1. MALDI-TOF MS identification of Anopheles gambiae Giles blood meal crushed on Whatman filter papers
- Author
-
Niare, Sirama, Almeras, Lionel, Tandina, Fatalmoudou, Yssouf, Amina, Bacar, Affane, Toilibou, Ali, Doumbo, Ogobara, Raoult, Didier, Parola, Philippe, Unité de Recherche sur les Maladies Infectieuses Tropicales Emergentes (URMITE), Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes (URMITE), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR48, Institut des sciences biologiques (INSB-CNRS)-Institut des sciences biologiques (INSB-CNRS)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR48, Institut des sciences biologiques (INSB-CNRS)-Institut des sciences biologiques (INSB-CNRS)-Centre National de la Recherche Scientifique (CNRS), Département d’Infectiologie de Terrain, Unité de Parasitologie, Institut de Recherche Biomédicale des Armées [Brétigny-sur-Orge] (IRBA), Programme Nationale de Lutte Contre le Paludisme, Ministry of Public Health, Malaria Research and Training Centre, Université de Bamako-Faculty of Medicine, Pharmacy, Sciences Economiques et Sociales de la Santé & Traitement de l'Information Médicale (SESSTIM - U912 INSERM - Aix Marseille Univ - IRD), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM), ANR-11-IDEX-0001,Amidex,INITIATIVE D'EXCELLENCE AIX MARSEILLE UNIVERSITE(2011), INSB-INSB-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR48, INSB-INSB-Centre National de la Recherche Scientifique (CNRS), Institut de Recherche Biomédicale des Armées (IRBA), Institut de Recherche pour le Développement (IRD)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Aix Marseille Université (AMU), COMBE, Isabelle, and INITIATIVE D'EXCELLENCE AIX MARSEILLE UNIVERSITE - - Amidex2011 - ANR-11-IDEX-0001 - IDEX - VALID
- Subjects
Physiology ,lcsh:Medicine ,Disease Vectors ,Mosquitoes ,Mass Spectrometry ,Analytical Chemistry ,Spectrum Analysis Techniques ,[SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases ,Abdomen ,Medicine and Health Sciences ,lcsh:Science ,Body Fluids ,Matrix-assisted laser desorption ionization time-of-flight mass spectrometry ,Insects ,Laboratory Equipment ,Chemistry ,Infectious Diseases ,Blood ,Physical Sciences ,Vertebrates ,[SDV.MHEP.MI] Life Sciences [q-bio]/Human health and pathology/Infectious diseases ,Engineering and Technology ,[SDV.IB]Life Sciences [q-bio]/Bioengineering ,Anatomy ,Research Article ,Paper ,Arthropoda ,Equipment ,Research and Analysis Methods ,Filter paper ,Anopheles ,parasitic diseases ,Parasitic Diseases ,Animals ,[SDV.IB] Life Sciences [q-bio]/Bioengineering ,lcsh:R ,Organisms ,Biology and Life Sciences ,Insect Bites and Stings ,Feeding Behavior ,Tropical Diseases ,Anopheles gambiae ,Invertebrates ,Insect Vectors ,Malaria ,[SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology ,Species Interactions ,Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ,[SDV.BA.ZI] Life Sciences [q-bio]/Animal biology/Invertebrate Zoology ,lcsh:Q - Abstract
International audience; Background: Identification of the source of mosquito blood meals is an important component for disease control and surveillance. Recently, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) profiling has emerged as an effective tool for mosquito blood meal identification, using the abdomens of freshly engorged mosquitoes. In the field, mosquito abdomens are crushed on Whatman filter papers to determine the host feeding patterns by identifying the origin of their blood meals. The aim of this study was to test whether crushing engorged mosquito abdomens on Whatman filter papers was compatible with MALDI-TOF MS for mosquito blood meal identification. Both laboratory reared and field collected mosquitoes were tested.Material and methods: Sixty Anopheles gambiae Giles were experimentally engorged on the blood of six distinct vertebrate hosts (human, sheep, rabbit, dog, chicken and rat). The engorged mosquito abdomens were crushed on Whatman filter papers for MALDI-TOF MS analysis. 150 Whatman filter papers, with mosquitoes engorged on cow and goat blood, were preserved. A total of 77 engorged mosquito abdomens collected in the Comoros Islands and crushed on Whatman filter papers were tested with MALDI-TOF MS. Results The MS profiles generated from mosquito engorged abdomens crushed on Whatman filter papers exhibited high reproducibility according to the original host blood. The blood meal host was correctly identified from mosquito abdomens crushed on Whatman filter papers by MALDI-TOF MS. The MS spectra obtained after storage were stable regardless of the room temperature and whether or not they were frozen. The MS profiles were reproducible for up to three months. For the Comoros samples, 70/77 quality MS spectra were obtained and matched with human blood spectra. This was confirmed by molecular tools.Conclusion: The results demonstrated that MALDI-TOF MS could identify mosquito blood meals from Whatman filter papers collected in the field during entomological surveys. The application of MALDI-TOF MS has proved to be rapid and successful, making it a new and efficient tool for mosquito-borne disease surveillance.
- Published
- 2017