1. Reduction of ROS-HIF1α-driven glycolysis by taurine alleviates Streptococcus uberis infection.
- Author
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Lan R, Zhou Y, Wang Z, Fu S, Gao Y, Gao X, Zhang J, Han X, Phouthapane V, Xu Y, and Miao J
- Subjects
- Animals, Cell Line, Female, Mammary Glands, Animal cytology, Mice, Mice, Inbred C57BL, Signal Transduction drug effects, Streptococcus drug effects, Glycolysis drug effects, Hypoxia-Inducible Factor 1, alpha Subunit metabolism, Reactive Oxygen Species metabolism, Streptococcal Infections metabolism, Taurine pharmacology
- Abstract
Antibiotic-resistant strains of Streptococcus uberis ( S. uberis ) frequently cause clinical mastitis in dairy cows resulting in enormous economic losses. The regulation of immunometabolism is a promising strategy for controlling this bacterial infection. To investigate whether taurine alleviates S. uberis infection by the regulation of host glycolysis via HIF1α, the murine mammary epithelial cell line (EpH4-Ev) and C57BL/6J mice were challenged with S. uberis. Our data indicate that HIF1α-driven glycolysis promotes inflammation and damage in response to the S. uberis challenge. The activation of HIF1α is dependent on mTOR-mediated ROS production. These results were confirmed in vivo . Taurine, an intracellular metabolite present in most animal tissues, has been shown to effectively modulate HIF1α-triggered metabolic reprogramming and contributes to a reduction of inflammation, which reduces mammary tissue damage and prevents mammary gland dysfunction in S. uberis -induced mastitis. These data provide a novel putative prophylactic and therapeutic strategy for amelioration of dairy cow mastitis and bacterial inflammation.
- Published
- 2022
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