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Background Obesity is a cause of FGF21 resistance, which affects the browning and thermogenesis process of the adipose tissue. Decreased receptor expression is influenced by miR‐34a, whose expression is increased in obesity. While FGF21‐based therapies have been widely investigated, the potential activity of Hibiscus sabdariffa Linn. extract (HSE) against FGF21 resistance is unknown. Objective This study aims to determine the effects of HSE on the expression of miR‐34a and FGF21 receptors in white adipose tissue. Methods This experimental study used 24 male Sprague‐Dawley rats and divided into four groups: Control (N); diet‐induced‐obesity rats (DIO); DIO rats with HSE 200 mg/kgBW/day and DIO rats with HSE 400 mg/kgBW/day. Rats were fed a high‐fat diet for 17 weeks. HSE was administered daily for 5 weeks. The administration of HSE 400 mg/kgBW/day resulted in the equivalent expression of miR‐34a to that of the control (p > 0.05). Results FGFR1 receptor expression was also similar to controls (p > 0.05). Beta‐klotho expression was significantly lower than that of control (p, We reported a significant contribution to the literature because we found that H. sabdariffa has the potential to manage FGF21 resistance in DIO rats via the suppression of miR‐34a expression, increasing the number of FGFR1 and beta‐klotho co‐receptors in the adipose tissue. This condition affects the active FGF21 signalling in the process of browning and thermogenesis.

IntroductionPolyphenols have shown capacity to improve appetite sensation, leading to enhanced control of body weight. However, despite being related with hunger-related hormones, metabolic and mechanism are not well known.MethodsThe effectiveness of a nutraceutical composed of extract toLippia citriodoraandHibiscus sabdarrifa(Lc-Hs) for controlling satiety and hunger was analyzed in a cross-over, placebo-controlled (Pla) clinical intervention. The study was divided in two 60-day periods separated by 30-day length wash-out period. At the end of each period, overweight and obese subjects (n = 33; age = 33.76 ± 12.23; BMI = 28.20 kg/m2 ± 2.47; fat mass 30.65 ± 8.39%; both sexes were proposed to eat an ad-libitum meal. Meanwhile, appetite sensation was determined by visual analog scales at different times. Moreover, blood extraction was performed to determine biochemical parameters (lipid and glucidic profile and safety parameters) and to evaluate hunger-related hormones (insulin, leptin, ghrelin, adiponectin, GLP-1 and peptide YY).ResultsA decrease in appetite sensation was observed in Lc-Hs treatment, showing higher satiety quotient (Pla = 3.36 ± 2.33%mm/kcal; Lc-Hs = 5.53 ± 2.91%mm/kcal;p xmin−1; Lc-Hs 8279.73 ± 2745.71%xmin−1;p p p p ConclusionThe supplementation with the Lc-Hs extract decreases appetite sensation in overweight and obese population, reducing calorie intake after an ad-libitum meal. Due to variation on hunger-related hormones and the relationship between satiety feeling, it would be interesting to develop future research focused on the variation of the hormones themselves.

According to traditional medicine, Hibiscus is used to treat hypertension, heart diseases and many other ailments. Commercially available Hibiscus tea is prepared with H. sabdariffa and is expensive. H. rosasinensis is the common variety which is abundant in tropical home-gardens, however the scientific data are lacking on the effects. The present review focuses on the available scientific data on the effects of H. rosasinensis on controlling diabetes and hypercholesterolemia. The endeavour was to identify whether H. rosasinensis flower petals as a ‘tea’ is effective for diabetic and hypercholesterolemic patients. Medical databases such as MEDLINE, BMC, BMJ, research gate, Mendelay literature search database, Google scholar and the general engine Google were searched from November 2018 to April 2019. Search was carried out using keywords such as “Hibiscus rosasinensis”, “antidiabetic effects”, “antihyperlipidemic effects”, “toxic effects”, “anti-inflammatory effects”, “phytochemicals in Hibiscus” etc. Data were critically analyzed to assess whether the effective doses of the research studies on a par with the doses present in H. rosasinensis teas. We found thirty-nine journal articles fulfilled the criteria. The data were categorized and extracted on uses of H. rosasinensis, anti-diabetic effects, anti-inflammatory effects, anti-hypercholesterolemic effects and its phytochemicals. The review revealed that the dose of H. rosasinensis petals present in a home-made Hibiscus tea is theoretically sufficient to elicit anti-hyperglycemic and anti-hyperlipidemic effects. Home-made Hibiscus tea is effective in controlling diabetes and hypercholesterolemia without causing acute toxicity.

This study aimed at reviewing the importance of medicinal plants for the treatment of common hair problems such as baldness, alopecis hair fall, gray hair, dryness and most comman drandruff. The various herbal ingredient are used in the formation are Aloe, Amla, Curry leaves, Camphore, Coconut oil, Eclipta alba, Hibiscus, Heena, Neem, Fenugreek, Sage, Apamarg, Onion, grape seeds, Jatamanshi, Rosamary, Thyme, Tulsi, Garlic and Neem. All ingredients provide essential nutrients such as Vitamin, terpenoids and many essential oils to maintain normal function of sebaceous gland and overall care of skin and hair. Keywords: Hair disease, Medicinal plants, Hair oil

The current study was aimed to investigate the antibacterial and antibiofilm activity of ethanolic extract of Hibiscus sabdariffa L. calyx against locally isolated methicillin-resistant Staphylococcus aureus (MRSA) at different concentrations were started from 0.078 to 40 mg/ml. The extract was prepared by soaked calyces powder of Hibiscus sabdariffa L. with 80% ethanol in the Soxhlet extraction unit, and then it was aseptically filtered. The antibacterial activity was tested by agar diffusion method and broth microdilution method, this method was used to determine the minimum inhibitory concentration of extract, while the antibiofilm activity was determined by using 96-well polystyrene microtiter plates. The results revealed that the ethanolic extract has antibacterial activity in a concentration-dependent manner, the average diameter zone of inhibition observed against MRSA isolates ranged from 14±0.5 mm to 20±0.5 mm Moreover, at sub-inhibitory concentration, this extract developed an isolate-specific antibiofilm effect and presented highly significant (P< 0.05) variability in biofilm formation before and after addition of ethanolic extract, Mr1, and Mr7 isolates were gave the lowest and highest antibiofilm activity, respectively. In conclusion, the ethanolic extract of H. sabdariffa L. calyx is a promising alternative medication that can be used to treat the infection caused by MRS.

Hibiscus exhibits high variation in chromosome number both within and among species. The Hibiscus mutabilis L. karyotype was analyzed in detail using fluorescence in situ hybridization (FISH) with oligonucleotide probes for (AG3T3)3 and 5S rDNA, which were tested here for the first time. In total, 90 chromosomes were counted in prometaphase and metaphase, and all exhibited similarly intense (AG3T3)3 signals at both ends. (AG3T3)3 showed little variation and thus did not allow discrimination among H. mutabilis chromosomes, but its location at both ends confirmed the integrity of each chromosome, thus contributing to accurate counting of the numerous, small chromosomes. Oligo-5S rDNA marked the proximal/distal regions of six chromosomes: weak signals on chromosomes 7 and 8, slightly stronger signals on chromosomes 15 and 16, and very strong signals on chromosomes 17 and 18. Therefore, 5S rDNA could assist in chromosome identification in H. mutabilis. Metaphase chromosome lengths ranged from 3.00 to 1.18 μm, indicating small chromosomes. The ratios of longest to shortest chromosome length in prometaphase and metaphase were 2.58 and 2.54, respectively, indicating karyotype asymmetry in H. mutabilis. These results provide an exact chromosome number and a physical map, which will be useful for genome assembly and contribute to molecular cytogenetics in the genus Hibiscus.

Aim: To evaluate the ability of red and white hibiscus (H) in improving the epithelial barrier in indomethacin-induced duodenal and colonic inflammation, compared with prebiotic and to investigate whether the red and/or white hibiscus can be used as natural prebiotic like agents. Methods: Histological, histomorphometric, histochemical demonstration for goblets cells and mucin intensity. Immunohistochemical demonstration of COX-2, and tight junctions (Claudin-1 and E-cadherin) were examined. Duodenal and colonic TNF-α, IL-6, IL-10, PGE2, MPO, MDA, TAC, besides, serum CRP were evaluated. Results: Indomethacin induced inflammation and ulceration in the duodenum and colon, with significant depletion in goblet cell count and mucin intensity. COX-2 was increased while claudin-1 and E-cadherin were significantly diminished. Tissue TNF-α, IL-6, MPO, MDA, and serum CRP were elevated significantly by indomethacin, while IL-10, PGE2 and TAC were reduced. Co-treatment with red H, white H, or prebiotics plus indomethacin improved significantly duodenal and colonic histoarchitecture, tissue contents of PAS cells, mucin, claudin-1 and E-cadherin, while COX-2 reduced. TNF-α, IL-6, MPO, MDA, and serum CRP were decreased significantly, while IL-10 , PGE2 and TAC were restored. Conclusion: The current research introduces red and white hibiscus as prebiotics-like agents because of their anti-inflammatory, antioxidant, or tight junctions modulating activities.

The conviction that herbal drugs have enormous health benefits has led to increase the rate of their consumption by Nigerians. The aim of this study was to assess the carcinogenic property of some popularly consumed anti-diarrheal herbal drugs via polycyclic aromatic hydrocarbons (PAHs) quantification. Three prevalent anti-diarrhea herbal drugs , Odunmo herbal drug (Hibiscus rosa-sinensis and Bacopamonnieri), Orogun herbal mixture (Hibiscus sabdariffaI and Hedera helix), and Alora herbal syrup (Aloe vera and Hibiscus sabdariffaI) were bought for the purpose of this study and they were coded as samples A, B, and C, respectively. The ultrasonic extraction of the herbal drugs was carried out using standard procedures. The crude extracts obtained were purified using a chromatographic method. The concentrations of PAHs were quantified using gas chromatography flame ionization detector (GCFID). The diagnostic indices, group distribution, toxicity equivalence and exposure dosage were estimated. The cancer risk values were theoretically speculated based on concentrations of PAHs in the tested herbal drugs, associated with the published estimates of each concentration to cause cancer and the calculated exposure doses of the anti-diarrhea herbal drug samples were within three age groups (children, Preteen and adult). The highest concentration of total PAHs was observed in sample A (58.2815mg/kg) and the lowest concentration in sample B (44.1898mg/kg), but the concentration of total PAHs in sample C was 47.4169 mg/kg. The highest percentage of carcinogenic PAHs in the anti-diarrheal herbal drugs was found in sample C (48.66%) and the lowest in sample B (38.17%). The diagnostic indices confirmed a pyrogenic source of PAHs. Group distribution of PAHs showed that the herbal drugs are weakly carcinogenic due to high concentrations of low and moderate molecular weight PAHs. The cancer risk estimated for all the age groups where below the limit established by the United State Environmental Protection Agency (USEPA) for cancer (1 x 10-6). This ascertained that the use of these herbal drugs cannot cause cancer. However, consumers of these herbal drugs should take necessary precautions as excessive intake can lead to dangerous health implications.

Hibiscus calyx presents high content of bioactive compounds, such as anthocyanins and other phenolic compounds responsible for antioxidant activity. The present study investigated how the tea infusion temperature (hot and cold) can change the phytochemical profile, antimicrobial activity, and stability of hibiscus tea. The results indicated the same phytochemical profile for cold (infusion during 2 h/25 °C) and hot (7 min/75 °C) tea by using FTIR and UPLC-MS/MS analyses. Hibiscus tea, hot or cold, showed antimicrobial activity against strains of Salmonella Typhimurium, Pseudomonas aeruginosa, Escherichia coli and Staphylococcus, with minimum inhibitory concentration (MIC), obtaining values between 62.5 and 125.0 mg/ml. The hot infusion was more efficient for the bioactive compounds extraction, being 47% higher for total anthocyanins. After 48 h at 5 °C, the anthocyanin content had an average decrease of 30% (cold tea) and 48% (hot tea). Tea cream content increased to 15% (cold tea) and 37% (hot tea) after 72 h storage. Regarding the color, it was observed that after 48 h storage there was a clearing of the teas, and the L* coordinate increased from 21.03 to 24.93, and 20.72 to 24.90 for cold and hot tea, respectively. Our study indicates that hibiscus tea provides more antioxidants when prepared in the hot condition (7 min at 75 °C), and soon after cooled to 5 °C. Finally, it is recommended that hibiscus tea be consumed up to 24 h from preparation even when kept refrigerated.

Kenaf (Hibiscus cannabinus L.), an annual herbaceous plant in the Malvaceae family, has become a multifunctional crop in Malaysia due to its large number of industrial applications for its fibrous stem. Recently, its kenaf leaves that have high antioxidant properties are getting more attention to be developed into tea. Therefore, this research aims to determine the best brewing time and temperature based on the physical properties, antioxidant activities and sensory of kenaf leaves tea (KLT). The kenaf leaves powder which was infused in hot (80 °C or 100 °C; 5 min or 10 min) or cold water (room temperature; 60 min or 120 min) were analysed. Results demonstrated that the KLT brewed at 80 °C for 10 min and 100 °C for 10 min showed the highest antioxidant activities in most of the antioxidant analysis conducted. Moreover, the colour of cold-brewed KLT was much lighter than the hot-brewed KLT and the cold-brewed KLT (room temperature; 120 min) can likely be a new trend for the consumer since it contained high antioxidising capabilities. However, the pH, greenness, yellowness, sensory results in KLT were not affected significantly by both brewing time and temperature (p > 0.05). The antioxidant test was correlated positively with the phytochemical contents but insignificant relationship with most of the colour parameters. Overall, the optimum temperature and time for brewing KLT was 80 °C at 10 min because it saved energy and extracted the highest amount of antioxidants while retaining similar sensory taste with other brewing conditions.

This study is on the nutritional efficiency of the host plants viz., Corchorus olitorius, C. capsularis, Hibiscus cannabinus, H. sabdariffa, Crotoloria juncea and Boemheria nivea and their biochemical influences on Bihar hairy caterpillar Spilosoma obliqua (Walker) (Lepidoptera: Arctiidae). The results revealed that the nutritional and biochemical parameters varied significantly with the infested and uninfested host plants. The growth rate of 5th instar larvae varied from 13.06 mg/ day in B. nivea to 17.90 mg/ day in C. olitorius; it was 14.80 ± 0.69, 16.74 ± 0.74, 14.94 ± 0.29 and 15.06 ± 0.26 mg/ day on C. capsularis, H. cannabinus, H. sabdariffa, C. juncea and B. nivea, respectively. Thus, C. olitorius appeared to be the most suitable food source for growth and development of S. obliqua. Peroxidase and polyphenol oxidase were elicited by the infestation of S. obliqua indicating their role in insect defence. Higher phenol and peroxidase content in the host plants adversely affected the food utilization indices. This is the possible explanation for variation in food consumption and development of S. obliqua; and it might be due to significant differences in the biochemical contents of the evaluated bast fibre crops.

Roselle—Hibiscus sabdariffa L. leaves at different stages of growth, calyces and seeds were analyzed for nutritional and anti-nutritional factors. All the treatments contained a good but varied proportion of carbohydrate in a range of 26.93–54.13%, crude protein from 5.7 to 27.06%, crude fat 1.16–13.09%, crude fibre 15.75–36.10%, energy 631.36–1065 kJ, ash 6.08–13.74% and moisture content 6.00–9.7%. The vitamins A, C and E were also found to be present in all the treatments in a different but substantial amount. The calcium, magnesium and iron contents in all the treatments were higher than the recommended daily allowance of 1250, 350, and 15 mg for adults, while the phosphorus and sodium values in all the treatments were below 1000 and 1500 mg RDA for adults. However, the Na + /K + for all the treatments were lower than 1. The values of anti-nutrients in the samples were small except post-flowering red which had high phytate content of 21.02%, although this can be easily reduced during processes like boiling and cooking. Thus, both cultivars of roselle contain high nutritional, elemental, and vitamins compositions and small content of anti-nutrients.

The calyces of roselle ( Hibiscus sabdariffa L.) are used to make a refreshing drink with high content of anthocyanins and other phenolic compounds, although the process for obtaining the beverage is not standardized. In this research it was determined physicochemical characteristics, total soluble polyphenols content, antioxidant activity and acceptance for beverages prepared by decoction at four concentrations of calyces in water (1.0, 2.5, 5.0 and 10.0%) and two concentrations of sucrose (11 and 16%). Color parameters in beverages permitted to describe them as red, which turned darker as the content of hibiscus increased. Total soluble polyphenols content and antioxidant activity were directly correlated with content of calyces in beverages and inversely correlated with pH, which means that beverages with higher content of calyces could be perceived as more acidic and more intense in characteristics associated with hibiscus presence, such as acid, astringent and the presence of intense dark red color, although the higher concentration of sucrose might have contributed to mask slightly the sourness and astringency. Acceptance for hibiscus beverages allowed to observe two segments of consumers, high-intensity and low-intensity likers but both confluence in overall liking values for beverages prepared with 2.5% calyces and 16% sucrose.

Background Inadequate breastmilk production is one of the key factors associated with suboptimal breastfeeding. In most local African homes, special herbs and some food items are commonly used to promote breastmilk production (known as lactogogue/galactogogue). We describe the use and characterize the herbs and food items used to promote breastmilk production in two regions of Ghana. Methods We conducted a cross-sectional study in 2018 involving 402 lactating mothers. The range of foods used as lactogogues was obtained from 20 participants through focus group discussions. Quantitative data on demographics, lactogogue use and feeding practices were obtained through questionnaire administration. Results The mean age of women was 29.2 years and children were 10 months. Breastmilk production problems were low (22.4%) and the majority of lactating mothers felt they had adequate breastmilk (70.4%) but awareness about lactogogues was widespread in both regions (88.8%) and highest in the Brong-Ahafo region (90.0%). Information about lactogogues was mainly from grandparents (24.6%), parents (31.6), health facilities (16.5%) and friends (12.8%), while the media had little influence (Bra leaves (Hibiscus sabdariffa), hot black tea, Werewere/Agushi (Citrulus colocynthis) prepared with Bra leaves, and Abemudro (a polyherbal formulation). Only 13.2% of lactating mothers also used lactogogues during pregnancy. Conclusions Special foods and selected herbs are widely used to enhance breastmilk production in Ghana and constitute an important part of the diet of lactating mothers. These results could contribute to understanding breastfeeding behaviours and stimulate further research into evaluating the safety and scientific efficacy of these products in Ghana.

Biodegradable additives are required to minimize the environmental hazards from drilling fluid wastes. This study explores the feasibility of the applications of henna leaf extracts (HLE) and hibiscus leaf extracts (HBLE) as ecological benign products in water-based drilling fluids (WBDFs). Rheological and filtration characterizations were carried out on the WBDFs to detect the effects of different concentrations (1, 2, 10, 20, 30, and 40 g) of these plant extracts at 78 and 300 °F. The results of 1 and 2 g of the plant extracts were compared with those of low-viscosity polyanionic cellulose (PAC LV). Compatibility test was carried out using 25 g/L of the green additives on base fluid (A-0), and the swelling rate of sodium bentonite in distilled water was also considered using 1, 10, and 20 g of the green additives. The findings showed that HLE and HBLE significantly reduced the filtrate loss between 62% and 67% and between 64% and 76%, respectively, and improved the rheological characteristics of the WBDF system between 10 and 40 g. PAC LV showed a greater effect on the rheological properties than the green additives in equal amounts (1 and 2 g), but it exhibited flat high and progressive gels which can lead to mechanical pipe sticking. The test data also showed that the inclusion of HLE and HBLE in the WBDF demonstrated larger impact on the mud cake than PAC LV. The cake thickness of the WBDF was reduced in the following order: 30–32% (by HLE), 32–33% (by HBLE), and 24–27% (by PAC LV). This interprets the outstanding filtration characteristics of green additives. Further, compatibility test data confirmed that the green additives are compatible with the other base fluid additives and the swelling behavior of sodium bentonite verified that the green plants are effective in inhibiting bentonite swelling. Here, the Henna extracts displayed higher inhibition property than the Hibiscus product. Notwithstanding, both products showed excellent inhibition property and a strong viscosity enhancing effect on the WBDF system.

Kenaf (Hibiscus cannabinus L.) seed is an underutilized protein-rich resource considered as a by-product of the kenaf fiber processing industry. Its high protein content (34%) makes it a promising candidate as a source of bioactive protein hydrolysates. In this study, the potential of enzymatically hydrolyzed kenaf seed protein to generate multifunctional bioactive peptides was evaluated. Kenaf seed protein concentrate was hydrolyzed using four different proteolytic enzymes (papain, alcalase, bromelain, and flavourzyme) at their respective optimum pH and temperature. The choice of enzyme affected the bioactivities to a certain degree as KSPH were shown to possess high ACE inhibitory activity and low-to-moderate DPP-IV and antioxidant activity. Papain KSPH showed the highest ACE inhibitory activity with 95% inhibition compared to other enzymatic hydrolysates, and therefore was chosen for further investigation of its antihypertensive activity. Papain KSPH was profiled for its hydrophobicity by RP-HPLC and revealed that the majority of late-eluting fractions exerted the highest ACE inhibitory activity. Spontaneously hypertensive rats showed a decrease of approximately 18–46 mmHg in their systolic blood pressure (BP) from 0 to 24 h after oral administration of papain KSPH at dosages of 100 mg/kg, 300 mg/kg, and 500 mg/kg. However, the effect was not dose-dependent. As a novel protein source, future research should aim to demonstrate the safety of kenaf seed protein and its hydrolysates, and validate its bioactivity through human intervention trials. Overall, kenaf seed protein has the potential to generate antihypertensive hydrolysates with multifunctional bioactivities as part of a functional food ingredient.

Introduction: Tuberculosis is an infectious disease caused by Mycobacterium tuberculosis. The leading cause of death worldwide is responsible for 1.5 million deaths each year. The cause of tuberculosis infection is the tubercle bacillus discovered by Robert Koch in 1882. The organism causing it is Mycobacterium tuberculosis.The spread of new cases is divided in several regions including Southeast Asia and Africa, which are the regions that have the biggest contribution to the prevalence of tuberculosis. So it takes pillars and components of TB control that cover all lines. Method: This study aims to discuss the potential of herbal plants spread in Indonesia as herbal medicines that have the potential to treat tuberculosis infection. This study uses a literature study method which prepares the same as other research, but the sources and methods of data collection are by taking data from the library, reading, taking notes, and processing research materials. Result: One of the components that are currently being developed in TB infection intervention methods is the development of herbal medicines for tuberculosis control. The herbal medicines used come from garlic (Allium sativum), Centellaasiatica, beluntas leaves, Hibiscus rosasinensis L., Boechmeria virgate (Forst) Guill, tobacco leaves, Morindacitrifolia L., Javanese wood bark, Spondiaspinnata (Lf)Kurz. Conclusion: The herbal medicinal ingredients contain major chemicals such as alicin, ajoene, asiaticoside, asiatic acid, madecassic acid, and madecassoside, alkaloids, flavonoids, tannins, saponins, phenolic, and triterpenoids which have the ability to have a bactericidal effect to kill the Mycobacterium tuberculosis bacteria.

Hibiscus cannabinus L. or commonly known as kenaf is a multipurpose crop with various medicinal and nutritional properties. The aim of this study was to determine the antioxidant, nutritional, and functional properties of kenaf leaves powder. In addition, the effect of kenaf leaves powder fortification in bread properties and consumer acceptability were evaluated by production of three formulations of bread with 0% (control), 4% and 8% substitution of kenaf leaves powder per weight of flour used. From the sensory evaluation, 4% leaves powder bread was the most preferred by the panelists followed by 0% leaves powder bread then 8% leaves powder bread. Therefore, 4% leaves powder bread was chosen as the best formulation for this study and it showed significantly higher value of DPPH free radical scavenging capacity (8.05 mg TE/100 g), total phenolic content (12.9 mg GAE/100 g) and total flavonoid content (13.3 mg QE/100 g) compared to control bread (1.38 mg TE/100 g, 8.17 mg GAE/100 g and 8.77 mg QE/100 g, respectively). Besides, 4% leaves powder bread also showed improvement in calcium and total dietary fibre compared to control bread. Given these results, kenaf leaves powder can be used in baking formulation up to 4% with flour to enhance the functional properties of baking formulation without compromising eating quality.

Aim: This study aimed to analyze the physicochemical parameters variation of the homemade hibiscus drink (Hibiscus sabdariffa) after contact with the oral cavity. Methods and materials : The study took place from October 2010 to March 2012 at the F´elix Houphou¨et Boigny University in Cocody, Cˆote d’Ivoire. The procedures were carried out at the Laboratory of Analytical Chemistry and Bromatology of the Training and Research Unit in Pharmaceutical and Biological Sciences, the Biochemistry Laboratory of the Emergency Medical Aid Service and the National Laboratory of Public Health (LNSP). Potential hydrogen (pH), acidity, calcium, phosphorus and sugar were the analyzed variables. Results: The pH in drink discharges does not vary over time. Drink discharges have, over time, lower calcium and higher phosphorus content than the starting drink. Conclusion: Hibiscus drink would have an acid generating effect on the tooth, but its high concentration of calcium and phosphorus would counterbalance this effect and could protect teeth against demineralization. Keywords: Acidity, Dental contact, Calcium, Hibiscus drink, Phosphorus, Potential hydrogen, Sugars.

Roselle seeds, a waste biomass of the roselle calyx processing industry, were utilized to recover valuable compounds of oil, vitamin E, and water-soluble saccharides. Firstly, ultrasound-assisted extraction (UAE) and conventional stirring extraction were conducted for saccharide extraction, and the advantage of UAE was confirmed. Secondly, oil, vitamin E, and saccharides extracted from Vietnamese roselle seeds by UAE were analyzed for the first time. Oil of tri-, di-, and mono-glycerides, fatty acids of linoleic-, oleic-, palmitic-, and stearic-acids, vitamin E of γ- and α-tocopherol, and saccharides of sucrose, raffinose, stachyose, etc. were identified, and the amounts of these components were compared with those in other country's roselle seeds. Thirdly, cascade extraction of oil, vitamin E, and saccharides by UAE was investigated with solvents of hexane, hexane:ethyl acetate binary solvent, and water. The results indicated that the order of using solvents was very important for high and selective extraction: the best order to recover oil (almost 100%), vitamin E (95.7%), and saccharides (86.2%) was hexane, and then water.

Titanbicus (TB), a hybrid of Hibiscus moscheutos × H. coccineus (Medic.) Walt., has potential to be used as an edible flower. In this study, proximate nutritional content, anthocyanin content, total polyphenol content (TPC), and antioxidant activities in vitro and in vivo were investigated. Three cultivars of TB, namely Artemis (AR), Rhea (R), and Adonis (AD), were used as materials. Protein and carbohydrates were the primary macronutrients, while crude fat and ash were detected in trace amounts. Cyanidin 3-glucoside (Cy3-G) and cyanidin 3-sambubioside (Cy3-Sam), were identified in all TBs. The highest anthocyanin content was observed in AD (47.09 ± 1.45 mg/g extract), followed by R and AR (6.04 ± 0.20 and 2.72 ± 0.11 mg/g extract, respectively). The TPC of AD (225.01 ± 1.97 mg/g extract) was greater than that of AR and R (185.41 ± 3.24 and 144.10 ± 1.71 mg/g extract, respectively). AD exhibited the strongest in vitro antioxidant activity in hydrophilic oxygen radical absorbance capacity, compared to the other two TBs. In addition, AD extract suppressed the generation of reactive oxygen species in caudal fin of wounded zebrafish. Antioxidant activities of AD appeared to be related to its total anthocyanin content, Cy3-G, Cy3-Sam, and TPC. Our findings indicate that TB, particularly the AD cultivar, would be an attractive source of bioactive compounds with antioxidant activities, and can improve both nutritional value and appearance of food.

Hibiscus rosasinensis, medicinal plant known for its various medicinal properties. The present study was to identify and characterize the flavonoid compound from the petals of Hibiscus rosa sinensis. The antioxidant compounds were isolated by Silica Gel G Column chromatography. From the fractions, C5 shows the highest antioxidant activity compared to C3 & C4 compounds. DNA damage protection activity results indicated that two major bands super coiled DNA and open circular DNA was protected by the presence of different concentration (5 µg, 10 µg, 15 µg & 20 µg) of C5 compound isolated from the petals of Hibiscus rosa sinensis. IR absorptions peaks reveals the presence of functional groups alcohols, phenols, α,β unsaturated aldehyes, ketones and alkanes. The structure of compound was characterized as Hibiscetin-3-glucoside (C21H20O14) by Mass spectroscopy and NMR. The study emphasized flavanoid compounds have effective scavenging activities which may be due to its phenolics and flavonoid contents and can be used as anticancer agents. Keywords: Flavanoid, Antioxidant, DNA protector activity, IR, NMR, Hibiscetin-3-glucoside

Background: The condition of hyperglycemia in Diabetes mellitus (DM) causes a disruption of the function of phagocytes that can facilitate M. tuberculosis infection. Immunostimulant from natural ingredients can help improve the condition of hyperglycemia an immune function. Objective: To analyze the effect of Hibiscus rosa-sinensis L. extract on fasting blood glucose level and the number of mast cell in TB-DM mouse model. Methods: This experimental study used 27 male Wistar rats, 8 weeks of age, 200-230 g weight, divided into 3 groups, K: Normal control group, P1: TB-positive mouse group P2: Group rat TB-DM + Hibiscus rosa-sinensis L extract dose 250 mg/kgBW and 3 time measurement of H + 7 DM, H + 14 DM and H + 21 DM. TB-DM model mice were prepared with the administration of nicotinamide (NA) 100 mg/kgBW and streptozotocin (STZ) 65 mg/Kg BW, TB agent 1.5 x 105 CFU. Fasting blood glucose levels were measured by GOD-PAP method and Pulmonary tissue is excised, made in paraffin blocks and stained with blue Toluidine to count the mast cells. Results: There was an improvement in the mean fasting blood glucose levels in the group treated with the Hibiscus rosa-sinensis L. extract of 250 mg/kgBW compared to the TB-DM group without extract therapy followed by decreased the number of mast cell. Conclusion: Hibiscus rosa-sinensis L. extract. improves fasting blood glucose levels and decreases the number of mast cell of TB-DM rat model.

A real-time impedance-based electrochemistry assay was used for assessing the cytotoxicity effect of water extract Hibiscus Rosa-Sinensis on Vero cell line. In this study, H. rosa-sinensis was extracted by using the water extraction with three different concentration which were 200, 100 and 50 µg/mL. The phytochemical compounds in the extract were analyzed by using the Gas Chromatography-Mass Spectrometry (GC-MS). The GC-MS result showed that the extract contains bioactive compounds such as n-hexadecanoic acid, pentadecanoic acid, phenol, 2,4-bis(1,1-dimethylethyl) and octadecanoic acid that have bioactive properties such as cytotoxic and antioxidant. The cytotoxic effect of the extract on normal cell line was assessed by using Real-Time Cell Analysis (RTCA) instrument. The result showed that there was no significant cytotoxicity effect against Vero cell lines. This result showed that the real-time impedance-based assay can be utilized to monitor changes of the cells and to determine the inhibitory concentration of the extract.

This study sought to summarize clinical evidence of sour tea (Hibiscus sabdariffa L.) administration on cardiovascular disease risk factors. PubMed, Scopus, Institute for Scientific Information Web of Science, and Google Scholar were systematically searched from inception to June 2019 to identify randomized clinical trials, which assessed the effect of sour tea consumption on lipid profiles, fasting plasma glucose, and blood pressure in adult populations. Mean and standard deviation for each parameter were extracted to calculate effect size. Cochrane Collaboration tools were used to evaluate risk of bias assessment. A total of seven randomized clinical trials consisting 362 participants were included in the meta-analysis. Pooled effect size demonstrated that sour tea consumption significantly reduces fasting plasma glucose (-3.67 mg/dl, 95% confidence interval, CI [-7.07, -0.27]; I2 = 37%), systolic blood pressure (-4.71 mmHg, 95% CI [-7.87, -1.55]; I2 = 53%), and diastolic blood pressure (-4.08 mmHg, 95% CI [-6.48, -1.67]; I2 = 14%). Although no significant effect was observed on triacylglycerol, total cholesterol, and high-density lipoprotein cholesterol following sour tea consumption, a trend toward a significant reduction was found in low-density lipoprotein cholesterol serum concentrations (p = 0.08). This systematic review and meta-analysis suggests that sour tea consumption could have beneficial effect in controlling glycemic status and blood pressure among adult population.

Objectives: To evaluate wound healing activity of herbal ointment containing the extracts of Hibiscus rosa-sinensis flowers and Curcuma longa rhizomes. Methodology: H. rosa-sinensis flowers and C. longa rhizomes were extracted using 95% ethanol. H. rosa-sinensis and Hibiscus rosa-sinensis ointment were prepared using paraffin wax, lanolin and petroleum jelly. Ointment formulation was applied once daily on the excision wound made on Sprague Dawley rats for 20 days. Results: On day 20, the treatment group showed a significant increase in wound contraction which was 93.52 ± 1.38% when compared to control (87.62 ± 1.48%). Conclusion: Herbal ointment exhibited significant wound healing activity in rat excision wound model.

Phytochemicals are essential raw materials for the production of formulations that can be helpful in crop protection. In particular, Hibiscus spp., which are often used in traditional medicine, are rich in potential bioactive molecules. This study presents an analysis of the thermal, vibrational, and phytochemical characteristics of a light purple variety of Hibiscus syriacus, using thermal gravimetric and differential scanning calorimetry, Fourier-transform infrared spectroscopy, and gas chromatography-mass spectroscopy techniques. Further, with a view to its valorization, the antimicrobial activity of its extracts has been investigated in vitro against Erwinia&nbsp, amylovora (the phytopathogen responsible for fire blight in apples, pears, and some other members of the family Rosaceae), Erwinia vitivora (the causal agent of the “maladie d’Oléron” in grapevines), and Diplodia seriata (responsible for “Bot canker”). Higher heating values and thermal features showed similarities with kenaf biomass. The main compounds identified in the hydro-methanolic extracts were: in flowers, 1-heptacosanol, heptacosane, 1-tetracosanol, hexadecenoic acid, 9,12,15-octadecatrienoic acid, and 9,12-octadecadienoic acid, and in leaves, the coumarin derivative 4,4,6,8-tetramethyl-2-chromanone, vitamin E, phytol, and sitosterol. MIC values of 500 and 375 μg·mL−1 were obtained against E. amylovora for flower and leaf extracts, respectively, upon conjugation with chitosan oligomers (to improve solubility and bioavailability). In the case of E. vitivora, MIC values of 250 and 500 μg·mL−1, respectively, were registered. Regarding the antifungal activity, EC90 values of 975.8 and 603.5 μg·mL−1, respectively, were found. These findings suggest that H. syriacus (cv. ‘Mathilde’) may be a promising source of antimicrobials for agriculture.

Background: In our previous study, Hibiscus syriacus leaf tissue was successfully cultivated in an optimized callus culture system, and subsequently extracted with 70% ethanol to prepare H. syriacus callus extract (HCE). The previous study suggested that the callus culture is useful method for obtaining the anti-inflammatory ingredients from H. syriacus. Purpose: In the present study, we aimed to investigate the effect of HCE on the colorectal cancer (CRC) and its underlying mechanism of action using HT-29 cells and thymus-deficient mice bearing HT-29 xenografts. Methods: The cytotoxicity of HCE was investigated by MTT and colonies formation. The underling mechanism by which HCE regulates specific proteins in HT-29 cells was evaluated by the proteomic analysis. These putative proteins were validated using qRT-PCR and immunoblotting analyses. Subsequently, oral administration of HCE for 15 days further evaluating the anti-tumor activity by mRNA and protein expressions levels and tumor histopathology. Results: Results of cell viability and colony formation assays revealed a significant cytotoxic effect of HCE at doses below 100 μg/mL against HT-29 cells, but not against normal cells. Through differential protein expression analysis, signaling pathways underlying anti-CRC activity were predicted in HCE-treated HT-29 cells: Notch signaling, cholesterol biosynthesis, and AMPK signaling pathways. qRT-PCR and immunoblotting analyses indicated that the cytotoxic effect of HCE against HT-29 cells might be associated with the suppression of Notch signaling, which positively contributes to cholesterol biosynthesis. To our knowledge, this can be presented as the first study to demonstrate the detailed relationship between Notch signaling and cholesterol-AMPK signaling. Our in vivo result further corroborated the in vitro finding that 100 and 200 mg/kg HCE for 15 days exerts its anti-cancer effect via Notch signaling-mediated suppression of cholesterol synthesis without systemic toxicity. Conclusion: Our findings can serve as a starting point for developing the novel anti-CRC agent using HCE, as a targeted medicine acting on regulating Notch signaling and cholesterol synthesis.

Development of strategies for removing heavy metals from aquatic environments is in high demand. Cadmium is one of the most dangerous metals in the environment, even under extremely low quantities. In this study, kenaf and magnetic biochar composite were prepared for the adsorption of Cd2+. The synthesized biochar was characterized using (a vibrating-sample magnetometer VSM), Scanning electron microscopy (SEM), X-ray powder diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), and X-ray photoelectron spectroscopy (XPS). The adsorption batch study was carried out to investigate the influence of pH, kinetics, isotherm, and thermodynamics on Cd2+ adsorption. The characterization results demonstrated that the biochar contained iron particles that help in improving the textural properties (i.e., surface area and pore volume), increasing the number of oxygen-containing groups, and forming inner-sphere complexes with oxygen-containing groups. The adsorption study results show that optimum adsorption was achieved under pH 5–6. An increase in initial ion concentration and solution temperature resulted in increased adsorption capacity. Surface modification of biochar using iron oxide for imposing magnetic property allowed for easy separation by external magnet and regeneration. The magnetic biochar composite also showed a higher affinity to Cd2+ than the pristine biochar. The adsorption data fit well with the pseudo-second-order and the Langmuir isotherm, with the maximum adsorption capacity of 47.90 mg/g.

This study aimed to assess the efficacy of Hibiscus sabdariffa (Roselle) in regulating blood lipids among patients with metabolic syndrome and related disorders. PubMed, the Cochrane Library, Embase, Web of Science, and Clinical Trials were searched to identify the randomised controlled trials meeting the inclusion criteria. Study selection, data extraction, and risk assessment were performed according to Cochrane handbook; available data were analysed using STATA 15.0 software. Eventually, nine trials involving 503 participants were included in this meta-analysis. The results showed that compared with the control group, H. sabdariffa supplementation could reduce total cholesterol (WMD = -14.66; 95% CI [-18.22, -11.10]; p = .000; I2 = 46.9%) and low-density lipoprotein cholesterol (WMD = -9.46; 95% CI [-14.93, -3.99]; p = .001; I2 = 50.1%) but could not effectively reduce triglyceride (WMD = -0.77; 95% CI [-7.87, 6.33]; p = 0.832; I2 = 0%). Meanwhile, there were no serious adverse reactions reported in the included studies. To summarise, current evidence suggests that the benefits of H. sabdariffa supplementation to patients with metabolic diseases are associated with its cholesterol-lowering effects; however, more high-quality clinical trials are needed to confirm these results.

Beneficial therapeutic effects of phenolic acids have been proven in various research projects including in vivo and in vitro studies. Gentisic acid (GA) is a phenolic acid that has been associated with useful effects on human health, such as antiinflammatory, antigenotoxic, hepatoprotective, neuroprotective, antimicrobial, and especially antioxidant activities. It is an important metabolite of aspirin and also widely distributed in plants as a secondary plant product such as Gentiana spp., Citrus spp., Vitis vinifera, Pterocarpus santalinus, Helianthus tuberosus, Hibiscus rosa-sinensis, Olea europaea, and Sesamum indicum and in fruits such as avocados, batoko plum, kiwi fruits, apple, bitter melon, black berries, pears, and some mushrooms. This study was undertaken to review the pharmacological effects, pharmacokinetic properties as well as toxicity and pharmaceutical applications of GA.

Objectives: To assess the use of Hibiscus sabdariffa (HS), known as karkade in Jordan, in cases of uncontrolled hypertension, either with or without medication, as validated by several clinical tri...

Disulfide-rich plant peptides with molecular masses of 2-6 kDa represent an expanding class of peptidyl-type natural products with diverse functions. They are structurally compact, hyperstable, and underexplored as cell-penetrating agents that inhibit intracellular functions. Here, we report the discovery of an anionic, 34-residue peptide, the disulfide-rich roseltide rT7 from Hibiscus sabdariffa (of the Malvaceae family) that penetrates cells and inhibits their proteasomal activities. Combined proteomics and NMR spectroscopy revealed that roseltide rT7 is a cystine-knotted, six-cysteine hevein-like cysteine-rich peptide. A pair-wise comparison indicated that roseltide rT7 is >100-fold more stable against protease degradation than its S-alkylated analog. Confocal microscopy studies and cell-based assays disclosed that after roseltide rT7 penetrates cells, it causes accumulation of ubiquitinated proteins, inhibits human 20S proteasomes, reduces tumor necrosis factor-induced IκBα degradation, and decreases expression levels of intercellular adhesion molecule-1. Structure-activity studies revealed that roseltide rT7 uses a canonical substrate-binding mechanism for proteasomal inhibition enabled by an IIML motif embedded in its proline-rich and exceptionally long intercysteine loop 4. Taken together, our results provide mechanistic insights into a novel disulfide-rich, anionic, and cell-penetrating peptide, representing a potential lead for further development as a proteasomal inhibitor in anti-cancer or anti-inflammatory therapies. Ministry of Education (MOE) Nanyang Technological University Published version This work was supported in part by Nanyang Technological University Internal Funding–Synzymes and Natural Products (SYNC) and AcRF Tier 3 funding Grant MOE2016-T3-1-003

Since ancient Egypt, orthosis was generally made from wood and then later replaced with metal and leather which are either heavy, bulky, or thick decreasing comfort among the wearers. After the age of revolution, the manufacturing of products using plastics and carbon composites started to spread due to its low cost and form-fitting feature whereas carbon composite were due to its high strength/stiffness to weight ratio. Both plastic and carbon composite has been widely applied into medical devices such as the orthosis and prosthesis. However, carbon composite is also quite expensive, making it the less likely material to be used as an Ankle-Foot Orthosis (AFO) material whereas plastics has low strength. Kenaf composite has a high potential in replacing all the current materials due to its flexibility in controlling the strength to weight ratio properties, cost-effectiveness, abundance of raw materials, and biocompatibility. The aim of this review paper is to discuss on the possibility of using kenaf composite as an alternative material to fabricate orthotics and prosthetics. The discussion will be on the development of orthosis since ancient Egypt until current era, the existing AFO materials, the problems caused by these materials, and the possibility of using a Kenaf fiber composite as a replacement of the current materials. The results show that Kenaf composite has the potential to be used for fabricating an AFO due to its tensile strength which is almost similar to polypropylene's (PP) tensile strength, and the cheap raw material compared to other type of materials.

Hibiscus surratensis L. calyces essential oil can be used as potential natural antibacterial. In this study, composition of essential oil obtained from calyces of Hibiscus surattensis L. and the antibacterial effect of this essential oil on some important food-borne pathogens were examined. Essential oil of hibiscus calyces obtained from hydro-distillation method were analyzed by GC-MC. As a result of the analyses main compounds, β-caryophyllene (11.68%), menthol (9.44%), methyl salicylate (8.32%), camphor (8.47%) and germacrene D (5.13%) were identified in essential oil of calyces of the Hibiscus surattensis L. plant. Antibacterial effects of calyces essential oils were determined by disc diffusion method. Also minimal inhibitory concentration and minimal bactericidal concentration values were determined. The most powerful antibacterial effect was observed on Listeria monocytogenes with a diameter zone of 25.26 mm, followed by a diameter zone of 23.42 mm on Staphylococcus aureus and with a diameter zone of 22.93 mm on Escherichia coli (*P

The ultrasound assisted extraction (UAE) of total anthocyanins (TA) and total phenolic compounds (TP) from hibiscus calyces was evaluated with the intention of using the extract in future food products. The Box–Behnken Design was used for the extraction evaluating sonication time, solvent concentration and temperature. Final results suggest that 45 min, 65 °C and 25% ethanol can be considered the best region for UAE extraction. The relative area of the peaks identified by UPLC-MS/MS in the extract obtained by the UAE are superior in 2 or even 4 times those obtained by conventional extraction, for all the compounds, suggesting that the UAE method is more effective in the extraction of bioactive compounds. The extract obtained was used to make jelly and submitted to in vitro digestion and colonic fermentation. Loss of TP, TA and antioxidant activity in the jelly with addition of extract was lower when compared to extract loss itself, showing that it is feasible the hibiscus extract application in food products.

Kenaf (Hibiscus cannabinus L.) is an environmentally friendly natural fiber-producing plant that can produce diversified products, such as paper, wallcover, car interior, geotextile, soil safer, fiber drain, particle board, and plastic reinforcement as well as biofuel industry raw materials. Kenaf plant is a plant that has been only part of the fibers of the stem alone so that many unprofitable side products such as kenaf leaves after harvesting only as a waste. In Africa the leaves of kenaf are widely used as medicine This study aims to analyze phytochemical compounds and study the antioxidant activity of the kenaf leaf. The study started by doing phytochemical test and analyzing antioxidant in kenaf leaf and then testing its antioxidant activity with DPPH method. The results showed that the leaves of kenaf contain secondary metabolites of phytochemical compounds such as flavonoids, alkaloids, steroids, saponins, carbohydrates and tannins and have antioxidant activity that is quite good that is 44.4813 ppm.

This study evaluated the antidiabetic potentials of flavonoid-rich aqueous fraction of methanolic extract of Hibiscus sabdariffa calyx (HSCE) on the pancreatic β-cells of experimental type I diabetic model rats. Type 1 diabetes mellitus was induced in Wistar rats by a single intraperitoneal injection of 80mg/kg b/w streptozotocin (STZ) dissolved in 0.1M citrate buffer (pH 6.3). The rats were divided into five groups (n=12) including normal control group, test group I, diabetic negative control, test group II, and diabetic positive control. The test groups received 1.75g/kg b/w of HSCE by gavage for 15 days. Animals were sacrificed; the splenic portion of their pancreas and serum were evaluated for histopathological and biochemical parameters respectively. The regenerative effects of the extract on STZ-diabetes β-cells damage was evident from the results of the histopathological analysis and the biochemical parameters evaluated in the serum. Reduced levels of glutathione, catalase and superoxide dismutase in the serum of diabetic rats were significantly improved in the H. sabdariffa-treated rats (P0.05). Histological examination of pancreatic islet sections revealed degenerative and necrotic changes (D) in the pancreatic islet of Langerhans, β-cell degranulation, pyknotic β-cell nuclei, decreased islet cellular density, and severe vacuolation (V) in the islet of STZ-diabetic negative control group. The morphology of the pancreas of HSCE-treated diabetic rats (test group II) revealed remarkable improvements in the islet of Langerhans. Stereological studies also revealed that HSCE-treatment remarkably improved volume of the pancreatic islets and the numerical density of β-cell (number of β-cells per unit area of islet) depleted by STZ diabetes. The study concluded that possible antidiabetic mechanism of Hibiscus sabdariffa in STZ diabetes is through induction of β-cell regeneration and its strong antioxidant potential.

Immunomodulator is a compound that can increaase the imuno system. One of the plants that have immunomodulator’s activity is Waru Leaf (Hibiscus tiliaceus). the purpose of this research was to test the effect of immunomodulator by extract of Waru Leaf ethanol on rat male. The activity of immunomodulator was determined by using digital pletysmometer by measuring the differences between the last leg swelling’s volume and the first leg swelling’s volume. The treatment group were divided into 5 groups. Each group consistof 5 rats CMC-Na 0,5% (negative control), Stimuno® 32,5 mg/kgBW (positive control), dose of EEDW 50, 100 and 200 mg/kgBW, and bacteria E.coli as antigen. The results slowed that distribution of EEDW dose 200 mg/kgBW can give the effect of immunostimulant by swelling enthancement compared by CMC-Na 0,5 %. EEDW 200 mg/kgBW that have activity comparable with Stimuno® 32,5 mg/kgBW. Thus, it is concluded that of Waru Leaf extract has immunomodulator effects on delayed-type hypersensitivity response of rat male.

Context: Parkinson’s disease (PD) is a neurodegenerative disorder due to gradual loss of dopaminergic nerves in the substantia nigra (SN) in the midbrain. PD leads to certain motor disorders including resting tremor, muscle stiffness and slow movement. Medicinal plants have shown positive pharmacological effects in treating different models of PD. Objective: Tendency to use natural products, especially plants, for the treatment of PD has been growing. This article reviews the basic aspects of medicinal plants and their bioactive compounds that could be used to treat PD. Methods: Reliable articles indexed in databases ISI, SID, PubMed, PubMed Central, Scopus and Web of Science were used. A total of 12 plant-derived active ingredients and 18 herbal extracts were included. Different compounds have so far been isolated from plants that affect PD especially by targeting pathways associated with the pathogenesis of the disease. Results: Although some herbal extracts such as Hibiscus asper Hook. f. (Malvaceae), Ginkgo biloba L. (Ginkgoaceae), Carthamus tinctorius L (Asteraceae) and certain active ingredients, such as berberine and curcumin, have shown positive effects in animal models of PD, potential active ingredients and mechanisms of action should be investigated in additional studies. Discussion and conclusions: Despite the wide variety of plants in the world, a limited number of them have been studied for anti-Parkinsonian activity, and therefore, there are numerous perspectives in this field for future studies on plants and their bioactive compounds.

Background Current therapeutic approaches to treat metastatic breast cancer, although effective, have shown many inadvertent side effects such as genotoxicity due to a lack of selectivity. Thus, these treatment plans are not suitable for long-term usage. Natural health product extracts are safe for long-term consumption and some have shown to be medicinally active containing multiple bioactive compounds able target multiple vulnerabilities in cancer. One of which, Hibiscus rosa-sinesis (hibiscus) extract, has been reported to have many medicinal and anticancer properties due to its antioxidant and hypolipidemic effects. However, its efficacy against breast cancer has not been fully investigated and characterized. If effective against cancer, hibiscus extract could potentially be combined with chemotherapeutic treatments in adjuvant therapy to reduce chemotherapy-inducing side effects. Method We have investigated aqueous hibiscus flower extract anticancer efficacy, selectivity, and interactions with chemotherapeutics taxol, cisplatin, and tamoxifen in estrogen-receptor positive breast cancer cells, triple-negative human breast cancer cells, and normal non-cancerous cells. Apoptotic morphology and biochemical marker expression were assessed to determine the extent anticancer efficacy of hibiscus. Mitochondrial membrane potential reduction and reactive oxygen species generation were quantified using fluorogenic dyes to determine the mechanism of hibiscus extract action. Results Hibiscus extract was able to selectively induce apoptosis in both triple-negative and estrogen-receptor positive breast cancer cells in a dosage-dependent manner. Most importantly, addition of hibiscus extract was found to enhance the induction of apoptosis of chemotherapy treatments (taxol and cisplatin) in triple-negative breast cancer cells when compared to treatment alone. Moreover, hibiscus extract addition to chemotherapy treatment was able to increase oxidative stress and decrease mitochondrial membrane potential compared to individual treatments. Conclusion Hibiscus extract is effective on breast cancer, most notably on generally resistant triple-negative breast cancer, while being selective for normal healthy cells. Hibiscus extract could supplement chemotherapeutic regimens as an adjuvant and lead to a more efficacious treatment approach to reduce chemotherapy dosages and related toxicity.

Hibiscus sabdariffa L. calyces are underutilized sources of health-promoting anthocyanins. Infusions are the most common way to consume them, but because anthocyanins are thermosensitive, prolonged extraction times at high temperatures may reduce their bioactivities, suggesting the need to identify optimal preparation conditions. Response surface methodology was used to establish calyces-to-water ratio (X1: 1–20 g/100 mL), temperature (X2: 70–100 °C), and time (X3: 1–30 min) that would produce an infusion with optimized total anthocyanin content (TAC) and antioxidant activity. Under optimum conditions (X1=10 g/100 mL, X2=88.7 °C, and X3=15.5 min) TAC was 132.7±7.8 mg cyanidin-3-glucoside equivalents (C3G)/100 mL, and antioxidant activity was 800.6±69.9 (DPPH assay), and 1792.0±153.5 (ABTS assay) μmol Trolox equivalents (TE)/100 mL. Predicted and experimental results were statistically similar. Identifying ideal processing conditions can promote consumption of an H. sabdariffa-based functional beverage with high anthocyanin content and antioxidant activity that exert health-promoting bioactivities on the consumer.

Breast cancer (MCF-7), as well as liver cancer (HepG2), were the leader among newcancer cases. The objective of this study is to assess the viability percentage of tow traditional drinks(hibiscus and liquorice extracts) toward human breast and liver cancer cell lines at differentconcentrates from 250 to 5000 μg/ml in hibiscus extract and from 50 to 3000 μg/ml in liquoriceextract. The viability percentage of hibiscus on breast and liver cancer cell lines were 86.9% and84.7% at 5000 μg/ml, respectively. The IC50 of the hibiscus extract were 18478.6 μg/ml and 16159μg/ml breast and liver cancer. In addition, the viability percentage of liquorice extract on breast andliver cancer cell lines were determined. Results revealed that liquorice extract has possessed high anticarcinogenicproperties it was zero (%) at 3000 μg/ml for breast cancer and 5% for liver cancer atsame concentration, the IC50 was 491.7 μg/ml in breast cancer and was 125 μg/ml in liver cancer cellline. DNA damage was determined to detect oxidative DNA damage in HepG2 cell line. This enhances therole of these traditional drinks especially liquorice as anti-cancer agents.

Hibiscus rosa-sinensis Linn. merupakan salah satu tanaman obat di Indonesia yang digunakan sebagai kontrasepsi pria. Bunga kembang sepatu mengandung flavonoid yang dapat menurunkan testosteron. Tujuan penelitian ini untuk mengetahui pengaruh ekstrak bunga kembang sepatu terhadap berat epididimis, tebal epitel epididimis cauda, berat prostat dan vesikula seminalis tikus jantan. Penelitian ini merupakan penelitian eksperimental dengan menggunakan Rancangan Acak Lengkap (RAL). Sampel dalam penelitian ini menggunakan 25 tikus jantan strain Sprague Dawley® usia 60-70 hari dengan berat badan 200-210 gram. Dosis perlakuan 200 mg/kgBB, 300 mg/kgBB, 400 mg/kgBB, 500 mg/kgBB selama 30 hari. Data disajikan dalam bentuk tabel serta narasi untuk menginterpretasikan data tersebut.Hasil penelitian menunjukan adanya penurunan berat epididimis pada kelompok perlakuan dibanding kelompok kontrol (p=0,000), terjadi juga penyusutan tebal epitel epididimis cauda (p=0,021), penurunan berat prostat dan vesikula seminalis antara kelompok perlakuan dibanding kelompok kontrol (p=0,001). Dapat disimpulkan bahwa ada pengaruh ekstrak bunga kembang sepatu tehadap epididimis, prostat dan vesikula seminalis. Pengaruh tersebut kemungkinan disebabkan kandungan bahan kimia yang ada di dalam bunga kembang sepatu yang bersifat antifertilitas.