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Rapid hydrolysis of waste activated sludge (WAS) can be achieved by improving activities of extracellular enzymes under proper temperature conditions. As short-chain fatty acids (SCFAs) accumulation is always consumed by methanogens under anaerobic conditions, and considering that the micro-aerobic condition can inhibit the activities of methanogens, in this study, effects of temperature (55–75°C) on thermophilic micro-aerobic fermentation of WAS were investigated. Results showed that the highest soluble chemical oxygen demand (SCOD) yield was obtained at 60°C (4,407 ± 80 mg/L, 36 h), 2.0 times higher than that obtained at 75°C (2,180 ± 40 mg/L, 36 h), the corresponding hydrolysis rate was 0.6689 d−1. The highest SCFAs yield was 2,928 ± 12 mg COD/L at 60°C and 36 h, 4.9 times higher than that obtained at 75°C (594 ± 10 mg COD/L, 36 h). The analysis of SCFAs composition showed that acetic acid (HAc) accounted for the most percentage (>40%), followed by n-valeric (n-HVa) (20–25%), and propionic acids (HPr) (10–15%). Total suspended solids removal efficiency reached 18.7% after 192-h fermentation at 60°C. These results suggested that the optimal thermophilic micro-aerobic conditions for WAS hydrolysis and SCFAs accumulation from WAS were 60°C and 36-h fermentation time. [ABSTRACT FROM AUTHOR]

The effects of three widely-used surfactants on waste activated sludge (WAS) fermentation and microbial community structures were investigated. Rhamnolipid bio-surfactants (RL) showed more positive effects on WAS hydrolysis and acidification compared to chemosynthetic surfactants, such as sodium dodecylsulphate (SDS) and sodium dodecyl benzene sulfonate (SDBS). The highest SCOD and VFAs concentrations obtained with RL were 1.15-fold and 1.16-fold that of SDS, and up to 1.73 and 3.63 times higher than those obtained with SDBS. Pyrosequencing analysis showed that an evident reduction in bacterial diversity in surfactant-treated WAS. Moreover, acid-producing bacteria (such as Megasphaera and Oscillibacter ), detected with RL, were (6.8% and 6.4% in proportion) more abundant than with SDS, and were rarely found in SDBS and the control. The results also revealed that RL allowed efficient hydrolysis enhancement and was favorable to functional microorganisms for further acidification during WAS fermentation. [ABSTRACT FROM AUTHOR]

A novel pretreatment method combining freezing/thawing with Geobacillus sp. G1 was employed to pretreat waste activated sludge (WAS) for enhancing the WAS hydrolysis and subsequent short-chain fatty acids (SCFAs) production. Results showed that freezing/thawing combined with Geobacillus sp. G1 pretreatment achieved the maximal concentrations of soluble protein from 40 ± 6 mg COD/L (non-pretreated) to 1226 ± 24 mg COD/L (pretreated), and accumulated SCFAs concentration increased from 248 ± 81 mg COD/L to 3032 ± 53 mg COD/L. Excitation emission matrix (EEM) fluorescence spectroscopy revealed the highest fluorescence intensity (FI) of protein-like substances, which was the dominant fluorescent organic matters, indicating the synergistic effect of freezing/thawing and Geobacillus sp. G1 pretreatment on organics hydrolysis. High-throughput pyrosequencing analysis investigated that the abundance of bacteria responsible for WAS hydrolysis (such as Clostridium and Caloramator ) and SCFAs production (such as Parabacteroides and Bacterodies ) was greatly enhanced due to the novel pretreatment method used. [ABSTRACT FROM AUTHOR]

• 150 mgN/L of nitrate photolysis improved SCFAs production from WAS. • Nitrate increase (150∼375 mgN/L) has no significant promotion on SCFAs in photolysis. • ROS contributed more than RNS on WAS solubilization, hydrolysis and acidification. • Both AFB and NRB enriched for the high SCFAs production and complete denitrification. • Genes related to glycolysis, amino acid, acetate and nitrogen metabolism were enhanced. Nitrate photolysis has become an efficient, low-cost and promising technology for emerging contaminants removal, while its performance and mechanism for waste activated sludge (WAS) treatment is still unknown. This study innovatively introduced nitrate photolysis for WAS disintegration, and investigated the effect of nitrate addition (150–375 mg N/L) for short-chain fatty acids (SCFAs) production during anaerobic fermentation (AF). The results showed that nitrate photolysis significantly promoted the SCFAs production from WAS, and peaked at 280.7 mg/g VSS with 7-d fermentation with 150 mg N/L addition (150N-UV), which increased by 8.8–35.0 % and 10.7–23.3 % compared with other photolysis groups and sole nitrate groups. Effective release of the soluble organics was observed in the nitrate photolysis groups during AF, especially soluble proteins, reaching 1505.4 mg COD/L at 9 d in 150N-UV group, promoted by 7.0∼15.7 % than nitrate/nitrate photolysis groups. The model compounds simulation experiment further demonstrated the positive effect of nitrate photolysis on organics hydrolysis and SCFAs accumulation. The result of the radical capture and quenching verified the reactive oxygen species contributed more compared with reactive nitrogen species. Functional group analysis confirmed the effective bioconversion of the macromolecular organics during the fermentation. Moreover, the nitrate photolysis enhanced the enrichment of the functional consortia, including anaerobic fermentation bacteria (AFB), e.g., Fnoticella, Romboutsia, Gracilibacter and Sedimentibacter , and nitrate reducing bacteria (NRB), e.g., Acinerobacter and Ahniella. The macrogenetic analysis further revealed that glycolysis, amino acid metabolism, acetate metabolism and nitrogen metabolism were the dominating metabolic pathways during fermentation, and the abundance of the relevant genes were enhanced in 150N-UV group. [Display omitted] [ABSTRACT FROM AUTHOR]

Electro-fermentation (EF) has been extensively studied for recovering hydrogen and phosphorus from waste activated sludge (WAS), while was limited for the further application due to the low hydrogen yield and phosphorus recovery efficiency. This study proposed an efficient strategy for hydrogen and vivianite recovery from the simulated sludge fermentation liquid by sacrificial iron anode in EF. The optimum hydrogen productivity and the utilization efficiency of short chain fatty acids (SCFAs) reached 45.2 mmol/g COD and 77.6% at 5 d in pH 8. Phosphate removal efficiency achieved at 90.8% at 2 d and the high crystallinity and weight percentage of vivianite (84.8%) was obtained. The functional microbes, i.e., anaerobic fermentative bacteria, electrochemical active bacteria, homo-acetogens and iron-reducing bacteria were highly enriched and the inherent interaction between the microbial consortia and environmental variables was thoroughly explored. This work may provide a theoretical basis for energy/resource recovery from WAS in the further implementation. [Display omitted] • Enhanced hydrogen and vivianite recovery were realized in pH 8 via Fe mediated EF. • Appropriate iron release led to the higher crystallinity of vivianite in pH 8 group. • EAB, AFB and homo-acetogens preferred to enrich in pH 8 than other pH conditions. • The mutual symbiosis of microbes and environmental factors was the main mechanism in EF. [ABSTRACT FROM AUTHOR]

Waste activated sludge (WAS) is a promising phosphorus (P) source for fertilizer production; however, it is mainly enmeshed inside microbial cells or extracellular polymeric matrixes. Thus, efficient P release is a key goal. This study provided an effective route to simultaneously release organic P (OP) and harvest hydrogen from WAS via bio-electrolysis assisted with prefermentation system (AD_MEC). The clarification of P fractions in both solid and liquid phases and the underlying mechanism in this cascading system were explored. Free nitrous acid (FNA) was employed to enhance WAS hydrolysis. The results showed that hydrogen generation was effectively accelerated: 0.54 mL per gram sludge (in volatile suspended solids (VSS)) was produced from raw WAS (AD_MEC_RWAS), which increased to 1.27 mL/g VSS from FNA-pretreated WAS (AD_MEC_FWAS). The limited OP release by prefermentation was enhanced via MECs; additionally, it was further promoted by FNA, and the treatment time was also shortened. The maximum OP release efficiency reached 54.6% at 112 h (AD_MEC_FWAS). Additionally, non-apatite and inorganic apatite P may regenerate in MECs due to the localized decrease in pH caused by hydrogen generation. This work may provide a scientific basis to enhance P and hydrogen co-recovery from WAS in forthcoming implementation. Image 1 • H 2 recovery and P release from WAS was achieved in cascading conversion system. • The limited OP release in prefermentation was accelerated via bio-electrolysis. • FNA played a positive effect on H 2 recovery and OP release from WAS. • Underlying mechanism of cascading conversion system for P release was explored. [ABSTRACT FROM AUTHOR]

Aiming to strengthen the performance of waste activated sludge digestion, pretreatment is a prerequisite for keeping operations within an industrially acceptable time- frame. In this study, the performance of microaeration coupled with thermophilic (MT) pretreatment on waste activated sludge solubilization and acidification was investigated. The results showed that the maximum soluble organics concentrations, including soluble proteins and carbohydrates, reached 2290 mg COD/L in 24 h when the ventilation rate and temperature were 0.05 vvm and 70 °C. SDS-polyacrylamide gel electrophoresis (SDS-PAGE) test on pure-culture ( E.coli ) and scanning electron microscopy analysis indicated that MT pretreatment effectively destroyed microbial cell wall and resulted in an increase in soluble proteins. Polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) analysis revealed that MT pretreatment reduced the diversity of the bacterial community during pretreatment. Along with the dissolution of a large quantity of organic matter, microbial species such as Lactococcus and Methyloversatilis bloomed at the end of acidification period, which contributed to SCFAs production. This study revealed that MT pretreatment facilitated the WAS hydrolysis efficiency and enhanced SCFAs (3149 mg COD/L with fermentation for 96 h) production, especially for acetic acid (50%) accumulation, which provides a new perspective for the application and recovery of sludge resources. [ABSTRACT FROM AUTHOR]

Current studies have employed various chemicals for disintegrating and hydrolyzing microbial cells in waste activated sludge (WAS). However, a comprehensive process assessment over the whole anaerobic digestion process has seldom been proposed. Besides, the characterization of microbial community responses to these chemicals is not well understood. In this study, the effects of five typical chemicals: solubilizer (β-cyclodextrin, CD), alkaline (NaOH), peroxide (peracetic-acid, PA), biological (rhamnolipid, RL) and chemical (sodium dodecylsulphate, SDS) surfactants on WAS digestion were examined. Higher efficiencies of WAS solubilization, hydrolysis and acidification were achieved by CD treatment, followed by RL and SDS. Methanogenesis was also strongly chemicals-dependent. Shifts in microbial community structure were observed in all chemical-pretreated WAS. The community in RL, CD and PA was dominated by microorganisms that anaerobically hydrolyze organics to acids, while that in NaOH and SDS was mainly associated to biogas production. This study proved that the overall performance of WAS digestion was substantially depended on the initial chemical pretreatments, which in turn influenced and was related to the microbial community structures. Although the economic advantage might not be clear yet, the findings obtained in this work may provide a scientific basis for the potential implementation of chemicals for WAS treatment. [ABSTRACT FROM AUTHOR]

The effects of chemosynthetic and biological surfactants on accelerating hydrogen generation from waste activated sludge (WAS) is investigated in a short-cut fermentation-bioelectrochemical system. The specific experiments are conducted in a series of completely stirred tank reactors (CSTRs) and single-chamber microbial electrolysis cells (MECs). Results shows that rhamnolipid (RL) lead to a VFAs yield 1.16-fold and 3.63-fold higher over with sodium dodecylsulphate (SDS) and sodium dodecyl benzene sulfonate (SDBS) treatments in CSTRs on 72 h. By contrast, the corresponding conversion efficiency of methanogenesis is inhibited (0.18 ± 0.03% versus 1.89 ± 0.15% (SDS) and 6.63 ± 0.77% (SDBS)), which is beneficial for subsequent hydrogen production in MECs. The distribution of the acidogenesis metabolites is also affected by the types of surfactants, reflected on cascade changing of hydrogen production. Highest hydrogen yield is 12.90 mg H 2 g −1 VSS in RL-MECs, which is larger than all values that have been reported for fermentation and single-chamber MECs. Current and electrochemical impedance spectroscopy clearly demonstrate the important role of RL treatment in electron/proton transfer and the internal resistance decrease. This study demonstrate the sustainability and attractiveness of WAS short-cut fermentation-elelctrohydrogenesis, providing a sound basis for sludge stabilization and bioenergy recovery. [ABSTRACT FROM AUTHOR]

To improve anaerobic digestibility of waste activated sludge (WAS), a novel treatment was explored by a plant-derived biosurfactant (saponin biosurfactant (SB)). SB showed positive effects on WAS hydrolysis and acidogenesis with increasing concentration, but was insignificant at dosage >0.20 g SB/g total suspended solids (TSS). Soluble protein and carbohydrate concentrations reached 2029 ± 28 and 343 ± 52 mg chemical oxygen demand (COD)/L (0.20 g SB/g TSS, 48 h), which were 4.77-fold and 5.87-fold higher than that without treatment. Volatile fatty acids (VFAs) bioproduction showed a 3.51-fold increase in 72 h. Moreover, acetic acid increased already after 2 h with SB treatment, but after 8 h in the control, indicating that SB accelerated syntrophic acetogenesis from butyric acid degradation to acetic acid. Additionally, methane production was enhanced at lower dosage (<0.05 g SB/g TSS) and inhibited at higher dosage (>0.10 g SB/g TSS), with 63.0 and 20.3 mL/g volatile suspended solids (VSS), respectively, while it was 46.5 mL/g VSS in the control. The results obtained are in good agreement with previous ones obtained with chemosynthetic surfactants. Moreover, SB has advantages of easy production and sustainability of supply, which should promote the implementation for WAS treatment. [ABSTRACT FROM AUTHOR]

Highlights: [•] A novel strategy for improving VFAs bioproduction from waste activated sludge (WAS) was developed. [•] WAS hydrolysis, VFAs production and protein degradation were improved. [•] A synergistic effect occurred with co-digesting WAS and ABS. [•] FTIR and 3D-EEM were successfully employed to explore the mechanism. [ABSTRACT FROM AUTHOR]

Highlights: [•] SCFAs yield was enhanced by co-digesting waste activated sludge and corn stover. [•] Corn stover proportion in feedstock determined the acidification product spectrum. [•] Corn stover addition improved the hydrolysis and acidification of particulate organics. [•] Optimal feedstock proportion was 65%WAS:35%CS from carbon balance and cost estimation. [Copyright &y& Elsevier]

Free nitrous acid (FNA) has been recently reported to be an effective and eco-friendly inactivator for waste activated sludge (WAS), while the limited decomposition of the extracellular polymeric substance (EPS) matrix hampers resource recovery from WAS. This work employed peracetic acid (PAA) to assist FNA and explored the contribution of co-pretreatment to hydrogen recovery in a prefermentation-bioelectrolysis cascading system. The results showed that co-pretreatment led to approximately 8.8% and 20.4% increases in the exfoliation of particulate proteins and carbohydrates, respectively, from tightly bound EPS (TB-EPS) over that of sole FNA pretreatment. Electron paramagnetic resonance analysis verified that the synergistic effect of FNA, PAA and various generated free radicals was the essential process. This effect further promoted the accumulation of volatile fatty acids (VFAs) after 96 h of prefermentation, and the peak concentration in co-pretreated WAS (AD-FPWAS) was approximately 2.5-fold that in sole FNA-pretreated WAS (AD-FWAS). Subsequently, the cascading utilization of organics in the bioelectrolysis step contributed to efficient hydrogen generation. A total of 10.8 ± 0.3 mg H 2 /g VSS was harvested in microbial electrolysis cells (MECs) fed with AD-FPWAS, while 6.2 ± 0.1 mg H 2 /g VSS was obtained from AD-FWAS. X-ray photoelectron spectroscopy (XPS) revealed the effective decomposition of the phospholipid bilayer in the cytomembrane and the transformation of macromolecular organics into VFAs and hydrogen in the cascading system. Further microbial community analysis demonstrated that co-pretreatment enhanced the accumulation of functional consortia, including anaerobic fermentative bacteria (AFB, 28.1%), e.g., Macellibacteroides (6.3%) and Sedimentibacter (6.9%), and electrochemically active bacteria (EAB, 57.0%), e.g., Geobacter (39.0%) and Pseudomonas (13.6%), in the prefermentation and MEC steps, respectively. The possible synergetic and competitive relationships among AFB, EAB, homo-acetogens, nitrate-reducing bacteria and methanogens were explored by molecular ecological network analysis. From an environmental and economic perspective, this promising FNA and PAA co-pretreatment approach provides new insight for energy recovery from WAS biorefineries. Image 1 • PAA, FNA and their intermediates led to effective EPS decomposition. • PAA + FNA pretreatment boosted 2.5-fold VFAs and 1.7-fold H 2 recovery versus sole FNA. • XPS confirmed the destruction of phospholipid bilayer and organics transformation. • 28% AFB in prefermentation and 57% EAB in bioelectrolysis were enriched. • Synergetic relationships of AFB, EAB, homoacetogen, NRB and methanogen were explored. [ABSTRACT FROM AUTHOR]