1. The Asia Oceania Human Proteome Organisation Membrane Proteomics Initiative. Preparation and characterisation of the carbonate-washed membrane standard.
- Author
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Peng L, Kapp EA, Fenyö D, Kwon MS, Jiang P, Wu S, Jiang Y, Aguilar MI, Ahmed N, Baker MS, Cai Z, Chen YJ, Van Chi P, Chung MC, He F, Len AC, Liao PC, Nakamura K, Ngai SM, Paik YK, Pan TL, Poon TC, Salekdeh GH, Simpson RJ, Sirdeshmukh R, Srisomsap C, Svasti J, Tyan YC, Dreyer FS, McLauchlan D, Rawson P, and Jordan TW
- Subjects
- Animals, Asia, Carbonates, Humans, Membrane Proteins standards, Mice, Oceania, Organizations, Proteomics methods, Cell Membrane chemistry, Intracellular Membranes chemistry, Membrane Proteins chemistry, Proteome, Proteomics standards
- Abstract
The Asia Oceania Human Proteome Organisation (AOHUPO) has embarked on a Membrane Proteomics Initiative with goals of systematic comparison of strategies for analysis of membrane proteomes and discovery of membrane proteins. This multilaboratory project is based on the analysis of a subcellular fraction from mouse liver that contains endoplasmic reticulum and other organelles. In this study, we present the strategy used for the preparation and initial characterization of the membrane sample, including validation that the carbonate-washing step enriches for integral and lipid-anchored membrane proteins. Analysis of 17 independent data sets from five types of proteomic workflows is in progress., (Copyright © 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)
- Published
- 2010
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