1. A Point Mutation in the Human Parainfluenza Virus Type 2 Nucleoprotein Leads to Two Separate Effects on Virus Replication
- Author
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Naoki Saka, Yusuke Matsumoto, Keisuke Ohta, Daniel Kolakofsky, and Machiko Nishio
- Subjects
viruses ,Immunology ,Nucleocapsid Proteins ,Virus Replication ,Microbiology ,Genome Replication and Regulation of Viral Gene Expression ,Cell Line ,Parainfluenza Virus 2, Human ,Virology ,Insect Science ,Animals ,Point Mutation ,Nucleocapsid ,Virus Release - Abstract
Paramyxovirus genomes, like that of human parainfluenza virus type 2 (hPIV2), have lengths of precisely multiples-of-six nucleotides (“rule of six”), where each nucleoprotein subunit (NP) binds exactly six nucleotides. Ten residues of its RNA binding groove contact the genome RNA; but only one, Q202, directly contacts a nucleotide base. The mutation of NP(Q202) leads to two phenotypes: the ability of the viral polymerase to replicate minigenomes with defective bipartite promoters where NP(wt) is inactive, and the inability to rescue rPIV2 carrying this point mutation by standard means. The absence of an rPIV2 NP(Q202A) prevented further study of the latter phenotype. By extensive and repeated cocultivation of transfected cells, an rPIV2 carrying this mutation was finally recovered, and this virus was apparently viable due to the presence of an additional NP mutation (I35L). Our results suggest that these two phenotypes are due to separate effects of the Q202 mutation, and that the problematic rescue phenotype may be due to the inability of the transfected cell to incorporate viral nucleocapsids during virus budding. IMPORTANCE Paramyxovirus genomes are contained within a noncovalent homopolymer of its nucleoprotein (NP) and form helical nucleocapsids (NC) whose 3′ ends contain the promoters for the initiation of viral RNA synthesis. This work suggests that these NC 3′ ends may play another role in the virus life cycle via their specific interaction with virus-modified cell membranes needed for the incorporation of viral NCs into budding virions.
- Published
- 2022