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3 results on '"MESH: Vaccines, Subunit"'

1. Evaluation of protective effect of recombinant dense granule antigens GRA2 and GRA6 formulated in monophosphoryl lipid A (MPL) adjuvant against Toxoplasma chronic infection in mice

Author

Corinne Mercier, Sima Rafati, Karine Musset, Mehdi Assmar, Mohammad-Ali Shokrgozar, M. Reza Sadaie, Marie-France Cesbron-Delauw, Majid Golkar, Laboratoire Adaptation et pathogénie des micro-organismes [Grenoble] (LAPM), and Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF)

Subjects
Protozoan Vaccines, medicine.medical_treatment, Protozoan Proteins, Antibodies, Protozoan, Monophosphoryl Lipid A, MESH: Vaccines, Subunit, MESH: Adjuvants, Immunologic, MESH: Lipid A, law.invention, Mice, 0302 clinical medicine, law, MESH: Animals, MESH: Protozoan Proteins, Vaccines, Synthetic, 0303 health sciences, MESH: Cytokines, MESH: Mice, Inbred CBA, MESH: Toxoplasmosis, Animal, biology, MESH: Escherichia coli, MESH: Toxoplasma, Brain, 3. Good health, Vaccination, Lipid A, Infectious Diseases, medicine.anatomical_structure, Vaccines, Subunit, Recombinant DNA, Cytokines, Molecular Medicine, Antibody, Toxoplasma, Adjuvant, MESH: Vaccines, Synthetic, 030231 tropical medicine, Antigens, Protozoan, Spleen, Microbiology, 03 medical and health sciences, MESH: Brain, Adjuvants, Immunologic, Antigen, parasitic diseases, Escherichia coli, medicine, Animals, Humans, MESH: Antibodies, Protozoan, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, MESH: Mice, 030304 developmental biology, MESH: Humans, General Veterinary, General Immunology and Microbiology, MESH: Protozoan Vaccines, Public Health, Environmental and Occupational Health, Toxoplasma gondii, Th1 Cells, biology.organism_classification, Disease Models, Animal, Toxoplasmosis, Animal, MESH: Th1 Cells, Immunology, Mice, Inbred CBA, biology.protein, MESH: Disease Models, Animal, MESH: Antigens, Protozoan
Abstract

To investigate the vaccine potential of both the Toxoplasma GRA2 and GRA6 antigens, the full length recombinant proteins were produced in Escherichia coli, formulated in MPL adjuvant, and used alone and in combination ("mix"), to immunize CBA/J mice. Although high ratios of specific IgG2a/IgG1 were measured against both proteins, only spleen cells from GRA2-immunized mice and mix-immunized mice produced high amounts of both IFN-gamma and IL-2 upon induction with Toxoplasma gondii Excretory-Secretory Antigens. Intra peritoneal challenge with Toxoplasma cysts resulted in significant reduction of brain cysts in GRA2- and in mix-vaccinated mice only. This study shows the protective efficacy of recombinant GRA2 against chronic infection by T. gondii and confirms the utility of MPL adjuvant in enabling a vaccine candidate to induce a protective Th1 immune response.

Published
2007

2. Subunit recombinant vaccine protects against monkeypox

Author

Christopher Trindade, Jay W. Hooper, Aysegul Nalca, Peter Silvera, Genoveffa Franchini, Jean-Michel Heraud, Vaniambadi S. Kalyanaraman, Victor I. Ayala, Irene Kalisz, David Venzon, Barney S. Graham, Wen-Po Tsai, Anna Hryniewicz, Monica Vaccari, Mike Bray, Yvette Edghill-Smith, Meredith Hassett, Jody Manischewitz, Hana Golding, Lisa R. King, Janie Parrino, National Cancer Institute [Bethesda] (NCI-NIH), National Institutes of Health [Bethesda] (NIH), Advanced Bioscience Laboratories (ABL), National Institute of Allergy and Infectious Diseases [Bethesda] (NIAID-NIH), U.S. Food and Drug Administration (FDA), University of Bialystok, U.S. Army Medical Research Institute of Infectious Diseases, Southern Research Institute [Birmingham, USA], This work was supported by the Intramural Research Program of the National Institutes of Health, National Cancer Institute, Center for Cancer Research, the National Institute of Allergy and Infectious Diseases Intramural Biodefense Research Award program, and and National Institutes of Health Contract N01-AI-15451.

Subjects
MESH: Monkeypox, viruses, MESH: Vaccines, Subunit, MESH: Amino Acid Sequence, Antibodies, Viral, law.invention, Dryvax, chemistry.chemical_compound, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, law, Immunology and Allergy, MESH: Animals, Monkeypox virus, Smallpox vaccine, Antigens, Viral, [SDV.MHEP.ME]Life Sciences [q-bio]/Human health and pathology/Emerging diseases, 0303 health sciences, Vaccines, Synthetic, virus diseases, 3. Good health, Titer, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, Vaccines, Subunit, Recombinant DNA, [SDV.IMM]Life Sciences [q-bio]/Immunology, MESH: Antigens, Viral, Smallpox Vaccine, MESH: Vaccines, Synthetic, Immunology, Molecular Sequence Data, Biology, Virus, MESH: Macaca mulatta, 03 medical and health sciences, Monkeypox, MESH: Viral Vaccines, medicine, Smallpox, Animals, Amino Acid Sequence, 030304 developmental biology, MESH: Molecular Sequence Data, 030306 microbiology, [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, Viral Vaccines, medicine.disease, MESH: Smallpox Vaccine, Virology, Macaca mulatta, MESH: DNA, Viral, Disease Models, Animal, MESH: Monkeypox virus, chemistry, DNA, Viral, MESH: Disease Models, Animal, [SDV.IMM.VAC]Life Sciences [q-bio]/Immunology/Vaccinology, Vaccinia, MESH: Antibodies, Viral
Abstract

The smallpox vaccine Dryvax, a live vaccinia virus (VACV), protects against smallpox and monkeypox, but is contraindicated in immunocompromised individuals. Because Abs to VACV mediate protection, a live virus vaccine could be substituted by a safe subunit protein-based vaccine able to induce a protective Ab response. We immunized rhesus macaques with plasmid DNA encoding the monkeypox orthologs of the VACV L1R, A27L, A33R, and B5R proteins by the intradermal and i.m. routes, either alone or in combination with the equivalent recombinant proteins produced in Escherichia coli. Animals that received only DNA failed to produce high titer Abs, developed innumerable skin lesions after challenge, and died in a manner similar to placebo controls. By contrast, the animals vaccinated with proteins developed moderate to severe disease (20–155 skin lesions) but survived. Importantly, those immunized with DNA and boosted with proteins had mild disease with 15 or fewer lesions that resolved within days. DNA/protein immunization elicited Th responses and binding Ab titers to all four proteins that correlated negatively with the total lesion number. The sera of the immunized macaques recognized a limited number of linear B cell epitopes that are highly conserved among orthopoxviruses. Their identification may guide future efforts to develop simpler, safer, and more effective vaccines for monkeypox and smallpox.

Published
2006

3. Chemoimmunotherapy of tumors: cyclophosphamide synergizes with exosome based vaccines

Author

Julien, Taieb, Nathalie, Chaput, Noël, Schartz, Stéphan, Roux, Sophie, Novault, Cédric, Ménard, François, Ghiringhelli, Magali, Terme, Antoine F, Carpentier, Guillaume, Darrasse-Jèze, Guillaume, Darrasse-Jèse, François, Lemonnier, Laurence, Zitvogel, Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Gustave Roussy (IGR), Service d'Hépato-Gastro-Entérologie [CHU Pitié-Salpêtrière], CHU Pitié-Salpêtrière [AP-HP], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Mort cellulaire et cancer, Université de Bourgogne (UB)-IFR100 - Structure fédérative de recherche Santé-STIC-Institut National de la Santé et de la Recherche Médicale (INSERM), Service de Neurologie [CHU Pitié-Salpêtrière], IFR70-CHU Pitié-Salpêtrière [AP-HP], Biologie et thérapeutique des pathologies immunitaires (BTPI), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS), Immunité Cellulaire Antivirale, Institut Pasteur [Paris], This work is supported by a Poste d’accueil Institut National de la Santé et de la Recherche Médicale and Assistance Publique Hopitaux de Paris (to J.T.) and is supported by a European fellowship in the QLRT-2001-00093 and by Association de Recherche contre le Cancer (to N.C.). This work has also been supported by the EC ALLOSTEM grant, by DC THERA European grant, by the Association de Recherche contre le Cancer, and by the LIGUE LABELLISEE française contre le Cancer cancéropôle, Ile de France., Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Institut Pasteur [Paris] (IP), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), and Novault, Sophie

Subjects
medicine.medical_treatment, [SDV]Life Sciences [q-bio], Melanoma, Experimental, MESH: Vaccines, Subunit, MESH: Adjuvants, Immunologic, CD8-Positive T-Lymphocytes, Pharmacology, MESH: Antineoplastic Agents, Alkylating, Immunotherapy, Adoptive, T-Lymphocytes, Regulatory, MESH: Mice, Knockout, MESH: Cancer Vaccines, Mice, 0302 clinical medicine, MESH: Cytoplasmic Vesicles, polycyclic compounds, Immunology and Allergy, MESH: Animals, IL-2 receptor, Cells, Cultured, Mice, Knockout, Mice, Inbred BALB C, MESH: Dendritic Cells, FOXP3, MESH: CD8-Positive T-Lymphocytes, 3. Good health, Killer Cells, Natural, [SDV] Life Sciences [q-bio], MESH: Melanoma, Experimental, medicine.anatomical_structure, 030220 oncology & carcinogenesis, Vaccines, Subunit, MESH: Immunotherapy, Adoptive, Female, hormones, hormone substitutes, and hormone antagonists, MESH: Cells, Cultured, MESH: Killer Cells, Natural, MESH: Mice, Transgenic, T cell, Immunology, MESH: Mice, Inbred BALB C, Mice, Transgenic, Biology, Major histocompatibility complex, Cancer Vaccines, Exosome, 03 medical and health sciences, Adjuvants, Immunologic, medicine, Animals, Humans, Antineoplastic Agents, Alkylating, Cyclophosphamide, MESH: Mice, MESH: Humans, MESH: T-Lymphocytes, Regulatory, Cytoplasmic Vesicles, MESH: Cyclophosphamide, Dendritic Cells, Immunotherapy, Microvesicles, CTL, biology.protein, MESH: Female, 030215 immunology
Abstract

Dendritic cell-derived exosomes (DEX) are nanomeric vesicles harboring MHC/peptide complexes capable of promoting primary T cell responses and tumor rejection in the presence of adjuvants. In this study, we show that, in the absence of adjuvants, DEX mediate potent Ag-dependent antitumor effects against preestablished tumors in mice pretreated with immunopotentiating dosing of cyclophosphamide. Cyclophosphamide could 1) abolish the suppressive function of CD4+CD25+Foxp3+ regulatory T cells, 2) markedly enhance the magnitude of secondary but not primary CTL responses induced by DEX vaccines, 3) synergize with DEX in therapy but not prophylaxis tumor models. Therefore, therapeutic vaccines such as DEX aimed at boosting tumor-primed effector T cells could benefit procedures that minimize the effects of CD4+CD25+ regulatory T cells.

Published
2006

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