1. Immunomodulating pectins from root bark, stem bark, and leaves of the Malian medicinal tree Terminalia macroptera, structure activity relations.
- Author
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Zou YF, Barsett H, Ho GT, Inngjerdingen KT, Diallo D, Michaelsen TE, and Paulsen BS
- Subjects
- Carbohydrate Sequence, Complement System Proteins metabolism, Humans, Hydrolysis, Immunologic Factors isolation & purification, Immunologic Factors metabolism, Molecular Sequence Data, Monosaccharides analysis, Pectins isolation & purification, Pectins metabolism, Plant Bark chemistry, Plant Leaves chemistry, Plant Roots chemistry, Polygalacturonase metabolism, Structure-Activity Relationship, Immunologic Factors chemistry, Immunologic Factors pharmacology, Pectins chemistry, Pectins pharmacology, Terminalia chemistry, Trees chemistry
- Abstract
The root bark, stem bark, and leaves of Terminalia macroptera were sequentially extracted with ethanol, 50% ethanol-water, and 50°C water using an accelerated solvent extractor (ASE). Six bioactive purified pectic polysaccharide fractions were obtained from the 50°C crude water extracts after anion exchange chromatography and gel filtration. The root bark, stem bark, and leaves of T. macroptera were all good sources for fractions containing bioactive polysaccharides. The high molecular weight fraction 50WTRBH-I-I, being the most active fraction in the complement fixation test, has a highly ramified rhamnogalacturonan type I (RG-I) region with arabinogalactan type II (AG-II) side chains. The most abundant fractions from each plant part, 50WTRBH-II-I, 50WTSBH-II-I, and 50WTLH-II-I, were chosen for pectinase degradation. The degradation with pectinase revealed that the main features of these fractions are that of pectic polysaccharides, with hairy regions (RG-I regions) and homogalacturonan regions. The activity of the fractions obtained after pectinase degradation and separation by gel filtration showed that the highest molecular weight fractions, 50WTRBH-II-Ia, 50WTSBH-II-Ia, and 50WTLH-II-Ia, had higher complement fixation activity than their respective native fractions. These results suggest that the complement fixation activities of these pectins are expressed mainly by their ramified regions., (Copyright © 2014 Elsevier Ltd. All rights reserved.)
- Published
- 2015
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