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In this study, a novel genotyping point-of-care testing (POCT) rapid detection device, the locked nucleic acid (LNA)-amplification refractory mutation system (ARMS)-recombinase polymerase amplification (RPA)-GoldMag lateral flow assay (LFA) platform, was provided by mining and synthesis based on prior technology. Research methods based on system-integrated innovation and knowledge-integrated generation have become a new trend in technology development. Here, we exploit the combination of LNA-coupled ARMS-RPA and gold nanoparticle probe technology for detection signal amplification, thus pioneering a new tool for accurate, rapid, and cost-effective genotyping. We also performed SNP typing detection and clinical validation of this new assay platform using common glucose-6-phosphate dehydrogenase (G6PD) gene single nucleotide polymorphism (SNP) loci, and the results demonstrated the high sensitivity, specificity, stability, accuracy and feasibility of the LNA-ARMS-RPA-GoldMag lateral flow assay platform. It is hoped that this new technology will make a significant contribution to the field of POCT rapid diagnosis and aim to expand the application space, reflecting its clinical application value and development prospects., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023. Published by Elsevier B.V.)

Foodborne illness caused by Salmonella spp. is one of the most prevalent public health problems globally, which have brought immeasurable economic burden and social impact to countries around the world. Neither current nucleic acid amplification detection method nor standard culture method (2–3 days) are suitable for field detection in areas with a heavy burden of Salmonella spp. Here, we developed a highly sensitive and accurate assay for Salmonella spp. detection in less than 40 min. Specifically, the invA gene of Salmonella spp. was amplified by recombinase polymerase amplification (RPA), followed by Pyrococcus furiosus Argonaute (Pf Ago)-based target sequence cleavage, which could be observed by a fluorescence reader or the naked eye. The assay offered the lowest detectable concentration of 1.05 × 101 colony forming units/mL (CFU/mL). This assay had strong specificity and high sensitivity for the detection of Salmonella spp. in field samples, which indicated the feasibility of this assay. • The method can be widely used in on-site food sample detection or standard laboratory detection. • The operation process is simple and fast (∼40 min). • High detection sensitivity • Ultraviolet light can read signals. [ABSTRACT FROM AUTHOR]

At present, the main challenge for Staphylococcus aureus (S. aureus) in food safety is still a lack of rapid, convenient detection methods. This study developed a combination of recombinase polymerase amplification (RPA) and the CRISPR-Cas12a technology (known as the RPA-CRISPR-Cas12a assay) for the detection of S. aureus in food. This novel assay could detect samples with a fluorescence reader, lateral flow dipstick (LFD) or the naked eye under a UV transilluminator. After optimization, the RPA-CRISPR-Cas12a assay could specifically and stably detect S. aureus as low as 1–10 copies/μL within 40 min at 37 °C. This assay had high sensitivity to the detection of S. aureus in both laboratory standard samples and field samples, which indicated the feasibility of this assay. To sum up, this accurate, portable detection method for S. aureus has great potential applications in food safety, especially in the field, without requiring sophisticated equipment or high level of technical expertise. [ABSTRACT FROM AUTHOR]

• A visual method based on RPA and LFD for identification of meat adulteration (beef, sheep, pork, duck, chicken) was firstly developed. • The identification reaction was completed within 20 min. • The identification results could be determined by the naked eye. • The detection limits of the RPA-LFD assays for duck, beef, sheep, chicken, and pork were 101/µL, 102/µL, 102/µL, 101/µL and 101/µL, respectively. • The RPA-LFD assays could differentiate species in boiled, microwaved, pressure -cooked or fried samples. Determining the animal source in meat and meat products is crucial to prevent meat adulteration and fraud. Conventional methods require considerable operator skills, expensive instruments and are unable to provide fast mobile on-site detection systems to detect contamination of meat products. We developed a visual method based on recombinase polymerase amplification (RPA) and a lateral flow dipstick (LFD) to identify beef (Bos taurus), sheep (Ovis aries), pork (Sus scrofa), duck (Anas platyrhynchos) and chicken (Gallus gallus). The reaction was completed within 20 min. The results were determined by the naked eye. The detection limits of the RPA-LFD assays for duck, beef, sheep, chicken and pork were 101/µL, 102/µL, 102/µL, 101/µL and 101/µL, respectively. Furthermore, the RPA-LFD assays could differentiate species in boiled, microwaved, pressure-cooked or fried samples. These RPA-LFD assays represent a rapid, mobile detection system for determining meat product contamination. [ABSTRACT FROM AUTHOR]