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Start Over Author "Trofimova ES" Topic mesenchymal stem cells
6 results on '"Trofimova ES"'

1. Role of JNK and Contribution of p53 into Growth Potential of Mesenchymal Progenitor Cells In Vitro.

Author

Zyuz'kov GN, Zhdanov VV, Udut EV, Miroshnichenko LA, Danilets MG, Simanina EV, Trofimova ES, Chaikovskii AV, Agafonov VI, Sherstoboev EY, Minakova MY, Burmina YV, Udut VV, and Dygai AM

Subjects
Animals, Cell Proliferation drug effects, Hydroxyurea, In Vitro Techniques, Male, Mesenchymal Stem Cells metabolism, Mice, Mice, Inbred CBA, Statistics, Nonparametric, Toluene pharmacology, Benzothiazoles pharmacology, Cell Proliferation physiology, MAP Kinase Kinase 4 antagonists & inhibitors, Mesenchymal Stem Cells physiology, Protein Kinase Inhibitors pharmacology, Signal Transduction physiology, Toluene analogs & derivatives, Tumor Suppressor Protein p53 antagonists & inhibitors
Abstract

Specific JNK and p53 inhibitors stimulated the formation of fibroblast colonies (CFU-F) and clusters (ClFU-F) and increased proliferative activity of mesenchymal progenitor cells. No effects of inhibitors of JNK and p53 on differentiation of progenitor elements were revealed.

Published
2015
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2. PI3K, MAPK EPK1/2 and p38 are involved in the realization of growth potential of mesenchymal progenitor cells under the influence of basic fibroblast growth factor.

Author

Zyuz'kov GN, Danilets MG, Ligacheva AA, Zhdanov VV, Udut EV, Miroshnichenko LA, Simanina EV, Chaikovskii AV, Trofimova ES, Minakova MY, Udut VV, and Dygai AM

Subjects
Animals, Bone Marrow Cells cytology, Bone Marrow Cells drug effects, Bone Marrow Cells metabolism, Cell Differentiation drug effects, Cell Proliferation drug effects, Gene Expression Regulation, Mesenchymal Stem Cells cytology, Mesenchymal Stem Cells metabolism, Mice, Mice, Inbred CBA, Mitogen-Activated Protein Kinase 1 antagonists & inhibitors, Mitogen-Activated Protein Kinase 1 metabolism, Mitogen-Activated Protein Kinase 3 antagonists & inhibitors, Mitogen-Activated Protein Kinase 3 metabolism, Phosphatidylinositol 3-Kinases metabolism, Phosphoinositide-3 Kinase Inhibitors, Primary Cell Culture, Protein Kinase Inhibitors pharmacology, Signal Transduction, p38 Mitogen-Activated Protein Kinases metabolism, Fibroblast Growth Factor 2 pharmacology, Mesenchymal Stem Cells drug effects, Mitogen-Activated Protein Kinase 1 genetics, Mitogen-Activated Protein Kinase 3 genetics, Phosphatidylinositol 3-Kinases genetics, p38 Mitogen-Activated Protein Kinases genetics
Abstract

We studied PI3K, MAPK/EPK1/2 and p38-dependent signaling cascade under stimulation of the functions of mesenchymal progenitor cells by fibroblast growth factor. The important role of PI3K and EPK1/2 in the realization of their growth potential has been revealed. Experiments have demonstrated a blocking effect of PI3K inhibitor on proliferation and differentiation of progenitor cells and EPK1/2 inhibitor on mitotic activity of the progenitor cells. The p38-mediated MAPK pathway did not play a role in determining the proliferative and differentiation status of fibroblast CFU.

Published
2014
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3. Involvement of cAMP- and IKK-2-dependent signal pathways in the growth capacity of mesenchymal progenitor cells under the influence of basic fibroblast growth factor.

Author

Zyuz'kov GN, Zhdanov VV, Danilets MG, Udut EV, Miroshnichenko LA, Ligacheva AA, Simanina EV, Chaikovskii AV, Trofimova ES, Minakova MY, Udut VV, and Dygai AM

Subjects
Animals, Cells, Cultured, Mesenchymal Stem Cells cytology, Mice, Regenerative Medicine, Cyclic AMP metabolism, Fibroblast Growth Factor 2 pharmacology, I-kappa B Kinase metabolism, Mesenchymal Stem Cells drug effects, Mesenchymal Stem Cells metabolism, Signal Transduction physiology
Abstract

We studied the role of cAMP- and IKK-2-dependent pathways under conditions of functional stimulation of mesenchymal progenitor cells by basic fibroblast growth factor. An adenylate cyclase inhibitor 2',5'-dideoxyadenosine had no effect on functional activity of early (primitive) mesenchymal precursors. This agent produced a stimulatory effect on proliferation of stromal precursor cells. Mitotic activity of mesenchymal progenitor cells was shown to decrease after treatment with a specific blocker of the IKK-2 kinase-inhibitor complex.

Published
2014
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4. Participation of PI3K, MAPK ERK1/2, and p38 in the realization of growth potential of mesenchymal precursor cells under in vitro conditions.

Author

Zyuz'kov GN, Danilets MG, Ligacheva AA, Zhdanov VV, Udut EV, Miroshnichenko LA, Simanina EV, Trofimova ES, Minakova MY, Chaikovskii AV, Agafonov VI, and Dygai AM

Subjects
Animals, Cell Culture Techniques, Cell Proliferation, Cells, Cultured, Chromones pharmacology, Extracellular Signal-Regulated MAP Kinases antagonists & inhibitors, MAP Kinase Signaling System, Mesenchymal Stem Cells drug effects, Mice, Inbred C57BL, Morpholines pharmacology, Phosphoinositide-3 Kinase Inhibitors, Protein Kinase Inhibitors pharmacology, p38 Mitogen-Activated Protein Kinases antagonists & inhibitors, Extracellular Signal-Regulated MAP Kinases metabolism, Mesenchymal Stem Cells physiology, Phosphatidylinositol 3-Kinases metabolism, p38 Mitogen-Activated Protein Kinases metabolism
Abstract

We studied the role of intracellular signal molecules PI3K, MAPK ERK1/2, and p38 in the realization of the growth potential of mesenchymal progenitor elements. Under in vitro conditions, PI3K и ERK1/2 specifi c inhibitors reduced fi broblastic colony- and cluster-formation and considerably suppressed proliferative activity of mesenchymal precursors. Blocker of p38 and protein kinase B had no effect on the function of fi broblast CFU.

Published
2014
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5. Role of cAMP and IKK-2-dependent signaling in the realization of growth potential of mesenchymal progenitor cells.

Author

Zyuz'kov GN, Zhdanov VV, Danilets MG, Udut EV, Miroshnichenko LA, Ligacheva AA, Simanina EV, Trofimova ES, Minakova MY, Chaikovskii AV, and Dygai AM

Subjects
Adenylyl Cyclase Inhibitors, Animals, Bone Marrow Cells metabolism, Cell Differentiation drug effects, Cell Proliferation drug effects, Cells, Cultured, Colony-Forming Units Assay, Cyclic AMP metabolism, Fibroblasts drug effects, I-kappa B Kinase antagonists & inhibitors, Male, Mice, Mice, Inbred CBA, Signal Transduction drug effects, Adenylyl Cyclases metabolism, Dideoxyadenosine pharmacology, Hematopoietic Stem Cells metabolism, I-kappa B Kinase metabolism, Mesenchymal Stem Cells physiology
Abstract

We studied the role of cAMP- and IKK-2-mediated pathways in the realization of growth potential of mesenchymal progenitor cells in vitro. It had been found that adenylate cyclase inhibitor 2',5'-dideoxyadenosine had no effect on the proliferation and differentiation of fibroblastic CFU. A decrease in differentiation rate of progenitor cells was observed after the treatment with specific IKK-2 blocker inhibitor-kinase complex.

Published
2013
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6. Role of NF-κB-dependent signaling in the realization of growth potential of mesenchymal progenitor cells in vitro.

Author

Zyuz'kov GN, Danilets MG, Ligacheva AA, Zhdanov VV, Udut EV, Miroshnichenko LA, Simanina EV, Trofimova ES, Minakova MY, Chaikovskii AV, Agafonov VI, and Dygai AM

Subjects
Animals, Cell Differentiation, Cell Proliferation, Cells, Cultured, Male, Mice, Mice, Inbred CBA, Signal Transduction, Mesenchymal Stem Cells physiology, NF-kappa B metabolism
Abstract

Signal pathways of realization of growth potential of mesenchymal progenitor cells related to transcription factor NF-κB were studied in vitro. NF-κB was found to participate in the proliferation and differentiation of progenitor elements that can be blocked by its specific inhibitor oridonin. NF-κB inhibitor aurothiomalate had no effect on the functions of fibroblastic CFU.

Published
2013
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