1. Recombinant human bone morphogenetic protein 2 (rhBMP-2) immobilized on laser-fabricated 3D scaffolds enhance osteogenesis.
- Author
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Chatzinikolaidou M, Pontikoglou C, Terzaki K, Kaliva M, Kalyva A, Papadaki E, Vamvakaki M, and Farsari M
- Subjects
- Adsorption, Alkaline Phosphatase genetics, Alkaline Phosphatase metabolism, Bone Marrow Cells cytology, Bone Marrow Cells metabolism, Bone Morphogenetic Protein 2 genetics, Bone Morphogenetic Protein 2 metabolism, Cell Differentiation drug effects, Cell Proliferation drug effects, Collagen genetics, Collagen metabolism, Core Binding Factor Alpha 1 Subunit genetics, Core Binding Factor Alpha 1 Subunit metabolism, Extracellular Matrix drug effects, Extracellular Matrix metabolism, Gene Expression Regulation, Humans, Immobilized Proteins genetics, Immobilized Proteins metabolism, Immobilized Proteins pharmacology, Lasers, Mesenchymal Stem Cells cytology, Mesenchymal Stem Cells metabolism, Osteocalcin genetics, Osteocalcin metabolism, Osteogenesis genetics, Osteonectin genetics, Osteonectin metabolism, Primary Cell Culture, Recombinant Proteins genetics, Recombinant Proteins metabolism, Recombinant Proteins pharmacology, Tissue Engineering, Bone Marrow Cells drug effects, Bone Morphogenetic Protein 2 pharmacology, Mesenchymal Stem Cells drug effects, Osteogenesis drug effects, Tissue Scaffolds
- Abstract
The regeneration of bone via a tissue engineering approach involves components from the macroscopic to the nanoscopic level, including appropriate 3D scaffolds, cells and growth factors. In this study, hexagonal scaffolds of different diagonals were fabricated by Direct Laser Writing using a photopolymerizable hybrid material. The proliferation of bone marrow (BM) mesenchymal stem cells (MSCs) cultured on structures with various diagonals, 50, 100, 150 and 200μm increased significantly after 10days in culture, however without significant differences among them. Next, recombinant human bone morphogenetic protein 2 (rhBMP-2) was immobilized onto the hybrid material both via covalent binding and physical adsorption. Both immobilization types exhibited similar high releaseate bioactivity profiles and a sustained delivery of rhBMP-2. The collagen and calcium levels produced in the extracellular matrix (ECM) were significantly elevated for the samples functionalized with BMP-2 compared to those in the osteogenic medium. Furthermore, significant upregulation of gene expression in both types of BMP-2 immobilized scaffolds was observed for alkaline phosphatase (ALPL) and osteocalcin (BGLAP) at days 7, 14, and 21, for RUNX2 at day 21, and for osteonectin (SPARC) at days 7 and 14. The results suggest that the release of bioactive rhBMP-2 from the hybrid scaffolds enhance the control over the osteogenic differentiation during cell culture., (Copyright © 2016 Elsevier B.V. All rights reserved.)
- Published
- 2017
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