1. Cryogenic Ion Spectroscopy of the Green Fluorescent Protein Chromophore in Vacuo.
- Author
-
Zagorec-Marks W, Foreman MM, Verlet JRR, and Weber JM
- Subjects
- Cold Temperature, Ions chemistry, Isomerism, Nitrogen chemistry, Photochemical Processes, Protein Conformation, Solvents chemistry, Spectrometry, Fluorescence methods, Thermodynamics, Vibration, Benzylidene Compounds chemistry, Fluorescent Dyes chemistry, Green Fluorescent Proteins chemistry, Imidazoles chemistry, Models, Molecular
- Abstract
We present the spectrum of the S
1 ← S0 transition of an anionic model for the chromophore of the green fluorescent protein in vacuo at cryogenic temperatures, showing previously unresolved vibrational features, and resolving the band origin at 20 930 cm-1 (477.8 nm) with unprecedented accuracy. The vibrational spectrum establishes that the molecule is in the Z isomer at low temperature. At increased temperature, the S1 ← S0 band shifts to the red, which we tentatively attribute to emergent population of the E isomer.- Published
- 2019
- Full Text
- View/download PDF