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23 results on '"MESH: Equipment Failure Analysis"'

1. X-ray spectroscopy and X-ray diffraction at wavelengths near theK-absorption edge of phosphorus

Author

Corinne Mas, Gérard Brandolin, J. Vicat, Richard Kahn, Heinrich Stuhrmann, Hugues Nury, Valérie Biou, Eva Pebay-Peyroula, Jean Marie Bois, Lionel Nauton, Peter Bösecke, Laboratoire d'enzymologie et biochimie structurales (LEBS), Centre National de la Recherche Scientifique (CNRS), Laboratoire de Mathématiques de Reims (LMR), Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS), Biochimie et biophysique des systèmes intégrés (BBSI), Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Joseph Fourier - Grenoble 1 (UJF), Institut de biologie structurale (IBS - UMR 5075 ), Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS), Université Joseph Fourier - Grenoble 1 (UJF)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), and Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)

Subjects
Halobacterium, Diffraction, Nuclear and High Energy Physics, Absorption spectroscopy, Protein Conformation, Carbon-Oxygen Lyases, Neutron diffraction, Molecular Conformation, Analytical chemistry, MESH: Equipment Failure Analysis, 01 natural sciences, MESH: Carbon-Oxygen Lyases, Crystal, 03 medical and health sciences, MESH: Protein Conformation, Purple Membrane, X-Ray Diffraction, 0103 physical sciences, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], MESH: Phosphorus, Instrumentation, 030304 developmental biology, 010302 applied physics, MESH: Molecular Conformation, 0303 health sciences, X-ray spectroscopy, Radiation, Chemistry, MESH: X-Ray Diffraction, Spectrometry, X-Ray Emission, MESH: Spectrometry, X-Ray Emission, Phosphorus, Equipment Design, MESH: Purple Membrane, Equipment Failure Analysis, Wavelength, Crystallography, Absorption edge, X-ray crystallography, MESH: Halobacterium, MESH: Mitochondrial ADP, ATP Translocases, Mitochondrial ADP, ATP Translocases, MESH: Equipment Design
Abstract

International audience; Phosphorus is an abundant element in living organisms. It is traceable by its X-ray absorption spectrum which shows a strong white line at its K-edge, comparable with that observed for the L(III) edges of rare earth ions. With purple membrane, the variation of the imaginary part of the anomalous dispersion of phosphorus is found to be close to 20 anomalous electron units. Anomalous diffraction experiments at wavelengths near the K-absorption edge of phosphorus confirm this result. The spatial distribution of lipids derived from anomalous diffraction agrees with earlier results from neutron diffraction. Test experiments on single crystals of the carrier protein using 5.76 A photons gave a first low-resolution diffraction pattern. Various techniques of crystal mounting were attempted. In addition, fluorescence measurements on a solution of threonine synthase appear to hint at a change of the phosphate environment of the cofactor upon activator binding.

Published
2005
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2. Computed tomography image processing to detect the real mechanism of bioprosthesis failure: implication for valve-in-valve implantation

Author

Ruggieri, V. G., Amedeo Anselmi, Wang, Q., Esneault, S., Haigron, P., Verhoye, J. -P, Service de chirurgie thoracique cardiaque et vasculaire [Rennes] = Thoracic and Cardiovascular Surgery [Rennes], CHU Pontchaillou [Rennes], Service de chirurgie thoracique cardiaque et vasculaire [Rennes], Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Hôpital Pontchaillou-CHU Pontchaillou [Rennes], and Le Corre, Morgane

Subjects
Male, Reoperation, MESH: Heart Valve Prosthesis Implantation, MESH: Heart Valve Prosthesis, [INFO.INFO-TS] Computer Science [cs]/Signal and Image Processing, MESH: Equipment Failure Analysis, MESH: Calcinosis, MESH: Reoperation, [INFO.INFO-TS]Computer Science [cs]/Signal and Image Processing, Humans, MESH: Aortic Valve Stenosis, [SPI.SIGNAL] Engineering Sciences [physics]/Signal and Image processing, [SDV.IB] Life Sciences [q-bio]/Bioengineering, Heart Valve Prosthesis Implantation, MESH: Humans, MESH: Middle Aged, Calcinosis, Aortic Valve Stenosis, Middle Aged, MESH: Male, Prosthesis Failure, Equipment Failure Analysis, Aortic Valve, Heart Valve Prosthesis, MESH: Prosthesis Failure, [SDV.IB]Life Sciences [q-bio]/Bioengineering, Tomography, X-Ray Computed, MESH: Aortic Valve, MESH: Tomography, X-Ray Computed, [SPI.SIGNAL]Engineering Sciences [physics]/Signal and Image processing
Abstract

International audience; BACKGROUND AND AIM OF THE STUDY: Transcatheter valve-in-valve implantation is an emerging option for patients with structural deterioration of an aortic bioprosthesis and who are at a high surgical risk. The present case underlines the need for dedicated imaging to accurately understand the mechanism of valve failure, and the feasibility of a valve-in-valve procedure. METHODS: A patient with structural bioprosthetic deterioration at echocardiography was investigated using computed tomography (CT) scanning and novel 3D slicer software. The findings were compared with those revealed at intraoperative pathology. RESULTS: Post-processed CT images showed the bulk of calcifications to be located at the subvalvular level, suggesting the presence of calcified pannus. Pathology of the explanted valve confirmed that the valve stenosis was due primarily to pannus. Misdiagnosed calcified pannus represents a major threat during valve-in-valve procedures, due mainly to embolic risk. CONCLUSION: The 3D slicer elaboration of CT images may be invaluable in providing a precise definition of the mechanism of valve failure, and also to establish the feasibility of either a valve-in-valve procedure or conventional surgery.

Published
2013

3. Peptide binding to ochratoxin A mycotoxin: a new approach in conception of biosensors

Author

M. De Waard, Jean-Marc Sabatier, A. Ibn Hadj Hassine, Nicolas Andreotti, Catherine Gonzalez, Ingrid Bazin, IMT - MINES ALES (IMT - MINES ALES), Institut Mines-Télécom [Paris] (IMT), Gènes HLA-DR, Autoanticorps et Microchimérisme dans la Polyarthrite Rhumatoïde et la Sclérodermie (HLA-DR), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), INSERM U836, équipe 3, Canaux calciques, fonctions et pathologies, Grenoble Institut des Neurosciences (GIN), Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM), Labex ICST, Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Aix Marseille Université (AMU), and Canepari, Marco

Subjects
Ochratoxin A, MESH: Equipment Failure Analysis, Peptide, Peptide binding, Biosensing Techniques, 02 engineering and technology, 01 natural sciences, Chemical synthesis, mycotoxin, chemistry.chemical_compound, Protein Interaction Mapping, Electrochemistry, chemistry.chemical_classification, Mimotope, MESH: Peptides, MESH: Enzyme-Linked Immunosorbent Assay, MESH: Ochratoxins, Equipment Design, General Medicine, 021001 nanoscience & nanotechnology, Ochratoxins, MESH: Reproducibility of Results, Biochemistry, [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], 0210 nano-technology, ochratoxin A, MESH: Biosensing Techniques, Biotechnology, Biomedical Engineering, Biophysics, Enzyme-Linked Immunosorbent Assay, MESH: Mycotoxins, Sensitivity and Specificity, [SDV.BBM] Life Sciences [q-bio]/Biochemistry, Molecular Biology, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, [SDV.NEU] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], Mycotoxin, peptide-based assay, Ochratoxin, Chromatography, 010401 analytical chemistry, MESH: Protein Interaction Mapping, Reproducibility of Results, Mycotoxins, MESH: Sensitivity and Specificity, 0104 chemical sciences, Equipment Failure Analysis, Enzyme, chemistry, Peptides, peptide binding, MESH: Equipment Design
Abstract

International audience; Ochratoxin A (OTA) is a widespread and abundant natural carcinogenic mycotoxin produced by several species of Aspergillus and Penicillium fungi. Due to the ubiquitous presence of these fungi in food and potential risk for human health, a rapid and sensitive in vitro detection assay is required. Analytical methods for OTA detection/identification are generally based on liquid-liquid extraction, clean-up using an immunoaffinity column (IAC), and identification by reversed-phase high pressure liquid chromatography with fluorescence detection (HPLC-FLD). However, IACs are costly and have a short lifespan. Therefore, an interesting approach would appear to be the design and chemical synthesis of a mimotope peptide simulating mycotoxin-specific antibodies. We have developed a promising alternative method that is based on the use of peptides which are able to bind to specific chemical functions and/or molecular structures. Accordingly, a number of peptides (derived from the structures of major redox proteins) were selected and produced by chemical solid phase syntheses. The ability of such peptides to bind to ochratoxin A was evaluated by HPLC. The peptide NF04 (structurally derived from an oxidoreductase enzyme), which was found to be the sole potently reactive compound among tested molecules, was further evaluated in a peptide-based enzyme-linked immunosorbent assay (peptide-based ELISA), thus confirming its specific interaction with ochratoxin A.

Published
2013
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4. On chip real time monitoring of B-cells hybridoma secretion of immunoglobulin

Author

Patrice N. Marche, Sarah Milgram, Sandra Cortès, Marie-Bernadette Villiers, Yoann Roupioz, Arnaud Buhot, Thierry Livache, Structures et propriétés d'architectures moléculaire (SPRAM - UMR 5819), Institut Nanosciences et Cryogénie (INAC), Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), INSERM U823, équipe 8 (Immunologie Analytique des Pathologies Chroniques), Institut d'oncologie/développement Albert Bonniot de Grenoble (INSERM U823), Institut National de la Santé et de la Recherche Médicale (INSERM)-EFS-CHU Grenoble-Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM)-EFS-CHU Grenoble-Université Joseph Fourier - Grenoble 1 (UJF), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Université Joseph Fourier - Grenoble 1 (UJF)-CHU Grenoble-EFS-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Joseph Fourier - Grenoble 1 (UJF)-CHU Grenoble-EFS-Institut National de la Santé et de la Recherche Médicale (INSERM), and Marche, Patrice

Subjects
MESH: Immunoglobulins, Cell Culture Techniques, MESH: Equipment Failure Analysis, Biosensing Techniques, 01 natural sciences, Real-time detection, Electrochemistry, Biochip, Immunoassay, 0303 health sciences, B-Lymphocytes, MESH: Protein Array Analysis, General Medicine, Equipment Design, Protein micro-array, MESH: Surface Plasmon Resonance, medicine.anatomical_structure, Biochemistry, [SDV.IMM]Life Sciences [q-bio]/Immunology, Antibody, MESH: Immunoassay, MESH: Biosensing Techniques, Biotechnology, [SDV.IMM] Life Sciences [q-bio]/Immunology, MESH: Hybridomas, Biomedical Engineering, Biophysics, Protein Array Analysis, Immunoglobulins, Biology, Cell Line, Surface plasmon resonance imaging, 03 medical and health sciences, Immune system, Antigen, MESH: B-Lymphocytes, medicine, Humans, Secretion, B cell, 030304 developmental biology, MESH: Cell Culture Techniques, Hybridomas, MESH: Humans, Blood cell, 010401 analytical chemistry, Cellular secretion, Surface Plasmon Resonance, Molecular biology, In vitro, 0104 chemical sciences, MESH: Cell Line, Equipment Failure Analysis, biology.protein, Biosensor, MESH: Equipment Design
Abstract

International audience; The secretions of molecules by cells are of tremendous interest for both fundamental insights studies and medical purposes. In this study, we propose a new biochip-based approach for the instantaneous monitoring of protein secretions, using antibody production by B lymphocytes cultured in vitro. This was possible thanks to the Surface Plasmon Resonance imaging (SPRi) of a protein biochip where antigen proteins (Hen Egg Lysozyme, HEL) were micro-arrayed along with series of control proteins. B cell hybridomas were cultured on the chip and the secretion of immunoglobulins (antibody) specific to HEL was monitored in real-time and detected within only few minutes rather than after a 30-60 min incubation with standard ELISA experiments. This fast and sensitive detection was possible thanks to the sedimentation of the cells on the biochip sensitive surface, where local antibody concentrations are much higher before dilution in the bulk medium. An other interesting feature of this approach for the secretion monitoring was the independence of the SPR response--after normalization--regarding to the density of the surface-immobilized probes. Such biosensor might thus pave the way to new tools capable of both qualitative and semi-quantitative analysis of proteins secreted by other immune cells.

Published
2011
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5. Effect of manufacturer-inserted mask leaks on ventilator performance

Author

Karl Leroux, Bruno Louis, Brigitte Fauroux, Frédéric Lofaso, Daniel Isabey, INSERM U955, équipe 13, Institut Mondor de Recherche Biomédicale (IMRB), Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), ADEP assistance, CHU Trousseau [APHP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Centre de Recherche Saint-Antoine (UMRS893), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Centre d’Investigation Clinique 1429 [Garches] (CIC 1429), Hôpital Raymond Poincaré [AP-HP]-Institut National de la Santé et de la Recherche Médicale (INSERM)-Hôpital Raymond Poincaré [AP-HP]-Institut National de la Santé et de la Recherche Médicale (INSERM), Association d'Entraide des Polios et Handicapés (ADEP), Service de pneumologie [CHU Trousseau], Université Pierre et Marie Curie - Paris 6 (UPMC)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-CHU Trousseau [APHP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Sorbonne Université (SU), and Guellaen, Georges

Subjects
Pulmonary and Respiratory Medicine, Adult, Leak, medicine.medical_specialty, Patient-ventilator synchrony, Pulmonary disease, MESH: Equipment Failure Analysis, Mascara, MESH: Ventilators, Mechanical, law.invention, Positive-Pressure Respiration, 03 medical and health sciences, MESH: Positive-Pressure Respiration, 0302 clinical medicine, law, [SDV.BBM] Life Sciences [q-bio]/Biochemistry, Molecular Biology, Medicine, Humans, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, 030212 general & internal medicine, Simulation, Ventilators, Mechanical, MESH: Humans, MESH: Middle Aged, business.industry, Masks, MESH: Adult, Equipment Design, Middle Aged, Leaks, Interface, Surgery, Equipment Failure Analysis, On ventilator, Trigger, 030228 respiratory system, MESH: Masks, Ventilation (architecture), Noninvasive ventilation, business, MESH: Equipment Design
Abstract

International audience; Most pressure-support devices use a single circuit with an exhalation port integrated in the mask. The aim of the current study was to compare the effects of masks having different manufacturer-inserted leaks on ventilator performance. We simulated chronic obstructive pulmonary disease and restrictive disease. Four ventilators (VENTImotion (Weinmann, Hamburg, Germany), VPAP III STA (ResMed, Saint Priest, France), Synchrony 2 (Respironics, Nantes, France) and Vivo 40 (Breas, Saint Priest)) were tested with the recommended masks and with the masks having the largest and smallest leaks. Tests were performed with pressure support levels of 10, 15 and 20 cmH(2)O. The in vivo evaluation compared two ventilators using recommended masks opposed in terms of exhaled port resistance. The ventilators were tested with their recommended mask, and after mask exchange. The mask with the largest leak induced auto-triggering and/or increased inspiratory-trigger sensitivity was the VENTImotion under both simulated conditions and VPAP III STA under the simulated obstructive-disease condition. The mask with the smallest leak-increased inspiratory-trigger delay was Synchrony 2 in the simulated obstructive-disease condition and increased rebreathing. The in vivo study confirmed the bench results. When switching to a mask that has a different leak, evaluation is needed to adjust trigger sensitivity and pressurisation level and to check the absence of rebreathing.

Published
2010
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6. Pressure stability with CPAP devices: A bench evaluation

Author

Frédéric Lofaso, Véronique Grillier-Lanoir, Karl Leroux, Bruno Louis, Brigitte Fauroux, Maud Boucherie, Daniel Isabey, Institut Mondor de recherche biomédicale (IMRB), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), ADEP assistance, Respiratory division, Covidien, Service de pneumologie [CHU Trousseau], Université Pierre et Marie Curie - Paris 6 (UPMC)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-CHU Trousseau [APHP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Sorbonne Université (SU), Centre de Recherche Saint-Antoine (UMRS893), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre d’Investigation Clinique 1429 [Garches] (CIC 1429), Hôpital Raymond Poincaré [AP-HP]-Institut National de la Santé et de la Recherche Médicale (INSERM), Guellaen, Georges, CHU Trousseau [APHP], and Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)

Subjects
Leak, MESH: Continuous Positive Airway Pressure, obstructive sleep apnoea, medicine.medical_treatment, MESH: Equipment Failure Analysis, Pulmonary compliance, MESH: Pulmonary Ventilation, leak effects, Positive-Pressure Respiration, 03 medical and health sciences, Work of breathing, MESH: Positive-Pressure Respiration, 0302 clinical medicine, Airway resistance, MESH: Computer Simulation, MESH: Airway Resistance, work of breathing, MESH: Humidity, [SDV.BBM] Life Sciences [q-bio]/Biochemistry, Molecular Biology, medicine, Humans, Computer Simulation, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, Continuous positive airway pressure, Lung Compliance, bench test, Pressure drop, MESH: Humans, business.industry, Airway Resistance, Humidity, General Medicine, respiratory tract diseases, Equipment Failure Analysis, Compliance (physiology), MESH: Work of Breathing, 030228 respiratory system, Constant pressure, Anesthesia, Pulmonary Ventilation, business, MESH: Lung Compliance, 030217 neurology & neurosurgery, continuous positive airway pressure
Abstract

International audience; BACKGROUND: Continuous positive airway pressure (CPAP) maintains a constant pressure to reduce the patient's work of breathing (WOB). The aim of this study was to measure the additional WOB imposed by four current CPAP devices during simulation of a difficult but commonly encountered clinical situation. METHOD: Flow contour, respiratory system compliance and total lung-airway resistance of a patient under CPAP were simulated. The devices were tested at a CPAP of 15cm H(2)O with a heated humidifier and a nasal pillow, which increased circuitry resistance and with and without a simulated unintentional leak. RESULTS: With no leak, positive end-expiratory pressure (PEEP) at the interface varied across devices from 14.0 to 15.3cm H(2)O. With a leak of 1L/s, PEEP varied from 11.5 to 17.1cm H(2)O. Imposed inspiratory WOB ranged from less than 0.1J/min to 0.45J/min with no leak, and the range broadened with leaking. Findings were similar for the imposed expiratory WOB. CONCLUSION: The performances of CPAP devices are variable. The device that calibrated for the pressure loss in the circuitry under dynamic conditions and made appropriate pressure adjustments outperformed the other devices.

Published
2010
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7. A minimally invasive microdevice for molecular sampling and analysis

Author

P. Caillat, François Berger, Ali Bouamrani, F. Martin, M. Cosnier, Commissariat à l'énergie atomique et aux énergies alternatives - Laboratoire d'Electronique et de Technologie de l'Information (CEA-LETI), Direction de Recherche Technologique (CEA) (DRT (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Grenoble Institut des Neurosciences (GIN), Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM), 'Canceropole Lyon Auvergne Rhone Alpes' Nanobio innovation pole, and Issartel, Jean-Paul

Subjects
Computer science, Biopsy, Biomedical Engineering, Oncological surgery, MESH: Equipment Failure Analysis, Nanotechnology, 01 natural sciences, Sensitivity and Specificity, 03 medical and health sciences, MESH: Biopsy, Biopolymers, Biopsy device, Tissue damage, medicine, Microtechnology, Minimally Invasive Surgical Procedures, 030304 developmental biology, [SDV.IB] Life Sciences [q-bio]/Bioengineering, 0303 health sciences, Miniaturization, medicine.diagnostic_test, 010401 analytical chemistry, Cancer, Reproducibility of Results, MESH: Computer-Aided Design, Equipment Design, MESH: Miniaturization, medicine.disease, Microarray Analysis, MESH: Sensitivity and Specificity, 3. Good health, 0104 chemical sciences, Molecular analysis, Equipment Failure Analysis, MESH: Reproducibility of Results, MESH: Biopolymers, MESH: Microarray Analysis, Tissue Array Analysis, Chemical functionalization, Computer-Aided Design, MESH: Surgical Procedures, Minimally Invasive, [SDV.IB]Life Sciences [q-bio]/Bioengineering, MESH: Tissue Array Analysis, Biomedical engineering, MESH: Equipment Design
Abstract

International audience; In this paper, we present a new minimally invasive biopsy microdevice adapted to proteomic mass spectrometry analysis. The concept is born from a multidisciplinary collaboration in fields of proteomics, cancer research, and microtechnology. In mixing different skills, we have developed and manufactured a miniaturized biopsy device using microtechnology techniques in order to minimize tissue damage during surgical gesture. Dedicated chemically functionalized areas were added to the device in order to increase capture yield and specificity during tissue contact. Fields of application range from cancer research to the study of neurodegenerative diseases.

Published
2009
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8. A dedicated two-channel phased-array receiver coil for high-resolution MRI of the rat knee cartilage at 7 T

Author

A. Rengle, Hervé Saint-Jalmes, Olivier Beuf, J.-C. Goebel, M. Armenean, Pierre Gillet, Astrid Pinzano-Watrin, Radu Bolbos, Centre de Recherche et d'Application en Traitement de l'Image et du Signal (CREATIS), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-École Supérieure Chimie Physique Électronique de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Physiopathologie, Pharmacologie et Ingénierie articulaires (PPIA), Université Henri Poincaré - Nancy 1 (UHP)-Centre National de la Recherche Scientifique (CNRS), Laboratoire Traitement du Signal et de l'Image (LTSI), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université de Lyon-Université de Lyon-École Supérieure de Chimie Physique Électronique de Lyon (CPE)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), and Université de Rennes (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM)

Subjects
Cartilage, Articular, Phased array, [SDV.IB.IMA]Life Sciences [q-bio]/Bioengineering/Imaging, MESH: Equipment Failure Analysis, 02 engineering and technology, Knee Joint, 030218 nuclear medicine & medical imaging, MESH: Magnetic Resonance Imaging, 0302 clinical medicine, Data acquisition, rat cartilage morphology, [INFO.INFO-TS]Computer Science [cs]/Signal and Image Processing, MESH: Animals, Image resolution, medicine.diagnostic_test, MESH: Hindlimb, Equipment Design, Magnetic Resonance Imaging, Knee cartilage, Hindlimb, MESH: Reproducibility of Results, high-resolution MRI (HR-MRI), [SPI.ELEC]Engineering Sciences [physics]/Electromagnetism, [INFO.INFO-TI]Computer Science [cs]/Image Processing [eess.IV], [PHYS.PHYS.PHYS-MED-PH]Physics [physics]/Physics [physics]/Medical Physics [physics.med-ph], Computer-Aided Design, [SDV.IB]Life Sciences [q-bio]/Bioengineering, MESH: Image Enhancement, [SPI.SIGNAL]Engineering Sciences [physics]/Signal and Image processing, MESH: Transducers, Materials science, Channel (digital image), MESH: Rats, 0206 medical engineering, Transducers, Biomedical Engineering, Sensitivity and Specificity, multiple acquisitions, 03 medical and health sciences, Magnetics, medicine, [INFO.INFO-IM]Computer Science [cs]/Medical Imaging, Animals, MESH: Magnetics, phased-array coil, [SPI.ACOU]Engineering Sciences [physics]/Acoustics [physics.class-ph], Reproducibility of Results, MESH: Computer-Aided Design, Magnetic resonance imaging, Image Enhancement, 020601 biomedical engineering, MESH: Sensitivity and Specificity, Rats, Equipment Failure Analysis, High magnetic field strength, Signal-to-noise ratio (imaging), MESH: Cartilage, Articular, [SPI.OPTI]Engineering Sciences [physics]/Optics / Photonic, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology, Biomedical engineering, MESH: Equipment Design
Abstract

International audience; In the field of small animal studies, the array coil imaging has become increasingly important. In this paper, a dedicated two-channel array coil operating at 300 MHz (7 T) for high-resolution MRI (HR-MRI) of the rat knee cartilage is presented. The average gain in signal-to-noise ratio (SNR) compared to a 15-mm multipurpose surface coil was 2.2. This SNR gain was used to improve the spatial resolution of 3-D acquisitions by decreasing the voxel size from 59 x 59 x 156 microm(3) to 51 x 51 x 94 microm(3) without time penalty. Also, a set of two array coils was used to perform a simultaneous acquisition of both knee joints of a rat, maintaining the same scanning time without SNR or spatial resolution degradation compared to the single knee joint acquisition. This two-channel array coil is a key element to perform HR-MRI and extract cartilage morphological parameters such as thickness and volume.

Published
2009
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9. Energy-based adaptive focusing of waves: application to noninvasive aberration correction of ultrasonic wavefields

Author

Gabriel Montaldo, Mathieu Pernot, Eric Herbert, Mathias Fink, Mickael Tanter, Physique des ondes pour la médecine, Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut Langevin - Ondes et Images (UMR7587) (IL), Sorbonne Université (SU)-Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Paris (UP)-Centre National de la Recherche Scientifique (CNRS), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), and Pernot, Mathieu

Subjects
Acoustics and Ultrasonics, Acoustics, MESH: Equipment Failure Analysis, 01 natural sciences, Sensitivity and Specificity, Article, 030507 speech-language pathology & audiology, 03 medical and health sciences, Speckle pattern, Nonlinear acoustics, Optics, 0103 physical sciences, Electrical and Electronic Engineering, 010301 acoustics, Instrumentation, Ultrasonography, Physics, [SDV.IB] Life Sciences [q-bio]/Bioengineering, Hydrophone, business.industry, Phase distortion, Reproducibility of Results, MESH: Computer-Aided Design, Acoustic wave, Equipment Design, Image Enhancement, Sound intensity, MESH: Sensitivity and Specificity, Equipment Failure Analysis, MESH: Reproducibility of Results, MESH: Artifacts, Computer-Aided Design, Ultrasonic sensor, [SDV.IB]Life Sciences [q-bio]/Bioengineering, Laser beam quality, MESH: Image Enhancement, 0305 other medical science, business, Artifacts, MESH: Equipment Design, MESH: Ultrasonography
Abstract

International audience; An aberration correction method based on the maximization of the wave intensity at the focus of an emitting array is presented. The potential of this new adaptive focusing technique is investigated for ultrasonic focusing in biological tissues. The acoustic intensity is maximized noninvasively through direct measurement or indirect estimation of the beam energy at the focus for a series of spatially coded emissions. For ultrasonic waves, the acoustic energy at the desired focus can be indirectly estimated from the local displacements induced in tissues by the ultrasonic radiation force of the beam. Based on the measurement of these displacements, this method allows determination of the precise estimation of the phase and amplitude aberrations, and consequently the correction of aberrations along the beam travel path. The proof of concept is first performed experimentally using a large therapeutic array with strong electronic phase aberrations (up to 2pi). Displacements induced by the ultrasonic radiation force at the desired focus are indirectly estimated using the time shift of backscattered echoes recorded on the array. The phase estimation is deduced accurately using a direct inversion algorithm which reduces the standard deviation of the phase distribution from sigma = 1.89 radian before correction to sigma = 0.53 radian following correction. The corrected beam focusing quality is verified using a needle hydrophone. The peak intensity obtained through the aberrator is found to be -7.69 dB below the reference intensity obtained without any aberration. Using the phase correction, a sharp focus is restored through the aberrator with a relative peak intensity of -0.89 dB. The technique is tested experimentally using a linear transmit/receive array through a real aberrating layer. The array is used to automatically correct its beam quality, as it both generates the radiation force with coded excitations and indirectly estimates the acoustic intensity at the focus with speckle tracking. This technique could have important implications in the field of high-intensity focused ultrasound even in complex configurations such as transcranial, transcostal, or deep seated organs.

Published
2009
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10. Clinical and biological assessment of cemented titanium femoral stems: an 11-year experience

Author

Marc-Antoine Rousseau, Yves Catonné, J. Poupon, Jean-Yves Lazennec, Patrick Boyer, Philippe Ravaud, Service de Chirurgie Orthopédique et traumatique, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-AP-HP - Hôpital Bichat - Claude Bernard [Paris], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), CHU Pitié-Salpêtrière [AP-HP], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Hôpital Lariboisière, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Lariboisière-Fernand-Widal [APHP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Université Paris Diderot - Paris 7 (UPD7), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), and Gillet, Catherine

Subjects
Male, Osteolysis, MESH: Cementation, Arthroplasty, Replacement, Hip, MESH: Equipment Failure Analysis, Postoperative Complications, 0302 clinical medicine, MESH: Postoperative Complications, Medicine, MESH: Arthroplasty, Replacement, Hip, Orthopedics and Sports Medicine, Prospective Studies, 030212 general & internal medicine, Cementation, 030222 orthopedics, MESH: Middle Aged, Bone Cements, Middle Aged, Prosthesis Failure, Titanium oxide, MESH: Titanium, Female, MESH: Prosthesis Design, Titanium, Adult, Reoperation, musculoskeletal diseases, medicine.medical_specialty, Aseptic loosening, chemistry.chemical_element, MESH: Bone Cements, Prosthesis Design, MESH: Reoperation, 03 medical and health sciences, Cement mantle, Humans, In patient, titanium, femoral stem, Survival rate, Original Paper, corrosion, MESH: Humans, total hip prosthesis, business.industry, technology, industry, and agriculture, MESH: Adult, medicine.disease, equipment and supplies, MESH: Prospective Studies, MESH: Male, Surgery, Equipment Failure Analysis, chemistry, [SDV.SPEE] Life Sciences [q-bio]/Santé publique et épidémiologie, Orthopedic surgery, MESH: Prosthesis Failure, MESH: Hip Prosthesis, [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie, Hip Prosthesis, business, osteolysis, MESH: Female
Abstract

International audience; This study prospectively assessed the outcome of 134 cemented titanium stems and serum ion levels. The stems were polished (0.1 microm Ra) with circular cross section. At the end point, only one stem revision was performed for aseptic loosening, and two were planned due to subsidence greater than 5 mm. Non-progressive radiolucencies in zones 1 and 7 were observed in 16 hips at the cement-bone interface without osteolysis. Median serum titanium concentrations were below the detection limit (30 nmol/l) except in patients with failed stems. The overall stem survival rate was 97.7% at nine years, which is comparable to other series of cemented stems. The protective layer of titanium oxide coating the stem and a thick cement mantle may help resist aseptic loosening. In addition, satisfactory monitoring of the stem was reached using titanium serum level determination.

Published
2009
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11. Individual blood-cell capture and 2D organization on microarrays.: Individual Blood Cell Capture and 2D-Organization on Microarrays

Author

Roupioz, Yoann, Berthet-Duroure, Nathalie, Leichle, Thierry, Pourciel, Jean Bernard, Mailley, Pascal, Cortes, Sandra, Villiers, Marie-Bernadette, Marche, Patrice, Livache, Thierry, Nicu, Liviu, Structures et propriétés d'architectures moléculaire (SPRAM - UMR 5819), Institut Nanosciences et Cryogénie (INAC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Laboratoire d'analyse et d'architecture des systèmes (LAAS), Université Toulouse Capitole (UT Capitole), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université Toulouse - Jean Jaurès (UT2J), Université de Toulouse (UT)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT), Institut d'oncologie/développement Albert Bonniot de Grenoble (INSERM U823), Université Joseph Fourier - Grenoble 1 (UJF)-CHU Grenoble-EFS-Institut National de la Santé et de la Recherche Médicale (INSERM), INSERM U823, équipe 8 (Immunologie Analytique des Pathologies Chroniques), Université Joseph Fourier - Grenoble 1 (UJF)-CHU Grenoble-EFS-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Joseph Fourier - Grenoble 1 (UJF)-CHU Grenoble-EFS-Institut National de la Santé et de la Recherche Médicale (INSERM), This work was partly supported by the French Nanobio Funding program., Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Université Toulouse - Jean Jaurès (UT2J)-Université Toulouse 1 Capitole (UT1), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées, Institut National de la Santé et de la Recherche Médicale (INSERM)-EFS-CHU Grenoble-Université Joseph Fourier - Grenoble 1 (UJF), Institut National de la Santé et de la Recherche Médicale (INSERM)-EFS-CHU Grenoble-Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM)-EFS-CHU Grenoble-Université Joseph Fourier - Grenoble 1 (UJF), Marche, Patrice, Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université Fédérale Toulouse Midi-Pyrénées-Institut National des Sciences Appliquées (INSA)-Université Toulouse - Jean Jaurès (UT2J)-Université Toulouse III - Paul Sabatier (UT3), and Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP)

Subjects
Blood cells, MESH: Humans, [SDV.IMM] Life Sciences [q-bio]/Immunology, MESH: Protein Array Analysis, [SPI.NANO] Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics, MESH: Blood Cells, micro-cantilever, surface patterning, MESH: Equipment Failure Analysis, MESH: Flow Cytometry, cell microarray, MESH: Cell Separation, MESH: Sensitivity and Specificity, MESH: Reproducibility of Results, electrochemistry, MESH: Microfluidic Analytical Techniques, MESH: Blood Proteins, [SDV.IMM]Life Sciences [q-bio]/Immunology, [SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics, MESH: Nanotechnology, ComputingMilieux_MISCELLANEOUS, MESH: Cells, Cultured, MESH: Equipment Design
Abstract

International audience

Published
2009
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12. Effects of laser beam propagation and saturation on the spatial shape of sodium laser guide stars

Author

Hugues Guillet de Chatellus, Fabien Marc, Jean-Paul Pique, Laboratoire de l'intégration, du matériau au système (IMS), Université Sciences et Technologies - Bordeaux 1-Institut Polytechnique de Bordeaux-Centre National de la Recherche Scientifique (CNRS), LIPhy-OPTIMA, Laboratoire Interdisciplinaire de Physique [Saint Martin d’Hères] (LIPhy), Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF)-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF), Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF), LIPhy-OLA, and LIPhy-CLOE

Subjects
Light, Astronomy, Physics::Optics, MESH: Equipment Failure Analysis, 02 engineering and technology, 01 natural sciences, law.invention, Stars, Celestial, law, Scattering, Radiation, Astrophysics::Solar and Stellar Astrophysics, MESH: Sodium, Laser power scaling, MESH: Lenses, Lenses, Physics, MESH: Stars, Celestial, [PHYS.PHYS.PHYS-OPTICS]Physics [physics]/Physics [physics]/Optics [physics.optics], Astrophysics::Instrumentation and Methods for Astrophysics, Sodium layer, Equipment Design, 021001 nanoscience & nanotechnology, Atomic and Molecular Physics, and Optics, MESH: Reproducibility of Results, Computer-Aided Design, MESH: Lasers, Guide star, Laser beam quality, 0210 nano-technology, MESH: Refractometry, Beam parameter product, Sensitivity and Specificity, 010309 optics, Optics, 0103 physical sciences, MESH: Scattering, Radiation, Adaptive optics, business.industry, Lasers, Sodium, Reproducibility of Results, MESH: Computer-Aided Design, MESH: Astronomy, [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, Wavefront sensor, Laser, MESH: Sensitivity and Specificity, MESH: Light, Equipment Failure Analysis, Refractometry, business, MESH: Equipment Design
Abstract

International audience; The possibility to produce diffraction-limited images by large telescopes through Adaptive Optics is closely linked to the precision of measurement of the position of the guide star on the wavefront sensor. In the case of laser guide stars, many parameters can lead to a strong distortion on the shape of the LGS spot. Here we study the influence of both the saturation of the sodium layer excited by different types of lasers, the spatial quality of the laser mode at the ground and the influence of the atmospheric turbulence on the upward propagation of the laser beam. Both shape and intensity of the LGS spot are found to depend strongly on these three effects with important consequences on the precision on the wavefront analysis.

Published
2009
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13. Electron-multiplying charge-coupled detector-based bioluminescence recording of single-cell Ca2+

Author

Marie-Anne Nicola, Sandrine Picaud, Philippe Brulet, Olivier Renaud, Kelly L. Rogers, Spencer L. Shorte, Jean-René Martin, Marie Nguyen, Eric Karplus, Imagerie Dynamique (Plate-Forme) (PFID), Institut Pasteur [Paris], Laboratoire de neurobiologie cellulaire et moléculaire (NBCM), Centre National de la Recherche Scientifique (CNRS), Science Wares Incorporated, Embryologie Moléculaire, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Institut Pasteur [Paris] (IP), and Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Transducers, Biomedical Engineering, Aequorin, Molecular Probe Techniques, MESH: Equipment Failure Analysis, Nanotechnology, Electrons, MESH: Calcium Signaling, Green fluorescent protein, Biomaterials, 03 medical and health sciences, 0302 clinical medicine, Bioluminescence, Calcium Signaling, 030304 developmental biology, Luminescent Proteins, 0303 health sciences, MESH: Electrons, biology, MESH: Aequorin, Chemistry, Molecular biophysics, Detector, Equipment Design, biology.organism_classification, Fluorescence, Atomic and Molecular Physics, and Optics, Electronic, Optical and Magnetic Materials, Equipment Failure Analysis, MESH: Molecular Probe Techniques, MESH: Luminescent Measurements, MESH: Calcium, Luminescent Measurements, Biophysics, biology.protein, Aequorea victoria, Calcium, MESH: Luminescent Proteins, [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], 030217 neurology & neurosurgery, MESH: Equipment Design, MESH: Transducers
Abstract

The construction and application of genetically encoded intracellular calcium concentration ([Ca2+]i) indicators has a checkered history. Excitement raised over the creation of new probes is often followed by disappointment when it is found that the initial demonstrations of [Ca2+]i sensing capability cannot be leveraged into real scientific advances. Recombinant apo-aequorin cloned from Aequorea victoria was the first Ca2+ sensitive protein genetically targeted to subcellular compartments. In the jellyfish, bioluminescence resonance energy transfer (BRET) between Ca2+ bound aequorin and green fluorescent protein (GFP) emits green light. Similarly, Ca2+ sensitive bioluminescent reporters undergoing BRET have been constructed between aequorin and GFP, and more recently with other fluorescent protein variants. These hybrid proteins display red-shifted spectrums and have higher light intensities and stability compared to aequorin alone. We report BRET measurement of single-cell [Ca2+]i based on the use of electron-multiplying charge-coupled-detector (EMCCD) imaging camera technology, mounted on either a bioluminescence or conventional microscope. Our results show for the first time how these new technologies make facile long-term monitoring of [Ca2+]i at the single-cell level, obviating the need for expensive, fragile, and sophisticated equipment based on image-photon-detectors (IPD) that were until now the only technical recourse to dynamic BRET experiments of this type.

Published
2008
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14. In vivo mice lung tumor follow-up with fluorescence diffuse optical tomography

Author

Philippe Rizo, Jean-Marc Dinten, Anabela Da Silva, Jean-Luc Coll, Lionel Hervé, Anne Koenig, Philippe Peltie, Jérôme Boutet, Véronique Josserand, Michel Berger, Commissariat à l'énergie atomique et aux énergies alternatives - Laboratoire d'Electronique et de Technologie de l'Information (CEA-LETI), Direction de Recherche Technologique (CEA) (DRT (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Institut d'oncologie/développement Albert Bonniot de Grenoble (INSERM U823), Institut National de la Santé et de la Recherche Médicale (INSERM)-EFS-CHU Grenoble-Université Joseph Fourier - Grenoble 1 (UJF), INSERM U823, équipe 5 (cibles diagnostiques ou thérapeutiques et vectorisation de drogues dans le cancer du poumon), Institut National de la Santé et de la Recherche Médicale (INSERM)-EFS-CHU Grenoble-Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM)-EFS-CHU Grenoble-Université Joseph Fourier - Grenoble 1 (UJF), Université Joseph Fourier - Grenoble 1 (UJF)-CHU Grenoble-EFS-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Joseph Fourier - Grenoble 1 (UJF)-CHU Grenoble-EFS-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Joseph Fourier - Grenoble 1 (UJF)-CHU Grenoble-EFS-Institut National de la Santé et de la Recherche Médicale (INSERM), and Hurbin, Amandine

Subjects
Pathology, medicine.medical_specialty, MESH: Cell Line, Tumor, MESH: Mammary Neoplasms, Experimental, Biomedical Engineering, MESH: Equipment Failure Analysis, Mice, Nude, MESH: Microscopy, Fluorescence, [SDV.CAN]Life Sciences [q-bio]/Cancer, 01 natural sciences, Sensitivity and Specificity, Imaging phantom, 030218 nuclear medicine & medical imaging, 010309 optics, Biomaterials, 03 medical and health sciences, Mice, 0302 clinical medicine, [SDV.CAN] Life Sciences [q-bio]/Cancer, MESH: Tomography, Optical, In vivo, Cell Line, Tumor, 0103 physical sciences, MESH: Mice, Nude, Medical imaging, medicine, Animals, Tomography, Optical, MESH: Animals, MESH: Mice, Chemistry, Mammary Neoplasms, Experimental, Equipment Design, Image Enhancement, Fluorescence, MESH: Sensitivity and Specificity, Atomic and Molecular Physics, and Optics, Diffuse optical imaging, Electronic, Optical and Magnetic Materials, Equipment Failure Analysis, Microscopy, Fluorescence, Body region, Female, Tomography, MESH: Image Enhancement, MESH: Female, Preclinical imaging, MESH: Equipment Design
Abstract

International audience; We present in vivo experiments conducted with a new fluorescence diffuse optical tomographic (fDOT) system on cancerous mice bearing mammary murine tumors. We first briefly present this new system that has been developed and its associated reconstruction method. Its main specificity is its ability to reconstruct the fluorescence yield even in heterogeneous and highly attenuating body regions such as lungs and to enable mouse inspection without immersion in optical index matching liquid (Intralipid and ink). Some phantom experiments validate the performance of this new system for heterogeneous media inspection. Its use for a mice study is then related. It consists in the follow-up of the lungs at different stages of tumor development after injection of RAFT-(cRGD)4-Alexa700. As expected, the reconstructed fluorescence increases along with the tumor stage. These results validate the use of our system for biological studies of small animals.

Published
2008
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15. Pressure sensor-based tongue-placed electrotactile biofeedback for balance improvement - Biomedical application to prevent pressure sores formation and falls

Author

Yohan Payan, Anthony Fleury, Olivier Chenu, Nicolas Pinsault, Jacques Demongeot, Nicolas Vuillerme, Alexandre Moreau-Gaudry, Techniques de l'Ingénierie Médicale et de la Complexité - Informatique, Mathématiques et Applications, Grenoble - UMR 5525 (TIMC-IMAG), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF), AGeing and IMagery (AGIM), Centre National de la Recherche Scientifique (CNRS)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Joseph Fourier - Grenoble 1 (UJF)-Université Pierre Mendès France - Grenoble 2 (UPMF), GMCAO, VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF)-Centre d'Investigation Clinique - Innovation Technologique - INSERM - CHU de Grenoble (CIC-IT Grenoble (CIT803)), CHU Grenoble-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Grenoble Alpes (UGA)-CHU Grenoble-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Grenoble Alpes (UGA), Gestes Medico-chirurgicaux Assistés par Ordinateur (TIMC-IMAG-GMCAO), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF), Université Joseph Fourier - Grenoble 1 (UJF)-Centre National de la Recherche Scientifique (CNRS)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS), and Université Joseph Fourier - Grenoble 1 (UJF)-Centre National de la Recherche Scientifique (CNRS)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Université Joseph Fourier - Grenoble 1 (UJF)-Centre National de la Recherche Scientifique (CNRS)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)

Subjects
[SPI.OTHER]Engineering Sciences [physics]/Other, 030506 rehabilitation, Pressure sores, medicine.medical_treatment, Handicap, MESH: Equipment Failure Analysis, User-Computer Interface, 0302 clinical medicine, Fall, Telemetry, Medicine, Spinal cord injury, Tetraplegia, MESH: Biofeedback, Psychology, Pressure Ulcer, MESH: Tongue, MESH: Manometry, [SPI.MECA.BIOM]Engineering Sciences [physics]/Mechanics [physics.med-ph]/Biomechanics [physics.med-ph], MESH: Posture, Equipment Design, Tactile display, MESH: Accidental Falls, 3. Good health, Sensory substitution, Neurons and Cognition (q-bio.NC), [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], [SDV.IB]Life Sciences [q-bio]/Bioengineering, MESH: Self-Help Devices, 0305 other medical science, Paraplegia, Biomedical engineering, medicine.medical_specialty, MESH: Pressure Ulcer, Manometry, Posture, FOS: Physical sciences, Biofeedback, [SPI.AUTO]Engineering Sciences [physics]/Automatic, 03 medical and health sciences, Physical medicine and rehabilitation, Tongue, Physical Stimulation, Humans, MESH: Physical Stimulation, MESH: User-Computer Interface, Balance (ability), MESH: Telemetry, MESH: Humans, business.industry, [SCCO.NEUR]Cognitive science/Neuroscience, Postural control, Biofeedback, Psychology, Self-Help Devices, medicine.disease, Pressure sensor, Physics - Medical Physics, Equipment Failure Analysis, FOS: Biological sciences, Quantitative Biology - Neurons and Cognition, Physical therapy, ComputingMilieux_COMPUTERSANDSOCIETY, Accidental Falls, [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie, Medical Physics (physics.med-ph), business, 030217 neurology & neurosurgery, Tactile sensor, MESH: Equipment Design
Abstract

International audience; We introduce the innovative technologies, based on the concept of "sensory substitution", we are developing in the fields of biomedical engineering and human disability. Precisely, our goal is to design, develop and validate practical assistive biomedical and/or technical devices and/or rehabilitating procedures for persons with disabilities, using artificial tongue-placed tactile biofeedback systems. Proposed applications are dealing with: (1) pressure sores prevention in case of spinal cord injuries (persons with paraplegia, or tetraplegia); and (2) balance control improvement to prevent fall in older and/or disabled adults. This paper describes the architecture and the functioning principle of these biofeedback systems and presents preliminary results of two feasibility studies performed on young healthy adults.

Published
2007
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16. MRS quality assessment in a multicentre study on MRS-based classification of brain tumours

Author

Arend Heerschap, Àngel Moreno-Torres, Yvonne M. van der Meulen, Kirstie S. Opstad, Anne Ziegler, Carles Majós, Mark Rijpkema, Marinette van der Graaf, Margarida Julià-Sapé, Carles Arús, Dionisio Acosta, Franklyn A. Howe, Department of Radiology, Radboud University Medical Center [Nijmegen], Departament de Bioquímica i Biologia Molecular, Universitat Autònoma de Barcelona (UAB), Department of Basic Medical Sciences, Queen Mary University of London (QMUL), Neuroimagerie Fonctionnelle et Metabolique, Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut de Diagnòstic per la Imatge, CSU de Bellvitge, Centre Diagnòstic Pedralbes, European Union, Grant Number: IST 1999-10310, and Dojat, Michel

Subjects
Magnetic Resonance Spectroscopy, Databases, Factual, Computer science, [SDV.IB.IMA]Life Sciences [q-bio]/Bioengineering/Imaging, MESH: Quality Control, MESH: Equipment Failure Analysis, computer.software_genre, 030218 nuclear medicine & medical imaging, Pattern Recognition, Automated, MESH: Clinical Protocols, 0302 clinical medicine, Data acquisition, Clinical Protocols, Voxel, MESH: Pattern Recognition, Automated, Multicenter Studies as Topic, Spectroscopy, media_common, MESH: Program Evaluation, medicine.diagnostic_test, Quality assessment, Brain Neoplasms, Phantoms, Imaging, phantom, Nutrition and Health [UMCN 5.5], Reference Standards, multicentre study, Visual inspection, MESH: Reproducibility of Results, MESH: Brain Neoplasms, Molecular Medicine, MESH: Reference Standards, MR spectra, Protons, Quality Control, system quality assurance, quality assessment, [SDV.CAN]Life Sciences [q-bio]/Cancer, Expert Systems, Imaging phantom, 03 medical and health sciences, MESH: Software, [SDV.CAN] Life Sciences [q-bio]/Cancer, medicine, MESH: Water, MESH: European Union, media_common.cataloged_instance, Humans, Radiology, Nuclear Medicine and imaging, European Union, European union, MESH: Humans, business.industry, MESH: Expert Systems, MESH: Magnetic Resonance Spectroscopy, Reproducibility of Results, Water, Magnetic resonance imaging, MESH: Databases, Factual, Equipment Failure Analysis, [SDV.IB.IMA] Life Sciences [q-bio]/Bioengineering/Imaging, System quality, MESH: Phantoms, Imaging, MESH: Multicenter Studies as Topic, H MRS, MESH: Protons, Functional Imaging [UMCN 1.1], Nuclear medicine, business, computer, 030217 neurology & neurosurgery, Software, Program Evaluation
Abstract

Contains fulltext : 69096.pdf (Publisher’s version ) (Closed access) This paper reports on quality assessment of MRS in the European Union-funded multicentre project INTERPRET (International Network for Pattern Recognition of Tumours Using Magnetic Resonance; http://azizu.uab.es/INTERPRET), which has developed brain tumour classification software using in vivo proton MR spectra. The quality assessment consisted of both MR system quality assurance (SQA) and quality control (QC) of spectral data acquired from patients and healthy volunteers. The system performance of the MR spectrometers at all participating centres was checked bimonthly by a short measurement protocol using a specially designed INTERPRET phantom. In addition, a more extended SQA protocol was performed yearly and after each hardware or software upgrade. To compare the system performance for in vivo measurements, each centre acquired MR spectra from the brain of five healthy volunteers. All MR systems fulfilled generally accepted minimal system performance for brain MRS during the entire data acquisition period. The QC procedure of the MR spectra in the database comprised automatic determination of the signal-to-noise ratio (SNR) in a water-suppressed spectrum and of the line width of the water resonance (water band width, WBW) in the corresponding non-suppressed spectrum. Values of SNR > 10 and WBW < 8 Hz at 1.5 T were determined empirically as conservative threshold levels required for spectra to be of acceptable quality. These thresholds only hold for SNR and WBW values using the definitions and data processing described in this article. A final QC check consisted of visual inspection of each clinically validated water-suppressed metabolite spectrum by two, or, in the case of disagreement, three, experienced MR spectroscopists, to detect artefacts such as large baseline distortions, exceptionally broadened metabolite peaks, insufficient removal of the water line, large phase errors, and signals originating from outside the voxel. In the end, 10% of 889 spectra with completed spectroscopic judgement were discarded.

Published
2007
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17. Skin Radiance, how to quantify ? Validation of an Optical method

Author

Philippe Humbert, Tijani Gharbi, Brigitte Closs, S. Mac-Mary, Adeline Petitjean, Patrice Muret, J M Sainthillier, Institut de Science et d'ingénierie supramoléculaires (ISIS), Université Louis Pasteur - Strasbourg I-Centre National de la Recherche Scientifique (CNRS), Franche-Comté Électronique Mécanique, Thermique et Optique - Sciences et Technologies (UMR 6174) (FEMTO-ST), Université de Franche-Comté (UFC)-Centre National de la Recherche Scientifique (CNRS)-Ecole Nationale Supérieure de Mécanique et des Microtechniques (ENSMM)-Université de Technologie de Belfort-Montbeliard (UTBM), Université Libre de Bruxelles [Bruxelles] (ULB), Saas, Philippe, Laboratoire d'Ingénierie et Biologie Cutanées (LIBC), IFR33-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC), R&D Department, SILAB, Interactions hôte-greffon-tumeur, ingénierie cellulaire et génique - UFC (UMR INSERM 1098) (RIGHT), Institut National de la Santé et de la Recherche Médicale (INSERM)-Etablissement français du sang [Bourgogne-Franche-Comté] (EFS [Bourgogne-Franche-Comté])-Université de Franche-Comté (UFC), Université de Technologie de Belfort-Montbeliard (UTBM)-Ecole Nationale Supérieure de Mécanique et des Microtechniques (ENSMM)-Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC), Université de Technologie de Belfort-Montbeliard (UTBM)-Ecole Nationale Supérieure de Mécanique et des Microtechniques (ENSMM)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Centre National de la Recherche Scientifique (CNRS), Université Louis Pasteur - Strasbourg I-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), and Université libre de Bruxelles (ULB)

Subjects
Adult, Validation study, MESH: Refractometry, [SDV.IMM] Life Sciences [q-bio]/Immunology, MESH: Equipment Failure Analysis, Skin Pigmentation, MESH: Algorithms, Dermatology, Sensitivity and Specificity, Photometry, 030207 dermatology & venereal diseases, 03 medical and health sciences, Complexion Radiance, 0302 clinical medicine, Optics, Brightness, MESH: Colorimetry, Validation, medicine, Humans, Mathematics, Skin, Reproducibility, MESH: Humans, MESH: Middle Aged, business.industry, Reproducibility of Results, Complexion, MESH: Adult, Equipment Design, Repeatability, Middle Aged, MESH: Photometry, MESH: Sensitivity and Specificity, Equipment Failure Analysis, MESH: Reproducibility of Results, Refractometry, medicine.anatomical_structure, 030220 oncology & carcinogenesis, Forehead, Radiance, [SPI.OPTI]Engineering Sciences [physics]/Optics / Photonic, [SDV.IMM]Life Sciences [q-bio]/Immunology, Colorimetry, MESH: Skin Pigmentation, Reflective Light, business, Algorithms, MESH: Equipment Design
Abstract

International audience; BACKGROUND/PURPOSE: The aim of this study was to validate an optic device used to measure the quantity of light reflected by the skin, which depends on the brightness of the skin. METHODS: This apparatus gives three parameters, called Complexion/diffusion (Cd), Cr (Complexion/reflection) and Complexion/specular position (Csp). Measurements were carried out on the forehead (slightly rounded surface) and the cheekbone (angular surface). To estimate the repeatability and the reproducibility of each parameter, two female subjects (one with a dull complexion and the other with a glowing complexion) and three operators participated in the study. To investigate repeatability, each operator performed 10 consecutive measurements on both zones. To investigate inter-investigator reproducibility, the operators performed the measurements alternately. To investigate day-to-day reproducibility, these measures were achieved during three visits at a 2-day interval, at D1, D3 and D5. To estimate the sensitivity and the specificity, 30 females participated in the study (15 in the dull complexion group and 15 in the glowing complexion group). RESULTS: Cd was repeatable (variation coefficient 0.05) but not inter-investigators reproducible (generally P0.05) and not inter-investigators reproducible (generally P5%) on both the studied zones and for the three investigators. CONCLUSION: The repeatability and the day-to-day reproducibility were satisfying for the Cd and Cr parameters on both the studied sites. The sensitivity and the specificity were satisfying for both parameters on both sites, except for the specificity of the Cd parameter on the cheekbone. Therefore, this apparatus is suitable to follow-up an evolution on both the studied sites, but we recommend: (a) that the same trained investigator always performs the measurements, (b) to privilege the forehead in particular for a classification (dull or glowing).

Published
2006
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18. Usefulness of an integrated microfluidic device (HPLC-Chip-MS) to enhance confidence in protein identification by proteomics

Author

Magalie Duchateau, Julie Hardouin, Michel Caron, Raymonde Joubert-Caron, Laboratoire de Biophysique Moléculaire Cellulaire et Tissulaire (BIOMOCETI), and Université Paris 13 (UP13)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Proteomics, Spectrometry, Mass, Electrospray Ionization, Proteome, Microfluidics, MESH: Equipment Failure Analysis, Mass spectrometry, Peptide Mapping, Sensitivity and Specificity, 01 natural sciences, MESH: Spectrometry, Mass, Electrospray Ionization, MESH: Peptide Mapping, Analytical Chemistry, 03 medical and health sciences, Nanotechnology, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, Biomarker discovery, MESH: Chromatography, High Pressure Liquid, MESH: Nanotechnology, Chromatography, High Pressure Liquid, Spectroscopy, 030304 developmental biology, Gel electrophoresis, 0303 health sciences, Chromatography, Chemistry, MESH: Proteomics, 010401 analytical chemistry, Organic Chemistry, Reproducibility of Results, Equipment Design, Microfluidic Analytical Techniques, MESH: Sensitivity and Specificity, 0104 chemical sciences, Equipment Failure Analysis, Systems Integration, MESH: Reproducibility of Results, MESH: Proteome, MESH: Microfluidic Analytical Techniques, Protein identification, Hplc chip, Ion trap, MESH: Systems Integration, MESH: Equipment Design
Abstract

International audience; Nanoflow liquid chromatography/mass spectrometry (nanoLC/MS) has become a current tool in proteomics applications increasingly used in the search for new biomarkers. A new integrated microfluidic device (HPLC-Chip), coupled to ion trap mass spectrometry (ITMS), appears as an innovative and robust tool for improving the identifications commonly performed by nanoLC/MS/MS. We tested this device for the identification of proteins obtained from two-dimensional gel electrophoresis or chromatography. The chip allows the measurement of reproducible retention times that, in association with m/z ratios, was found useful for identifying peptide sequences without ambiguity. A sensitivity increase of a factor of at least 5-fold is obtained compared to the results obtained previously in our laboratory by conventional nanoLC/MS/MS on the same ion trap. We conclude that this recently available microfluidic device can be a valuable tool during biomarker discovery programs, particularly identifying low-abundance proteins.

Published
2006
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19. Real time electroporation control for accurate and safe in vivo non-viral gene therapy

Author

Lluis M. Mir, Damijan Miklavčič, D. Cukjati, Franck M. Andre, Danute Batiuskaite, Vectorologie et transfert de gènes (VTG / UMR8121), Université Paris-Sud - Paris 11 (UP11)-Institut Gustave Roussy (IGR)-Centre National de la Recherche Scientifique (CNRS), Faculty of Electrical Engineering, University of Ljubljana, Faculty of Natural Sciences - Department of Biology, and Vytautas Magnus University - Vytauto Didziojo Universitetas (VDU)

Subjects
Electrochemotherapy, MESH: Microelectrodes, Genetic enhancement, Cell, MESH: Equipment Failure Analysis, 0302 clinical medicine, Electrochemistry, MESH: Animals, 0303 health sciences, MESH: Muscle, Skeletal, MESH: Feedback, Electroporation, MESH: DNA, General Medicine, Transfection, Equipment Design, Cell biology, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biomolecules [q-bio.BM], medicine.anatomical_structure, Liver, Naked DNA, 030220 oncology & carcinogenesis, Viruses, Female, MESH: Rats, Biophysics, Biology, Sensitivity and Specificity, Feedback, MESH: Viruses, 03 medical and health sciences, In vivo, Computer Systems, medicine, Animals, Luciferase, MESH: Electroporation, Physical and Theoretical Chemistry, Rats, Wistar, Muscle, Skeletal, 030304 developmental biology, MESH: Transfection, MESH: Computer Systems, MESH: Rats, Wistar, DNA, Genetic Therapy, Molecular biology, MESH: Sensitivity and Specificity, Rats, Equipment Failure Analysis, MESH: Gene Therapy, MESH: Female, Microelectrodes, MESH: Equipment Design, MESH: Liver
Abstract

In vivo cell electroporation is the basis of DNA electrotransfer, an efficient method for non-viral gene therapy using naked DNA. The electric pulses have two roles, to permeabilize the target cell plasma membrane and to transport the DNA towards or across the permeabilized membrane by electrophoresis. For efficient electrotransfer, reversible undamaging target cell permeabilization is mandatory. We report the possibility to monitor in vivo cell electroporation during pulse delivery, and to adjust the electric field strength on real time, within a few microseconds after the beginning of the pulse, to ensure efficacy and safety of the procedure. A control algorithm was elaborated, implemented in a prototype device and tested in luciferase gene electrotransfer to mice muscles. Controlled pulses resulted in protection of the tissue and high levels of luciferase in gene transfer experiments where uncorrected excessive applied voltages lead to intense muscle damage and consecutive loss of luciferase gene expression.

Published
2006
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20. Accuracy of 16-detector multislice spiral computed tomography in the initial evaluation of dilated cardiomyopathy

Author

Martine Gilard, Ulric Vinsonneau, Grégoire Le Gal, Jacques Boschat, Pierre-Yves Pennec, Jean-Jacques Blanc, Jean-Christophe Cornily, Jacques Mansourati, Département de Cardiologie (CHRU - Cardio), Centre Hospitalier Régional Universitaire de Brest (CHRU Brest), Service d'Hémodynamique, Centre Hospitalier Régional Universitaire de Brest (CHRU Brest)-Hôpital de la Cavale Blanche - CHRU Brest (CHU - BREST ), Optimisation des régulations physiologiques (ORPHY (EA 4324)), Institut Brestois Santé Agro Matière (IBSAM), Université de Brest (UBO)-Université de Brest (UBO)-Université de Brest (UBO)-Centre Hospitalier Régional Universitaire de Brest (CHRU Brest), Centre d'Investigation Clinique (CIC - Brest), Université de Brest (UBO)-Institut National de la Santé et de la Recherche Médicale (INSERM), Département de Cardiologie (HIA - Cardio), and Hôpital d'Instruction des armées

Subjects
Male, MESH: Equipment Failure Analysis, Pilot Projects, 030204 cardiovascular system & hematology, Coronary Angiography, 030218 nuclear medicine & medical imaging, Coronary artery disease, 0302 clinical medicine, MESH: Middle Aged, medicine.diagnostic_test, Dilated cardiomyopathy, General Medicine, Equipment Design, Middle Aged, Spiral computed tomography, MESH: Reproducibility of Results, Radiographic Image Enhancement, 030220 oncology & carcinogenesis, End-diastolic volume, Radiographic Image Interpretation, Computer-Assisted, Female, Radiology, Agatston score, MESH: Transducers, Cardiomyopathy, Dilated, medicine.medical_specialty, Transducers, Sensitivity and Specificity, 03 medical and health sciences, medicine, MESH: Radiographic Image Interpretation, Computer-Assisted, Humans, Multislice, Radiology, Nuclear Medicine and imaging, cardiovascular diseases, MESH: Cardiomyopathy, Dilated, MESH: Humans, business.industry, Reproducibility of Results, medicine.disease, MESH: Pilot Projects, MESH: Coronary Angiography, MESH: Sensitivity and Specificity, MESH: Male, Equipment Failure Analysis, Heart failure, MESH: Radiographic Image Enhancement, Angiography, MESH: Tomography, Spiral Computed, business, MESH: Female, Tomography, Spiral Computed, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology, MESH: Equipment Design
Abstract

International audience; BACKGROUND: Multislice Computed Tomography (MSCT) recently proved its accuracy in the detection of coronary artery disease (CAD). It can also give information about left ventricular function and venous network anatomy. We here sought to validate a MSCT-based strategy in the initial evaluation of patients with dilated cardiomyopathy (DCM). METHODS: 36 patients with DCM underwent cardiac MSCT before conventional coronary angiography with ventriculography. We analysed arterial calcium score (Agatston score equivalent: ASE), coronary stenosis, left ventricular parameters and venous network. RESULTS: The sensitivity of a MSCT-based strategy in detecting significant CAD was 100% and the specificity 80%. The positive and negative predictive values were respectively 67% and 100%. For ASE or =1000, MSCT enabled conventional angiography to be avoided in only 2/9 patients (22.2%). The ventricle was assessable in 83.4% (30 patients) on MSCT. Correlation coefficient Rs with ventriculography were 0.78 (p1.000, conventional coronary angiography is mandatory due to MSCT's poor interest in such cases; when ASE

Published
2006
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21. Fourier-transform coherent anti-Stokes Raman scattering microscopy

Author

Antigoni Alexandrou, Emmanuel Beaurepaire, Manuel Joffre, Jennifer P. Ogilvie, Laboratoire d'optique et biosciences (LOB), and École polytechnique (X)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Microscope, Light, [SDV.IB.IMA]Life Sciences [q-bio]/Bioengineering/Imaging, MESH: Equipment Failure Analysis, Information Storage and Retrieval, 02 engineering and technology, MESH: Signal Processing, Computer-Assisted, Spectrum Analysis, Raman, 01 natural sciences, law.invention, law, Microscopy, Scattering, Radiation, [PHYS.PHYS.PHYS-OPTICS]Physics [physics]/Physics [physics]/Optics [physics.optics], Fourier Analysis, Phantoms, Imaging, Signal Processing, Computer-Assisted, Equipment Design, 021001 nanoscience & nanotechnology, Atomic and Molecular Physics, and Optics, MESH: Tomography, Optical Coherence, symbols, MESH: Image Enhancement, 0210 nano-technology, Raman scattering, Algorithms, Tomography, Optical Coherence, Materials science, MESH: Algorithms, 010309 optics, symbols.namesake, Optics, 0103 physical sciences, Image Interpretation, Computer-Assisted, Microscopy, Interference, Coherent anti-Stokes Raman spectroscopy, MESH: Scattering, Radiation, MESH: Microscopy, Interference, MESH: Spectrum Analysis, Raman, business.industry, Michelson interferometer, MESH: Information Storage and Retrieval, Laser, Image Enhancement, MESH: Light, Equipment Failure Analysis, MESH: Phantoms, Imaging, Microscopy, Fluorescence, Multiphoton, MESH: Microscopy, Fluorescence, Multiphoton, business, Raman spectroscopy, Ultrashort pulse, MESH: Image Interpretation, Computer-Assisted, MESH: Fourier Analysis, MESH: Equipment Design
Abstract

International audience; We report a novel Fourier-transform-based implementation of coherent anti-Stokes Raman scattering (CARS) microscopy. The method employs a single femtosecond laser source and a Michelson interferometer to create two pulse replicas that are fed into a scanning multiphoton microscope. By varying the time delay between the pulses, we time-resolve the CARS signal, permitting easy removal of the nonresonant background while providing high resolution, spectrally resolved images of CARS modes over the laser bandwidth (approximately 1500 cm(-1)). We demonstrate the method by imaging polystyrene beads in solvent.

Published
2006
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22. Study of osteoblastic cells in a microfluidic environment

Author

Eric Leclerc, Pierre Layrolle, B. David, Teruo Fujii, Laurent Griscom, Bruno LePioufle, C. Legallaisa, Biomécanique et génie biomédical (BIM), Centre National de la Recherche Scientifique (CNRS), Laboratoire d'ingénierie osteo-articulaire et dentaire (LIOAD), Université de Nantes (UN)-IFR26-Institut National de la Santé et de la Recherche Médicale (INSERM), Systèmes et Applications des Technologies de l'Information et de l'Energie (SATIE), École normale supérieure - Cachan (ENS Cachan)-Université Paris-Sud - Paris 11 (UP11)-Institut Français des Sciences et Technologies des Transports, de l'Aménagement et des Réseaux (IFSTTAR)-École normale supérieure - Rennes (ENS Rennes)-Université de Cergy Pontoise (UCP), Université Paris-Seine-Université Paris-Seine-Conservatoire National des Arts et Métiers [CNAM] (CNAM)-Centre National de la Recherche Scientifique (CNRS), Bio-MIcroSystèmes et BioSensors (SATIE-BIOMIS), Systèmes d'Information et d'Analyse Multi-Echelles (SIAME), Université Paris-Seine-Université Paris-Seine-Conservatoire National des Arts et Métiers [CNAM] (CNAM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Cachan (ENS Cachan)-Université Paris-Sud - Paris 11 (UP11)-Institut Français des Sciences et Technologies des Transports, de l'Aménagement et des Réseaux (IFSTTAR)-École normale supérieure - Rennes (ENS Rennes)-Université de Cergy Pontoise (UCP), Université Paris-Seine-Université Paris-Seine-Conservatoire National des Arts et Métiers [CNAM] (CNAM)-Centre National de la Recherche Scientifique (CNRS)-Systèmes et Applications des Technologies de l'Information et de l'Energie (SATIE), Laboratory for Integrated Micro Mechatronics Systems (LIMMS), Centre National de la Recherche Scientifique (CNRS)-The University of Tokyo (UTokyo), Physiopathologie des Adaptations Nutritionnelles (PhAN), Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Centre de Recherche sur les Matériaux d'Intérêt Biologique, Université de Nantes (UN)-UPRES EA 2159, Biomécanique et Bioingénierie (BMBI), Université de Technologie de Compiègne (UTC)-Centre National de la Recherche Scientifique (CNRS), Le Pioufle, Bruno, Université Paris-Seine-Université Paris-Seine-Conservatoire National des Arts et Métiers [CNAM] (CNAM), HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-Centre National de la Recherche Scientifique (CNRS), Systèmes d'Information et d'Analyse Multi-Echelles (SATIE-SIAME), HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Cachan (ENS Cachan)-Université Paris-Sud - Paris 11 (UP11)-Institut Français des Sciences et Technologies des Transports, de l'Aménagement et des Réseaux (IFSTTAR)-École normale supérieure - Rennes (ENS Rennes)-Université de Cergy Pontoise (UCP), HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-Centre National de la Recherche Scientifique (CNRS)-Systèmes et Applications des Technologies de l'Information et de l'Energie (SATIE), The University of Tokyo (UTokyo)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Université de Nantes (UN), and David, Bertrand

Subjects
MESH: 3T3 Cells, [SDV]Life Sciences [q-bio], Microfluidics, Cell Culture Techniques, MESH: Equipment Failure Analysis, 02 engineering and technology, Mechanotransduction, Cellular, MESH: Tissue Engineering, MESH: Microfluidics, Mice, chemistry.chemical_compound, MESH: Bioreactors, Bioreactors, Bone cell, MESH: Animals, Mechanotransduction, ComputingMilieux_MISCELLANEOUS, MESH: Cell Size, 0303 health sciences, Osteoblast, 3T3 Cells, Equipment Design, 021001 nanoscience & nanotechnology, [SDV] Life Sciences [q-bio], medicine.anatomical_structure, MESH: Cell Survival, Mechanics of Materials, 0210 nano-technology, Materials science, Cell Survival, [SPI.NANO] Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics, Biophysics, Bioengineering, 3T3 cells, Biomaterials, 03 medical and health sciences, MESH: Cell Proliferation, medicine, Animals, Viability assay, [SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics, [SDV.IB.BIO]Life Sciences [q-bio]/Bioengineering/Biomaterials, MESH: Mice, Cell Proliferation, Cell Size, 030304 developmental biology, MESH: Osteoblasts, MESH: Cell Culture Techniques, Osteoblasts, Tissue Engineering, Polydimethylsiloxane, MESH: Mechanotransduction, Cellular, In vitro, [SDV.IB.BIO] Life Sciences [q-bio]/Bioengineering/Biomaterials, Equipment Failure Analysis, chemistry, Ceramics and Composites, Ex vivo, Biomedical engineering, MESH: Equipment Design
Abstract

International audience; Bone tissue engineering consists of culturing osteoblastic cells onto synthetic three-dimensional (3D) porous scaffolds. The organization of bone cells into 3D scaffolds is crucial for ex vivo tissue formation. Diffusional rates of nutrients could be greatly improved by perfusing media through the 3D microporous scaffolds. However, bone cells cultured in vitro are responsive to a variety of different mechanical signals including fluid flow and shear stresses. In this work, we attempt to study osteoblastic cells behaviour cultured within microdevices allowing continuous and homogenous feeding of cells. We have fabricated polydimethylsiloxane PDMS microdevices with a 3D microstructured channel network. Mouse calvarial osteoblastic cells MC3T3-E1 were seeded at 2x10(6)cells/ml and cultured into the microdevices under flow rates of 0, 5, 35 microl/min. Cells attached and proliferated well in the designed microdevices. Cell viability was found around 85% up to 1 to 2 weeks for shear stress value under 5 mPa. The alkaline phosphatase (ALP) activity was enhanced 3- and 7.5-fold inside the microdevices under static and dynamic flow of 5 microl/min as compared to flat static cultures in PDMS coated Petri dishes. Therefore, osteoblastic cells could be successfully cultured inside the microdevices under dynamic conditions and their ALP activity was enhanced. These results are promising for bone cell growth and differentiation as well as future tissue regeneration using larger 3D microfluidic microdevices.

Published
2006
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23. High power transcranial beam steering for ultrasonic brain therapy

Author

Mathieu Pernot, Jean-François Aubry, Jean-Louis Thomas, Mickael Tanter, Mathias Fink, Laboratoire Ondes et Acoustique (UMR 7587) (LOA), Université Paris Diderot - Paris 7 (UPD7)-Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS), Pernot, Mathieu, Groupe de Physique des Solides (GPS), and Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Materials science, Hot Temperature, Phased array, Ultrasonic Therapy, Beam steering, MESH: Brain Diseases, Transducers, MESH: Equipment Failure Analysis, Grating, MESH: Hot Temperature, Models, Biological, Article, 030218 nuclear medicine & medical imaging, 03 medical and health sciences, MESH: Brain, 0302 clinical medicine, Optics, MESH: Computer Simulation, Humans, Radiology, Nuclear Medicine and imaging, Computer Simulation, Center frequency, Radiometry, [SDV.IB] Life Sciences [q-bio]/Bioengineering, Brain Diseases, MESH: Humans, Radiological and Ultrasound Technology, Hydrophone, business.industry, MESH: Radiometry, MESH: Models, Biological, MESH: Computer-Aided Design, Brain, Equipment Design, MESH: Ultrasonic Therapy, Equipment Failure Analysis, Transducer, Liver, Computer-Aided Design, Ultrasonic sensor, [SDV.IB]Life Sciences [q-bio]/Bioengineering, Focus (optics), business, 030217 neurology & neurosurgery, MESH: Transducers, MESH: Equipment Design, MESH: Liver
Abstract

International audience; A sparse phased array is specially designed for non-invasive ultrasound transskull brain therapy. The array is made of 200 single elements corresponding to a new generation of high power transducers developed in collaboration with Imasonic (Besançon, France). Each element has a surface of 0.5 cm2 and works at 0.9 MHz central frequency with a maximum 20 W cm(-2) intensity on the transducer surface. In order to optimize the steering capabilities of the array, several transducer distributions on a spherical surface are simulated: hexagonal, annular and quasi-random distributions. Using a quasi-random distribution significantly reduces the grating lobes. Furthermore, the simulations show the capability of the quasi-random array to electronically move the focal spot in the vicinity of the geometrical focus (up to +/- 15 mm). Based on the simulation study, the array is constructed and tested. The skull aberrations are corrected by using a time reversal mirror with amplitude correction achieved thanks to an implantable hydrophone, and a sharp focus is obtained through a human skull. Several lesions are induced in fresh liver and brain samples through human skulls, demonstrating the accuracy and the steering capabilities of the system.

Published
2003
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