1. Innovations in two-photon deep tissue microscopy
- Author
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Peter T. C. So, Ki Hean Kim, Christof Buehler, Chen-Yuan Dong, and Barry R. Masters
- Subjects
Fluorescence-lifetime imaging microscopy ,Microscopy, Confocal ,Microscopy, Video ,Materials science ,business.industry ,Confocal ,Biomedical Engineering ,General Medicine ,Fluorescence ,Fluorescence spectroscopy ,Cornea ,Optics ,Microscopy, Fluorescence ,Two-photon excitation microscopy ,Skin Physiological Phenomena ,Microscopy ,Medical imaging ,Animals ,Humans ,Photoactivated localization microscopy ,Rabbits ,business ,Skin - Abstract
The goal of this article is to provide a review of deep tissue studies based on two-photon microscopy. We will further explore three new developments that have significantly enhanced the power of two-photon imaging: (1) simultaneous two-photon fluorescence and confocal reflected-light imaging, (2) two-photon video-rate imaging, and (3) fluorescent spectroscopic measurements in deep tissue.
- Published
- 1999
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