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Your search keyword '"mucin-degrading enzymes"' showing total 3 results
Start Over Descriptor "mucin-degrading enzymes" Topic proteomics
3 results on '"mucin-degrading enzymes"'

1. Gut Microbial Protein Expression in Response to Dietary Patterns in a Controlled Feeding Study: A Metaproteomic Approach

Author

Sheng Pan, Meredith A. J. Hullar, Lisa A. Lai, Hong Peng, Damon H. May, William S. Noble, Daniel Raftery, Sandi L. Navarro, Marian L. Neuhouser, Paul D. Lampe, Johanna W. Lampe, and Ru Chen

Subjects
microbiome, proteomics, dietary intervention, scfa, fatty acid metabolism, dysbiosis, mucin-degrading enzymes, Biology (General), QH301-705.5
Abstract

Although the gut microbiome has been associated with dietary patterns linked to health, microbial metabolism is not well characterized. This ancillary study was a proof of principle analysis for a novel application of metaproteomics to study microbial protein expression in a controlled dietary intervention. We measured the response of the microbiome to diet in a randomized crossover dietary intervention of a whole-grain, low glycemic load diet (WG) and a refined-grain, high glycemic load diet (RG). Total proteins in stools from 9 participants at the end of each diet period (n = 18) were analyzed by LC MS/MS and proteins were identified using the Human Microbiome Project (HMP) human gut microbiome database and UniProt human protein databases. T-tests, controlling for false discovery rate (FDR)

Published
2020
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2. Gut Microbial Protein Expression in Response to Dietary Patterns in a Controlled Feeding Study: A Metaproteomic Approach

Author

Marian L. Neuhouser, Daniel Raftery, Sandi L. Navarro, Ru Chen, William Stafford Noble, Meredith A. J. Hullar, Paul D. Lampe, Hong Peng, Johanna W. Lampe, Lisa A. Lai, Damon May, and Sheng Pan

Subjects
0301 basic medicine, Microbiology (medical), 030106 microbiology, Biology, Proteomics, Microbiology, Article, 03 medical and health sciences, chemistry.chemical_compound, proteomics, dietary intervention, Virology, Glycemic load, medicine, Food science, Microbiome, Shotgun proteomics, lcsh:QH301-705.5, Fatty acid metabolism, dysbiosis, medicine.disease, SCFA, 030104 developmental biology, lcsh:Biology (General), chemistry, fatty acid metabolism, mucin-degrading enzymes, Metaproteomics, Dysbiosis, Human Microbiome Project
Abstract

Although the gut microbiome has been associated with dietary patterns linked to health, microbial metabolism is not well characterized. This ancillary study was a proof of principle analysis for a novel application of metaproteomics to study microbial protein expression in a controlled dietary intervention. We measured the response of the microbiome to diet in a randomized crossover dietary intervention of a whole-grain, low glycemic load diet (WG) and a refined-grain, high glycemic load diet (RG). Total proteins in stools from 9 participants at the end of each diet period (n = 18) were analyzed by LC MS/MS and proteins were identified using the Human Microbiome Project (HMP) human gut microbiome database and UniProt human protein databases. T-tests, controlling for false discovery rate (FDR) &lt, 10%, were used to compare the Gene Ontology (GO) biological processes and bacterial enzymes between the two interventions. Using shotgun proteomics, more than 53,000 unique peptides were identified including microbial (89%) and human peptides (11%). Forty-eight bacterial enzymes were statistically different between the diets, including those implicated in SCFA production and degradation of fatty acids. Enzymes associated with degradation of human mucin were significantly enriched in the RG diet. These results illustrate that the metaproteomic approach is a valuable tool to study the microbial metabolism of diets that may influence host health.

Published
2020

3. Gut Microbial Protein Expression in Response to Dietary Patterns in a Controlled Feeding Study: A Metaproteomic Approach.

Author

Pan, Sheng, Hullar, Meredith A. J., Lai, Lisa A., Peng, Hong, May, Damon H., Noble, William S., Raftery, Daniel, Navarro, Sandi L., Neuhouser, Marian L., Lampe, Paul D., Lampe, Johanna W., and Chen, Ru

Subjects
PROTEIN expression, GUT microbiome, BACTERIAL enzymes, PROTEOMICS, MICROBIAL metabolism, FALSE discovery rate, HUMAN microbiota
Abstract

Although the gut microbiome has been associated with dietary patterns linked to health, microbial metabolism is not well characterized. This ancillary study was a proof of principle analysis for a novel application of metaproteomics to study microbial protein expression in a controlled dietary intervention. We measured the response of the microbiome to diet in a randomized crossover dietary intervention of a whole-grain, low glycemic load diet (WG) and a refined-grain, high glycemic load diet (RG). Total proteins in stools from 9 participants at the end of each diet period (n = 18) were analyzed by LC MS/MS and proteins were identified using the Human Microbiome Project (HMP) human gut microbiome database and UniProt human protein databases. T-tests, controlling for false discovery rate (FDR) <10%, were used to compare the Gene Ontology (GO) biological processes and bacterial enzymes between the two interventions. Using shotgun proteomics, more than 53,000 unique peptides were identified including microbial (89%) and human peptides (11%). Forty-eight bacterial enzymes were statistically different between the diets, including those implicated in SCFA production and degradation of fatty acids. Enzymes associated with degradation of human mucin were significantly enriched in the RG diet. These results illustrate that the metaproteomic approach is a valuable tool to study the microbial metabolism of diets that may influence host health. [ABSTRACT FROM AUTHOR]

Published
2020
Full Text
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