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Unlabelled: To compare yield, alisol content of Alisma orientalis planted at different ecological climatic regions, and explore further the impact of environmental factors on the yield and quality., Method: Different local varieties were planted at varing ecological climatic conditions. Diameter, yield was measured after harvest, the contents of 23-acetyl alisol B and 24-acetyl alisol A were quantitatively analyzed by HPLC., Result: The result revealed that ecological condition had significant impacts on yield and alisol content. Yield of MeiShan was the highest which was up to 1 200.72 kg x hm(-2). The contents of 23-acetyl alisol B and 24-acetyl alisol A of A. orientalis cultivated in Dujiangyan were significantly higher than those of other regions, the values were up to 4.222, 2.727 g x kg(-1), respectively. 23-acetyl alisol B content was positively correlated with 24-acetyl alisol A content (P < 0.01). The diameter was positively correlated with yield (P < 0.01)., Conclusion: Considering yield and medicinal ingredients, Dujiangyan may be the most suitable region to plant A. orientalis.

Objective: To establish chromatographic fingerprint of Sichuan native medicinal plant Alisma plantago-aquatica by RP-HPLC for the quality control., Method: The gradient elution mode was applied in chromatographic separation, and data were analyzed by "Computer Aided Similarity Evaluation" software to compare the quality of A. plantago-aquatica samples from different habitats., Result: The HPLC chromatographic fingerprinting of A. plantago-aquatica with 26 characteristic peaks was established from 17 lots of A. plantago-aquatica samples, peak 16 and 22 were identified as 24-acetyl alisol A and 23-acetyl alisol B, respectively., Conclusion: The chromatographic fingerprinting of A. plantago-aquatica with high specificity can be used to control its quality and assure lot to lot consistency. The RP-HPLC fingerprint method is repeatable, feasible in analysis of A. plantago-aquatica.