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Ethnopharmacological relevance Hypertension is an important public health challenge in low- and middle-income countries, and in many African countries including Guinea medicinal plants are still widely used for its treatment. Materials and Methods The objective of this study was to determine the prevalence of hypertension in two Guinean urban districts (Pounthioun and Dowsare), to describe its management and to collect information on traditional herbal remedies. A total of 316 participants entered the study, 28.2% (89/316) men and 71.8% (227/316) women. Of these, 181 were from Dowsare (50 men and 131 women) and 135 from Pounthioun (39 men and 96 women). The mean age of subjects was 40.8 ± 14.0 years (range18 − 88years), while the majority of subjects (63.3% or 200/316) were 45–74 years old. Results The overall prevalence of hypertension was 44.9% (142/316): 46.4% (84/181) from Dowsare and 43.0% (58/135) from Pounthioun. Ethnobotanical investigations among hypertensive patients led to the collection of 15 plant species, among which Hymenocardia acida leaves and Uapaca togoensis stem bark were the most cited. Phytochemical investigation of these two plant species led to the isolation and identification of isovitexin and isoorientin from H. acida, and betulinic acid and lupeol from U. togoensis. Conclusion The presence of these constituents in Hymenocardia acida leaves and Uapaca togoensis stem bark may at least in part support their traditional use against hypertension in Guinea.

Ethnopharmacological relevance Terminalia albida (Combretaceae), widely used in Guinean traditional medicine, showed promising activity against Plasmodium falciparum and Candida albicans in previous studies. Bioassay-guided fractionation was carried out in order to isolate the compounds responsible for these activities. Materials and methods Fractionation and isolation were performed by flash chromatography, followed by semi-preparative HPLC-DAD-MS. The structural elucidation of the isolated compounds was carried out by 1D and 2D NMR as well as HR-ESI-MS. Isolated compounds were evaluated against Plasmodium falciparum, Candida albicans, Staphylococcus aureus and Escherichia coli, and their cytotoxicity against MRC-5 cells was determined. Results Bioassay-guided fractionation of Terminalia albida root resulted in the isolation of 14 compounds (1–14), and their antimicrobial properties were evaluated. Pantolactone (1) (IC50 0.60 ± 0.03 μM) demonstrated significant activity against P. falciparum. Other compounds, including 3,4,3′-tri-O-methyl-ellagic acid (3), the triterpenes arjunolic acid (5), arjungenin (6), arjunic acid (7) and arjunglucoside II (10), and the phenol glycoside calophymembranside-B (14), were less active and showed IC50 values in the range 5–15 μM. None of the tested compound showed antibacterial or antifungal activity. Conclusion These results may explain at least in part the activity of the root extract of T. albida against P. falciparum.

Ethnopharmacological relevance In sub-Saharan Africa, concomitant occurrence of malaria and invasive infections with micro-organisms such as Gram-positive Staphylococcus aureus , Gram-negative Escherichia coli and yeasts or fungi such as Candida albicans and Aspergillus fumigatus is common. Non-tuberculous mycobacteriosis caused by Mycobacterium chelonae has been recognized as a pulmonary pathogen with increasing frequency without effective therapy. Although less important, the high incidence of Trichophyton rubrum infections along with its ability to evade host defense mechanisms, accounts for the high prevalence of infections with this dermatophyte. Considering the treatment cost of both malaria and microbial infections, along with the level of poverty, most affected African countries are unable to cope with the burden of these diseases. In sub-Saharan Africa, many plant species are widely used in the treatment of these diseases which are traditionally diagnosed through the common symptom of fever. Therefore it is of interest to evaluate the antimicrobial activities of medicinal plants reported for their use against malaria/fever. Materials and methods Based on an ethnobotanical survey, 34 Guinean plant species widely used in the traditional treatment of fever and/or malaria have been collected and evaluated for their antimicrobial activities. Plants extracts were tested against Candida albicans , Trichophyton rubrum , Aspergillus fumigatus , Mycobacterium chelonae , Staphylococcus aureus and Escherichia coli . Results The most interesting activities against Candida albicans were obtained for the polar extracts of Pseudospondias microcarpa and Ximenia americana with IC 50 values of 6.99 and 8.12 µg/ml, respectively. The most pronounced activity against Trichophyton rubrum was obtained for the ethanol extract of Terminalia macroptera (IC 50 5.59 µg/ml). Only 7 of the 51 tested extracts were active against Staphylococcus aureus . From these, the methanolic extracts of the leaves and stem bark of Alchornea cordifolia were the most active with IC 50 values of 2.81 and 7.47 µg/ml, respectively. Only Terminalia albida and Lawsonia inermis showed activity against Mycobacterium chelonae . None of the tested extracts was active against Escherichia coli . Conclusion A number of traditional Guinean plant species used against malaria/fever showed, in addition to their antiplasmodial properties and antimicrobial activity. The fact that some plant species are involved in the traditional treatment of malaria/fever without any antiplasmodial evidence may be justified by their antimicrobial activities.

Based on an ethnobotanical survey, 41 Guinean plant species widely used in the traditional treatment of fever and/or malaria were collected. From these, 74 polar and apolar extracts were prepared and tested for their in vitro antiprotozoal activity along with their cytotoxicity on MRC-5 cells. A potent activity (IC50 100. These results may validate at least in part the traditional use of some of the plant species.

Objectifs Determiner la prevalence de l'HTA en zone urbaine de Labe, evaluer l'efficacite des trois phytomedicaments, evaluer la tolerance des trois phytomedicaments. Methodes L'etude a ete realisee en deux phases : la premiere a consiste en une enquete de prevalence pour determiner les patients hypertendus ; la deuxieme a permis de soumettre les patients hypertendus (volontaires) a l'evaluation ethnotherapeutique. L'etude de prevalence a porte sur 316 patients âges de 18 ans et plus residents en zone urbaine de Labe. Elle a ete realisee sur base d'interwiew selon une fiche d'enquete standardisee par le Centre de Recherche et de Valorisation des Plantes Medicinales de Dubreka. Concernant l'evaluation ethnotherapeutique, l'accord du Comite National d'Ethique guineen a ete obtenu avant le demarrage. Les phytomedicaments ont ete prepares a partir des deux plantes : Uapaca togoensis et Hymenocardia acida. Resultats La prevalence de l'HTA en zone urbaine de Labe a ete estimee a 27,52 % (87/316) avec une predominance feminine (75,86 %). L'evaluation de l'efficacite et de la tolerance de 3 phytomedicaments a base de Uapaca togoensis et Hymenocardia acida a ete realisee. L'evaluation clinique a trois bras a permis de relever une baisse faible a modeste de la PAS et/ou de la PAD chez 37 patients soumis aux traitements soit a Hymenocardia acida, Uapaca togoensis ou a l'association Hymenocardia acida et Uapaca togoensis. A l'issue de 14 jours de traitement, le phytomedicament a base de Hymenocardia acida a entraine des baisses plus importantes que le phytomedicament a base de Uapaca togoensis et a l'association Hymenocardia acida et Uapaca togoensis. Conclusion Les baisses les plus importantes ont ete observees au niveau du groupe soumis au traitement par H. acida. La tolerance a ete jugee bonne pour les trois phytomedicaments