells and also plays a crucial role in the physiological maturation of mammalian cochlear IHCs. Together, our results indicate that Eps8 is critical in coordinating the development and functionality of mammalian auditory hair cells. [ABSTRACT FROM AUTHOR]
- Published
- 2011
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19. Mutations in Protocadherin 15 and Cadherin 23 Affect Tip Links and Mechanotransduction in Mammalian Sensory Hair Cells.
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Alagramam, Kumar N., Goodyear, Richard J., Ruishuang Geng, Furness, David N., van Aken52, Alexander F. J., Marcotti, Walter, Kros, Corné J., and Richardson, Guy P.
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CADHERINS ,HAIR cells ,EPITHELIAL cells ,AMINOGLYCOSIDES ,SCANNING electron microscopy ,GENTAMICIN - Abstract
Immunocytochemical studies have shown that protocadherin-15 (PCDH15) and cadherin-23 (CDH23) are associated with tip links, structures thought to gate the mechanotransducer channels of hair cells in the sensory epithelia of the inner ear. The present report describes functional and structural analyses of hair cells from Pcdh15
av3J (av3J), Pcdh15av6J (av6J) and Cdh23v2J (v2J) mice. The av3J and v2J mice carry point mutations that are predicted to introduce premature stop codons in the transcripts for Pcdh15 and Cdh23, respectively, and av6J mice have an in-frame deletion predicted to remove most of the 9th cadherin ectodomain from PCDH15. Severe disruption of hair-bundle morphology is observed throughout the earlypostnatal cochlea in av3J/av3J and v2J/v2J mice. In contrast, only mild-to-moderate bundle disruption is evident in the av6J/ av6J mice. Hair cells from av3J/av3J mice are unaffected by aminoglycosides and fail to load with [3 H]-gentamicin or FM1-43, compounds that permeate the hair cell's mechanotransducer channels. In contrast, hair cells from av6J/av6J mice load with both FM1-43 and [3 H]-gentamicin, and are aminoglycoside sensitive. Transducer currents can be recorded from hair cells of all three mutants but are reduced in amplitude in all mutants and have abnormal directional sensitivity in the av3J/av3J and v2J/v2J mutants. Scanning electron microscopy of early postnatal cochlear hair cells reveals tip-link like links in av6J/av6J mice, substantially reduced numbers of links in the av3J/av3J mice and virtually none in the v2J/v2J mice. Analysis of mature vestibular hair bundles reveals an absence of tip links in the av3J/av3J and v2J/v2J mice and a reduction in av6J/av6J mice. These results therefore provide genetic evidence consistent with PCDH15 and CDH23 being part of the tip-link complex and necessary for normal mechanotransduction. [ABSTRACT FROM AUTHOR]- Published
- 2011
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20. Synaptotagmin IV determines the linear Ca2+ dependence of vesicle fusion at auditory ribbon synapses.
- Author
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Johnson, Stuart L., Franz, Christoph, Kuhn, Stephanie, Furness, David N., Rüttiger, Lukas, Münkner, Stefan, Rivolta, Marcelo N., Seward, Elizabeth P., Herschman, Harvey R., Engel, Jutta, Knipper, Marlies, and Marcotti, Walter
- Subjects
HAIR cells ,COCHLEA ,MAMMALS ,SYNAPSES ,NEURAL transmission ,SYNAPTIC vesicles ,RODENTS ,OLFACTORY receptors - Abstract
Mammalian cochlear inner hair cells (IHCs) are specialized for the dynamic coding of continuous and finely graded sound signals. This ability is largely conferred by the linear Ca
2+ dependence of neurotransmitter release at their synapses, which is also a feature of visual and olfactory systems. The prevailing hypothesis is that linearity in IHCs occurs through a developmental change in the Ca2+ sensitivity of synaptic vesicle fusion from the nonlinear (high order) Ca2+ dependence of immature spiking cells. However, the nature of the Ca2+ sensor(s) of vesicle fusion at hair cell synapses is unknown. We found that synaptotagmin IV was essential for establishing the linear exocytotic Ca2+ dependence in adult rodent IHCs and immature outer hair cells. Moreover, the expression of the hitherto undetected synaptotagmins I and II correlated with a high-order Ca2+ dependence in IHCs. We propose that the differential expression of synaptotagmins determines the characteristic Ca2+ sensitivity of vesicle fusion at hair cell synapses. [ABSTRACT FROM AUTHOR]- Published
- 2010
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21. The Dimensions and Composition of Stereociliary Rootlets in Mammalian Cochlear Hair Cells: Comparison between High- and Low-Frequency Cells and Evidence for a Connection to the Lateral Membrane.
- Author
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Furness, David N., Mahendrasingam, Shanthini, Ohashi, Mitsuru, Fettiplace, Robert, and Hackney, Carole M.
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HAIR cells , *CORTI'S organ , *MECHANORECEPTORS , *TROPOMYOSINS , *NEUROSCIENCES - Abstract
The sensory bundle of vertebrate cochlear hair cells consists of actin-containing stereocilia that are thought to bend at their ankle during mechanical stimulation. Stereocilia have dense rootlets that extend through the ankle region to anchor them into the cuticular plate. Because this region may be important in bundle stiffness and durability during prolonged stimulation at high frequencies, we investigated the structure and dimensions of rootlets relative to the stereocilia in apical (low-frequency) and basal (high-frequency) regions of rodent cochleae using light and electron microscopy. Their composition was investigated using postembedding immunogold labeling of tropomyosin, spectrin, β-actin, γ-actin, espin, and prestin. The rootlets have a thick central core that widens at the ankle, and are embedded in a filamentous meshwork in the cuticular plate. Within a particular frequency region, rootlet length correlates with stereociliary height but between regions it changes disproportionately; apical stereocilia are, thus, approximately twice the height of basal stereocilia in equivalent rows, but rootlet lengths increase much less. Some rootlets contact the tight junctions that underlie the ends of the bundle. Rootlets contain spectrin, tropomyosin, and β- and γ-actin, but espin was not detected; spectrin is also evident near the apical and junctional membranes, whereas prestin is confined to the basolateral membrane below the junctions. These data suggest that rootlets strengthen the ankle region to provide durability and may contact with the lateral wall either to give additional anchoring of the stereocilia or to provide a route for interactions between the bundle and the lateral wall. [ABSTRACT FROM AUTHOR]
- Published
- 2008
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22. Comparative Distribution of Glutamate Transporters and Receptors in Relation to Afferent Innervation Density in the Mammalian Cochlea.
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Furness, David N. and Lawton, D. Maxwell
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HAIR cells , *MECHANORECEPTORS , *GLUTAMATE decarboxylase , *CELLS , *COCHLEA , *CORTI'S organ - Abstract
The local expression of proteins involved in handling glutamate may be regulated by the number and activity of synapses in regions of glutamatergic innervation. The systematically varying innervation of inner hair cells (IHCs) of the cochlea provides a model to test this suggestion. IHCs are glutamatergic and form a single row along the cochlear spiral. Along this row the number of afferent fibers terminating on IHCs increases toward the base, reaching a peak and thereafter declining. The afferents are segregated so that higher spontaneous rate fibers terminate on the pillar-cell side of the IHC and lower rate fibers terminate on the modiolar side. Using immuno-fluorescence and postembedding immunogold labeling, we investigated the distributions of the glutamate-aspartate transporter (GLAST or excitatory amino acid transporter 1), vesicular glutamate transporter (VGLUT1), and the AMPA receptor glutamate receptor 4 (GluR4) along the spiral. Immunofluorescent labeling for GLAST in IHC supporting cells increased in intensity to a peak in the region of 6-9 mm from the apex. Immunogold labeling for GLAST was greater overall in these cells in the 10 mm region than in the 1 mm region and also on the pillar-cell side of the IHC compared with the modiolar side. Immunogold labeling for GluR4 was confined to synaptic sites, represented by puncta in immunofluorescence. The relative numbers of puncta changed with a gradient similar to that of GLAST labeling. VGLUT1 labeling occurred in IHCs but showed no clear cochleotopic gradient. These data suggest that both the density of innervation and the activity levels of glutamatergic synapses may be involved in modulating regional expression of GLAST. [ABSTRACT FROM AUTHOR]
- Published
- 2003
- Full Text
- View/download PDF
23. Functional effects of a monoclonal antibody on mechanoelectrical transduction in outer hair cells
- Author
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Schulte, Claudia C., Meyer, Jens, Furness, David N., Hackney, Carole M., Kleyman, Thomas R., and Gummer, Anthony W.
- Subjects
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MONOCLONAL antibodies , *HAIR cells , *GENETIC transduction - Abstract
The functional effect of a monoclonal antibody, RA6.3, on mechanoelectrical transduction (MET) of outer hair cells (OHCs) isolated from the adult guinea-pig cochlea was investigated. This antibody was raised by an antiidiotypic approach against amiloride binding sites. RA6.3 irreversibly reduced the receptor current, independent of membrane potential. The time course of the functional block was independent of dilution (1:100, 50 and 10), beginning 1.2±0.5 min after the start of application and decreasing exponentially with a time constant of 0.29±0.18 min to 53±8% of the control current. The residual current was reversibly blocked by amiloride (300 μM), mainly at negative membrane potentials. Block of control current by amiloride was competitively inhibited by simultaneous application of RA6.3. These results suggest that RA6.3 binds to or in close proximity to amiloride receptor sites associated with the MET channels. Irreversibility, incompleteness, independence of membrane potential and independence of antibody dilution of the functional block can all be explained by irreversible binding of one antibody molecule to a receptor site, yielding a non-blocked state, followed by a relatively slow, reversible transition to a blocked state. It is proposed that the reversible transition might represent intramolecular binding of the second antibody combining site to the second receptor site. [ABSTRACT FROM AUTHOR]
- Published
- 2002
- Full Text
- View/download PDF
24. The role of transmembrane channel-like proteins in the operation of hair cell mechanotransducer channels.
- Author
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Kim, Kyunghee X., Beurg, Maryline, Hackney, Carole M., Furness, David N., Mahendrasingam, Shanthini, and Fettiplace, Robert
- Subjects
- *
HAIR cells , *MEMBRANE proteins , *MECHANOTRANSDUCTION (Cytology) , *STIMULUS & response (Biology) , *GENETIC mutation , *EXTRACELLULAR matrix - Abstract
Sound stimuli elicit movement of the stereocilia that make up the hair bundle of cochlear hair cells, putting tension on the tip links connecting the stereocilia and thereby opening mechanotransducer (MT) channels. Tmc1 and Tmc2, two members of the transmembrane channel-like family, are necessary for mechanotransduction. To assess their precise role, we recorded MT currents elicited by hair bundle deflections in mice with null mutations of Tmc1, Tmc2, or both. During the first postnatal week, we observed a normal MT current in hair cells lacking Tmc1 or Tmc2; however, in the absence of both isoforms, we recorded a large MT current that was phase-shifted 180°, being evoked by displacements of the hair bundle away from its tallest edge rather than toward it as in wild-type hair cells. The anomalous MT current in hair cells lacking Tmc1 and Tmc2 was blocked by FM1-43, dihydrostreptomycin, and extracellular Ca2+ at concentrations similar to those that blocked wild type. MT channels in the double knockouts carried Ca2+ with a lower permeability than wild-type or single mutants. The MT current in double knockouts persisted during exposure to submicromolar Ca2+, even though this treatment destroyed the tip links. We conclude that the Tmc isoforms do not themselves constitute the MT channel but are essential for targeting and interaction with the tip link. Changes in the MT conductance and Ca2+ permeability observed in the absence of Tmc1 mutants may stem from loss of interaction with protein partners in the transduction complex. [ABSTRACT FROM AUTHOR]
- Published
- 2013
- Full Text
- View/download PDF
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