1. Lactose-containing hydrogels for enzyme stabilization.
- Author
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Huang, Yongshun, Chai, Qinyuan, Warmin, Mary R., and Ayres, Neil
- Subjects
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LACTOSE , *MONOMERS , *LACTASE , *POLYMER colloids , *COLLOIDAL gels , *HYDROGELS , *EXPANSION microscopy - Abstract
ABSTRACT A lactose-containing monomer, N-(2-lactosylethyl)acrylamide, was synthesized and polymerized with N-hydroxyethyl acrylamide and 1 wt % of N, N'-methylenebis(acrylamide) and potassium persulfate as the initiator to produce hydrogels. The weight percent of N-(2-lactosylethyl)acrylamide were increased from 0 to 100% in increments of 10%. Hydrogels were successfully produced with up to 90 wt % of N-(2-lactosylethyl)acrylamide. Gelation was confirmed by inverted vial tests and rheology measurements. The as-prepared hydrogels were used for papain stabilization against heat burden and papain that was loaded into hydrogels showed 45% more activity after heating as compared to papain that was heated without hydrogel stabilization. This hydrogel stabilization technique has potential applications in preserving enzyme activity. © 2016 Wiley Periodicals, Inc. J. Polym. Sci., Part A: Polym. Chem. 2016, 54, 2507-2514 [ABSTRACT FROM AUTHOR]
- Published
- 2016
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