1. Crystallization and preliminary X-ray diffraction analysis of the cytosolic domain of the Mg2+ transporter MgtE.
- Author
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Tanaka, Yoshiki, Hattori, Motoyuki, Fukai, Shuya, Ishitania, Ryuichiro, and Nureki, Osamu
- Subjects
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CRYSTALLIZATION , *X-ray diffraction , *CYTOSOL , *MAGNESIUM ions , *CYSTATHIONINE gamma-lyase , *CARRIER proteins , *SYNCHROTRON radiation - Abstract
The MgtE family of Mg2+ transporters is ubiquitously conserved in all domains of life. The cytosolic domains of the MgtE Mg2+ transporters include a cystathionine-β-synthase (CBS) domain which is known to play a regulatory function in transporter proteins. The cytosolic domain of MgtE from Thermus thermophilus was overexpressed, purified and crystallized in the presence and absence of Mg2+. The crystals formed in the presence of Mg2+ diffracted X-rays to 2.3 Å resolution using synchrotron radiation, belong to space group P6522 with unit-cell parameters a = b = 57.7, c = 317.6 Å and are expected to contain one molecule in the asymmetric unit. The crystals formed in the absence of Mg2+ diffracted X-rays to 3.5 Å resolution using synchrotron radiation, belong to space group P212121 with unit-cell parameters a = 77.0, b = 100.3, c = 100.3 Å and are expected to contain two molecules in the asymmetric unit. [ABSTRACT FROM AUTHOR]
- Published
- 2007
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