1. P-Body Purification Reveals the Condensation of Repressed mRNA Regulons.
- Author
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Hubstenberger A, Courel M, Bénard M, Souquere S, Ernoult-Lange M, Chouaib R, Yi Z, Morlot JB, Munier A, Fradet M, Daunesse M, Bertrand E, Pierron G, Mozziconacci J, Kress M, and Weil D
- Subjects
- Cell Fractionation, Cytoplasm metabolism, Cytoplasmic Granules chemistry, Cytoplasmic Granules metabolism, Gene Ontology, HEK293 Cells, HeLa Cells, Humans, Molecular Sequence Annotation, Phase Transition, Protein Biosynthesis, Proteome metabolism, RNA Stability, RNA, Messenger metabolism, Ribonucleoproteins metabolism, Gene Expression Regulation, Proteome genetics, RNA, Messenger genetics, Regulon, Ribonucleoproteins genetics
- Abstract
Within cells, soluble RNPs can switch states to coassemble and condense into liquid or solid bodies. Although these phase transitions have been reconstituted in vitro, for endogenous bodies the diversity of the components, the specificity of the interaction networks, and the function of the coassemblies remain to be characterized. Here, by developing a fluorescence-activated particle sorting (FAPS) method to purify cytosolic processing bodies (P-bodies) from human epithelial cells, we identified hundreds of proteins and thousands of mRNAs that structure a dense network of interactions, separating P-body from non-P-body RNPs. mRNAs segregating into P-bodies are translationally repressed, but not decayed, and this repression explains part of the poor genome-wide correlation between RNA and protein abundance. P-bodies condense thousands of mRNAs that strikingly encode regulatory processes. Thus, we uncovered how P-bodies, by condensing and segregating repressed mRNAs, provide a physical substrate for the coordinated regulation of posttranscriptional mRNA regulons., (Copyright © 2017 Elsevier Inc. All rights reserved.)
- Published
- 2017
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