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Your search keyword '"Scanferlato, Roberta"' showing total 2 results
Start Over Author "Scanferlato, Roberta" Topic palmitoleic acid
2 results on '"Scanferlato, Roberta"'

1. Fatty Acids in Membranes as Homeostatic, Metabolic and Nutritional Biomarkers: Recent Advancements in Analytics and Diagnostics.

Author

Ferreri, Carla, Masi, Annalisa, Sansone, Anna, Giacometti, Giorgia, Larocca, Anna Vita, Menounou, Georgia, Scanferlato, Roberta, Tortorella, Silvia, Rota, Domenico, Conti, Marco, Chatgilialoglu, Chryssostomos, Deplano, Simone, Louka, Maria, Maranini, Anna Rosaria, Salati, Arianna, and Sunda, Valentina

Subjects
FATTY acids, ERYTHROCYTES, BILAYER lipid membranes, CELL membranes, GAS chromatography
Abstract

Fatty acids, as structural components of membranes and inflammation/anti-inflammatory mediators, have well-known protective and regulatory effects. They are studied as biomarkers of pathological conditions, as well as saturated and unsaturated hydrophobic moieties in membrane phospholipids that contribute to homeostasis and physiological functions. Lifestyle, nutrition, metabolism and stress--with an excess of radical and oxidative processes--cause fatty acid changes that are examined in the human body using blood lipids. Fatty acid-based membrane lipidomics represents a powerful diagnostic tool for assessing the quantity and quality of fatty acid constituents and also for the follow-up of the membrane fatty acid remodeling that is associated with different physiological and pathological conditions. This review focuses on fatty acid biomarkers with two examples of recent lipidomic research and health applications: (i) monounsaturated fatty acids and the analytical challenge offered by hexadecenoic fatty acids (C16:1); and (ii) the cohort of 10 fatty acids in phospholipids of red blood cell membranes and its connections to metabolic and nutritional status in healthy and diseased subjects. [ABSTRACT FROM AUTHOR]

Published
2017
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2. Hexadecenoic fatty acid positional isomers and De Novo PUFA synthesis in colon cancer cells

Author

Anna Sansone, Marco De Spirito, Roberta Scanferlato, Carla Ferreri, Letizia Polito, Massimo Bortolotti, Andrea Bolognesi, Chryssostomos Chatgilialoglu, Giuseppe Maulucci, Scanferlato, Roberta, Bortolotti, Massimo, Sansone, Anna, Chatgilialoglu, Chryssostomo, Polito, Letizia, De Spirito, Marco, Maulucci, Giuseppe, Bolognesi, Andrea, and Ferreri, Carla

Subjects
Fluidity Functional two-photon microscopy Lipidomic analysis Membrane lipidomics Membrane remodelling Positional isomerismPUFA biosynthesis, Palmitic Acid, lcsh:Chemistry, Fatty Acids, Monounsaturated, Palmitic acid, chemistry.chemical_compound, 0302 clinical medicine, membrane lipidomic, Palmitoleic acid, lcsh:QH301-705.5, Phospholipids, Spectroscopy, chemistry.chemical_classification, 0303 health sciences, Chemistry, General Medicine, 3. Good health, Computer Science Applications, colon cancer, Linoleic Acids, Biochemistry, 030220 oncology & carcinogenesis, Colonic Neoplasms, lipids (amino acids, peptides, and proteins), Cholesterol Esters, lipidomic analysi, positional isomerism, Laurdan, PUFA biosynthesis, Polyunsaturated fatty acid, Sapienic acid, membrane remodelling, membrane lipidomics, lipidomic analysis, fluidity, functional two-photon microscopy, Palmitic Acids, PUFA biosynthesi, Settore FIS/07 - FISICA APPLICATA (A BENI CULTURALI, AMBIENTALI, BIOLOGIA E MEDICINA), Linoleoyl-CoA Desaturase, Article, Catalysis, Inorganic Chemistry, 03 medical and health sciences, Fatty Acids, Omega-3, Humans, biochemistry, Physical and Theoretical Chemistry, Molecular Biology, 030304 developmental biology, Cell Membrane, Organic Chemistry, Fatty acid, Metabolism, De novo synthesis, lcsh:Biology (General), lcsh:QD1-999, Microscopy, Fluorescence, Caco-2 Cells
Abstract

Palmitic acid metabolism involves delta-9 and delta-6 desaturase enzymes forming palmitoleic acid (9cis-16:1, n-7 series) and sapienic acid (6cis-16:1, n-10 series), respectively. The corresponding biological consequences and lipidomic research on these positional monounsaturated fatty acid (MUFA) isomers are under development. Furthermore, sapienic acid can bring to the de novo synthesis of the n-10 polyunsaturated fatty acid (PUFA) sebaleic acid (5cis,8cis-18:2), but such transformations in cancer cells are not known. The model of Caco-2 cell line was used to monitor sapienic acid supplementation (150 and 300 &mu, M) and provide evidence of the formation of n-10 fatty acids as well as their incorporation at levels of membrane phospholipids and triglycerides. Comparison with palmitoleic and palmitic acids evidenced that lipid remodelling was influenced by the type of fatty acid and positional isomer, with an increase of 8cis-18:1, n-10 PUFA and a decrease of saturated fats in case of sapienic acid. Cholesteryl esters were formed only in cases with sapienic acid. Sapienic acid was the less toxic among the tested fatty acids, showing the highest EC50s and inducing death only in 75% of cells at the highest concentration tested. Two-photon fluorescent microscopy with Laurdan as a fluorescent dye provided information on membrane fluidity, highlighting that sapienic acid increases the distribution of fluid regions, probably connected with the formation of 8cis-18:1 and the n-10 PUFA in cell lipidome. Our results bring evidence for MUFA positional isomers and de novo PUFA synthesis for developing lipidomic analysis and cancer research.

Published
2019
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