1. Role of the Pseudomonas aeruginosa las and rhl quorum-sensing systems in rhlI regulation.
- Author
-
de Kievit TR, Kakai Y, Register JK, Pesci EC, and Iglewski BH
- Subjects
- Bacterial Proteins genetics, Base Sequence, DNA-Binding Proteins genetics, Escherichia coli genetics, Escherichia coli metabolism, Ligases, Molecular Sequence Data, Operator Regions, Genetic, Pseudomonas aeruginosa genetics, Pseudomonas aeruginosa pathogenicity, Trans-Activators genetics, Transcription Factors genetics, Transcription, Genetic, Bacterial Proteins metabolism, DNA-Binding Proteins metabolism, Gene Expression Regulation, Bacterial, Pseudomonas aeruginosa growth & development, Trans-Activators metabolism, Transcription Factors metabolism
- Abstract
In Pseudomonas aeruginosa the LasR-LasI and RhlR-RhlI quorum-sensing (QS) systems control expression of numerous virulence genes in a population density-dependent fashion. In this study, we investigated regulation of the autoinducer synthase gene rhlI, which is responsible for C(4)-HSL signal production. Primer extension analysis was used to map the rhlI transcriptional start site and an upstream regulatory region was identified. Expression studies revealed that (i) this regulatory region is important for rhlI expression and (ii) although the rhl QS system will induce rhlI, las is the dominant regulator. Furthermore, we found that control of rhlI in Escherichia coli is markedly different than in P. aeruginosa.
- Published
- 2002
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