5 results on '"light chain restriction"'
Search Results
2. Ultrasensitive automated RNA in situ hybridization for kappa and lambda light chain mRNA detects B-cell clonality in tissue biopsies with performance comparable or superior to flow cytometry
- Author
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Xiao-Jun Ma, Sarah L. Ondrejka, Claudiu V. Cotta, Ling Guo, Siobhan Kernag, James R. Cook, Courtney Anderson, Emerald Doolittle, and Zhen Wang
- Subjects
0301 basic medicine ,Pathology ,medicine.medical_specialty ,Lymphoma, B-Cell ,Biopsy ,Context (language use) ,In situ hybridization ,Biology ,Immunoglobulin light chain ,Sensitivity and Specificity ,Article ,Pathology and Forensic Medicine ,Flow cytometry ,Immunoglobulin kappa-Chains ,03 medical and health sciences ,0302 clinical medicine ,Immunoglobulin lambda-Chains ,medicine ,Humans ,RNA, Messenger ,RNAscope ,In Situ Hybridization ,B-Lymphocytes ,Messenger RNA ,medicine.diagnostic_test ,B-cell lymphoma ,RNA ,Light chain restriction ,Flow Cytometry ,Immunohistochemistry ,Molecular biology ,Clone Cells ,Staining ,030104 developmental biology ,CISH ,030220 oncology & carcinogenesis ,RNA in situ hybridization - Abstract
The assessment of B-cell clonality is a critical component of the evaluation of suspected lymphoproliferative disorders, but analysis from formalin fixed paraffin embedded tissues can be challenging if fresh tissue is not available for flow cytometry. Immunohistochemical and conventional bright field in situ hybridization stains for kappa and lambda are effective for evaluation of plasma cells, but are often insufficiently sensitive to detect the much lower abundance of light chains present in B cells. We describe an ultrasensitive RNA in situ hybridization assay which has been adapted for use on an automated immunohistochemistry platform and compare results with flow cytometry in 203 consecutive tissues and 104 consecutive bone marrows. Overall, in 203 tissue biopsies, RNA in situ hybridization identified light chain restricted B-cells in 85 (42%) vs. 58 (29%) by flow cytometry. Within 83 B-cell non-Hodgkin lymphomas, RNA in situ hybridization identified a restricted B-cells in 74 (89%) vs. 56 (67%) by flow cytometry. B-cell clonality could be evaluated in only 23/104 (22%) bone marrow cases due to poor RNA preservation, but evaluable cases showed 91% concordance with flow cytometry. RNA in situ hybridization allowed for recognition of biclonal/composite lymphomas not identified by flow cytometry, and highlighted unexpected findings, such as coexpression of kappa and lambda RNA in 2 cases and the presence of lambda light chain RNA in a T lymphoblastic lymphoma. Automated RNA in situ hybridization showed excellent interobserver reproducibility for manual evaluation (average K=0.92), and an automated image analysis system showed high concordance (97%) with manual evaluation. Automated RNA in situ hybridization staining, which can be adopted on commonly utilized immunohistochemistry instruments, allows for the interpretation of clonality in the context of the morphologic features in formalin fixed, paraffin embedded tissues with a clinical sensitivity similar or superior to flow cytometry.
- Published
- 2018
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3. Localized accumulation of kappa restricted Russell body‐containing plasma cells in tonsil
- Author
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Katsiaryna Laziuk, Richard D. Hammer, and Maryna Vazmitsel
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Pathology ,medicine.medical_specialty ,Russell bodies ,lcsh:Medicine ,Case Report ,clonality ,Case Reports ,030204 cardiovascular system & hematology ,Plasma cell ,Immunoglobulin light chain ,plasma cells ,03 medical and health sciences ,0302 clinical medicine ,light chain restriction ,Medicine ,lcsh:R5-920 ,Adult patients ,business.industry ,Russell body ,lcsh:R ,Germinal center ,General Medicine ,medicine.anatomical_structure ,tonsil ,030220 oncology & carcinogenesis ,Tonsil ,lcsh:Medicine (General) ,business ,Kappa - Abstract
An abnormal clonal plasma cell proliferation with Russell bodies is rare in chronic inflammatory reactions in adult patients. We describe the first case of light chain restricted Russell body accumulation within germinal centers of lymphoid follicles of the tonsil in a child. This should not be confused with a neoplastic process.
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- 2019
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4. Technique for single-step lymphocyte isolation from an endoscopic biopsy specimen for the diagnosis of gastrointestinal lymphoma
- Author
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Daisuke Ennishi, Katsunori Matsueda, Tadashi Yoshino, Fumio Otsuka, Takehiro Tanaka, Masaya Iwamuro, Sizuma Omote, Takahide Takahashi, Natsuki Watanabe, and Hiroyuki Okada
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Lymphocyte ,Clinical Biochemistry ,Single step ,Gastrointestinal mucosa ,010501 environmental sciences ,01 natural sciences ,Flow cytometry ,03 medical and health sciences ,medicine ,lcsh:Science ,030304 developmental biology ,0105 earth and related environmental sciences ,0303 health sciences ,medicine.diagnostic_test ,business.industry ,Endoscopic biopsy ,Method Article ,Light chain restriction ,medicine.disease ,Isolation (microbiology) ,Gastrointestinal lymphoma ,Lymphoma ,Medical Laboratory Technology ,medicine.anatomical_structure ,Lymphocyte isolation ,lcsh:Q ,business ,Nuclear medicine - Abstract
In this paper, we introduce a simplified, one-step procedure for lymphocyte isolation from an endoscopically biopsied fragment. For lymphocyte isolation, an endoscopically harvested specimen and 5 mL of normal saline solution were placed in a wire mesh strainer set in a porcelain bowl. To obtain the lymphocyte suspension, the solid specimen was crushed using the rubber portion of a plunger of a 10 mL injection syringe. Flow cytometry was performed using the lymphocyte suspension. For validating our methods, the one-step lymphocyte isolation technique was used to perform flow cytometry on samples from 23 patients with (n = 12) or without (n = 11) gastrointestinal lymphoma. Flow cytometry of light chain expression was performed in all patient samples (feasibility: 100%). Sensitivity was 83.3% (10/12) and specificity was 100% (11/11). In conclusion, lymphocytes isolated from a single endoscopic biopsy specimen using our simplified and quick procedure are suitable for flow cytometry. Considering that flow cytometry has an important advantage of providing the results on the examination day itself, the results of this study suggest that flow cytometric analysis using our single-step lymphocyte isolation technique can be potentially used to diagnose lymphoma in the gastrointestinal mucosa. • We introduce a simplified, one-step procedure for lymphocyte isolation from an endoscopically biopsied fragment. • Our technique is feasible for flow cytometric analysis in patients with gastrointestinal lymphoma as well as those with gastrointestinal lesions that are suspected to be lymphoma., Graphical Abstract Image, graphical abstract
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- 2020
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5. Lymphoblastic leukemia with surface light chain restriction: A diagnostic dilemma
- Author
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Kiran Ghodke, Nikhil Rabade, Sumeet Gujral, Pratibha Amare, Nikhil Patkar, Asma Bibi, Prashant Tembhare, and P.G. Subramanian
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Microbiology (medical) ,Pathology ,medicine.medical_specialty ,Lymphoblastic Leukemia ,leukemia ,lcsh:QR1-502 ,General Medicine ,Diagnostic dilemma ,Biology ,medicine.disease ,Immunoglobulin light chain ,Molecular biology ,lcsh:Microbiology ,Precursor B-Cell Lymphoblastic Leukemia-Lymphoma ,Pathology and Forensic Medicine ,Leukemia ,Immunophenotyping ,light chain restriction ,hemic and lymphatic diseases ,lcsh:Pathology ,medicine ,Neoplasm ,Burkitt ,B Acute Lymphoblastic Leukemia ,lcsh:RB1-214 - Abstract
Surface light chain expression is a feature of mature B-cell neoplasms. Light chain restriction in precursor B acute lymphoblastic leukemia is infrequently seen. We report a case of a 28-year-old female with non-FAB L3 morphology blasts and immunophenotypic features showing overlap between a precursor and mature B-cell neoplasm.
- Published
- 2016
- Full Text
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