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In order to explore the effects of nitrapyrin (N-Serve) application on greenhouse gas emission and nitrogen (N) leaching of a waterlogged maize (Zea mays L.) field, we investigated the effects of applying nitrapyrin on soil ammonium (NH4+-N) and nitrate nitrogen (NO3−-N) content, nitrous oxide (N2O) fluxes, and the warming potential (GWPN2O) in a waterlogged maize field. The design included three treatments: waterlogging treatment with only urea application (V-3WL), waterlogging treatment with urea and nitrapyrin application (V-3WL+N), and no waterlogging treatment applying only urea (CK). Our results revealed that waterlogging led to the increase of nitrate concentrations across the soil profile, thus potentially increasing N leaching and decreasing N use efficiency. The accumulated N2O emissions increased significantly in waterlogged plots compared to control plots, and maximum N2O emission fluxes occurred during the process of soil drying after waterlogging; this resulted in an increase in GWPN2O and N2O greenhouse gas intensity (GHGIN2O) by 299% and 504%, respectively, compared to those of CK. However, nitrapyrin application was able to reduce N2O emissions. Nitrapyrin application was also good for decreasing GWPN2O and GHGIN2O by 34% and 50%, respectively, compared to V-3WL. In addition, nitrapyrin application was conducive to reduce N leaching and improve N use efficiency, resulting in a yield increase by 34%, compared to that of V-3WL. The application of nitrapyrin helped to mitigate agriculture-source greenhouse effects and N leaching induced by waterlogging, and was a high N-efficient fertilizer method for a waterlogged field. [ABSTRACT FROM AUTHOR]

Aims: To realize the so-called "Second Green Revolution", it is imperative to study the roots of crop plants, and identify those traits that improve the efficiency of nitrogen (N) acquisition. We aimed to evaluate how the N acquisition efficiency of six hybrid maize lines commonly grown in northern China depends on their root anatomy. Methods: Maize hybrids classified as having high nitrogen uptake efficiency (HNUE) and low nitrogen uptake efficiency (LNUE) were grown under high-N and low-N conditions in the greenhouse and the field. Results: Under N stress in the field and the greenhouse, HNUE increased shoot dry weight, root length density, N content and nitrogen use efficiency compared to LNUE. Low N availability increased the percentage of root cortical aerenchyma and the size of cortical cells. Root anatomy, with greater formation of root cortical aerenchyma and larger cortical cell size, was associated with increased specific nitrogen absorption efficiency (SNAE) and shoot biomass under N stress. Under low N availability, the percentage of aerenchyma and their total area had significant positive correlations with the shoot dry weight, total N uptake, SNAE. Conclusions: The results suggest that plants in limited N availability form more root cortical aerenchyma and have larger cortical cells, which is of benefit to root growth, soil exploration, N acquisition, and shoot biomass. These observations support the hypothesis that root anatomical phenotypes that affect the metabolic and construction costs of producing root length merit consideration as selection criteria for breeding to improve N acquisition in hybrid maize. [ABSTRACT FROM AUTHOR]

Humic acid plays an important role in improving grain yield and reducing N losses. In order to explore the effects of humic acid added to controlled-release fertilizer on summer maize yield, nitrogen use efficiency (NUE) and the characteristics of greenhouse gas (GHG) emissions in maize farmland soil, a two-year field experiment was set up. The treatments consisted of two fertilizers: 3% humic acid added to controlled-release fertilizer (HACRF), controlled-release fertilizer (CRF) and a control (without N fertilizer, N0). The results demonstrate that the yield and NUE of summer maize were significantly increased with the addition of humic acid in N fertilizer. Compared with N0 and CRF, the yield of maize was increased by 99.1% and 5.0%, respectively. Compared with CRF, the contents of soil ammonium–nitrogen (NH4+-N) and nitrate–nitrogen (NO3−-N) in HACRF were higher during early maize growth stage but lower during the late grain-filling stage. The NUE and soil nitrogen interdependent rate of HACRF were significantly increased by 4.6–5.4% and 7.2–11.6%, respectively, across the years compared with those of CRF. Moreover, the annual cumulative N2O emissions in HACRF were decreased by 29.0% compared with the CRF. Thus, the global warming potential and greenhouse emission intensity of HACRF were significantly decreased by 29.1% and 32.59%, respectively, compared with CRF. In conclusion, adding humic acid to controlled-release fertilizer can result in higher yield and nitrogen uptake, improve nitrogen use efficiency and reduce greenhouse gas emissions, which have better yield and environmental effects. [ABSTRACT FROM AUTHOR]

In order to clarify the effects of urea-ammonium nitrate solution (UAN) on the yield, nitrogen-use efficiency (NUE), and N2O emissions of summer maize under the condition of water and fertilizer integration, different types of nitrogen fertilizer were selected, namely, ordinary urea (urea) and UAN. Our results showed that the application of UAN was beneficial to improve the dry matter accumulation and the distribution of summer maize. Compared with urea treatment, the total nitrogen accumulation of UAN treatment was increased by 15.8%, and the harvest index was increased by 5.5%. The partial productivity, agronomic use efficiency, and recovery rate of nitrogen for UAN treatment were also increased by 9.1, 19.8, and 31.2%, respectively, compared to those of urea treatment. The soil nitrogen dependence rate treated with UAN was significantly decreased by 13.6%, compared to that of urea treatment. In addition, UAN was beneficial to reduce N2O emissions. The N2O warming potential (GWPN2O) and N2O greenhouse gas intensity (GHGIN2O) of urea treatment were 39.3 and 52.4% higher, compared to those of UAN treatment. The improvement of dry matter accumulation and distribution and nitrogen efficiency for UAN treatment were beneficial to increase the grain yield by 9.1%, compared to that of urea treatment. In conclusion, under the fertigation, the application of UAN favors higher yield and nitrogen uptake, with less soil nitrogen residue, higher NUE, and better environmental effect. [ABSTRACT FROM AUTHOR]

3 Root anatomy parameter of different NUE maize varieties at 40 days in the greenhouse under high-N and low-N conditions. A, RXSA, root cross-section area; B, TCA, total cortex cross-section area; C, TSA, total stele cross-section area; D, AA, total aerenchyma area; E, CCA, cortical cell area; F, MXVA, total metaxylem vessel area; G, CCS, median cortical cell size; H, MXA, median single metaxylem vessel area; I, AAP, percentage of aerenchyma area in the cortex; J, CFN, number of cortical cell files; K, MXN, number of metaxylem vessels; L, MXP, percentage of total metaxylem vessel area in the stele The gray bar and pink bar represent the HNUE and LNUE group, respectively. [Extracted from the article]

Leaf senescence is associated with fundamental changes on the level of the proteome and it can be modulated by nitrogen. To determine the precise regulatory mechanisms underlying these effects, we conducted a comparative proteomics study using 2-dimensional gel electrophoresis and MALDI-TOF/TOF MS. Based on our study of the maize leaf proteome, leaf senescence induces complex responses including the degradation of 32 senescence-associated proteins that are involved in many biological processes, especially energy, metabolism and cell rescue, defense and virulence pathways. Although similar conclusions have been highlighted in other crops, this study filled the knowledge gap in maize leaf senescence. Moreover, we discovered, for the first time, 29 “nitrogen-regulated senescence proteins” had significant ( P ≤ 0.05) interaction term for nitrogen × stage. Although further study of nitrogen-related senescence proteins, such as 30S ribosomal protein, will be required to fully elucidate their complex functions, the surprising results in our study provide a new vision to research the relationship between nitrogen and senescence. [ABSTRACT FROM AUTHOR]

Copyright of Transactions of the Chinese Society of Agricultural Engineering is the property of Chinese Society of Agricultural Engineering and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Transactions of the Chinese Society of Agricultural Engineering is the property of Chinese Society of Agricultural Engineering and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Soil nitrogen (N) shortage is a problem which affects many developing nations. Crops grown with low soil N levels show a marked decrease in the rate of photosynthesis and this deficiency reduces crop yield significantly. Therefore, developing a better understanding of the mechanisms by which low N levels cause decreased photosynthesis is crucial for maize agriculture. To better understand this process, we assessed the responses of photosynthesis traits and enzymatic activities in the summer maize cultivar Denghai 618 under field conditions with and without the use of N fertilisers. We measured photosynthesis parameters, and compared proteome compositions to identify the mechanisms of physiological and biochemical adaptations to N deficiency in maize. We observed that parameters that indicated the rate of photosynthesis decreased significantly under N deficiency, and this response was associated with leaf senescence. Moreover, we identified 37 proteins involved in leaf photosynthesis, and found that N deficiency significantly affected light-dependent and light-independent reactions in maize leaf photosynthesis. Although further analysis is required to fully elucidate the roles of these proteins in the response to N deficiency, our study identified candidate proteins which may be involved in the regulatory mechanisms involved in reduced photosynthesis under low N conditions in maize. [ABSTRACT FROM AUTHOR]

Irrigation, fertilization, and cultivation managements play important roles in crop production in the North China Plain (NCP). This study aims to compare crop yields, and water and N use efficiencies (WUE and NUE) in a wheat–maize cropping system under different integrated management practices and recommend the best management practices (BMPs). A two-year experiment involving four integrated management practices was conducted in Tai’an City, Shandong Province in the NCP. These management practices were designed as follows: (1) traditional farming practice (FP); (2) optimized combination of cropping and fertilization (OPT-1); (3) practice for high yield (HY), which does not consider the cost of resource inputs to maximize grain yield; and (4) further optimized combination of cropping and fertilization (OPT-2), which is based on the HY practice. Soil water movement, nitrate transport, and crop growth were all simulated using the soil water, heat, carbon, and N simulation (WHCNS) model. Results indicated that simulated soil water content and nitrate concentration at different depths in soil profiles, leaf area index, dry matter weight, and grain yield were all in good agreement with the field-measured data. Simulation results indicated that the amounts and dates of irrigation and fertilization, planting method, planting density, and sowing date had obvious effects on grain yield, water drainage, total N loss, WUE, and NUE. The annual average total N loss under the OPT-1 practice decreased by about 28.6% compared with the FP practice, whereas the annual average grain yield and NUE increased by 27.7% and 25.7%, respectively. The largest annual average grain yield and total N loss occurred in the HY practice (23,590 kg ha −1 and 240.6 kg N ha −1 , respectively). Although the annual average grain yield of the OPT-2 practice was 15.4% lower than that of HY practice, the NUE was 19.2% higher in OPT-2 than in HY. The annual average nitrate leaching under the OPT-2 practice was the lowest and reached 25.5%–60.0% compared with those under other practices. Among the four practices, the OPT-2 practice achieved the most preferable results; the lowest N loss and the highest NUE were obtained at the expense of a slight decrease in grain yield. Therefore, the OPT-2 practice was the BMPs among the four practices and should be recommended to maximize the economic and environmental benefits in the study region. [ABSTRACT FROM AUTHOR]

Optimizing the canopy nitrogen (N) distribution by matching the available light resources to construct a light-N matching canopy structure of summer maize is important to improve yield and narrow the yield gap (YG). A field experiment with four integrated agronomic management practice systems [a control treatment (T1), an improved management system (T2), a super high-yield management system (T3), and an optimized management system (T4)] was conducted at Da Wenkou Town, Tai'an, China, from June to October in -2018–2020 to study the canopy light and N distribution and photosynthetic N use efficiency of summer maize. The results showed that optimized integrated agronomic management practices could coordinate canopy light and N distributions to increase the yield, thus narrowing the YG between T1 and yield potential by 1.9–5.2 Mg ha-1. In addition, the T4 treatment intercepted more radiation by adjusting the leaf area. Furthermore, the leaf N content of T4 in the middle canopy was significantly increased. The coordination of canopy light and N distributions of T4 was increased significantly, which was conducive to maximizing the use of N and solar radiation and increasing photosynthetic N use efficiency significantly by 9.4–41.4%, compared to that of T1. As a result, the yield of T4 was increased by 37.0% compared to that of T1. And T4 narrowed the YG by 3.4 Mg ha-1. Therefore, the T4 was the best choice for achieving a high-yield and high-efficiency production of summer maize in this study. • Optimized agronomic management improved leaf nitrogen vertical distribution. • Optimized agronomic management increased canopy light interception. • Optimized agronomic management narrowed yield gap by regulating canopy structure. [ABSTRACT FROM AUTHOR]