10 results on '"Yoshikazu Harada"'
Search Results
2. Strong expression of polypeptide N-acetylgalactosaminyltransferase 3 independently predicts shortened disease-free survival in patients with early stage oral squamous cell carcinoma
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Kimitoshi Kohno, Yasuyuki Sasaguri, Toshiyuki Nakayama, Yoshikazu Harada, Hirotsugu Noguchi, Yuichiro Kawatsu, Hiroshi Hijioka, Hidetaka Uramoto, Akihiko Miyawaki, Tomoko Kimura, Sohsuke Yamada, Hiroto Izumi, Akihiro Kuma, Ryoichi Oya, Shohei Kitada, and Ke-Yong Wang
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Male ,Oncology ,Cytoplasm ,Cancer Research ,Pathology ,Glycosylation ,Metastasis ,Pathogenesis ,Neoplasm Metastasis ,Stage (cooking) ,Aged, 80 and over ,Mouth neoplasm ,Cell Differentiation ,General Medicine ,Middle Aged ,Disease-free survival (DFS) ,Prognosis ,Immunohistochemistry ,Neoplasm Proteins ,Gene Expression Regulation, Neoplastic ,Lymphatic system ,Carcinoma, Squamous Cell ,Disease Progression ,N-Acetylgalactosaminyltransferases ,Female ,Mouth Neoplasms ,Original Article ,Erratum ,Adult ,medicine.medical_specialty ,Biology ,Polypeptide N-acetylgalactosaminyltransferase 3 (GalNAc-T3) ,Disease-Free Survival ,Young Adult ,Cell Line, Tumor ,Internal medicine ,Biomarkers, Tumor ,Cell Adhesion ,medicine ,Humans ,Neoplasm Invasiveness ,Aged ,Vessel invasion ,Gene Expression Profiling ,Early stage OSCC (ESOSCC) ,medicine.disease ,Oral squamous cell carcinoma (OSCC) ,carbohydrates (lipids) ,Microscopy, Fluorescence ,Tumor progression ,Cancer cell ,Neoplasm Recurrence, Local - Abstract
The polypeptide N-acetylgalactosaminyltransferase (GalNAc-Ts) family of enzymes regulates the critical initial steps of mucin-type O-glycosylation. Among GalNAc-Ts that may significantly influence cancer biology, thus affecting cell differentiation, adhesion, invasion, and/or metastasis, GalNAc-T3 exhibits a high expression in several human cancers, closely associated with tumor progression and a poor prognosis. However, the expression pattern of GalNAc-T3 in oral squamous cell carcinoma (OSCC) remains obscure. Since postoperative recurrence of even early stage OSCC (ESOSCC) occurs at an early phase, significantly affecting their clinical course and worse outcome, the identification of clinically significant accurate biomarkers is needed. Therefore, we investigated the correlation between the immunohistochemical GalNAc-T3 expression and various clinicopathological characteristics and recurrence using 110 paraffin-embedded tumor samples obtained from patients with surgically resected ESOSCC (T1–2N0). Recurrence was recognized in 37 of 110 (33.6 %) patients. The GalNAc-T3 expression was considered to be strongly positive when 20 % or more of the cancer cells showed positive cytoplasmic staining. Consequently, a strong expression of GalNAc-T3 was observed in 40 patients (36.4 %), showing a close relationship to poor differentiation, the presence of lymphatic and vascular invasion, and recurrence. Univariate and multivariate analyses further demonstrated that the patients with a strong GalNAc-T3+ status had markedly lower disease-free survival (DFS) rates, especially within the first 2 years postoperatively. Therefore, GalNAc-T3 might play a role in the pathogenesis of ESOSCC recurrence, and its immunohistochemical detection potentially predicts a shorter DFS and may be a useful parameter for providing clinical management against ESOSCC in the early postoperative phase. Electronic supplementary material The online version of this article (doi:10.1007/s13277-015-3928-7) contains supplementary material, which is available to authorized users.
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- 2015
3. A case of osteoid osteoma in the mandible with increasing size and intensifying pain
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Yoshikazu Harada, Soichi Hirashima, Toshiyuki Akimori, Akihiko Miyawaki, Ryoichi Oya, and Satomi Otsu
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Orthodontics ,Osteoid osteoma ,business.industry ,Mandible ,Medicine ,business ,medicine.disease - Published
- 2014
4. Expression of vascular endothelial growth factor and its receptor KDR (kinase domain-containing receptor)/Flk-1 (fetal liver kinase-1) as prognostic factors in human colorectal cancer
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Yoshikazu Harada, Yutaka Ogata, and Kazuo Shirouzu
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Vascular Endothelial Growth Factor A ,Colorectal cancer ,Endothelial Growth Factors ,Adenocarcinoma ,Vascular endothelial growth inhibitor ,Metastasis ,chemistry.chemical_compound ,medicine ,Humans ,Receptors, Growth Factor ,Growth factor receptor inhibitor ,Neoplasm Staging ,Retrospective Studies ,Lymphokines ,Vascular Endothelial Growth Factors ,business.industry ,Microcirculation ,Receptor Protein-Tyrosine Kinases ,Hematology ,General Medicine ,Prognosis ,medicine.disease ,Neoplasm Proteins ,Vascular endothelial growth factor ,Vascular endothelial growth factor A ,Receptors, Vascular Endothelial Growth Factor ,Oncology ,Vascular endothelial growth factor C ,chemistry ,Cancer research ,Surgery ,Neoplasm Recurrence, Local ,Colorectal Neoplasms ,business ,Tyrosine kinase ,Follow-Up Studies - Abstract
Background. To clarify the clinical significance of the expression of vascular endothelial growth factor (VEGF) and its receptor, kinase domain-containing receptor (KDR) in colorectal cancer, we evaluated the relationship between the expression of VEGF and KDR, and the microvessel counts and clinicopathological factors in colorectal cancer. Methods. A total of 259 specimens from sequential colorectal cancer patients who had undergone surgery were examined by the avidin-biotin peroxidase complex method, using anti-human VEGF, anti-human KDR, and anti-human von Willebrand factor antibodies. Results. The incidence of VEGF expression in the tumor cells of the patients with liver metastasis was significantly higher than that in the tumor cells of the patients without liver metastasis (67% vs 44%). The microvessel count at the tumor invasive edge in the patients whose tumor cells were positive for VEGF was significantly higher than that in the patients whose tumor cells were negative for VEGF (33.0 ± 7.8 vs 28.0 ± 7.9); the significant difference in microvessel counts was greater when there was a combination of VEGF and KDR expression. The overall survival rate of patients positive for VEGF was significantly (P = 0.0276) lower than that of those who were negative for VEGF. Although there was no significant difference (P = 0.0743) in the survival rates after potentially curative resection according to VEGF expression, the survival rate of the patients positive for both VEGF in tumor cells and KDR in endothelial cells was significantly (P = 0.0026) lower than that in the patients who were negative for VEGF and/or KDR. In addition, multivariate analysis revealed that the expression of both VEGF and KDR was an independent prognostic factor even after potentially curative resection. Conclusion. VEGF may be implicated in the definition of the malignant phenotype of colorectal cancer via tumor angiogenesis. VEGF and its receptor KDR expression in tumorous tissues could be useful prognostic factors in colorectal cancer.
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- 2001
5. Effect of methionine-free total parenteral nutrition and insulin-like growth factor I on tumor growth in rats
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Shogo Yoshida, Junji Ohta, Kazuo Shirouzu, Yoshikazu Harada, Hideaki Yamana, Yuichirou Shirouzu, and Nobuya Ishibashi
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Male ,medicine.medical_specialty ,Physiology ,Endocrinology, Diabetes and Metabolism ,medicine.medical_treatment ,Endogeny ,Biology ,Receptor, IGF Type 1 ,Cachexia ,chemistry.chemical_compound ,Insulin-like growth factor ,Liver Neoplasms, Experimental ,Methionine ,Leucine ,Weight loss ,Physiology (medical) ,Internal medicine ,Weight Loss ,medicine ,Animals ,Insulin-Like Growth Factor I ,Muscle, Skeletal ,Growth factor ,Body Weight ,Proteins ,Rats, Inbred Strains ,Organ Size ,medicine.disease ,Neoplasm Proteins ,Rats ,Protein catabolism ,Endocrinology ,Parenteral nutrition ,Liver ,chemistry ,Protein Biosynthesis ,Parenteral Nutrition, Total ,medicine.symptom - Abstract
The objectives of this study were to evaluate the effect of insulin-like growth factor I (IGF-I) on the fractional synthesis rate (FSR) of muscle and whole body protein breakdown rate (WPBR) during methionine-free total parenteral nutrition (MTPN). We also determined whether the inhibition of endogenous methionine availability reduced tumor protein synthesis. AH109A hepatoma cells were inoculated onto the backs of Donryu rats on day 0. On day 10, the rats were catheterized for TPN and assigned to one of four groups: 1) standard TPN (STPN), 2) STPN + IGF-I, 3) MTPN, or 4) MTPN + IGF-I. The addition of IGF-I to MTPN reduced the loss of body weight by both increasing muscle FSR and reducing WPBR. The tumor FSR did not differ between MTPN + IGF-I and MTPN. The methionine extraction ratio from the liver was negative with MTPN + IGF-I but positive in the other groups. We concluded that IGF-I blockage of endogenous methionine release from peripheral protein sites was associated with a shift to liver-derived methionine, with no change in tumor growth in MTPN-treated rats.
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- 1997
6. Expression and Localization of Vascular Endothelial Growth Factor (VEGF) in Colorectal Cancer
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Yutaka Ogata, Kiyohisa Inuzuka, Y. Hara, Mamoru Hiraki, H. Oda, K. Shirouzu, Kenji Yamauchi, Yoshikazu Harada, Yoshito Akagi, and J. Konishi
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biology ,business.industry ,Colorectal cancer ,VEGF receptors ,Gastroenterology ,Vascular endothelial growth inhibitor ,medicine.disease ,Vascular endothelial growth factor B ,Vascular endothelial growth factor ,Vascular endothelial growth factor A ,chemistry.chemical_compound ,chemistry ,Vascular endothelial growth factor C ,Neuropilin 1 ,Cancer research ,biology.protein ,Medicine ,Surgery ,business - Published
- 1997
7. Immunohistochemical Localization of Vascular Endothelial Growth Factor in Esophageal Cancer
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Yoshikazu Harada, Teruhiko Fujii, Hideaki Yamana, Kazuo Shirouzu, Yutaka Ogata, and Hiromasa Fujita
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Vascular Endothelial Growth Factor A ,Pathology ,medicine.medical_specialty ,Esophageal Neoplasms ,Angiogenesis ,Endothelial Growth Factors ,chemistry.chemical_compound ,Humans ,Medicine ,Lymphokines ,biology ,Vascular Endothelial Growth Factors ,business.industry ,General Medicine ,Esophageal cancer ,medicine.disease ,Immunohistochemistry ,Squamous carcinoma ,Vascular endothelial growth factor ,chemistry ,Tumor progression ,Cytoplasm ,biology.protein ,business ,Peroxidase - Abstract
We have studied the expression of vascular endothelial growth factor (VEGF) in esophageal cancer using immunohistochemistry. A total of 101 specimens of esophageal cancer tissue were fixed by formalin, embedded in paraffin wax, and examined in 3μm sections by avidin-biotin peroxidase complex method. VEGF was noted in the cytoplasm of normal esophageal glandular cells, monocyte-macrophages, squamous carcinoma cells and of the vascular endothelial cells themselves. VEGF expression by monocyte-macrophages was observed in all cases, in contrast the incidence of VEGF expression in the tumor cells was relatively low at 26.7% of all specimens. However, in the cases where the tumor cells were positive for VEGF, it was discovered that the main source of the VEGF production was the tumor cells themselves. In the cases with proper mucosal invasion the incidence of VEGF expression by the tumor cells was quite low at 7.6%. However, when the tumor invaded the submucosal layer the expression increased to 33.3%. There was also a significant correlation in those with the submucosal invasion between the expression of VEGF in the tumor cells and the density of microvessels at the tumor invasive edge. These results suggest that VEGF may play an important role in tumor progression and in the angiogenesis via auto-crine and para-crine mechanisms in esophageal cancer.
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- 1996
8. A Case Report of Rectal Schwannoma
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Yoshikazu Harada, Kohta Yatsuka, Yuichiro Shirouzu, Kazuo Shirouzu, Atsushi Jimi, Seiya Ishibashi, Naotaka Murakami, Yasuhiro Ohmori, and Takaho Tanaka
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Male ,medicine.medical_specialty ,Rectal Neoplasms ,business.industry ,Submucosal tumor ,General surgery ,Rectum ,General Medicine ,Schwannoma ,medicine.disease ,medicine.anatomical_structure ,Rare case ,otorhinolaryngologic diseases ,medicine ,Humans ,business ,Neurilemmoma ,Aged - Abstract
We experienced a case of rectal schwannoma which was diagnosed before surgery. In this paper we have described this rare case and have discussed a number of similar cases reported in the literature.
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- 1996
9. A CASE OF MALIGNANT LYMPHOMA OF CHEST WALL COMBINED WITH CHRONIC EMPYEMATHORAX
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Yasuhiro Ohmori, Atsuroh Jimi, Nobuya Ishibashi, Takaho Tanaka, Yoshikazu Harada, Yuhichiroh Shirohzu, Teruo Kakegawa, Nobuharu Uchida, and Kohta Yatsuka
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Thorax ,medicine.medical_specialty ,Chemotherapy ,Rib cage ,Pathology ,medicine.diagnostic_test ,Parietal Pleura ,business.industry ,medicine.medical_treatment ,medicine.disease ,Asymptomatic ,Metastasis ,Biopsy ,medicine ,Radiology ,Radical surgery ,medicine.symptom ,business - Abstract
It has increased that we encounter reports concerning a malignant lymphoma which appeared in the chest wall several decades after tuberculous empyemathorax or tuberculous pleuritis. A 54-year-old male, with asymptomatic chronic empyema thorax was admitted to the hospital because of pain from a huge chest tumor. The chest CT and MRI studies revealed that the tumor of the chest wall invaded the ribs, and it seems to arise from the chest wall which adjoined to the peel of the parietal pleura with severe carcifications of the empyemathorax. Biopsy of the tumor revealed a non-epitherial malignant tumor but correct diagnosis of a malignant lymphoma could not be attained. The tumor of the chest wall was widely resected and a radical surgery was also performed for chronic empyemathorax. The resected specimens were pathologically diagnosed as non-Hodgikin's malignant lymphoma which was of B cell lymphocyte origin, diffuse type and medium sized cell type by LSG classification. Two courses of chemotherapy with ACOP were administered after the surgery. There has been no sign of local recurrences and metastasis up to now. We discussed and reviewed this unusual cases which have been previously published in the Japanese literature.
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- 1994
10. Embryonal rhabdomyosarcoma arising from the uterine corpus in a postmenopausal female: a surgical case challenging the genuine diagnosis on a cytology specimen
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Yoshikazu Harada, Hirotsugu Noguchi, Toshiyuki Nakayama, Akihide Tanimoto, Satoshi Kimura, Naoko Satoh, and Sohsuke Yamada
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0301 basic medicine ,Pathology ,medicine.medical_specialty ,Histology ,Biopsy ,Case Report ,Biology ,Pathology and Forensic Medicine ,Diagnosis, Differential ,03 medical and health sciences ,0302 clinical medicine ,Uterine corpus ,Predictive Value of Tests ,Cytology ,Biomarkers, Tumor ,medicine ,Humans ,Rhabdomyosarcoma, Embryonal ,Rhabdomyosarcoma ,Uterine Neoplasm ,medicine.diagnostic_test ,General Medicine ,Anatomy ,Middle Aged ,medicine.disease ,Immunohistochemistry ,Magnetic Resonance Imaging ,Abdominal mass ,Tumor Burden ,Postmenopause ,Treatment Outcome ,030104 developmental biology ,030220 oncology & carcinogenesis ,Uterine Neoplasms ,Female ,Enlarged Uterus ,Embryonal rhabdomyosarcoma ,Sarcoma ,medicine.symptom - Abstract
A 55-year-old postmenopausal female presented with genital bleeding and lower abdominal mass. An abdominal MRI revealed a heterogeneously enhanced, 15 × 10 cm mass, completely filling the lumen of the enlarged uterus. The cytologic analysis of the mass showed tumor cells in small clusters and as individual cells showing hyperchromatic round to oval nuclei, and pleomorphic and occasionally unipolar "tadpole"-shaped cytoplasm, in a background of severe necrosis and many degenerated squamous cells. We first interpreted it merely as atypical cells, possibly originated from sarcoma. A total abdominal hysterectomy and salpingo-oophorectomy were performed, and gross examination showed an exophytic polypoid mass with a whitish to white-grayish, necrotic appearance, protruding from the endometrial mucosa. Microscopically, the tumor was composed of a diffuse proliferation of highly atypical spindle-shaped cells, admixed with many characteristic rhabdomyoblasts having abundant densely eosinophilic cytoplasm with sometimes distinct cross-striations, coexisted with cellular primitive small blue round to oval cells foci. However, neither carcinoma nor additional heterologous sarcoma components were completely seen within our thorough investigation. Therefore, we finally made a diagnosis of embryonal rhabdomyosarcoma arising from the uterine corpus. We should be aware that owing to its characteristic features, cytopathologists might be able to determine a genuine diagnosis, based on multiple and adequate cytology samplings.
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