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Mung bean (Vigna radiata L.), a widely cultivated legume, belongs to the Fabaceae family's Papilionoideae subfamily. Although Phosphatidylethanolamine-binding protein (PEBP) genes have been identified in several plant species, their presence and function in mung bean remain largely unexplored. In this study, we identified seven VrPEBP genes from mung bean and classified them into four clades: FT, MFT, TFL and FT-like. Cis-regulatory element analysis revealed that VrPEBP genes may play a role in light, hormone, and stress responses. Quantitative real-time PCR (qRT-PCR) analysis indicated that VrPEBPs were constitutively expressed in various tissues. However, tissue-specific expression patterns were observed among VrPEBP genes. Under short-day (SD) conditions, VrFT1 and VrMFT1 exhibited significantly higher expression levels than under long-day (LD) conditions at 8 and 4 h, respectively. Conversely, VrTFL2 and VrTFL3 showed significantly higher expression levels under LD conditions compared to SD conditions at 8 and 12 h, respectively. The varied expression patterns of these genes under different photoperiod suggest their potential involvement in the photoperiodic regulation of flowering in mung bean. Additionally, phenotypic analysis of transgenic Arabidopsis plants overexpressing VrFT1 revealed higher expression levels under SD conditions and predicted its role in promoting flowering. These results provide valuable insights into the evolution and function of PEBP genes in mung bean and lay the foundation for further research on their regulatory mechanisms and potential applications in mung bean improvement. [ABSTRACT FROM AUTHOR]

Black gram and green gram are important pulse crops in India, but their production has faced fluctuations and stagnancy in yields over the last few decades. The Government of India has implemented several measures to enhance crop yield, including recommending and promoting the adoption of crop‐specific agronomic practices. However, there is limited empirical evidence on the determinants of the adoption of these practices and their impact on yield and income. In this context, this study analyses the determinants of the adoption of climate and plant management practices among black gram and green gram farmers and their impact on yield, crop revenue and net income across four major crop‐producing Indian states using a multinomial endogenous treatment effects model. Our analysis shows that information, contact with government extension services and access to off‐farm activities are crucial in adopting climate and plant management practices. The results strengthen the view that the adoption of knowledge‐intensive practices happens via formal information sources and plot‐level demonstrations. In addition, the results indicate that farmers who experience frequent crop loss exhibit an aversion towards adopting climate and plant management practices. While adopting these practices had a positive impact on crop yield and crop revenue, the impact on net income was observed only in the case of climate management. [ABSTRACT FROM AUTHOR]

The biological and organic fertilizers application under drought stress can reduce the adverse effects of this global issue and improve plant growth and development parameters. Therefore, this study was conducted in two crop years of 2020-2021 and 2021-2022 in the climatic conditions of Chalus city, Iran. The split-factorial experiment was conducted in the form of a basic randomized complete block design with 3 replications. The main factor included irrigation deficit at three levels (100, 80, and 60% of water requirement) and the secondary factors included biological fertilizer (control, mycorrhizal fungus, and Enterobacter) and organic fertilizer (no use, 16, and 24 tons per hectare) in a factorial manner. The results of the mean comparison showed that the highest seed yield was observed in the second year under 100% irrigation and application of 24 t/h of organic fertilizer and mycorrhiza at the rate of 126.1 g/m2; Subsequently, theco application of 80% water requirement and 24 t/h of organic fertilizer and Enterobacter treatments in the second year caused the highest amount of 124.3 g/m2. Generally, the 60% water requirement reduced grain yield compared to other water requirements levels. According to the results of this study, the organic and biological fertilization under drought stress conditions can significantly improve the quantitative and qualitative yield of mung bean. Therefore, it is recommended that the organic and biological fertilizers can be applied in drylands to mitigate drought stress conditions, and improve the growth, yield and quality of mung beans. [ABSTRACT FROM AUTHOR]

Inclusion of legumes in cereal based cropping system restores soil fertility and improves carbon storage and soil quality. In this background a field experiment was conducted in a strip-split plot design using three legume species viz., green gram, groundnut and cluster bean as vertical strip, two residue management practices i.e. residue removal (-R) and residue retention (+R) as horizontal strip, and three nitrogen levels i.e. 100%, 75% and 50% recommended rate of nitrogen (N) application in intersectional sub plots with four replications during summer 2017 and 2018. The grain and stover yield of maize, N uptake and soil properties were monitored after each crop harvest; soil nitrogen balance and soil organic carbon were estimated. Under 100% N fertilization, cluster bean – maize cropping system with residue (+R) recorded 12.78 and 9.78% higher maize grain and stover yield, respectively than without residue (–R) treatment. Highest available N (189.9 kg ha−1) and available P (54.5 kg ha−1) were registered under cluster bean- maize cropping system. Legume residue incorporation resulted 9.65, 8.46, 7.09 and 8.86% higher (p = 0.05) WBC content, WBC stock, available N and P contents over residue removal scenario. Annually, 1.71% added carbon converted into soil organic carbon. Legume residue retention and 100% recommended rate of nitrogen application enhanced nitrogen balance. Soil N balance was highest under groundnut – maize system (165 kg ha−1). Overall, for better soil health and optimum crop production, cluster bean – maize cropping system with legume residue retention and 100% N fertilization may be promoted in semi-arid Western India. [ABSTRACT FROM AUTHOR]

Background: Incidence of fall armyworm in maize has been reported at a severe level since 2018 resulting in low yield and in extreme cases complete failure of the crop. In view of undesirable effects of unilateral reliance on chemicals, greener avenues like intercropping needs to be explored, which promise to subside pests, as well as, provide higher income to cultivators. Methods: Field experiment was conducted during 2019-2021, consecutively for three years to investigate the consequences of intercropping with legumes viz., red gram (Cajanus cajan L.), black gram (Vigna mungo L.), green gram (Vigna radiata L. ), cowpea (Vigna unguiuculata L.), groundnut (Arachis hypogaea L.) and soybean (Glycine max L.) on incidence of fall armyworm in maize and the cost economics involved. Result: Among the different intercrops studied, maize+cowpea recorded significantly lowest mean per cent infested plants (45.0 per cent) at 75 days after sowing which was statistically on par with maize+green gram (45.5 per cent). The next effective intercropping system was maize+black gram (55.7 per cent) followed by maize+red gram (58.2 per cent). Maize as a sole crop recorded the highest mean per cent infested plants (76.3 per cent) at 75 days after sowing. The system equivalent yield (SEY) of the intercropping systems under study ascertained that the maize+cowpea achieved highest SEY of 5230 kg per ha followed by maize+green gram (5160 kg per ha) followed by maize+black gram (4900 kg per ha). Upon comparison of the benefit cost ratio (BC ratio), the maize+cowpea proved to be highly profitable with BC ratio of 4.11 followed by maize+green gram (4.05) followed by maize+black gram (3.85), whereas, sole maize recorded minimum BC ratio of 2.87. [ABSTRACT FROM AUTHOR]

This research aims to measure the nitrogen balance index (NBI) used in the evaluation of nitrogen (N) and chlorophyll (CHL), anthocyanin (ANTS), and flavonol (FLAV) contents of some cereal and legume microgreens. The experiment was established in the Fully Controlled Climate Room of Van Yuzuncu Yil University Faculty of Agriculture Department of Field Crops in OctoberNovember 2020. The plant materials used in the research were Bezostaja-1, Dogu-88, Palandoken and Ayyildiz wheat, Tarm-92, Cetin-2000, Larende and Kral-97 barley, Haskara, Dirilis, Kirklar, Kahraman and Faikbey oats, Arifiye maize, Uzbek and Sazak lentils. Arda chickpea, Goynuk bean, Mung bean, black chickpea, Amazon cowpea, Lutfibey sainfoin, Bilensoy-80 alfalfa, and Dadas red clover including 14 cereal and 10 legume varieties. The seeds planted in the planting medium consisting of the mixture prepared in plastic chalets with a 1331olüme of 500 cc were taken to a fully controlled climate room and microgreens were obtained by applying 50-60% humidity, 21 ± 2/17 ± 2 °C and 16/8 light/dark period, and microgreens were obtained in cereals and legumes. Polyphenolic measurements were taken when the microgreens were 7-9 days old and when sprouts of the forage legumes group were 20-21 days old. Measurements were performed in triplicate with the Dualex+ 4 Scientific (FORCE-A, Orsay, France) device. Statistical analysis of the measurements was carried out according to the Randomized Parcels Trial Design. According to the measurement results, the highest contents based on varieties were determined in Lutfibey sainfoin for NBI (106.533±10.68 mg/g), for CHL in Arda chickpea (41.3 ±2.63 µg/cm²), for ANTS in Dadas red clover (0.08 ±0.01 dualex index) and FLAV in Amazon cowpea (0.746 ±0.03 dualex index). According to group-based averages, the highest values were determined for NBI in forage legumes (86.844 mg/g), for CHL in barley (31.14 µg/cm²) and oats, edible cereal legumes and forage legumes in the same group, for ANTS in forage legumes (0.053 dualex index) and wheat and oats in the same group, for FLAV in edible cereal legumes (0.56 dualex index) and oat in the same group. The study concluded that cereal and legume microgreens have polyphenolic-rich contents. Similar research to be carried out under controlled and field conditions would be useful in evaluating bioactive ingredients and N. [ABSTRACT FROM AUTHOR]

Mung bean (Vigna radiata L.), a vital legume in Asia with significant nutritional benefits, is highly susceptible to Cercospora leaf spot (CLS) caused by Cercospora canescens, leading to significant yield losses. As an alternative to chemical fungicides, bio-priming with rhizobacteria can enhance plant resistance. This study explores the potential of Bradyrhizobium sp. strain DOA9 to augment resistance in mung bean against CLS via root priming. The results reveal that short (3 days) and double (17 and 3 days) priming with DOA9 before fungal infection considerably reduces lesion size on infected leaves by activating defense-related genes, including Pti1, Pti6, EDS1, NDR1, PR-1, PR-2, Prx, and CHS, or by suppressing the inhibition of PR-5 and enhancing peroxidase (POD) activity in leaves. Interestingly, the Type 3 secretion system (T3SS) of DOA9 may play a role in establishing resistance in V. radiata CN72. These findings suggest that DOA9 primes V. radiata CN72′s defense mechanisms, offering an effective bio-priming strategy to alleviate CLS. Hence, our insights propose the potential use of DOA9 as a bio-priming agent to manage CLS in V. radiata CN72, providing a sustainable alternative to chemical fungicide applications. [ABSTRACT FROM AUTHOR]

Vigna mungo. L (Black gram) belonging to the Leguminosae family is a most abundant source of protein for human and animal diets. Over 8.5 million tonnes of black gram are being produced worldwide, and after confiscating the endosperm for the protein source, the seed coat is dumped as agro-waste and used as feed for the cattle. This study aims to investigate and profile the metabolites in three distinct parts of V. mungo seeds: coat, cotyledon, and intact seed. Also, it has been hypothesized that sugar methylation plays a pivotal role in facilitating black gram seed germination by serving as an energy source during metabolic processes. In our study, A total of forty-nine compounds belonging to the class such as fatty acids, saccharides, triterpenes, sterols, and vitamins were detected in various parts of V. mungo seed by gas chromatography-mass spectrometry (GC-MS). Most interestingly, 3-O-methyl-D-glucose (3-OMG) was commonly detected in methanolic extract of coat, intact seed, and cotyledon with peak area percentages of 3.076, 1.157, and 1.100 %, respectively. Based on the highest peak area percentage in the coat, this compound was purified by using a silica gel column with a mesh size of 60–120 followed by Biotage SNAP cartridge KP-C18-HS (12 g). The yield and purity of the purified fractions were found to be 11.78 & 94.19 %, respectively by high-performance liquid chromatography (HPLC) analysis. Further, the mass confirmation of purified fraction containing 3-OMG (m/z - 217.06 g/mol) was done by liquid chromatography-mass spectrometry (LC-MS/MS) and reconfirmed with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/MS). The MS/MS analysis of the fragment showed m/z 206.91 for 3-OMG which corresponds to the association of the sodium adduct. The concentration of 3-OMG was quantified as 332.65 µg/g of dry weight in the coat of V. mungo by HPLC analysis. Also, among the antioxidant assays conducted, such as DPPH, ABTS, FRAP, and NOS, it was observed that the methanolic extract of coat exhibited the most significant antioxidant activity. The 3-OMG is commonly used as a non-metabolized analogue of glucose by regulating glucose transporter, especially GLUT-4 that regulates the absorption of glucose by insulin into muscle and fat cells. This study supports the black gram seed with coat as an alternative medicine to regulate blood glucose. [Display omitted] • The analysis focuses on the seed of Vigna mungo L which is produced globally. • Fatty acids, saccharides, triterpenes, sterols, and vitamins were detected by GC-MS in various parts of the V. mungo seed. • 3-O-methyl-D-glucose was detected in the coat with a high peak area percentage using GC-MS.3-O-methyl-D-glucose (3-OMG) detected in coat, seed, and cotyledon with high peak. • The MS/MS fragment of purified fraction for 3-OMG showed m/z 217.06 and was quantified at 332.65 μg/g of dry weight by HPLC. • Methanolic extract of the coat exhibited the highest antioxidant. [ABSTRACT FROM AUTHOR]

Vigna radiata (L.) R. Wilczek, generally known as mung bean, is a crucial pulse crop in Southeast Asia that is renowned for its high nutritional value. However, its cultivation faces substantial challenges due to numerous abiotic stresses. Here, we investigate the influence of salt and drought stress on mung bean genotypes by evaluating its morpho-physiological traits and biochemical characteristics. This phenotypic analysis revealed that both salt and drought stress adversely affected mung bean, which led to reduced plant height, leaf senescence, loss of plant biomass, and premature plant death. Reactive oxygen species (ROS) production increased under these abiotic stresses. In response, to prevent damage by ROS, the plant activates defense mechanisms to scavenge ROS by producing antioxidants. This response was validated through morpho-physiological, histological, and biochemical assays that characterized KVK Puri-3 and KVK Jharsuguda-1 as salt and drought sensitive genotypes, respectively, and Pusa ratna was identified as a drought and salt tolerant genotype. [ABSTRACT FROM AUTHOR]

Chickpea (Cicer arietinum L.) is an important legume crop worldwide. An emerging disease, basal stem rot with obvious wilt symptoms, was observed in the upper part of chickpea plants during the disease survey in Qiubei County of Yunnan Province. Three fungal isolates (ZD36-1, ZD36-2, and ZD36-3) were obtained from the diseased tissue of chickpea plants collected from the field. Those isolates were morphologically found to be similar to Diaporthe aspalathi. Molecular sequence analyses of multiple gene regions (ITS, tef1, tub2, cal, and his3) indicated that the three isolates showed a high identity with D. aspalathi. Pathogenicity and host range tests of the isolates were performed on the original host chickpea and eight other legume crops. The isolates were strongly pathogenic to chickpea and appeared highly pathogenic to soybean, cowpea, and mung bean; moderated or mild pathogenic to adzuki bean and common bean; however, the isolates did not cause symptoms on grass pea (Lathyrus sativus). Diaporthe aspalathi was previously reported as a main pathogen causing the southern stem canker in soybean. To our knowledge, this is the first report of D. aspalathi inducing basal stem rot on chickpea worldwide. [ABSTRACT FROM AUTHOR]

Mungbean (Vigna radiata) has gained increasing significance as a crucial grain legume in Nepal's tropical and sub-tropical regions due to its short growth cycle and soil fertility enhancement properties. This research was conducted at Agronomy farm of Lamjung campus, to assess how mungbean performs under varying row spacing and Npk fertilizer levels. The study employed two-factorial Randomized Complete Block Design with three row spacing levels (20 cm, 25 cm, and 30 cm) and three NPK fertilizer doses (10:10:10, 20:20:20, and 30:30:30 kg NPK/ha) as treatments. The investigation explored phenological and yield attributing parameters. Interestingly, the outcomes showed that row spacing had negligible impact on root growth attributes, including root length and nodule formation. However, root nodule number increased with higher NPK fertilizer levels, particularly notable with the application of (30:30:30) kg NPK/ha, resulting in the highest nodules per plant (140.40), while the lowest nodules (91.35) were observed with (10:10:10) kg NPK/ha. Vital traits like plant height, branch and leaf counts, pod set, pod length, and seed weight were unresponsive to both NPK doses and row spacing levels. In contrast, grain yield exhibited a substantial increase with NPK fertilizer application, with the highest yield (1263.55 kg/ha) achieved with (30:30:30) kg NPK/ha and the lowest (929.27 kg/ha) with (10:10:10) kg NPK/ha. Therefore, this study shows strong correlations, especially between root nodules and grain yield, emphasized key links. NPK doses impacted vital root nodules for nitrogen fixation and suggests dense planting of Pratikshya mung beans with increased NPK fertilizer. [ABSTRACT FROM AUTHOR]

The article presents a study on the management of Bihar hairy caterpillar in mungbean (Virgna radiata), the third most important pulse crop of India after chickpea and pigeonpea. Topics discussed include effect of various insecticidal treatments on Bihar caterpillar population in mungbean, yield of green gram under different treatments, and economics and cost benefit ratio treatments.

Simple Summary: In the process of symbiosis, ΔcopG1 in the type IV secretion system (T4SS) demonstrated the ability to invade root cells but was unable to survive and multiply within root cells. Conversely, traG1 and virD21 were found to be essential in the early stages of nodule formation. Intriguingly, copG1 is required for nod gene expression and acts as a repressor of T4SS genes. Moreover, the absence of copG1 results in certain proteins not being produced, especially T3SS (nopX and nopP) and C4-dicarboxylic acid (dct), which affects the symbiosis between Bradyrhizobium sp. SUTN9-2 and legumes. These findings support the hypothesis that the copG1 gene may serve as a new regulator of the symbiotic process. The symbiotic interaction between leguminous and Bradyrhizobium sp. SUTN9-2 mainly relies on the nodulation process through Nod factors (NFs), while the type IV secretion system (T4SS) acts as an alternative pathway in this symbiosis. Two copies of T4SS (T4SS1 and T4SS2) are located on the chromosome of SUTN9-2. ΔT4SS1 reduces both nodule number and nitrogenase activity in all SUTN9-2 nodulating legumes. The functions of three selected genes (copG1, traG1, and virD21) within the region of T4SS1 were examined. We generated deleted mutants and tested them in Vigna radiata cv. SUT4. ΔtraG1 and ΔvirD21 exhibited lower invasion efficiency at the early stages of root infection but could be recently restored. In contrast, ΔcopG1 completely hindered nodule organogenesis and nitrogenase activity in all tested legumes. ΔcopG1 showed low expression of the nodulation gene and ttsI but exhibited high expression levels of the T4SS genes, traG1 and trbE1. The secreted proteins from ΔT4SS1 were down-regulated compared to the wild-type. Although ΔcopG1 secreted several proteins after flavonoid induction, T3SS (nopP and nopX) and the C4-dicarboxylate transporter (dct) were not detected. These results confirm the crucial role of the copG1 gene as a novel key regulator in the symbiotic relationship between SUTN9-2 and legumes. [ABSTRACT FROM AUTHOR]

Background: Maize-wheat is the predominant cropping system of dryland ecology of Jammu region, but due to their comparatively higher input requirements especially of nutrients and water under the fragile ecology of these dry lands an untenable threat has been posed to their factor productivities. Therefore, all cropping sequences that suit and sustain better on the natural resources of the dryland ecosystems for a longer period of time needs to be explored. Methods: The treatments consisted of two oilseeds i.e. mustard) and gobhi sarson and two pulse crops i.e. chickpea and field pea taken during rabi were followed by two oilseed i.e. soybean and sesame and two pulse crops i.e. green gram and black gram grown during kharif. The experiment was laid out in randomized block design with four replications. Result: Significantly higher chickpea equivalent yield of green gram was observed with field pea-green gram sequence (10.26 q/ha) which was at par with the chickpea - green gram and field pea - black gram system. The available nitrogen status was significantly influenced and recorded highest (166.82kg/ha) under field pea-green gram system. Further overall nutrient mining by this system was quite low as compared to other systems. [ABSTRACT FROM AUTHOR]

Global food production is seriously threatened by salinity stress, which is becoming more severe due to human activity. For the majority of people worldwide, mungbean is a significant pulse crop and a rich source of protein and calories and hence is rightly marked as "Poor man's meat" and "rich man's vegetables". Salt stress, on the other hand, negatively affects the productivity of the mungbean since it causes decreased germination, reduced growth and development, interrupted photosynthesis, hormonal imbalance and eventually decreased yields. Consequently, developing mitigation strategies to deal with salt stress requires a greater understanding of how plants respond to salinity stress. The present review highlights the detrimental effects of salt stress and the physiological and biochemical tolerance mechanisms in mungbean. [ABSTRACT FROM AUTHOR]

The concept of plant-based protein consumption has been increasing recently because of the growing health consciousness among people. Mung bean is one of the most consumed legumes with a dense nutrient profile. Hence, current research is aimed to study the effect of mung bean protein-based products including mung bean snack (MBS) and textured vegetable protein (TVP) for treatment groups against the control groups, commercial ingredients group consisting of mung bean powder (MBP) and pea powder (PP) and commercial products group include commercial pea texture (cPT) and commercial textured vegetable protein (cTVP) for their proximate composition, digestibility, gut microbial profile and fatty acid metabolite profiling. The MBS and TVP samples had significantly higher digestibility of 74.43% and 73.24% than the commercial products. The protein content of TVP was 0.8 times higher than its commercial control. Gut microbiome profiling showed that all the samples shared around 162 similar genera. Post-fermentation analysis provided promising results by reflecting the growth of beneficial bacteria (Parabacteroides, Bifidobacterium and Lactobacillus) and the suppression of pathogens (Escherichia-Shigella, Dorea and Klebsiella). The dual relationship between gut microbiota and nutrient interaction proved the production of abundant short- and branched-chain fatty acids. The MBS sample was able to produce SCFAs (41.27 mM) significantly and BCFAs (2.02 mM) than the TVP sample (27.58 mM and 2.14 mM, respectively). Hence, our research outcomes proved that the mung bean protein-based products might infer numerous health benefits to the host due to enriched probiotics in the gut and the production of their corresponding metabolites. [ABSTRACT FROM AUTHOR]

Abstract Background Mung bean (Vigna radiata (L.) Wilczek), is an important pulse crop in the global south. Early flowering and maturation are advantageous traits for adaptation to northern and southern latitudes. This study investigates the genetic basis of the Days-to-Flowering trait (DTF) in mung bean, combining genome-wide association studies (GWAS) in mung bean and comparisons with orthologous genes involved with control of DTF responses in soybean (Glycine max (L) Merr) and Arabidopsis (Arabidopsis thaliana). Results The most significant associations for DTF were on mung bean chromosomes 1, 2, and 4. Only the SNPs on chromosomes 1 and 4 were heavily investigated using downstream analysis. The chromosome 1 DTF association is tightly linked with a cluster of locally duplicated FERONIA (FER) receptor-like protein kinase genes, and the SNP occurs within one of the FERONIA genes. In Arabidopsis, an orthologous FERONIA gene (AT3G51550), has been reported to regulate the expression of the FLOWERING LOCUS C (FLC). For the chromosome 4 DTF locus, the strongest candidates are Vradi04g00002773 and Vradi04g00002778, orthologous to the Arabidopsis PhyA and PIF3 genes, encoding phytochrome A (a photoreceptor protein sensitive to red to far-red light) and phytochrome-interacting factor 3, respectively. The soybean PhyA orthologs include the classical loci E3 and E4 (genes GmPhyA3, Glyma.19G224200, and GmPhyA2, Glyma.20G090000). The mung bean PhyA ortholog has been previously reported as a candidate for DTF in studies conducted in South Korea. Conclusion The top two identified SNPs accounted for a significant proportion (~ 65%) of the phenotypic variability in mung bean DTF by the six significant SNPs (39.61%), with a broad-sense heritability of 0.93. The strong associations of DTF with genes that have orthologs with analogous functions in soybean and Arabidopsis provide strong circumstantial evidence that these genes are causal for this trait. The three reported loci and candidate genes provide useful targets for marker-assisted breeding in mung beans.

Legumes are renowned for their distinctive biological characteristic of forming symbiotic associations with soil bacteria, mostly belonging to the Rhizobiaceae familiy, leading to the establishment of symbiotic root nodules. Within these nodules, rhizobia play a pivotal role in converting atmospheric nitrogen into a plant-assimilable form. However, it has been discerned that root nodules of legumes are not exclusively inhabited by rhizobia; non-rhizobial endophytic bacteria also reside within them, yet their functions remain incompletely elucidated. This comprehensive review synthesizes available data, revealing that Bacillus and Pseudomonas are the most prevalent genera of nodule endophytic bacteria, succeeded by Paenibacillus, Enterobacter, Pantoea, Agrobacterium, and Microbacterium. To date, the bibliographic data available show that Glycine max followed by Vigna radiata, Phaseolus vulgaris and Lens culinaris are the main hosts for nodule endophytic bacteria. Clustering analysis consistently supports the prevalence of Bacillus and Pseudomonas as the most abundant nodule endophytic bacteria, alongside Paenibacillus, Agrobacterium, and Enterobacter. Although non-rhizobial populations within nodules do not induce nodule formation, their presence is associated with various plant growth-promoting properties (PGPs). These properties are known to mediate important mechanisms such as phytostimulation, biofertilization, biocontrol, and stress tolerance, emphasizing the multifaceted roles of nodule endophytes. Importantly, interactions between non-rhizobia and rhizobia within nodules may exert influence on their leguminous host plants. This is particularly shown by co-inoculation of legumes with both types of bacteria, in which synergistic effects on plant growth, yield, and nodulation are often measured. Moreover these effects are pronounced under both stress and non-stress conditions, surpassing the impact of single inoculations with rhizobia alone. [ABSTRACT FROM AUTHOR]

Background: Mungbean is an ancient pulse crop widely cultivated under different agro-ecological situations in India. It is the potential source of protein, essential minerals and vitamin-B (vitamin-C in sprouting grains). Pulses offer an alternative for diversification of agricultural system but poor yield renders them poor competitors of cereals, oilseeds and cash crops. So it is necessary to screen and identify phenotypically stable genotypes which could perform more or less uniformly under different conditions. The current study aimed to identify the high potential stable genotype and appropriate sowing time for mungbean cultivation in hot arid zone of Rajasthan. Methods: A field experiment was conducted with 35 mungbean genotypes over four environments created by four different dates of sowing in RBD with three replications at SKRAU, Bikaner during summer-2019 and Kharif-2019 using the model of Eberhart and Russell (1966). Result: Stability parameters revealed that genotype IC-39269 exhibited stable performance for number of seeds per pod, biological yield per plant and harvest index across the environments; whereas, IPM 02-3, MH 2-15 and RMG-344 exhibited stable performance for seed yield under favourable environment i.e. Kharif season and genotype IC 103059 for stressed environment i.e. summer season. [ABSTRACT FROM AUTHOR]

Background: The production of pulses recorded a negative growth rate due to stagnation in expansion of pulse growing area and very slow progress in the improvement of productivity of important pulses. Therefore, there is urgent need to increase the area under pulse crops by promoting them into new areas and seasons. Blackgram [Vigna mungo (L.) Hepper] is third most important pulse crop both in acreage and production in India and hence, identification of high yielding dual season (kharif and summer) stable blackgram genotypes is very crucial for horizontal expansion of crop, which in turn leading to increased production. The present study was thus envisaged to identify stable, high yielding dual season (kharif and summer) genotypes. Methods: Twenty-five blackgram genotypes were evaluated at three different locations during kharif and summer seasons to identify high yielding, stable and suitable for dual season. The data obtained from six environments was subjected to stability analysis as per Eberhart and Russell (1966). Result: Analysis of variance for stability revealed that variance due to genotypes, environment and environment + (genotype × environment) were highly significant for all the characters. The genotypes viz., TRCRU-136, BDU-20, BDU-18, AKU-15 and TRCRU-22 were found early maturing with wider adaptability to unfavourable environments. While, BDU-18 and LBG-465 were found stable in expression with high mean performance across the six environments for 100-seed weight. From the present study, six promising stable genotypes viz., BDU-17, TRCRU-22, BDU-18, TRCRU-339, TRCRU-18 and IC 436778 were identified for cultivation during kharif as well as summer seasons. [ABSTRACT FROM AUTHOR]

Background: Mungbean [Vigna radiata (L.) Wilczek], commonly recognized as green gram, is the most significant and advanced pulse crop grown in Rajasthan. Dry root rot is one of the important diseases of mungbean caused by M. phaseolina, which is responsible for 30 to 40 per cent disease incidence with huge yield loss during seedling to mature stages. Methods: The experiments were conducted for two consecutive years during kharif 2020 and 2021 for managing the disease through organic amendments, plant extracts and bio-agents viz., Trichoderma harzianum (10 g/kg seeds), garlic extract (15%), neem cake (0.5 t/ha) and mustard cake (0.5 t/ha) alone and in combinations through seed as well as soil applications. Result: Our investigations cleared that the maximum disease reduction and highest seed yield were reported by treating the seeds with T. harzianum @10 g/kg seed + soil application of mustard cake @ 0.5 t/ha followed by seed treatment with T. harzianum @ 10 g/kg seed + soil application of neem cake @ 0.5 t/ha. In view of consumer health, present findings may be helpful for growers to get extra benefits by producing organic mungbean. [ABSTRACT FROM AUTHOR]

Background: Green gram (Vigna radiata), the third most important pulse crop, is grown in tank-fed, command areas after the harvest of paddy. In such areas, legume trees like Delonix regia and Peltophorum pterocarpum are commonly found and tree parts often fell in watercourses and were flown to crop fields along with irrigation water. Hence, it is necessary to study whether any allelopathic effect is present in these parts of trees which may affect the growth and development of green gram grown in farmland crops. Methods: Collected different tree parts viz., bark, leaf and fresh flower, a matured pod of Delonix regia and Peltophorum pterocarpum, cleaned and soaked distilled water at weight/volume ratio of 1:10 for 24 hrs. Aqueous extracts were filtered and tested for inhibition of green gram. Pot culture experiments were conducted with 10 treatments and four replications. Germination (%), shoot length (cm), root length (cm), fresh weight (mg), dry weight (mg), seedling vigour index-I and vigour index-II in green gram were recorded and the magnitude of inhibition versus simulation in bioassay was compared through response index. Result: Among the plant parts, Delonix regia flower and Peltophorum pterocarpum matured pods suppressed germination (45.00 and 60.00%), shoot length (13.87 and 13.39 cm), root length (4.27 and 10.49 cm), seedling vigour index-I (624.15 and 803.40) and seedling vigour index-II (960.75 and 948.00) respectively in green gram. This might due to presence of inhibitory chemicals in flowers of Delonix regia and matured pods of Peltophorum pterocarpum compared to other plant parts of trees. [ABSTRACT FROM AUTHOR]

Background: Maize (Zea mays L.) is one of the important cereal crop and its demand is increasing due to its versatile use but the productivity in India is still low. The basic requirement for improving the crop productivity is soil fertility and it is highly related with soil organic matter. Due to unavailability of organic manure and their higher cost, cheaper the organic source available at farm should be explored to meet the requirement. Methods: A field experiment was conducted at College Agronomy Farm, B.A. College of Agriculture, AAU, Anand during summerkharif seasons of years 2017 and 2018 to examine the impact of legume crop residue and nitrogen levels on maize crop. Experiment comprised of three legume crops grown in summer season viz., green gram, groundnut and cluster bean, two residue management treatments viz., residue removal and incorporation and three nitrogen levels in kharif maize viz., 100%, 75% and 50% recommended dose of nitrogen. Result: Results indicated that cluster bean-maize sequence provided the highest leaf area and leaf area index at 60 days after sowing, chlorophyll content and dry matter production at 30 and 60 days after sowing and crop growth rate for of maize crop. Incorporation of legume residues also had significant effect on plant height, dry matter production and chlorophyll content; leaf area and leaf area index at 60 days after sowing and crop growth rate and absolute growth rate of maize crop. Application of 100% RDN beneficially affected all the growth parameter and indices. Overall, summer legume cropping with their residue incorporation and higher nitrogen levels favourably impacts on various growth parameters and growth indices of maize crop. [ABSTRACT FROM AUTHOR]

Background: Mung bean (Vigna radiata (L.) Wilczek), is an important pulse crop in the global south. Early flowering and maturation are advantageous traits for adaptation to northern and southern latitudes. This study investigates the genetic basis of the Days-to-Flowering trait (DTF) in mung bean, combining genome-wide association studies (GWAS) in mung bean and comparisons with orthologous genes involved with control of DTF responses in soybean (Glycine max (L) Merr) and Arabidopsis (Arabidopsis thaliana). Results: The most significant associations for DTF were on mung bean chromosomes 1, 2, and 4. Only the SNPs on chromosomes 1 and 4 were heavily investigated using downstream analysis. The chromosome 1 DTF association is tightly linked with a cluster of locally duplicated FERONIA (FER) receptor-like protein kinase genes, and the SNP occurs within one of the FERONIA genes. In Arabidopsis, an orthologous FERONIA gene (AT3G51550), has been reported to regulate the expression of the FLOWERING LOCUS C (FLC). For the chromosome 4 DTF locus, the strongest candidates are Vradi04g00002773 and Vradi04g00002778, orthologous to the Arabidopsis PhyA and PIF3 genes, encoding phytochrome A (a photoreceptor protein sensitive to red to far-red light) and phytochrome-interacting factor 3, respectively. The soybean PhyA orthologs include the classical loci E3 and E4 (genes GmPhyA3, Glyma.19G224200, and GmPhyA2, Glyma.20G090000). The mung bean PhyA ortholog has been previously reported as a candidate for DTF in studies conducted in South Korea. Conclusion: The top two identified SNPs accounted for a significant proportion (~ 65%) of the phenotypic variability in mung bean DTF by the six significant SNPs (39.61%), with a broad-sense heritability of 0.93. The strong associations of DTF with genes that have orthologs with analogous functions in soybean and Arabidopsis provide strong circumstantial evidence that these genes are causal for this trait. The three reported loci and candidate genes provide useful targets for marker-assisted breeding in mung beans. [ABSTRACT FROM AUTHOR]

Background: Greengram Vigna radiata (L.) Wilczek, is an important legume crop that serves as a low-cost source of protein. The bruchid (Callosobruchus spp.) is a serious storage pest affecting greengram and other pulse crops. Thus, a study was designed to investigate bruchid resistance (Callosobruchus chinensis) in inter sub-specific derived lines of greengram and to identify chemical compounds responsible for resistance. Methods: The experimental material comprised of 200 inter sub-specific derived lines of F9 generation of VBN (Gg) 2 (susceptible to bruchid) x Vigna radiata var. sublobata/2 (resistant to bruchid) and a susceptible check variety. The bruchid screening experiment was carried out in completely randomized design and replicated twice with 50 seeds in each replication by adopting no choice test. Out of 200 lines evaluated for bruchid screening, seed damage due to bruchid was less than 20 per cent in 11 lines, identified as resistant. However, three resistant lines viz., GGISC 124, GGISC 140 and GGISC 150 were taken for further confirmation for bruchid resistance and GC-MS analysis to discover the chemical compounds conferring resistance (Clarus SQ 8C, Perkin Elmer). Result: In confirmation screening, seed damage due to bruchid (Callosobruchus chinensis) on 30th day was less than 20 per cent in three inter sub-specific lines viz., GGISC 124, GGISC 150 (17.00%) and GGISC 140 (18.00%), whereas the susceptible check [VBN (Gg) 2] reached 100 per cent adult emergence. The three inter sub-specific lines recorded susceptibility index of 0.046 (GGISC 124), 0.047 (GGISC 140) and 0.048 (GGISC 150) and classified as resistant. The susceptible check [VBN (Gg) 2] recorded the susceptibility index of 0.085. GC-MS study was carried out in resistant lines GGISC 124, GGISC 140, GGISC 150 and susceptible check VBN (Gg) 2. The results revealed that the existence of three compounds viz., 9, 12-octadecadienoic acid, methyl ester; Hexadecanoic acid, 1- (hydroxymethyl)-1, 2-ethanediyl ester and Hexadecanoic acid, 2-oxiranyl methyl ester in resistant lines conferred resistance against C. chinensis in greengram. [ABSTRACT FROM AUTHOR]

Black gram (Vigna mungo (L.) Hepper) is a pulses crop with good digestible protein and a high carbohydrate content, so it is widely consumed as human food and animal feed. Trichomes are large, specialized epidermal cells that confer advantages on plants under biotic and abiotic stresses. Genes regulating the development of trichomes are well characterized in Arabidopsis and tomato. However, little is known about trichome development in black gram. In this study, a high-density map with 5734 bin markers using an F2 population derived from a trichome-bearing and a glabrous cultivar of black gram was constructed, and a major quantitative trait locus (QTL) related to trichomes was identified. Six candidate genes were located in the mapped interval region. Fourteen single-nucleotide polymorphisms (SNPs) or insertion/deletions (indels) were associated with those genes. One indel was located in the coding region of the gene designated as Scaffold_9372_HRSCAF_11447.164. Real-time quantitative PCR (qPCR) analysis demonstrated that only one candidate gene, Scaffold_9372_HRSCAF_11447.166, was differentially expressed in the stem between the two parental lines. These two candidate genes encoded the RNA polymerase-associated protein Rtf1 and Bromodomain adjacent to zinc finger domain protein 1A (BAZ1A). These results provide insights into the regulation of trichome development in black gram. The candidate genes may be useful for creating transgenic plants with improved stress resistance and for developing molecular markers for trichome selection in black gram breeding programs. [ABSTRACT FROM AUTHOR]

Cadmium (Cd) toxicity is an alarming issue for our agricultural soils and serious threat to crop productivity. The concentration of Cd in our soils is continuously increasing which is posing serious threat to plants, animals and humans. Mung bean is a conventional pulse crop cultivated all over the world. Thus, this study's goal was to evaluate response of mung bean seedlings in terms of growth, physiology, and biochemistry to varying degrees of Cd stress. The investigation examined various Cd levels, including control, 5, 10 and 15 mg Cd/kg of soil. The results indicate that mungbean growth, physiological and biochemical components was negatively impacted by Cd stress. Results depicted that Cd (15 mg/kg) reduced the growth attributes photosynthetic pigments (Chl. a, b and carotenoids), total soluble proteins (TSP) and free amino acids (FAA) and increased the malondialdehyde (MDA), hydrogen peroxide (H2O2), and electrolyte leakage (EL). Interestingly, activities of all four antioxidants (ascorbic acid, catalase, ascorbate peroxidase and peroxidase) increased with increase in Cd toxicity. [ABSTRACT FROM AUTHOR]

Entada rheedii seeds are a rich source of protein (23.99% ± 0.07), starch (42.04% ± 0.05) and potassium (1670.15 ppm ± 116.732). HPTLC–UV analysis (seeds) reveals galactose in considerable amount, that is, 2.60% ± 0.008. Additionally, the species is low in fat and anti-nutrient metabolites like tannin. Interestingly, the proximates in Entada seeds was found comparable with the commonly consumed legumes like cowpea, green gram, and so on. The species exhibits promising anti-radical, anti-inflammatory and anti-diabetic activity. Data advocates the folklore use of E. rheedii seeds and can be a promising alternative source of dietary nutrition, fortified with medicinal value. Standardization and validation of scientific knowledge will bring such underutilized, low-cost legumes into daily dietary intake and are a promising source for Protein-Energy-Malnutrition. [ABSTRACT FROM AUTHOR]

Background: Pulses constitute the major source of dietary protein; therefore, it has ability to fulfil required protein recommended dietary allowance for vegetarian population of India. Pulse crop attack more than 200 insect pests and inflicting heavy damages at different growth stages that lead to significant economic losses to the farmers. The studies were conducted on green gram to find out the effective control measure of different crop stages against insect pests complex in green gram. Methods: The field experiment was laid out in a randomized block design and the consist 12 treatments with seed treatment and foliar sprays of insecticides at 50 per cent flowering stages then these treatments were replicated thrice at both years 2017 and 2018. Result: The experiments concluded that the reduction of sucking pests population like thrips, jassid and whitefly and larval population of Maruca vitrata and Helicoverpa armigera was found in seed treated with imidacloprid 48 FS and spray of flubendiamide at 50 per cent flowering stage in green gram with Rs. 10,678 net profit and 1:1.42 cost-benefit ratio. [ABSTRACT FROM AUTHOR]

Mung bean cropping plays a vital role in providing essential nutrition and serving as a significant summer crop to feed billions of people worldwide. However, allelopathy poses a threat to the sustainability and productivity of this system. The residues of pearl millet plants contain allelochemicals that can negatively impact weed growth in various crops. This study aimed to evaluate the potential allelopathic effects of pearl millet powder on the germination, growth, pigments, protein, and carbohydrate levels of mung beans. Different quantities of whole plant powder, namely 0, 5, 10, and 15 g of pearl millet, were blended into medium-sized pots, each containing 500 g of soil. Mung bean seeds were grown in these pots for 3–4 days in a greenhouse before transplanting them into an open field. The findings revealed that as the quantity of pearl millet powder increased, the germination percentage, germination speed, plant height, fresh weight, dry weight, chlorophyll content, carotenoid, protein, and carbohydrate amount of mung beans decreased. The physio-chemical properties of soils treated with pearl millet exhibited higher pH, conductivity, total solids, and heavy metal content than control soils. The decrease in the aforementioned attributes in soils treated with pearl millet powder could be due to the release of allelochemicals and/or the accumulation of heavy metals in the soil.These results demonstrate that using pearl millet as an herbicide to control weeds is effective, but caution must be exercised if heavy metals accumulate in the pearl millet tissue. [ABSTRACT FROM AUTHOR]

As the best-fit leguminous crop for intercropping across time and space, mungbean promises to sustain soil health, carbon sequestration, and nutritional security across the globe. However, it is susceptible to waterlogging, a significant constraint that persists during heavy rains. Since the predicted climate change scenario features fewer but more intense rainy days. Hence, waterlogging tolerance in mungbean has been one of the major breeding objectives. The present experiment aimed to employ nondestructive tools to phenotype stress tolerance traits in mungbean genotypes exposed to waterlogging and estimate the association among the traits. A total of 12 mungbean genotypes were used in the present study to assess waterlogging tolerance at the seedling stage. Plant responses to stress were determined non-destructively using normalized difference vegetation index (NDVI) and chlorophyll fluorescence parameters at different time intervals. NDVI and grain yield were positively associated with control (r = 0.64) and stress (r = 0.59). Similarly, chlorophyll fluorescence (quantum yield of PS-II) also had a significant positive association with grain yield under both control (rD 0.52) and stress (rD0.66) conditions. Hence, it is suggested that NDVI and chlorophyll fluorescence promise to serve as traits for non-destructive phenotyping waterlogging tolerance in mungbean genotypes. With the methods proposed in our study, it is possible to phenotype hundreds of plants for waterlogging tolerance efficiently. [ABSTRACT FROM AUTHOR]

Mung bean (Vigna radiata L.) is a major pulse crop in many parts of the world, but its yields are generally lower than other pulse crops due to biotic and abiotic stresses, including nutrition. An experiment was conducted to examine the effects of localized application of phosphorus (P) (32P labeled P fertilizer) and different sources of potassium (K) (KNO3, KCl, K2SO4 and KH2PO4) on shoot and root growth of mung bean, nutrient uptake and applied P and K recovery on a sandy loam Yellow Chromosol soil with low P and K status. Among the K sources highest shoot and root yield was obtained with K2SO4. Similarly, total plant P and the percentage of total P content obtained from the fertilizer (Pdff) was highest in this treatment. P application resulted in an increase in root length/soil mass, root mass density both inside and outside the band and in the percentage of roots in the fertilizer band. Each of the above parameters was numerically highest in the P+K2SO4 surface applied treatment. Root growth in the banded P resulted in better mung bean growth and nutrient uptake which was associated with stimulated root growth in the band and/or through increased uptake of P per unit root length. Co-application of K with P did not stimulate P uptake. Since co-application of P and K did not result in a synergistic response to either nutrient separation of these nutrients can be practiced. [ABSTRACT FROM AUTHOR]

Background: Symbiotic associations between legumes and Rhizobia are ancient and fundamental. However, the plant growthpromoting endophytes other than Rhizobia are not yet fully explored for pulses productivity. The present study was aimed to isolate efficient endophytic bacteria from pulses, assess their diversity, screen their plant growth-promoting activities and to test their potential as bio inoculants for pulses. Methods: We have isolated several endophytic bacteria from pulse crops more specifically from blackgram (Vigna mungo) and greengram (Vigna radiata). After careful screening, 15 promising endophytic isolates were selected for this study. The identification of endophytic bacterial isolates was performed by 16S rRNA gene sequencing. The isolates were tested for their potential for the plant growth-promoting traits such as nitrogen fixation, phosphate solubilization, indole-3-acetic acid production, siderophore secretion and antifungal activity. Pot culture experiments were conducted with the screened potential endophytic cultures. Result: The 16S rRNA gene sequencing revealed that species of Enterobacter, Bacillus, Pantoea, Pseudomonas, Acromobacter, Ocrobacterium were found as endophytes in blackgram and greengram. The in vitro screening identified Bacillus pumilus (BG-E6), Pseudomonas fluorescens (BG-E5) and Bacillus licheniformis (BG-E3) from blackgram and Pseudomonas chlororaphis (GG-E2) and Bacillus thuringiensis (GG-E7) from greengram as potential plant growth-promoting endophytes. These strains showed antagonism against plant pathogenic fungi. Upon inoculation of these endophytic PGPR strains, the blackgram and greengram growth and yield got increased. Among the strains, BG-E6 recorded 14.7% increased yield in blackgram and GG-E2 accounted for a 19.5% yield increase in greengram compared to respective uninoculated control. The experimental results showed that there was a host specificity found among the endophytic bacterial cultures with pulses. The cross inoculation of endophytic strains did not perform well to enhance the growth and yield of their alternate hosts. [ABSTRACT FROM AUTHOR]

Carbohydrates are significant components of legumes, and their profiling can provide information about their nutritional value and potential health benefits. This study explores the proximate composition and carbohydrate profiles of accessions of three underutilized legumes, namely, Vigna radiata, Vigna mungo, and Macrotyloma uniflorum. The total starch and soluble sugar are determined. Starch granule morphology and diameter are determined using scanning electron microscopy and chain length distribution of amylopectin is analyzed using Capillary Electrophoresis. All accessions of the legumes vary both in their proximate composition and carbohydrate components. Accessions of M. uniflorum (PI‐658594‐01‐SD) have the highest carbohydrate component (7.6%) while V. radiata (TVR‐42) has the least (4.2%). Macrotyloma uniflorum accensions (PI‐658594‐01‐SD, and PI‐180437‐01‐SD) have the highest starch and sucrose contents, while V. mungo accensions (TVM‐13, and TVM‐11) have the lowest. The glucose and fructose contents are lower than sucrose in all the accessions of the legumes. Macrotyloma uniflorum accensions exhibit larger granules while accessions of Vigna species have the smallest granules. All species accessions exhibit similar amylopectin chain length distribution profiles although accessions of V. mungo slightly differ in their proportion of long and short glucan chains. The three species of underutilized legumes exhibit unique characteristics which make them suitable for consumption and may be exploited as a source of nutraceuticals and pharmaceuticals. [ABSTRACT FROM AUTHOR]

The present investigation sought to evaluate the genetic variability and determine well-suited genotypes for crossbreeding initiatives with the goal of augmenting the desirable traits of Vigna radiata (L.) Wilczek, more commonly referred to as green gram. This crop holds significant significance as a legume grain in India. Ninety-four genotypes were evaluated for various quantitative traits, and statistical analysis was conducted to estimate genetic parameters and determine divergence. Significant variations were observed among the genotypes for all studied traits, indicating a wide genetic diversity. Phenotypic observations revealed the influence of both genotype and environment on the expression of traits. Biometric parameters, such as genotypic coefficient of variation, broad-sense heritability, and genetic advance, were used to measure the observed variability. The results provide valuable insights for breeders to develop selection programs for genetic improvement of green gram. Additionally, clustering analysis based on D2 distance categorized the genotypes into 12 distinct clusters, highlighting their unique genetic characteristics. The study identified traits contributing significantly to divergence, with the number of pods per plant showing the highest contribution. This research contributes to the development of superior green gram varieties through enhanced genetic understanding and selection strategies. [ABSTRACT FROM AUTHOR]

In tropical and subtropical countries of the world, Callosobruchus maculatus (F.) is a crucial pest of pulses. After the harvest, pulse beetles in storage cause qualitative and quantitative worsening of legumes. Survival of different geographical strains of cowpea weevil may vary on different host legumes. The research was conducted to examine screening for ovipositional preference, growth and development of cowpea weevil, on nine common legume hosts, viz., cowpea, green gram, pigeon pea, pea, desi chickpea, kabuli chickpea, black gram, soybean and red lentils. In the present study, life phases of Lucknow strain (variant) of C. maculatus, was seen on nine stored pulses at laboratory condition for the assessment of growth and development and better management in storage. The outcome of present studies, indicated that the cowpea weevil favour cowpea, green gram, pigeon pea, pea, for its highest oviposition because the highest number of eggs was found on these legumes followed by desi chickpea, kabuli chickpea while on black gram, soybean and red lentils lowest number of eggs were observed. The developmental time of cowpea weevil was short on cowpea, green gram, pigeon pea, pea, followed by desi chickpea, kabuli chickpea, while the longest developmental period was on the black gram, soybean and red lentils. The percent adult emergence, percent weight loss and growth index was highest in cowpea, green gram, pigeon pea and garden pea, desi chickpea, and kabuli chickpea whereas lowest percent of adult emergence, weight loss and growth index was found in black gram, soybean and lentil after three generations. From this research it was clear that cowpea, green gram, pigeon pea, pea, desi chickpea, kabuli chickpea was most preferred host legumes among all nine examined stored legumes, whereas black gram, soybean, and red lentils were least preferable hosts to the Lucknow strain of C. maculatus. [ABSTRACT FROM AUTHOR]

Being a pulse crop, green gram has huge scope in crop improvement in terms of productivity and other yield related parameters. Genetic variability is a preferable option for breeders in breeding programme for varietal development programme and others crop improvement aspect. The present investigation was framed to create the variability in mung bean var. GAM 8 during the seedling period through gamma (γ) irradiation. The significant variation was observed in germination percentage and it was found that dose dependent relationship between the germination rate and dose. Significantly minimum germination percentage (22.38 %) was observed in 700 Gy but optimal lethal dose (LD50) calculated through Probit analysis based on germination percentage was revealed at 540.26 Gy. Data revealed that γ irradiation had significantly reduced the seedling growth parameters such as shoot length (10.05 cm), shoot dry weight (19.68 mg), seedling length (13.90 cm), vigour index I (311.01) and II (509.01) was registered in 700 Gy while minimum root length (3.83 cm) was occurred in 600 Gy γ irradiation. Growth reduction (GR) 50 and 30 with respect to the seedling length was occurred respectively at 1093.79 and 1469.74 Gy. Therefore, this finding as the source of genetic variability would be used in future breeding and crop improvement programme like enhancement of yield potentiality and stress management in mung bean var. GAM 8. [ABSTRACT FROM AUTHOR]

Background: Pulses are having a number of constraints in their production as compared to their potential in India. Green gram is an important pulse crop of the country after chickpea and pigeonpea lacks optimum fertilizer management, which leads in reduced growth and yield. The current study aimed to study the effects of different levels of potassium and zinc application on growth, yield attributes, yield and quality of green gram. Methods: The field study was conducted during Kharif 2018 at Regional Research Station, CCS HAU, Bawal on green gram cultivar MH-421. The experiment was laid out in a split-plot design with three replications and treatments comprising four levels of potassium in main plots (0, 10, 20 and 30 kg K2O ha-1) and four levels of zinc in subplots (0, 12.5, 25 and 37.5 kg ZnSO4 ha-1). Result: The results indicated that growth parameters like plant height, number of branches, number of pods, number of seeds and dry matter production were released due to the application of potassium and zinc. The number of nodules, their fresh weight and quality parameters like protein content and seed index significantly increased with the application of potassium up to 20 kg K2O ha-1, but non-significant increment with zinc application. Seed and straw yield increased significantly with potassium and zinc application up to the levels of 20 kg K2O ha-1 and 25 kg ZnSO4 ha-1, respectively. Higher net monetary returns of Rs. 31528 ha-1 with B:C ratio (1.61) was recorded under the treatment combination K20Zn25 (20 kg K2O ha-1 along with 25 kg ZnSO4 ha-1). It was concluded that higher green gram yield with better quality and more net return was achieved when potassium and zinc were applied @ 20 kg K2O ha-1 and 25 kg ZnSO4 ha-1, respectively. [ABSTRACT FROM AUTHOR]

Background: Intercropping maize with legumes can enhance diversity, stability, complementary sharing of resources, maintain soil fertility, productivity and livelihood of the farmers. The current study was aimed to develop the scientific knowledge of maize and pulse intercropping and their combinations in different planting structures. Methods: The field experiment consisted of seven treatments viz. sole maize, sole soybean, sole green gram and two intercropping ratios of soybean and green gram with maize at 1:1 and 1:2 row ratios and laid out in randomized block design in four replications during 2018-19 at Imphal. The intercropping, economic and competitive efficiency indices were calculated accordingly. Result: Intercropping of maize either with soybean or green gram in 1:2 ratio gave the best combination based on intercropping and economic efficiencies. Maize + soybean at 1:2 row produced the highest maize equivalent yield (82.65 q ha-1), system productivity (22.60 kg ha-1 day-1) and production efficiency (55.84 kg ha-1 day-1). Maize + green gram at 1:2 ratio had significantly higher land equivalent ratio (1.7) and land equivalent co-efficient (0.54) whereas maize + soybean at 1:2 ratio produced the highest area time equivalent ratio (1.27). Competition between the crops was higher under maize + green gram at 1:1 ratio. [ABSTRACT FROM AUTHOR]

Background: Greengram is third most important pulse crop which is widely adapted and can improve soil fertility. The genetic variability and association studies help in selection which would increase the yield potential of greengram. Methods: Two hundred stabilized lines (F9) obtained from the cross VBN (Gg) 2 and Vigna radiata var. sublobata were evaluated. The yield and yield contributing traits were recorded to evaluate genetic variability, heritability, genetic advance as % of mean and correlation among the yield and its component traits. Result: Analysis of variance exhibited significant differences among the lines for all the ten quantitative traits indicating the presence of genetic variability. The high GCV values were found for the traits viz., single plant yield (30.18) and the number of pods per plant (26.80). High heritability combined with high genetic advance as per cent of mean was observed for the number of pods per plant (90.19, 52.43), single plant yield (89.58, 58.84), the number of pods per cluster (79.28, 22.70) and the number of clusters per plant (69.50, 30.22) which indicates additive gene effects. In the present study, the number of pods per plant had a high positive direct effect (0.919) on a single plant yield. Therefore, the selection for these characters would improve yield in greengram. [ABSTRACT FROM AUTHOR]

Cancer is still a major challenge for humans. In recent years, researchers have focused on plant‐based metabolites as a safe, efficient, alternative or combinatorial, as well as cost‐effective preventive strategy against carcinogenesis. Mung bean is an important nutritious legume, and known for providing various health benefits due to various bioactive phytochemicals and easily digestible proteins. Regular intake of mung bean helps to regulate metabolism by affecting the growth and survival of good microbes in the host gut. Mung bean has also been reported to have anti‐inflammatory, antioxidant, antiproliferative, and immunomodulatory properties. These properties may possess the preventive potential of mung bean against carcinogenesis. Bibliographic databases for peer‐reviewed research literature were searched through a structured conceptual approach using focused review questions on mung beans, anticancer, therapeutics, and functional foods along with inclusion/exclusion criteria. For the appraisal of the quality of retrieved articles, standard tools were employed. A deductive qualitative content analysis methodology further led us to analyze outcomes of the research and review articles. The present review provides recent updates on the anticancer potential of mung bean and the possible mechanism of action thereof to prevent carcinogenesis and metastasis. Extensive research on the active metabolites and mechanisms of action is required to establish the anticancer potential of mung bean. Keeping the above facts in view, mung bean should be investigated for its bioactive compounds, to be considered as functional food of the future. [ABSTRACT FROM AUTHOR]

The most widely used legume beans food in Ethiopia are fava beans (Vicia faba), green gram (Vigna radiata), white lupin (Lupinus albus), and three types of kidney beans (Phaseolus vulgaris). These legume crops are grown in various regions of Ethiopia and can absorb significant amounts of fluoride from air, water, and soil. This study examined the fluoride content of legume beans collected from four different regions of Ethiopia by ion selective electrode potentiometry. Fluoride concentrations in this study were found to range from 1.9 to 22.8 mg/kg. Fava bean sample from Asella was found to contain higher fluoride (22.8 mg/kg) than in the fava beans from other sites and other beans from any site. Fluoride levels (13.0 mg/kg) in kidney bean from Migira was in the middle of the range while the fluoride levels (2.5 mg/kg) in the white lupin and green gram (1.9 mg/kg) were found in the lower end of the range. This study indicates that daily consumption of 100 g of fava and kidney beans may results in health problem while that of green gram and white lupin are safe for human health (will not exceed 3 mg/day set by Food and Nutrition Board of USA). [ABSTRACT FROM AUTHOR]

Sustainable agriculture is a key factor to meet the global demand of food supply. In recent years, nanotechnology has been adopted as a promising tool for achieving the sustainable agriculture. The advent of nanotechnology may play a significant role from farm to fork, by improving the yield and quality. Several approaches are utilized viz nano-farming, nanoparticles-enabled smart delivery system, nanofertilizers, nanomaterials to enhance the quality by promoting the seed germination, increasing biochemical constituents, adaptation and resistance to diseases. Polyherbal nanoformulation (PHNF) was formulated using green nanotechnology with the assistance of triphala extract and silver nitrate solution. Synthesized PHNF was physico chemically confirmed for the formation of nanosized particles. This research focuses on applications of polyherbal nanoformulation (PHNF) on plant growth promotion in Vigna radiata and Trigonella foenum-graecum. Germinating seeds were sprayed with 1 ppm, 5 ppm, 10 ppm of PHNF and compared with the control. Upon PHNF treatment the growth parameters including shoot length, root length and fresh weight were increased in all two tested species. There was a gradual increase in the photosynthetic pigments including total chlorophyll, chlorophyll a, chlorophyll b, carotenoids and biochemical constituents including proteins and carbohydrates and is concentration dependent. These results suggested that polyherbal nanoformulation can be used as an alternative to chemical fertilizers to overcome the drawbacks of synthetic fertilizers and to provide eco-friendly solution for plants and protect the ecosystem from hazardous chemicals. [ABSTRACT FROM AUTHOR]

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Rising temperatures impact different developmental stages of summer crops like mung bean, particularly during the crucial seed-filling stage. This study focused on twomung bean genotypes, categorized as heat-tolerant [HT] or heat-sensitive [HS]. These genotypes were grown in pots in an outdoor natural environment (average day/night temperature 36°C/24.3°C) until the onset of podding (40 days after sowing) and subsequently relocated to controlled-environment walk-in growth chambers for exposure to heat stress (42°C/30°C) or control conditions (35°C/25°C) until maturity. For all measured attributes, heat stress had a more pronounced effect on the HS genotype than on the HT genotype. Heat-stressed plants exhibited severe leaf damage, including membrane damage, reduced chlorophyll content, diminished chlorophyll fluorescence, and decreased leaf water content. Heat stress impeded the seed-filling rate and duration, decreasing starch, protein, fat, and mineral contents, with a notable decline in storage proteins. Heat stress disrupted the activities of several seed enzymes, inhibiting starch and sucrose accumulation and consequently decreasing individual seed weights and seed weight plant-1. This study revealed that heat stress during seed filling severely impairedmung bean seed yield and nutritional quality due to its impact on various stress-related traits in leaves and enzyme activities in seeds. Moreover, this research identified potential mechanisms related to heat tolerance in genotypes with contrasting heat sensitivity. [ABSTRACT FROM AUTHOR]

Mungbean is an important pulse crop and it is mainly cultivated in Asia for human consumption. Its small genome and diploid nature make it a well-suited model organism among legume crops. Thus, cost-effective, reliable and highly polymorphic molecular markers distributing the whole genome are needed for diversity, mapping and functional genomics studies in this model species. The wholegenome sequence of mungbean was obtained and used as a source of identification of simple sequence repeats (SSR). The sequence reads were aligned and SSRs detection was performed using the Phobos plugin tandem repeat finder in the Geneious software program. A total of 12 mungbean genotypes were selected to validate the newly developed SSR markers. In the present study, a total of 12, 49,774 and 11, 86, 386 perfect and imperfect SSR repeats were identified from the mungbean genome. The tri-repeats were the most abundant (26.10%), followed by hexa (20.82%), penta (20.45%), tetra (17.65%) and di-repeats (14.95%). We designed 1330 SSR primers based on the genomic sequence of flanking perfect SSRs (Di and tri-repeats). Among them, 50 SSR primers uniformly distributed across the 11 mungbean chromosomes were selected and used to validate 12 mungbean genotypes. The newly developed genomic SSR markers generated in the present study are a valuable genomic resource for the mungbean breeding programs. [ABSTRACT FROM AUTHOR]

This current study is to examine the effects of inorganic fertilizers which are allegedly able to reduce the population of indigenous bacteria and the application of bacteria from VP3 biofertilizer on three test plants (legumes). In all treatments, the addition of inorganic fertilizer at a dose of 50% and 75% could significantly reduce population of soil bacteria on the 10th and 49th day observations. This also shows that the higher dose of NPK fertilizer also affects the bacteria from VP3 biofertilizer. However, treatment with 25-100% NPK fertilizers caused the decreasing of soil bacteria since the day of planting. In bean and long bean plants, the highest yields were shown at the combination of compost, VP3 biofertilizer and the addition of 75% NPK. Meanwhile, for mung bean, the highest yields were produced from the combination treatment of compost, VP3 and 50% and 75% NPK biofertilizers. However, the treatment of VP3 biological fertilizer with compost without the addition of NPK fertilizer on 3 legumes was able to give higher yields than the treatment of single NPK fertilizer. [ABSTRACT FROM AUTHOR]

Legume inclusion into cropping systems has been proposed to maintain high crop yields while offering multiple environmental benefits. However, the effect of legumes as pre-crop on subsequent wheat yield and soil has not been well explored. Thus, a 7-year field experiment was used to determine the interactive effects of mineral fertilization and legumes (peanut, mung bean, soybean, adzuki bean) inclusion on wheat productivity and soil quality. Our results showed that legume inclusion led to a higher wheat yield advantage (52% on average) than maize–wheat rotation under no fertilization but the advantage decreased to 26% with fertilization. All legume–wheat rotation systems supported stable wheat production, where a stronger effect was observed after peanut than after maize. Meanwhile, the wheat yield under legume–wheat systems was more resistant (i.e., less variability in the yield after ceasing fertilization) and more resilient (i.e., recovering more quickly after fertilizer re-application) relative to maize-wheat. Furthermore, soil ecosystem multifunctionality increased by 0.8 times in the topsoil while maintaining soil organic carbon stocks, even with low C and N inputs under legume–wheat. Interestingly, we also observed a positive correlation between wheat yield and soil ecosystem multifunctionality. In conclusion, legume inclusion as a sustainable practice can optimize crop yields by enhancing soil multifunctionality while maintaining soil organic carbon stocks, particularly for integration into low-yielding agroecosystems. [ABSTRACT FROM AUTHOR]