1. Assessment of Short- and Medium-Chain Fatty Acids on Mitochondrial Function in Severe Inflammation.
- Author
-
Hecker M, Sommer N, and Mayer K
- Subjects
- DNA, Mitochondrial genetics, Fatty Acids chemistry, Fatty Acids, Volatile chemistry, Fatty Acids, Volatile pharmacology, Human Umbilical Vein Endothelial Cells, Humans, Inflammation pathology, Mitochondria pathology, Monocytes drug effects, Monocytes metabolism, Monocytes pathology, Tumor Necrosis Factor-alpha pharmacology, Fatty Acids pharmacology, Inflammation metabolism, Mitochondria drug effects, Mitochondria metabolism
- Abstract
Mitochondrial dysfunction is regarded as a key factor involved in the pathogenesis of septic disorders, leading to a decline in energy supply. The influence of short- and medium-chain fatty acids (SCFA/MCFA) on mitochondrial respiration under inflammatory conditions has thus far not been investigated. In the following protocol we describe the assessment of mitochondrial respiration using high-resolution respirometry under inflammatory and baseline conditions. For this approach, human endothelial cells and monocytes were pretreated with TNF-α to mimic inflammation followed by incubation with SCFA/MCFA and then subjected to high-resolution respirometry. Mitochondrial DNA content was assessed by PCR .
- Published
- 2021
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