1. Macrophages promote epithelial proliferation following infectious and non-infectious lung injury through a Trefoil factor 2-dependent mechanism.
- Author
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Hung LY, Sen D, Oniskey TK, Katzen J, Cohen NA, Vaughan AE, Nieves W, Urisman A, Beers MF, Krummel MF, and Herbert DR
- Subjects
- Animals, Bleomycin, CD11c Antigen metabolism, Cell Communication, Cell Proliferation, Cells, Cultured, Humans, Lung pathology, Lung Injury chemically induced, Lung Injury parasitology, Mice, Mice, Inbred C57BL, Mice, Knockout, Trefoil Factor-2 genetics, Wound Healing, Lung immunology, Lung Injury immunology, Macrophages physiology, Nippostrongylus immunology, Respiratory Mucosa physiology, Strongylida Infections immunology, Trefoil Factor-2 metabolism
- Abstract
Coordinated efforts between macrophages and epithelia are considered essential for wound healing, but the macrophage-derived molecules responsible for repair are poorly defined. This work demonstrates that lung macrophages rely upon Trefoil factor 2 to promote epithelial proliferation following damage caused by sterile wounding, Nippostrongylus brasiliensis or Bleomycin sulfate. Unexpectedly, the presence of T, B, or ILC populations was not essential for macrophage-driven repair. Instead, conditional deletion of TFF2 in myeloid-restricted CD11c
Cre TFF2flox mice exacerbated lung pathology and reduced the proliferative expansion of CD45- EpCAM+ pro-SPC+ alveolar type 2 cells. TFF2 deficient macrophages had reduced expression of the Wnt genes Wnt4 and Wnt16 and reconstitution of hookworm-infected CD11cCre TFF2flox mice with rWnt4 and rWnt16 restored the proliferative defect in lung epithelia post-injury. These data reveal a previously unrecognized mechanism wherein lung myeloid phagocytes utilize a TFF2/Wnt axis as a mechanism that drives epithelial proliferation following lung injury.- Published
- 2019
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