1. Hybrid Small-Molecule/Protein Fluorescent Probes.
- Author
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Minoshima M, Reja SI, Hashimoto R, Iijima K, and Kikuchi K
- Subjects
- Humans, Animals, Small Molecule Libraries chemistry, Small Molecule Libraries metabolism, Fluorescent Dyes chemistry, Proteins chemistry, Proteins metabolism
- Abstract
Hybrid small-molecule/protein fluorescent probes are powerful tools for visualizing protein localization and function in living cells. These hybrid probes are constructed by diverse site-specific chemical protein labeling approaches through chemical reactions to exogenous peptide/small protein tags, enzymatic post-translational modifications, bioorthogonal reactions for genetically incorporated unnatural amino acids, and ligand-directed chemical reactions. The hybrid small-molecule/protein fluorescent probes are employed for imaging protein trafficking, conformational changes, and bioanalytes surrounding proteins. In addition, fluorescent hybrid probes facilitate visualization of protein dynamics at the single-molecule level and the defined structure with super-resolution imaging. In this review, we discuss development and the bioimaging applications of fluorescent probes based on small-molecule/protein hybrids.
- Published
- 2024
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