1. Real time imaging of cell-permeable nanoreactor with SERS for insight into cellular processes.
- Author
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Zhang SY, Zhou XY, Chen HY, Deng LY, Li DW, Lv J, and Qian RC
- Subjects
- Humans, HeLa Cells, Hydrogen Peroxide analysis, Hydrogen Peroxide chemistry, Glucose Oxidase chemistry, Glucose Oxidase metabolism, Spectrum Analysis, Raman methods, Gold chemistry, Metal Nanoparticles chemistry, Glucose analysis, Glucose metabolism
- Abstract
Intracellular glucose detection is crucial due to its pivotal role in metabolism and various physiological processes. Precise glucose monitoring holds significance in diabetes management, metabolic studies, and biotechnological applications. In this study, we developed an innovative and expedient cell-permeable nanoreactor for intracellular glucose based on surface-enhanced Raman scattering (SERS). The nanoreactor was designed with gold nanoparticles (AuNPs), which were engineered with glucose oxide (GOx) and a H
2 O2 -responsive Raman reporter 2-mercaptohydroquinone (2-MHQ). The interaction between 2-MHQ and H2 O2 generated by glucose and GOx could simultaneously induce the appearance in the peak at 985 cm-1 . Our results showed excellent performance in detecting glucose within the concentration range from 0.1 μM to 10 mM, with a low detection limitation of 14.72 nM. In addition, the glucose distribution in single HeLa cells was evaluated by real time SERS mapping. By combining noble metal particles and natural oxidases, the nanoreactor possesses both Raman activity and enzymatic functionality, thus enables sensitive glucose detection and facilitates imaging at a single cell level, which offers an insightful monitoring of cellular processes., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)- Published
- 2024
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