1. Th17 expression and IL-17 levels in laryngeal squamous cell carcinoma patients.
- Author
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Li, Fu-Jun, Cai, Zhi-Jian, Yang, Fei, Zhang, Shou-De, and Chen, Ming
- Subjects
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RNA analysis , *ACADEMIC medical centers , *CHI-squared test , *CONFIDENCE intervals , *ENZYME-linked immunosorbent assay , *FISHER exact test , *FLOW cytometry , *IMMUNOHISTOCHEMISTRY , *INTERLEUKINS , *RESEARCH methodology , *POLYMERASE chain reaction , *PROBABILITY theory , *RESEARCH funding , *SQUAMOUS cell carcinoma , *STATISTICS , *T cells , *T-test (Statistics) , *TISSUE culture , *TUMOR classification , *DATA analysis , *REVERSE transcriptase polymerase chain reaction , *DATA analysis software , *DESCRIPTIVE statistics , *MANN Whitney U Test ,LARYNGEAL tumors - Abstract
ConclusionThe Th17 cell frequency in peripheral blood and levels of IL-17 showed significant differences between patients with laryngeal squamous cell carcinoma and those with vocal cords polyps. Serum levels of IL-17 were correlated with laryngocarcinoma staging.ObjectivesTo investigate associations among the frequency of Th17 cells, levels of IL-17, and laryngeal squamous cell carcinoma.MethodEighty in-patients with laryngeal squamous cell carcinoma and 114 in-patients with polypus of the vocal cord were enrolled. Th17 cell frequencies in peripheral blood and serum levels of IL-17 were measured by flow cytometry and enzyme-linked immunosorbent assay, respectively. The tissue expression levels of IL-17 mRNA transcripts and protein were measured using quantitative RT-PCR or immunohistochemical detection, respectively.ResultsTh17 cell frequencies in peripheral blood and serum concentrations of IL-17 were significantly higher in patients with laryngocarcinoma compared with those in patients with polyps (p < 0.01 for both Th17 cells and IL-17 levels). Serum concentrations of IL-17 were significantly higher in patients with advanced laryngocarcinoma than in patients with early laryngocarcinoma (p < 0.01). The mRNA and protein levels of IL-17 were significantly higher in laryngocarcinoma tissues than in adjacent normal tissues (p < 0.01 for mRNA levels,p < 0.05 for protein levels). [ABSTRACT FROM PUBLISHER]
- Published
- 2016
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