1. Rapid and simplified synthesis of [ 18 F]Fluoromisonidazole and its use in PET imaging in an experimental model of subarachnoid hemorrhage.
- Author
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Fernandez-Maza L, Egea-Guerrero JJ, Balcerzyk M, Civantos-Jubera G, Gordillo-Escobar E, Vilches-Arenas A, Fernandez-Gomez I, Parrado-Gallego A, and Murillo-Cabezas F
- Subjects
- Animals, Chromatography, High Pressure Liquid, Disease Models, Animal, Humans, Male, Misonidazole chemical synthesis, Misonidazole chemistry, Misonidazole pharmacokinetics, Rats, Wistar, Solid Phase Extraction, Fluorine Radioisotopes chemistry, Misonidazole analogs & derivatives, Positron Emission Tomography Computed Tomography, Subarachnoid Hemorrhage diagnostic imaging
- Abstract
Cerebral damage secondary to the vasospasm due to subarachnoid hemorrhage (SAH) is an important cause of morbid-mortality. We propose the use of the PET tracer [
18 F]Fluoromisonidazole to visualize the hypoxia due to the vasospasm. On the other hand [18 F]Fluoromisonidazole synthesis process was optimized, avoiding HPLC purification using SPE cartridges instead, and reducing some synthesis steps. [18 F]Fluoromisonidazole in vitro stability was tested for ten hours, and in vivo PET/CT images showed higher cerebral uptake in hemorrhagic animals than in control rats., (Copyright © 2017 Elsevier Ltd. All rights reserved.)- Published
- 2018
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