1. CCR4 Involvement in the Expansion of T Helper Type 17 Cells in a Mouse Model of Psoriasis.
- Author
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Matsuo K, Kitahata K, Kaibori Y, Arima Y, Iwama A, Ito M, Hara Y, Nagakubo D, Quan YS, Kamiyama F, Oiso N, Kawada A, Yoshie O, and Nakayama T
- Subjects
- Animals, Cell Communication drug effects, Cell Communication immunology, Cell Proliferation drug effects, Cells, Cultured, Coculture Techniques, Dendritic Cells immunology, Disease Models, Animal, Humans, Imiquimod administration & dosage, Imiquimod immunology, Mice, Mice, Transgenic, Primary Cell Culture, Psoriasis drug therapy, Psoriasis pathology, Receptors, CCR4 antagonists & inhibitors, Receptors, CCR4 genetics, Skin immunology, Th17 Cells drug effects, Th17 Cells metabolism, Psoriasis immunology, Receptors, CCR4 metabolism, Skin pathology, Th17 Cells immunology
- Abstract
Psoriasis is a chronic skin disease associated with T helper (Th)17-mediated inflammation. Because CCR4 is a major chemokine receptor expressed on Th17 cells, we investigated the role of CCR4 in a modified imiquimod-induced psoriasis model that showed enhanced skin infiltration of Th17 cells. CCR4-deficient mice had less severe skin disease than wild-type mice. Th17 cells were decreased in the skin lesions and regional lymph nodes of CCR4-deficient mice. In the regional lymph nodes of wild-type mice, CD44
+ memory Th17 cells expressing CCR4 were found to be clustered with dendritic cells expressing CCL22, a ligand for CCR4. Such dendritic cell‒Th17 cell clusters were significantly decreased in CCR4-deficient mice. Similar results were obtained using the IL-23‒induced psoriasis model. In vitro, compound 22, a CCR4 antagonist, significantly reduced the expansion of Th17 cells in the coculture of CD11c+ dendritic cells and CD4+ T cells separately prepared from the regional lymph nodes of wild-type mice with psoriasis. In vivo, compound 22 ameliorated the psoriasis-like skin disease in wild-type mice with significant decreases of Th17 cells in the regional lymph nodes and skin lesions. Collectively, CCR4 is likely to play a role in the pathogenesis of psoriasis through the expansion of Th17 cells., (Copyright © 2021 The Authors. Published by Elsevier Inc. All rights reserved.)- Published
- 2021
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