1. Analysis of complex protein elution behavior in preparative ion exchange processes using a colloidal particle adsorption model.
- Author
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Briskot T, Hahn T, Huuk T, Wang G, Kluters S, Studts J, Wittkopp F, Winderl J, Schwan P, Hagemann I, Kaiser K, Trapp A, Stamm SM, Koehn J, Malmquist G, and Hubbuch J
- Subjects
- Adsorption, Antibodies, Monoclonal, Chromatography, Ion Exchange, Models, Chemical, Proteins chemistry, Proteins isolation & purification
- Abstract
A fundamental understanding of the protein retention mechanism in preparative ion exchange (IEX) chromatography columns is essential for a model-based process development approach. For the past three decades, the mechanistic description of protein retention has been based predominantly on the steric mass action (SMA) model. In recent years, however, retention profiles of proteins have been reported more frequently for preparative processes that are not consistent with the mechanistic understanding relying on the SMA model. In this work, complex elution behavior of proteins in preparative IEX processes is analyzed using a colloidal particle adsorption (CPA) model. The CPA model is found to be capable of reproducing elution profiles that cannot be described by the traditional SMA model. According to the CPA model, the reported complex behavior can be ascribed to a strong compression and concentration of the elution front in the lower unsaturated part of the chromatography column. As the unsaturated part of the column decreases with increasing protein load density, exceeding a critical load density can lead to the formation of a shoulder in the peak front. The general applicability of the model in describing preparative IEX processes is demonstrated using several industrial case studies including multiple monoclonal antibodies on different IEX adsorber systems. In this context, the work covers both salt controlled and pH-controlled protein elution., Competing Interests: Declaration of Competing Interest The authors affiliated to GoSilico declare that the software ChromX used in this study is a commercial product of GoSilico. The presented research does not depend on the usage of ChromX, the model can be implemented in any kind of modeling software., (Copyright © 2021. Published by Elsevier B.V.)
- Published
- 2021
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