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Lactose-containing hydrogels for enzyme stabilization.
- Source :
-
Journal of Polymer Science Part A: Polymer Chemistry . Aug2016, Vol. 54 Issue 16, p2507-2514. 9p. - Publication Year :
- 2016
-
Abstract
- ABSTRACT A lactose-containing monomer, N-(2-lactosylethyl)acrylamide, was synthesized and polymerized with N-hydroxyethyl acrylamide and 1 wt % of N, N'-methylenebis(acrylamide) and potassium persulfate as the initiator to produce hydrogels. The weight percent of N-(2-lactosylethyl)acrylamide were increased from 0 to 100% in increments of 10%. Hydrogels were successfully produced with up to 90 wt % of N-(2-lactosylethyl)acrylamide. Gelation was confirmed by inverted vial tests and rheology measurements. The as-prepared hydrogels were used for papain stabilization against heat burden and papain that was loaded into hydrogels showed 45% more activity after heating as compared to papain that was heated without hydrogel stabilization. This hydrogel stabilization technique has potential applications in preserving enzyme activity. © 2016 Wiley Periodicals, Inc. J. Polym. Sci., Part A: Polym. Chem. 2016, 54, 2507-2514 [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 0887624X
- Volume :
- 54
- Issue :
- 16
- Database :
- Academic Search Index
- Journal :
- Journal of Polymer Science Part A: Polymer Chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 116618390
- Full Text :
- https://doi.org/10.1002/pola.28127