1. Nests of dividing neuroblasts sustain interneuron production for the developing human brain.
- Author
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Paredes MF, Mora C, Flores-Ramirez Q, Cebrian-Silla A, Del Dosso A, Larimer P, Chen J, Kang G, Gonzalez Granero S, Garcia E, Chu J, Delgado R, Cotter JA, Tang V, Spatazza J, Obernier K, Ferrer Lozano J, Vento M, Scott J, Studholme C, Nowakowski TJ, Kriegstein AR, Oldham MC, Hasenstaub A, Garcia-Verdugo JM, Alvarez-Buylla A, and Huang EJ
- Subjects
- Animals, Animals, Newborn, Cell Movement, Cell Proliferation, Cerebral Cortex cytology, Cerebral Cortex embryology, Cerebral Cortex growth & development, GABAergic Neurons cytology, GABAergic Neurons physiology, Gene Expression Profiling, Gestational Age, Humans, Interneurons cytology, Median Eminence cytology, Median Eminence growth & development, Mice, Neural Stem Cells transplantation, Prosencephalon embryology, Prosencephalon growth & development, Transplantation, Heterologous, Interneurons physiology, Median Eminence embryology, Neural Stem Cells physiology, Neurogenesis, Prosencephalon cytology
- Abstract
The human cortex contains inhibitory interneurons derived from the medial ganglionic eminence (MGE), a germinal zone in the embryonic ventral forebrain. How this germinal zone generates sufficient interneurons for the human brain remains unclear. We found that the human MGE (hMGE) contains nests of proliferative neuroblasts with ultrastructural and transcriptomic features that distinguish them from other progenitors in the hMGE. When dissociated hMGE cells are transplanted into the neonatal mouse brain, they reform into nests containing proliferating neuroblasts that generate young neurons that migrate extensively into the mouse forebrain and mature into different subtypes of functional interneurons. Together, these results indicate that the nest organization and sustained proliferation of neuroblasts in the hMGE provide a mechanism for the extended production of interneurons for the human forebrain.
- Published
- 2022
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