1. Endothelin-2/vasoactive intestinal contractor via ROCK regulates transglutaminase 1 on differentiation of mouse keratinocytes.
- Author
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Kotake-Nara E, Takizawa S, and Saida K
- Subjects
- Animals, Animals, Newborn, Cell Differentiation physiology, Intercellular Signaling Peptides and Proteins, Melanocytes cytology, Melanocytes metabolism, Mice, Signal Transduction physiology, rho-Associated Kinases, Endothelin-2 metabolism, Intracellular Signaling Peptides and Proteins metabolism, Keratinocytes cytology, Keratinocytes metabolism, Peptides metabolism, Protein Serine-Threonine Kinases metabolism, Transglutaminases metabolism
- Abstract
We previously found that endothelin-2/vasoactive intestinal contractor (ET-2/VIC) greatly increased in mouse epidermis after birth. In the present study, we evaluated whether ET-2/VIC expression was associated with the calcium-induced differentiation of cultured mouse keratinocytes. The differentiation induction was revealed by morphological change, cornified envelope (CE) formation, and involucrin and transglutaminase 1 (TG 1) expressions. ET-2/VIC gene expression and peptide production subsequently increased in the induction of the differentiation. We also found that Y-27632, a Rho-associated coiled-coil forming protein serine/threonine kinase (ROCK) inhibitor, suppressed up-regulation of ET-2/VIC gene expression, the induction of morphological change, the CE formation, and TG 1 expression, but not involucrin expression. These results indicate new three findings, (1) ET-2/VIC expression increases and has potential as a differentiation marker, (2) ET-2/VIC expression is mediated by ROCK, and (3) the ROCK regulated TG 1 expression, on the calcium-induced differentiation of mouse keratinocytes.
- Published
- 2007
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