1. Proteomic analysis of cerebrospinal fluid from patients with idiopathic temporal lobe epilepsy.
- Author
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Xiao F, Chen D, Lu Y, Xiao Z, Guan LF, Yuan J, Wang L, Xi ZQ, and Wang XF
- Subjects
- Adolescent, Adult, Biomarkers cerebrospinal fluid, Blotting, Western, Cathepsin D cerebrospinal fluid, Cerebrospinal Fluid chemistry, Chromatography, Liquid, Clusterin cerebrospinal fluid, Cytokines cerebrospinal fluid, Electrophoresis, Gel, Two-Dimensional, Female, Humans, Lectins, C-Type analysis, Male, Mass Spectrometry, Middle Aged, Neoplasm Proteins cerebrospinal fluid, Proteomics methods, Superoxide Dismutase-1, Young Adult, Epilepsy, Temporal Lobe cerebrospinal fluid, Superoxide Dismutase cerebrospinal fluid, Talin cerebrospinal fluid, Vitamin D-Binding Protein cerebrospinal fluid
- Abstract
Proteomic analysis of cerebrospinal fluid (CSF) from patients with temporal lobe epilepsy (TLE) and controls was carried out using two-dimensional gel electrophoresis followed by liquid chromatography electrospray ionization tandem mass spectrometry. Five protein spots showed significant differential expression (p<0.05): vitamin D-binding protein (DBP) was elevated in the CSF of TLE patients whereas cathepsin D, apolipoprotein J, Fam3c, and superoxide dismutase 1 (SOD1) were decreased in the CSF of TLE patients. Additional six protein spots presented only in the CSF of epilepsy patients were identified as tetranectin (TN), talin-2, apolipoprotein E, immunoglobulin lambda light chain (IGL@), immunoglobulin kappa variable light chain 1-5 (IGKV1-5), and procollagen C-endopeptidase enhancer 1 (PCOLCE). Expression of DBP, SOD1 and talin-2 was validated by western blot. Our results may provide better understanding of the pathophysiologic mechanisms underlying epileptogenesis and possible epilepsy biomarkers.
- Published
- 2009
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