1. New ionic liquids based on 5-fluorouracil: Tuning of BSA binding and cytotoxicity.
- Author
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Khachatrian, Artashes A., Mukhametzyanov, Timur A., Salikhov, Ramazan Z., Klimova, Alexandra E., Gafurov, Zufar N., Kantyukov, Artyom O., Yakhvarov, Dmitry G., Garifullin, Bulat F., Mironova, Diana A., Voloshina, Alexandra D., and Solomonov, Boris N.
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CYTOTOXINS , *IONIC liquids , *FLUOROURACIL , *FLUORESCENCE spectroscopy , *TERTIARY structure , *CHOLINE chloride , *IRINOTECAN - Abstract
In this work, we describe the synthesis, interactions with bovine serum albumin, and cytotoxicity of new ionic liquids based on 5-fluorouracil (API-ILs) with different cations (imidazolium, choline, isoquinolinium, guanidinium). The secondary and tertiary structure of BSA in solutions with different concentrations of API-ILs was monitored by the circular dichroism (CD) technique. The addition of API-ILs does not lead to structural changes in BSA. A quenching of fluorescence spectra intensity of BSA in presence of all API-ILs was observed, allowing the quantification of binding between API-ILs and BSA. The preferred localization of both ions in API-ILs differs significantly depending on the structure of the cation according to molecular docking. The aggregation of BSA in presence of API-ILs was analyzed by the dynamic light scattering (DLS) method, revealing a moderate increase in particle size. Cytotoxicity and selectivity of API-ILs on cancer and normal cell lines were estimated, showing a clear modification of the pharmaceutic activity of ionic liquid compared to 5-fluorouracil. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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