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Superheated steam treatment (SST) can improve cereal-based foodstuff quality by altering the gluten properties. In the current study we investigate the effect of SST on the polymerization behavior, conformational rearrangement and microscopic characteristics of gluten, which contribute to determining the molecular mechanisms of SST that impact the gluten properties. Compared with native wheat gluten, SST was observed to induce an increase in sodium dodecyl sulfate (SDS) insoluble gluten. This indicates that SST promoted the polymerization behavior of gluten via the cross-linking of disulfide (SS) bonds. In addition, hydrogen bonds were observed to develop from the micro-environment variation of tryptophan (Trp) residue in the gluten. This consequently had a synergistic effect on the secondary structure, increasing the proportion of intermolecular β-sheets and α-helices. Furthermore, SST enhanced the molecular surface roughness and weakened the gluten network structure. This study could contribute to insight into the improvement of flour quality following SST. • Conformational rearrangement and microscopic properties of gluten were studied. • Superheated steam treatment buried the tryptophan residue and tyrosine doublet. • Superheated steam treatment rearranged disulfide conformation. • Superheated steam treatment roughened the surface micro-structure of gluten. [ABSTRACT FROM AUTHOR]

Superheated steam treatment (SST) can improve cereal-based foodstuff quality. Starch is the major component of cereal-based foodstuffs, playing a key role in food quality. For understanding the effect of SST on the moisture distribution, lamellar structure and molecular weight of wheat starch, the low-field nuclear magnetic resonance (LF-NMR), small angle X-ray scattering (SAXS) and size exclusion chromatography were employed. Compared with native starch, SST reduced the average thickness of crystalline layer and induced the depolymerization of starch molecules, which were attributed to the limited moisture transfer resulting in the partial gelatinization of starch granules. The structural variations had a synergistic effect on the rheological properties of starch samples. Upon SST, treated samples exhibited a weaker pseudoplasticity behavior and increased the deformability and mobility of gels systems. Furthermore, the lower tanδ values of treated starch samples were observed, indicating that the gels systems tended to the solid-like behaviors. [Display omitted] • Superheated steam treatment (SST) changed the water distribution of wheat starch. • SST transmuted the crystalline lamellae into the amorphous lamellae of wheat starch. • SST depolymerized starch molecular structure and decreased its molecular weight. • SST weakened the gels systems but increased its deformability and mobility. [ABSTRACT FROM AUTHOR]

This paper explored the impact of superheated steam (SS) treatment on the physicochemical, crystalline characteristics and in vitro digestibility of wheat starch. Upon SS treatment, treated samples showed lower swelling power, solubility, and viscosities, but had higher gelatinization temperature, which might be due to the partial gelatinization of starch granules. This agreed with micromorphology characterization, indicating the degradation of Maltese cross. In addition, SS treatment decreased the long-range crystalline and short-range order of starch, compared to native starch sample. This change of physicochemical and structural properties was responsible for the decrease of starch hydrolysis rate. Meanwhile, SS treatment obviously increased the slowly digestible starch (SDS) and resistant starch (RS) content from 10.6% to 7.9% (native starch) to 17.2% and 13.6% (150 °C, 1 min), respectively. This knowledge indicates that superheat steam technology possibly is a promising strategy in starch modification. [Display omitted] • Superheated steam (SS) treatment induced partial gelatinization of wheat starch. • SS treatment destroyed the long-range crystalline structure of wheat starch. • SS treatment increased resistant starch contents of wheat starch. [ABSTRACT FROM AUTHOR]

Wheat flour superheated steam treatment can improve the baking food quality by modifying the functionality of wheat flour. To reveal the underlying mechanism of improving wheat flour quality by superheated steam treatment, the gluten molecular rearrangement and polymerization behavior of wheat flour upon superheated steam were studied. Molecular size distributions of samples were analyzed using size exclusion (SE-HPLC) and electrophoresis, which illustrated that superheated steam treatment promoted the aggregation of gluten due to cross-links of disulfide (SS) bonds. Superheated steam treatment increased the SDS-insoluble proteins content from 63.07% (native wheat flour) to 93.32% (treated at 150 °C for 1 min). Infrared spectroscopy (FTIR) was used to calculate the different secondary structures percentages of gluten, suggesting that superheated steam treatment increased intermolecular β-sheet and α-helix while it decreased anti-parallel β-sheet, β-turn and β-sheet. Furthermore, thermo gravimetric analysis (TGA) and rheology suggested that superheated steam treatment enhanced gluten mass but weakened gel system due to molecular rearrangements of SS bonds and hydrogen bonds. [Display omitted] • Gluten polymerization behavior of wheat flour upon superheated steam were studied. • Superheated steam treatment induced gluten aggregation and SS bonds cross-links. • Superheated steam treatment weakened the wheat flour gel system. [ABSTRACT FROM AUTHOR]

The effect of superheated steam treatment on the physicochemical properties of wheat flour and quality characteristics of cake were investigated. Superheated steam treatment significantly increased the swelling power, viscosities and gelatinization temperature, but reduced the gelatinization enthalpy (p < 0.05), compared with native wheat flour. However, the dough made from the treated flour was harder to form. Moreover, superheated steam treatment significantly increased the value of falling number. Cake quality was affected by the changes in the physicochemical properties of wheat flour. Results showed that superheated steam treatment significantly decreased the hardness of cake from 1465 g (native flour) to 377 g (150 °C, 1 min), and increased the specific volume of cake from 3.12 mL/g (native flour) to 3.9 mL/g (150 °C, 1 min). The present study suggests that superheated steam treatment has great potential as a flour modification technology for cake quality improvement. • Physicochemical properties of wheat flour treated with superheated steam were studied. • Superheated steam treatment weakened the gluten strength of dough. • Superheated steam treatment promoted the functional properties of wheat flour. • Wheat flour superheated steam treatment could improve cake quality characteristics. [ABSTRACT FROM AUTHOR]

The structural and digestive properties of wheat flour which had been treated with superheated steam were investigated. Superheated steam treatment increased the degree of gelatinization (DG) and water absorption indexes (WAI) while decreased water solubility indexes (WSI) of wheat flour samples, compared with native wheat flour. The structural properties of wheat flour were characterized using scanning electron microscopy (SEM), confocal laser scanning microscopy (CLSM), particle size analyzer, X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR). The starch granules agglomeration and denatured gluten proteins aggregation of samples were observed after treated with superheated steam. Additionally, Superheated steam treatment decreased the relative crystallinity and short-range order structure of starch. These structural changes were related to the starch digestibility. Superheated steam treatment caused the significant increase for slowly digestible starch (SDS) contents from 21.5% of native wheat flour to 35.0% of treated wheat flour (170 °C, 4 min). Additionally, the equilibrium hydrolysis concentration (C ∞) and the kinetic coefficient (k) of treated wheat flour were lower than native wheat flour, indicating that superheated steam treatment decelerated the hydrolysis rate. These results contributed to the applications of superheated steam technology in wheat flour modification. Image 1 • Structure and digestibility properties of wheat flour were studied. • Gluten aggregation of wheat flour was observed by superheated steam treatment. • Superheated steam treatment changed the structural characteristics of wheat flour. • Superheated steam treatment increased SDS and RS contents of wheat flour. [ABSTRACT FROM AUTHOR]