1. Suppressive Effect of Tetrahydrocurcumin on Pseudomonas aeruginosa Lipopolysaccharide-Induced Inflammation by Suppressing JAK/STAT and Nrf2/HO-1 Pathways in Microglial Cells.
- Author
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Lin HW, Chen TC, Yeh JH, Tsou SC, Wang I, Shen TJ, Chuang CJ, and Chang YY
- Subjects
- Animals, Heme Oxygenase-1 metabolism, Janus Kinase 1, Lipopolysaccharides pharmacology, Mice, Microglia metabolism, NF-E2-Related Factor 2 metabolism, Nitric Oxide metabolism, Pseudomonas aeruginosa, Curcumin analogs & derivatives, Curcumin metabolism, Curcumin pharmacology, Inflammation chemically induced, Inflammation drug therapy, Inflammation metabolism
- Abstract
Brain inflammation, a pathological feature of neurodegenerative disorders, exhibits elevated microglial activity and increased levels of inflammatory factors. The present study was aimed at assessing the anti-inflammatory response of tetrahydrocurcumin (THC), the primary hydrogenated metabolite of curcumin, which was applied to treat Pseudomonas aeruginosa ( P.a. ) lipopolysaccharide- (LPS-) stimulated BV2 microglial cells. THC reduced P.a. LPS-induced mortality and the production of inflammatory mediators IL-6, TNF- α , MIP-2, IP-10, and nitrite. A further investigation revealed that THC decreased these inflammatory cytokines synergistically with JAK/STAT signaling inhibitors. THC also increased Nrf2/HO-1 signaling transduction which inhibits iNOS/COX-2/pNF κ B cascades. Additionally, the presence of the HO-1 inhibitor Snpp increased the levels of IP-10, IL-6, and nitrite while THC treatment reduced those inflammatory factors in P.a. LPS-stimulated BV2 cells. In summary, we demonstrated that THC exhibits anti-inflammatory activities in P.a. LPS-induced inflammation in brain microglial cells by inhibiting STAT1/3-dependent NF- κ B activation and inducing Nrf2-mediated HO-1 expression., Competing Interests: The authors reported no proprietary or commercial interest in any product mentioned or concept discussed in this article., (Copyright © 2022 Hui-Wen Lin et al.)
- Published
- 2022
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