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Your search keyword '"E. Cesar B. Nadala"' showing total 3 results
Start Over Author "E. Cesar B. Nadala" Topic medicine
3 results on '"E. Cesar B. Nadala"'

1. Production of polyclonal antiserum specific to the 27.5 kDa envelope protein of white spot syndrome virus

Author

Zerong You, E. Cesar B. Nadala, Jinsheng Yang, Marielle C. W. van Hulten, and Philip C. Loh

Subjects
White spot syndrome, Immunoblotting, Laboratory of Virology, Aquaculture, Aquatic Science, medicine.disease_cause, Antibodies, Viral, Polymerase Chain Reaction, Virus, law.invention, Laboratorium voor Virologie, monodon, Viral envelope, Penaeidae, Viral Envelope Proteins, law, medicine, Escherichia coli, pathogenicity, Animals, Ecology, Evolution, Behavior and Systematics, Antiserum, biology, Immune Sera, DNA Viruses, biology.organism_classification, Fusion protein, Virology, Molecular biology, Specific Pathogen-Free Organisms, wsbv, Molecular Weight, Polyclonal antibodies, biology.protein, Recombinant DNA, Rabbits, baculo-like virus, penaeid shrimp
Abstract

A truncated version of the white spot syndrome virus (WSSV) 27.5 kDa envelope protein was expressed as a histidine tag fusion protein in Escherichia coli. The bacterial expression system allowed the production of up to 10 mg of purified recombinant protein per liter of bacterial culture. Antiserum from a rabbit immunized with the recombinant protein recognized the 27.5 kDa viral envelope protein of WSSV isolated from different geographic regions. The antiserum did not recognize any of the other known WSSV structural proteins. A sensitive immunodot assay for WSSV was developed using the specific rabbit polyclonal antiserum.

Published
2002

2. Dot-blot nitrocellulose enzyme immunoassays for the detection of white-spot virus and yellow-head virus of penaeid shrimp

Author

E. Cesar B. Nadala and Philip C. Loh

Subjects
Gills, animal structures, Penaeidae, viruses, Immunoblotting, Dot blot, Biology, Antibodies, Viral, Virus, Immunoenzyme Techniques, Virus antigen, Virology, Collodion, Decapoda, medicine, Yellow-head virus, Animals, Antigens, Viral, medicine.diagnostic_test, fungi, biology.organism_classification, Molecular biology, Shrimp, Immunoassay, Rabbits, Baculoviridae
Abstract

Dot-blot nitrocellulose enzyme immunoassays (DB-NC-EIA) were developed for the detection of white-spot virus (WSV) and yellow-head virus (YHV) in infected shrimp. The assays utilized HRP-conjugated virus-specific antibodies to detect virus antigen present in gill homogenates of infected shrimp spotted onto nitrocellulose membrane. The assays are by far the simplest and most rapid detection methods available for WSV and YHV.

Published
2000

3. A nitrocellulose-enzyme immunoassay method for the detection of hepatitis A virus

Author

E. Cesar B. Nadala and Philip C. Loh

Subjects
viruses, Guinea Pigs, Enzyme immunoassay method, Hepatitis A Antigens, Biology, Hepatitis A Antibodies, Sensitivity and Specificity, Virus, Immunoenzyme Techniques, chemistry.chemical_compound, Antigen, Virology, Collodion, medicine, Animals, Humans, Hepatitis Antibodies, Hepatovirus, Antigens, Viral, Binding Sites, medicine.diagnostic_test, fungi, virus diseases, biochemical phenomena, metabolism, and nutrition, Hepatitis A, Molecular biology, digestive system diseases, Hepatitis a virus, chemistry, Immunoassay, Immunoglobulin G, Nitrocellulose
Abstract

A nitrocellulose-enzyme immunoassay for the detection of hepatitis A virus (HAV) was developed and shown capable of detecting as little as 1 ng of HAV protein or the equivalent of 1.8 × 10 4 virus particles.

Published
1990

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