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The current study aimed to explore the effect of Brain-Based Learning on students' intrinsic motivation (IM) to learn and perform in mathematics. Owing to the educational implications of Neuroscience, the researchers planned the mixed-methods experimental study with a convergent parallel research design. The participants were eighth-graders enrolled in a boy's public secondary school in the district Kasur of Pakistan in the academic year 2021-2022. Students were taught mathematics using the traditional lecture method in the baseline phase (A) and withdrawal phase (A). In contrast, in the treatment phase (B), they were taught mathematics with activities based on the BBL approaches and principles. The mathematics motivation scale and observation (field notes) were used to collect quantitative and qualitative data simultaneously. Data were analysed using one-way repeated measure ANOVA and thematic analysis. The researchers revealed that BBL significantly affects students' IM to learn and perform in mathematics. After qualitative analysis, it was found that visual story-telling, role-playing, i-Think maps, back-to-board, and Kick Me Poison Box are the most effective BBL-based activities which keep them engaged, provide emotional support and contribute to students' IM to learn and perform in mathematics. The teachers were suggested to develop BBL-based activities to teach mathematics and to influence students' psychology in the schools.

This study problematises the discourses of Pakistan's national curriculum textbooks to investigate how they represent Pakistan's indigenous languages/cultures through the prism of religion to constitute secondary school students' particular postcolonial linguistic identities. It also draws on the perspectives of 12 teachers and 424 students to record their responses. Taking insights from Paulo Freire's "cultural invasion," Robert Blauner's "internal colonisation" and selected postcolonial perspectives, the study notes a dynamic interplay of the sampled textbooks, schoolteachers and the school as a site of discursive social practices. Jointly, they position students within an exclusionary homogenous Urdu-loving group--a language which is represented as Arabic's tributary. Indigenous languages are recognised, however, as an adjunct to Arabic and Urdu, emphasising their role in the proselytisation of Islam in the region. Similarly, fine arts and indigenous cultural festivals are represented from a particular religious lens. The students strongly identify with this curious case of "internal colonisation" and "cultural invasion," and support it. They exhibit self-righteousness and aversion towards linguistic/cultural pluralism and minorities, which entails implications for Pakistan's national cohesion and educational parlance of equity and inclusivity.

This study analyses discourses of Pakistan's national curriculum textbooks for grades 9-12 in the context of developing students' national belonging vis-à-vis inclusive education and global interdependence. Drawing on teachers' interviews and students' focus groups and participatory tools, it also problematises teachers' classroom practices and resulting students' responses in this backdrop. Theorising national belonging as a discursive social practice, the study benefits from Foucault's approach to discourse analysis. The findings highlight a complex interplay of several education system actors for constructing students' national belonging, emphasising an essential relationship between Pakistan and a particular version of Islam. Discursively positioned, the students perceive the world as Muslim versus Non-Muslim and display self-righteousness, evincing strong ethnocentricity towards fellow non-Muslims. The study underlines the implications for not employing education for fostering inclusivity and international collaboration and its consequences for long-term global sustainable development goals (SDGs), 2030.

Objectives: Aminoacidopathies are inborn errors of metabolism (IEMs) that cause intellectual disability in children. Luckily, aminoacidopathies are potentially treatable, if diagnosed earlier in life. The focus of this study was the screening of aminoacidopathies in a cohort of patients suspected for IEMs. Methods: Blood samples from healthy (IQ > 90; n = 391) and intellectually disabled (IQ < 70; n = 409) children (suspected for IEMs) were collected from different areas of Northern Punjab, Pakistan. An analytical HPLC assay was used for the screening of plasma amino acids. Results: All the samples (n = 800) were analyzed on HPLC and forty-three out of 409 patient samples showed abnormal amino acid profiles mainly in the levels of glutamic acid, ornithine and methionine. Plasma concentration (Mean ± SD ng/mL) were significantly high in 40 patients for glutamic acid (patients: 165 ± 38 vs. controls: 57 ± 8, p < 0.00001) and ornithine (patients: 3177 ± 937 vs. controls: 1361 ± 91, p < 0.0001). Moreover, 3 patients showed abnormally high (53.3 ± 8.6 ng/mL) plasma levels of methionine. Conclusion: In conclusion, biochemical analysis of samples from such patients at the metabolites level could reveal the underlying diseases which could be confirmed through advanced biochemical and genetic analyses. Thus, treatment to some of such patients could be offered. Thus burden of intellectual disability caused by such rare metabolic diseases could be reduced from the target populations.

This qualitative study analyses the discourses of "Islamic Studies" textbook to problematise its role in schoolchildren's radicalisation, and normalisation of violence, in the post 9/11 situation in the Taliban's stronghold, South Waziristan. The textbook findings are further substantiated by the field data collected from 40 students of grades 9 and 10, who studied the sampled textbook in the post 9/11 situation. Students' perspectives, which relate their pedagogical experiences of the textbook in public schools, were gathered employing 16 in-depth individual interviews and 8 focus group discussions. Taking insights from Apple and Ball's concepts of 'legitimate knowledge' and 'normalisation' respectively, the findings suggest that the sampled textbook strongly inspired a particular kind of "Jihad" (holy war), created hate-based 'believers/Muslims' versus 'non-believers/"Kuffar'" binaries, and profiled communities in opposition to each other. The students strongly identified with the discursive textbook representations, and participated in "Jihad" both as the Taliban commanders and foot soldiers. Some of them died young, however, the majority survived of which some recanted. Notwithstanding external influences, the study underlines the role that the textbook discourses and their pedagogical practices in schools played in radicalising the whole generation of schoolchildren and normalising the Taliban fronted violence in Pakistan's tribal areas.

Purpose: Sustainability in higher education has gained the attention of researchers and academia; however, there is still a need to explore and assess it from different perspectives that are unexplored. This study aims to evaluate and report sustainability and sustainable development in the higher education sector of Pakistan. Design/methodology/approach: There are 195 recognized and registered degree awarding institutes in Pakistan. A survey-based study was designed using self-administered questionnaires distributed among the deans of 145 Higher Education Institutions (HEIs) using a simple random sampling technique. Responses were recorded using five-point Likert scale. In this study, 74 HEIs participated while 69 responses were complete in all respects and used for data analysis. Responses were recorded and analyzed using descriptive statistics. Findings: The results show little evidence of sustainability in all areas of HEIs (including; curriculum, research, faculty, stakeholder's engagement and governance). Sustainability of campus (operations) was evident and, to a certain extent, apparent in outreach activities. The core objective of HEIs is teaching and research, however, HEIs in Pakistan have yet to accommodate sustainability in these core areas. Sustainability in HEIs of Pakistan is in the preliminary and the introductory stage. For the attainment of sustainability in HEIs, comprehensive planning and internal governance are required. Research limitations/implications: This study was based on a survey to report the overall situation of sustainability in Pakistan's HEIs. Therefore, it has the limitation of being a descriptive study. Nevertheless, this study contributed to the body of knowledge by reporting sustainability practices from the Pakistani context. This study helped identify critical aspects of sustainability that require the attention of both regulatory authority and top management. Originality/value: This is a comprehensive study based on extensive research survey techniques to present and report sustainability in higher education in Pakistan. The data collected represents the major HEIs in Pakistan and has contributed to the body of knowledge by presenting the contemporary and contextual situation of sustainability in HEIs of Pakistan.

The purpose of this research was to understand the automation of Higher Education Institutes (HEIs), and to evaluate the automated process from the perspective of developing country. A single case study of City University of Science and Information Technology (CUSIT), Pakistan was selected as a unit of analysis. Mix methodology was used in this research. Semi-structured in-depth interviews were conducted from the top management as a primary source while secondary data regarding usage and functions of LMS was collected for the period of 2 years (four semesters Fall-2017-Spring 2019). Data from both sources was utilized, and triangulated for case building and analysis. It was found that CUSIT has a state-of-the-art automated infrastructure, management information system (MIS) and learning management system (LMS) which facilitates faculty, staff and administration. LMS provides an integrated and digital platform to key stakeholders particularly to the teachers for sharing course outlines, lesson plan, assignment generation and submission, announcements and generating assessment reports. Similarly, the student gets access to all course material, assignments, assessment report, notices, and other relevant information at any point of time. However, the system lacks some of the major E-learning features with limited support to the large file size, real time learning, online classes, real time feedback and query system etc. Furthermore, this study identified few seldomly used functions of automated system as well as few duplications and operational activities which requires consideration by the management. The case of CUSIT proves to be a practical case for all those universities who are facing challenges due to corona virus pandemic and are looking for a solution to continue education through distance learning and e-learning platforms. This study supports the automation of HEIs and provides lesson learned for the academia and management of HEIs by identification of success features as well as limitations of the system.

Purpose: The integration of sustainability is more matured in the corporate sector. However, the topic received less attention in higher education institutions (HEIs). Therefore, this study aims to conceptualize an integration framework and proposes sustainability integration and implementation index in the HEIs. Design/methodology/approach: The index was developed from previous literature. For the implementation of the index, three universities were selected. The required data collected from websites, internal documents and interviews with the high officials of these universities. Findings: Sample universities have somehow inclination toward the broad agenda of sustainable development, but still, they lack integration, implementation and reporting of their sustainable practices. Moreover, the departments have positive and practical intent toward sustainability especially regarding the environment, designing curriculum, teaching, research activities and volunteerism. However, in assessment, the social and economic contributions of these universities revealed to be somewhat mild. The findings also evidenced heterogeneity among the sample institutions to follow the said agenda which reflects a lack of awareness and policy or a centralized or regulatory push from the top. Research limitations/implications: The study is limited to three universities in Khyber Pakhtunkhwa, Pakistan. Hence, in the future, the framework and index may be tested in other universities of the country or region. Nevertheless, the study carries theoretical significance in the literature of sustainability. Practical implications: The study has practical implications for academia, regulatory bodies and universities to integrate, implement and report sustainability in HEIs. Originality/value: The study proposes and validates the sustainability integration and implementation index in HEIs. The index is unique and has originality or value particularly for HEIs of the developing countries.

This paper examined students' performance through diverse assessments in MS first semester curriculum module in a women university. The module "Curriculum models and theories" incorporated three different assessments: a home assignment, a classroom presentation and a final term paper. A three step approach using mixed research method was used to investigate students' responses to the different assessment tools. First a quantitative analysis using descriptive statistics was conducted on the data from students' marks. Second, an examination of students' perceptions about different assessment was carried out through a questionnaire. Last a qualitative study was conducted using semi structured interviews. The findings revealed that majority of the students preferred more structured tasks having clear aligned learning outcomes. Familiarity with assessment types was also preferred by many students. The present study suggests that by making changes in assessment design and using more formative feedback, students' learning experience across diverse assessment tools can be enhanced.

This qualitative study problematizes the interplay between Pakistan's national curriculum textbooks and students' militaristic national identity constructions in six state-schools. Drawing on field-data collected employing interviews with twelve teachers and focus-groups and participatory tools with four-hundred and twenty-four students, it also analyses study-participants' interaction with these discourses and students' reactions "vis-à-vis" their teacher-mediated textbook positioning. Taking insights from Synder's and Johnston's concept of 'strategic culture' and Foucault's 'technologies of power and self', the findings suggest Pakistani students' militaristic national identity constructions in schools, portraying India as an existentialist threat to Pakistan. The textbooks glorify the India/Pakistan wars, heighten the feeling of insecurity amongst schoolchildren, and through that, build a heroic image of the Pakistan Army. The teachers reinforce these images in classrooms. Constituted under the influence of these "technologies of power," students exude strong hostility towards India and adoration for the Pakistan Army, hence functioning as vehicles to further the militaristic approach of Pakistan's 'regime of truth'. The study illustrates how Pakistan's increasingly strengthening "strategic culture" has subverted Pakistan's civil institutions and served the vested interests of the military's high command. Similarly, it highlights the dangers/implications involved in using national education for shaping vulnerable schoolchildren's militaristic national identities in schools.

The study examined the factors that affect girls' education at secondary school level in Karak District, Khyber Pakhtunkhwa (Pakistan). All the female heads, teachers and students serving and studying at secondary school level in Karak District constituted the population of the study. The study was delimited to only 30 girls' secondary schools in the same district. In order to ensure adequate representation of the population, 30 heads, 120 teachers and 480 students were selected randomly as a sample. Quantitative descriptive research designed was used. Data was collected through self-developed semi-structured questionnaire designed on five Point Likert Scale. Statistical tools i.e., percentage and chi-square were used for data analysis. It was concluded that there were various factors that affect girls' education. These were: unpunctuality of teachers; unfeasibility of school buildings; poor financial status of the parents; lack of basic facilities; marriage at early age; lack of parental attention; negative attitudes of parents about girls' education; illiteracy of the parents; lack of competent teachers; lack of basic facilities for teachers; lack of proper security arrangement; and long distance to school, etc. Based on findings, it was recommended that negative attitude towards female education should be discouraged through mass media. Early marriage culture should be banned. Secondary education should be made free and a special programme of scholarships should be lunched for female students. School buildings should be constructed on feasible and suitable places so that majority of the girls may be beneficialized.

This study investigates Pakistan's secondary school children's constructions of their national identity in a Pakistani school in Dubai by drawing on data collected from students and teachers from the case school and analysing national curriculum textbooks used in the school. Informed by Foucault's concepts, the article problematises how the curriculum textbooks are employed as a "technology of power" for inculcating national consciousness in the students. The findings suggest that Pakistan's national curriculum textbooks deploy a specific version of Islam as a major "technology," which then influences other national identity signifiers in the textbooks for shaping students' national identity. The school affords a crucial space for the complex interplay of these "technologies," which construct students' ethnocentric national identities, encouraging social polarisation. This has implications for Pakistan's national social cohesion as well as the potential for subverting international peaceful coexistence and working relationships, particularly in the selected overseas study context.

Background: The realm of medical laboratory technology (MLT) training and education is unstructured in Pakistan. The primary challenge currently confronting the workforce in MLT is the absence of standardized curricula and assessments in education and training. This was an exploratory study aiming to inquire experiences of trainees, alumni and teaching coordinators regarding the technologist training program in a single institute at Pakistan. Methods: To gain an in depth understanding of MLT program, three focus group discussions (FGDs) were held at Department of Pathology and Laboratory Medicine, Aga Khan University, Pakistan during Feb-April 2024. A team of pathologists and educationists developed interview guides for FGDs in English. Interviews were bilingual, transcribed verbatim and coded using thematic analysis. Participants included current trainees, alumni, teaching and learning coordinators and moderators for the interview. Results: A total of 29 participants were engaged; these included current MLT trainees (n = 10), alumni (n = 10), and teaching/learning coordinators and sectional supervisors (n = 9). Five main themes emerged from the analysis of FGDs: (Scott MG, Rifai N, Smith B, Oellerich M, Panteghini M, Apple F et al. The changing face of laboratory medicine: a more service and less academically oriented profession? 2015;61(2):322-9.) Recognition of key features of the MTT program, (Ferraro S, Braga F, Panteghini MJCC, Medicine L. Lab Med new Healthc Environ. 2016;54(4):523–33.) Evaluating curriculum design, (Waheed U, Ahmad M, Wazeer A, Saeed M, Saba N, Rasheed FJMJMS. Medical laboratory science education; shaping competent and skilled healthcare professionals. 2023;1(1):58–63.) Teaching and learning strategies, (Ned-Sykes R, Johnson C, Ridderhof JC, Perlman E, Pollock A, DeBoy JM. Competency guidelines for public health laboratory professionals. 2015.) Addressing the need to improve assessment methods, and (Linder RJJM, Education B. Educating medical laboratory technologists: revisiting our assumptions in the current economic and health-care environment. 2012;13(2):150-4.) Navigating the transition from a trainee to a competent technologist. Conclusion: Our investigation demonstrated its potential as a valuable needs assessment study, highlighting key strengths, drawbacks, and challenges of the existing MTT program. Importantly, these findings at our institute can inform further research efforts to design competency-based MLT education and training programs in Pakistan. [ABSTRACT FROM AUTHOR]

A continuous supply of electricity is necessary to maintain an acceptable standard of life, and the power distribution system's overhead line components play a crucial role in this matter. In Pakistan, identifying defective parts often necessitates human involvement. An unmanned aerial vehicle was used to gather a collection of 10,343 photos to automate this procedure. Using supervised and unsupervised machine learning methods, a number of automated anomaly detection systems were created. Support vector machine, random forest, VGG16, and ResNet50 were used as supervised machine learning models, and a convolutional auto‐encoder was used as the unsupervised machine learning model. VGG16 achieved the best accuracy of 99.00% while random forest achieved the worst accuracy of 72.49%. The convolutional auto‐encoder was successful in distinguishing between normal and abnormal components. The aforementioned machine learning models can be put on unmanned aerial vehicles to immediately identify defective parts. [ABSTRACT FROM AUTHOR]

Physicochemical properties of water influence planktonic diversity and distribution, which is essential in obtaining basic knowledge of aquatic biodiversity. Thus current study aims to investigate the spatiotemporal diversity, abundance ratio, and distribution of phytoplankton species and their association with water quality parameters of Chashma Lake, Pakistan. During the study period from 2018 to 2019, we measured 13 physicochemical parameters across three selected sampling sites (S1, S2, and S3) in Chashma Lake, revealing both spatial and temporal variability. Dissolved oxygen (DO) was higher in S3, while S1 exhibited higher alkalinity levels, carbon dioxide, phosphorus, and chloride levels. The study identified 77 phytoplankton species grouped into five taxonomic categories, with Cyanobacteria dominating (39.90%), followed by Chlorophyta (33.4%) and Bacillariophyta (24.88%). Euglenozoa and Ochrophyta were less abundant (1.3% and 0.41%, respectively). Spatial variations in phytoplankton distribution were noted, with Chlorophyta being more abundant at S2, Bacillariophyta and Cyanobacteria at S1, and Euglenozoa dominating at S3. Canonical Correspondence Analysis (CCA) revealed the influence of various physicochemical parameters on phytoplankton distribution. This comprehensive study provides valuable insights for the ecological assessment and monitoring of water bodies. It is recommended that continuous monitoring is required to capture long-term trends, further explore the specific environmental drivers impacting phytoplankton dynamics, and consider management strategies for maintaining water quality and biodiversity in Chashma Lake. [ABSTRACT FROM AUTHOR]

The present study explored the nutritional composition, phytochemicals analysis, and antioxidant capacity of two indigenous varieties of red and green water chestnut (WCN) fruit grown in Pakistan. Accordingly, this study was designed to investigate the proximate composition (moisture, ash, fiber, proteins, fat, and energy), physicochemical properties (pH, °Brix, and glycemic index), minerals, and vitamins. The methanolic extracts of WCN fruits were explored for phytochemicals (total phenolic and flavonoid content), and antioxidant potential was examined in vitro by 1,1-diphenyl-2-picrylhydrazyl radical scavenging capacity (DPPH) and Ferric reducing antioxidant power (FRAP). Quantitative determination of mineral (sodium, potassium, calcium, phosphorus, iron, manganese, copper, and zinc) and vitamin (vitamin C, vitamin B6, vitamin B2, vitamin B3, vitamin A, and β-Carotene) composition was also assessed. Based on the findings, the proximate compositions of WCN green and red varieties varied greatly as WCN green contained significantly higher protein (1.72%), fat (0.65%), dietary fiber (2.21%), moisture (70.23%), ash (1.16%), and energy content (112.8 Kcal) than WCN red. In WCN green, the macro–micromineral concentrations were significantly higher than WCN red. Among the minerals analyzed, potassium was the most abundant mineral found in both varieties. Levels of vitamin C, B6, A, and β-Carotene were significantly higher in WCN green. In this study, methanolic extract showed higher extraction efficiency than acetone, ethanol, and distilled water. WCN green had a significantly higher quantum of total phenolic (91.13 mg GAE/g) and total flavonoid (36.6 mg QE/g) and presented significantly higher antioxidant activity than the WCN red. This study showed that, among both varieties, WCN green extract has therapeutic potential against free radical mediated health conditions and suggested the potential use of this fruit as a source of natural antioxidants in nutraceuticals. [ABSTRACT FROM AUTHOR]

Objective: To study the adjuvant per-operative epidural administration of Triamcinolone in lumbar disc surgery to aleviate post-operative pain. Study Design: Quasi-experimental study. Place and Duration of Study: Department of Neurosurgery, Combined Military Hospital, Rawalpindi Pakistan, from Oct 2021 to Sep 2022 Methodology: Hundred patients aged 20 to 80 years, of either gender, with a single-level prolapsed intervertebral disc and nerve root compression were included and underwent unilateral single-level decompression. A pre-operative MRI scan of the lumbo-sacral spine was done to confirm the diagnosis. Al patients initialy received conservative management (analgesics and physiotherapy) but failed to respond to it. They were divided into two groups by the lotery method. In Group A, an epidural steroid was administered, while Group B acted as the control. Pain was recorded pre-operatively at 24 hours and then at 1 and 2 weeks post-operatively using the Visual Analogue Scale. Results: The pre-operative median VAS of Groups A and B was not significant at pre-operation. After 24 hours of operation, the median VAS of Group A patients was VAS 3 (1), while in Group-B it was 2 (1), (p-value of 0.001). After 1 week, the median VAS of Group-A patients was 2 (2) and Group-B was 3 (1), with a significant p value of 0.024. After 2 weeks, the median VAS of Group-A patients was 1 (1) and Group B was 2 (1), with a significant p value of 0.015. Conclusion: Intra-operative epidural and foraminal administration of steroids in single-level lumbar disc surgery significantly reduces pain in the immediate postoperative period but has no benefit in the folow-up period. [ABSTRACT FROM AUTHOR]

Globally, Group A rotavirus (RVA) is the leading cause of acute gastroenteritis in children under 5 years old, with Pakistan having the highest rates of RVA‐related morbidity and mortality. The current study aims to determine the genetic diversity of rotavirus and evaluate the impact of Rotarix‐vaccine introduction on disease epidemiology in Pakistan. A total of 4749 children, hospitalized with acute gastroenteritis between 2018 and 2020, were tested at four hospitals in Lahore and Karachi. Of the total, 19.3% (918/4749) cases were tested positive for RVA antigen, with the positivity rate varying annually (2018 = 22.7%, 2019 = 14.4%, 2020 = 20.9%). Among RVA‐positive children, 66.3% were under 1 year of age. Genotyping of 662 enzyme‐linked immuno sorbent assay‐positive samples revealed the predominant genotype as G9P[4] (21.4%), followed by G1P[8] (18.9%), G3P[8] (11.4%), G12P[6] (8.7%), G2P[4] (5.7%), G2P[6] (4.8%), and 10.8% had mixed genotypes. Among vaccinated children, genotypes G9P[4] and G12P[6] were more frequently detected, whereas a decline in G2P[4] was observed. Phylogenetic analysis confirmed the continued circulation of indigenous genotypes detected earlier in the country except G9 and P[6] strains. Our findings highlight the predominance of G9P[4] genotype after the vaccine introduction thus emphasizing continual surveillance to monitor the disease burden, viral diversity, and their impact on control of rotavirus gastroenteritis in children. [ABSTRACT FROM AUTHOR]

Calcium and Vitamin D deficiency is highly prevalent in Pakistan. Both micronutrients have a significant role in human growth and development. Supplementation becomes essential when the available natural sources like food and sunlight, cannot replenish the deficiencies. Nevertheless, it is critical that while prescribing Calcium or Vitamin D supplements, physicians should know the composition and specific application of each supplement. This review explores the different types of Calcium and Vitamin D pharmaceutical supplements available in the market their composition, indication and contraindications. Data of Calcium and Vitamin D supplements has been colected by Pakistani drug sites databases, including 'Drug Infosys (www.druginfosys.com)', 'Dawaee.pk', 'Sehat.com.pk' and 'Medicine Net' by pharmacists and orthopaedists. The commonly prescribed supplements are Cholecalciferol (Vitamin D) and Calcium Carbonate (Calcium). Over time, an increase in market share of Vitamin D and Calcium supplementations is due to their over-the-counter availability, increased physicians' prescriptions, and self-prescribed use by the population. There is a need to develop national guidelines and policies to safeguard the population's health at large. [ABSTRACT FROM AUTHOR]

Objective: To evaluate the frequency of Postpartum depression among COVID-19 affected mothers. Study Design: Comparative cross-sectional study. Place and Duration of Study: Obstetrics and Gynaecology Department of PEMH, Rawalpindi Pakistan, from Jun to Aug 2020. Methodology: A validated questionnaire was used to colect patient data regarding demographic profile and after which questions were asked to calculate Edinburgh Postnatal Depression Score from both COVID-19 positive and negative mothers, 6 weeks after delivery. A score of more than 12 was considered statisticaly significant for postnatal depression. Results: Total number of women included in the study were 50(38% COVID-19 positive). The frequency of postpartum depression was 9(47.4%) among COVID-19 affected mothers and 16(51.6%) in COVID-19 negative mothers. Amongst COVID-19 positive cases, 14(73.7%) were found to be having less than 1 week of COVID-19 positivity, al 19(100%) were practising isolation, 12(63.7%) had less than 7-days of hospitalization,1(5.3%) was admited in Intensive Care Unit, al were satisfied with staff support, 13(68.5%) had a fear of stigma and 1(5.3%) was found to have fear of unemployment. Conclusion: COVID-19 pandemic has had a strong psychological impact on al postpartum mothers irrespective of their infectivity level. This study has highlighted the alarmingly high frequency of postpartum depression in both COVID-19 positive and negative mothers as indicated by high Edinburgh Postpartum Depression Scores (EPDS). [ABSTRACT FROM AUTHOR]

The present study investigates the effect of public–private partnership investment in energy and renewable energy consumption on carbon dioxide (CO2) emissions, taking into account the critical role of economic growth and trade openness in Pakistan from 1992 to 2019. The linear and nonlinear autoregressive distributed lag models are employed to check the co-integration link between dependent and independent variables, further estimate short-run and long-run associations, and examine the symmetric and asymmetric effects of public–private partnership investment in energy on CO2 emissions in Pakistan. The empirical findings show that public–private partnership investment in energy reduces environmental quality by increasing CO2 emissions. Similarly, economic development and trade openness harms the atmosphere by raising CO2 emissions. On the other hand, renewable energy consumption significantly negatively affects CO2 emissions. In addition, the findings also authenticate the asymmetric link between public–private partnership investment in energy and the environment, as CO2 emissions are caused mainly by positive shocks in public–private partnership investment in energy in the short and long run. This study proposes financing renewable energy projects through public–private partnership is needed for an environmentally friendly future. [ABSTRACT FROM AUTHOR]

Quality of Education, especially at Primary level, is an important issue to be discussed at the International Forum. This study highlights the quality of primary education through a comparison of the quality of Community Model Schools and Govt. Girls Primary Schools in Pakistan. Community Model Schools were established under Girls Primary Education Project (GPEP) (Phase I and Phase II), with the cooperation of the Asian Development Bank (ADB). This paper focuses on the study of the effect of facilities, qualifications of headmistresses, dropout rate, results, and co-curricular activities on quality performance of both types of schools. Case study of six Community Model Schools and Govt. Girls Primary Schools were conducted for an in-depth study of the school's performance. The views of the heads of institutions, teachers, students, parents and administrators were collected through semi-structured interview schedules. The researcher personally visited the sample schools one by one to conduct these interviews. All the interviews were video-recorded. Data collected was then analyzed. It was found that Community Model Schools were performing better as compared to Govt. Girls Primary Schools in respect of school results, educational environment, co-curricular activities organized in the schools and overall quality of education.

Human adenoviruses (HAdVs) are a diverse group of viruses associated with respiratory infections in humans worldwide. However, there is a lack of research on the genetic diversity and epidemiology of HAdVs in Pakistan. This study characterized HAdVs in pediatric patients with respiratory tract infections in Karachi, Pakistan, between 2022 and 2023. We analyzed 762 nasopharyngeal samples of children ≤ 5 years. DNA extraction, followed by PCR targeting E2B and hexon genes, was carried out. Data analysis was performed on SPSS 25.0, and phylogenetic analysis of hexon gene was performed on MEGA 11. HAdV was detected in 7.34% (56/762) of patients round the year, but at a significantly higher rate during the winter season. Age was insignificantly associated with HAdV incidence (p = 0.662), but more than 62.5% (35/56) of positive cases were younger than 10 months. The circulating HAdVs were identified as six different types from species B (78.57%) and C (21.42%), with the majority of isolates found to be like B3. HAdV was found to be co-infected with bocavirus (5.4%) and measles (7.14%). These findings revealed a high frequency and genetic diversity of respiratory HAdVs in Karachi, Pakistan. We conclude that periodic and continuous surveillance of adenoviruses and other respiratory pathogens is necessary to improve the prognosis and management of respiratory diseases, thereby reducing the child mortality rate in Pakistan. [ABSTRACT FROM AUTHOR]

Infants are at a higher risk of severe illness with COVID-19 infection compared to older children. While COVID-19 vaccination is not recommended for young infants, they can acquire maternally-derived anti-SARS-CoV-2 antibodies passively through the placenta and breastmilk. We described the persistence of infection-induced maternal antibodies in infant circulation at 9–12 months of age. This was a cross-sectional study nested within the INTERCOVID multinational cohort study. For each COVID positive pregnant woman, two unmatched consecutive COVID negative pregnant women were enrolled between April and September 2020. Women with a positive PCR test, radiographic signs consistent with COVID-19, or at least 2 predefined symptoms of COVID-19 were considered as COVID positive. For this nested cross-sectional study, all COVID positive and either one of the COVID negative participants recruited from the Aga Khan University, Pakistan were approached 9–12 months after delivery, and maternal and infant sera were collected for antibody detection. Altogether, 83 mothers provided consent, of whom 32 (38.6 %) were COVID positive and 51 (61.4 %) were COVID negative during pregnancy. Anti-SARS-CoV-2 antibodies were present in 13 (41 %) infants born to COVID positive and 19 (39 %) infants born to COVID negative mothers (p = 0.87). The presence of reactive antibodies in infants at follow-up was associated with maternal antibodies at follow-up (OR:9.50, 95 % CI:2.03–44.42; p = 0.004). COVID infection occurred in 3 (6 %) infants born to COVID negative mothers while no infant born to a COVID positive mother had a history of infection (p = 0.27). The presence of reactive anti-SARS-CoV-2 antibodies in infants at 9–12 months of age is associated with maternal seropositivity 9–12 months after delivery rather than maternal infection during pregnancy. Further studies are required to validate these findings and assess whether passive immunity in infants is protective against COVID-19 infection. [ABSTRACT FROM AUTHOR]

This article discusses the relationship between leadership dynamics, innovation practices, and green creativity in small and medium-sized enterprises (SMEs) in Pakistan. The study finds that transformational and transactional leadership styles have a positive impact on innovation practices and green creativity. However, green motivation negatively moderates the relationship between innovation practices and green creativity. The study emphasizes the importance of leadership styles and fostering a culture of innovation for driving green creativity in SMEs. Additionally, a list of references is provided for further research on achieving sustainable competitive advantage through green innovation. [Extracted from the article]

Undernutrition is a major public health problem in developing countries. Around 40·2 % of children are stunted in Pakistan. This longitudinal study aimed to assess the effectiveness of locally produced ready-to-use supplementary foods in the prevention of stunting by detecting change in of children in intervention v. control arm against the 2006 WHO growth reference. A community-based non-randomised cluster-controlled trial was conducted from January 2018 to December 2020 in the district of Kurram, Khyber Pakhtunkhwa, Pakistan. A total of 80 clusters (each cluster comprising ≈ 250–300 households) were defined in the catchment population of twelve health facilities. Children aged 6–18 months were recruited n 1680. The intervention included a daily ration of 50 g – locally produced ready-to-use-supplementary food (Wawa-Mum). The main outcome of this study was a change in length for age z-score (LAZ) v. WHO growth standards. Comparison between the interventions was by t test and ANOVA. Cox proportional hazard models were used to assess the association between stunting occurrence and the utilisation of locally produced supplement. Out of the total 1680, fifty-one out of the total 1680, 51·1 out of the total 1680 and 51·1 % (n 859) were male. Mean age 13·9 months (sd + 859) were male. Mean age 13·9 months (sd + –4·4). At baseline, 36·9 % (n 618) were stunted. In the intervention group, mean LAZ score significantly increased from −1·13(2·2 sd) at baseline to −0·93(1·8 sd) at 6-month follow-up (P value 0·01) compared with the control group. The incidence rate of stunting in the intervention arm was 1·3 v. 3·4 per person year in the control arm. The control group had a significantly increased likelihood of stunting (Hazard Ratio (HR) 1·7, 95 % CI 1·46, 2·05, P value < 0·001) v. the intervention group. Locally produced ready-to-use supplementary food is an effective intervention for reducing stunting in children below 2 years of age. This can be provided as part of a malnutrition prevention package to overcome the alarming rates of stunting in Pakistan. [ABSTRACT FROM AUTHOR]

Motorcycle crashes often result in severe injuries on roads that affect people's lives physically, financially, and psychologically. These injuries could be notably harmful to drivers of all age groups. The main objective of this study is to investigate the risk factors contributing to the severity of crash injuries in different age groups. This Objective is achieved by developing accurate machine learning (ML) based prediction models. This research examines the relationship between potential risk factors of motorcycle-associated crashes using (ML) and Shapley Additive explanations (SHAP) technique. The SHAP technique further helped interpreting ML methods for traffic injury severity prediction. It indicates the significant non-linear interactions between dependent and independent variables. The data for this study was collected from the Provincial Emergency Response Service RESCUE 1122 for the Rawalpindi region (Pakistan) over three years (from 2017 to 2020). The Synthetic Minority Oversampling Technique (SMOTE) is employed to balance injury severity classes in the pre-processing phase. The results demonstrate that age, gender, posted speed limit, the number of lanes, and month of the year are positively associated with severe and fatal injuries. This research also assesses how the modeling framework varies between the ML and classical statistical methods. The predictive performance of proposed ML models was assessed using several evaluation metrics, and it is found that Catboost outperformed the XGBoost, Random Forest (RF) and Multinomial Logit (MNL) model. The findings of this study will assist road users, road safety authorities, stakeholders, policymakers, and decision-makers in obtaining substantial and essential guidance for reducing the severity of crash injuries in Pakistan and other countries with prevailing conditions. [ABSTRACT FROM AUTHOR]

Background and Aims: Antibiotic resistance (ABR) is a global public health emergency which has seen an uptick in low‐ to middle‐income countries in recent times due to a plethora of aggravating factors and has led to a whole host of setting‐specific pathogens registering high rates of resistance, causing outbreaks with graver mortality and morbidity. This review analyzes available literature to determine the causes and effects of ABR and recommend solutions to the problem in a Pakistani setting. Methods: Sources for this narrative review were identified via electronic databases using keyword search methods. The information was retrieved using databases such as PubMed and Science Direct. Additionally, websites such as CDC and World Health Organization were used to attain pertinent information. All the sources were selected as per their relevance and appropriateness toward the purpose of this review. Results: This review details the causes by dividing them into three primary strata, namely (1) under‐regulation, (2) over‐prescription and self‐medication, and (3) lack of medical stewardship. This is made much graver when the COVID‐19 pandemic and the subsequent erratic treatment response is considered, with the pandemic augmenting already high levels of consumption. These factors have led a cascade of effects including, but not limited to, a considerable increase in ABR in pathogens to first‐line drugs Conclusion: ABR is a serious and growing issue which will result in undesirable personal, local, and national consequences if unchecked. Mitigation and reversal of this trend is necessary by developing existing programs and investing in novel therapies and pharmaceutical research and strengthening regulatory policies and mechanisms. [ABSTRACT FROM AUTHOR]

Introduction: Schizophrenia is typically treated with medication as the first approach, but additional strategies are necessary to enhance the effectiveness of this treatment for better outcomes. However, it is crucial to explore methods, alongside medication, that promote a positive attitude towards seeking mental health support and alleviate symptom severity among non-institutionalized individuals of different age groups in Pakistan. Thus, this pilot study aimed to utilize a psychoeducation program to enhance patients' motivation and attitudes toward seeking treatment, decrease symptom severity, and investigate the role of financial factors in their illness journey. Methods: In this preliminary investigation, our focus was on individuals who had been diagnosed with schizophrenia and were receiving treatment from various hospitals and primary care clinics. Following a thorough screening process, 255 participants met the eligibility criteria, and 220 completed the psychoeducation program. The study included both male and female participants, with 143 (56.08%) being men and 112 (43.82%) being women. Regarding marital status, 123 (48.24%) were single, 98 (38.43%) were married, and 34 (13.33%) were divorced widowers or widows. The age range of the respondents varied from 18 to 52 years, with a mean age of 35.45 and a standard deviation of 10.27. Results: The results indicated a decrease in symptom severity following a 16-week psychoeducation program. The psychoeducation program significantly reduced the positive symptoms, negative symptoms, and general psychopathological symptoms among patients. Similarly, significant improvement was observed in patients' motivation toward treatment and they actively participated in treatment after getting psychoeducation about the treatment. Similarly, after the psychoeducation program significant improvement was seen in patients' attitudes towards help-seeking and perceived mental health functioning. Conclusion: In summary, the findings suggest that our psychoeducation program has the potential to positively impact the motivation and help-seeking attitudes of schizophrenia patients towards treatment. Moreover, there is a need for further exploration of psychoeducation programs for schizophrenia, particularly in countries facing economic challenges. This study paves the way for the development of an indigenous psychoeducation program tailored to Pakistani schizophrenia patients, with potential applicability for Urduspeaking individuals. [ABSTRACT FROM AUTHOR]

Himalayan regions are among the richest biodiversity reservoirs in the world which contains many eco-regions and protected areas. The present research work was designed to investigate forest resource utilization by local population of Musk Deer National Park (MDNP), Western Himalayas, in the state of Azad Jammu and Kashmir, Pakistan. The data about resource utilizations by local communities were assessed through socio-economic survey in 12 villages. A total of 255 respondents were interviewed. Result revealed that most (44.12%) of the local people relied on forest resources for medicinal uses followed by vegetable collection (17.19%) and fodder extraction (16.10%). In the study area, a total of 109 plant species belonging to 52 families were widely used as medicine, human food (vegetables), fodder (animal food), timber wood and fuel wood by the people of MDNP. The highest number of plant species belonging to family Asteraceae, followed by families Polygonaceae, Poaceae, and Rosaceae. In the present study, 4 different indices were used on the basis of informant consensus for statistical analysis. Each index aimed to determine the cultural significance of a plant species. We emphasized on cultural importance index because it counts the number of uses of a plant species, highly correlated with frequency of citation, and its versatility. Our result showed a significant correlation between numbers of uses (NU), frequency of citation (FC) and semi structural interview that it is still a useful method for compilation study of passive knowledge. The present study revealed that the local knowledge provides new perceptions and opportunities for sustainable and multipurpose use of ecosystem services and offer current approaches for preserving cultural and ecological diversity. From the present study, it is suggested that the protected area should be at the core of national and international effort to conserve nature and their resource that provide benefits us. [ABSTRACT FROM AUTHOR]

Background: The eradication of poliovirus and improving routine immunization (RI) coverage rates present significant challenges in Pakistan. There is a need for interventions that focus on strengthening community engagement to improve routine immunization coverage. Our primary objective is to assess the impact of an integrated strategy designed to enhance community engagement and maternal and child health immunization campaigns on immunization coverage in Pakistan's high-risk union councils of polio-endemic districts. Method: We implemented an integrated approach for routine immunization and maternal and child health in the polio-endemic district of Pakistan. This approach involved setting up health camps and actively engaging and mobilizing the local community. An independent team conducted surveys at three key points: baseline, midline, and endline, to evaluate immunization coverage among children under the age of five. The primary outcome measures for the study were coverage of OPV, IPV, and changes in the proportion of unvaccinated and fully vaccinated children. To select clusters and eligible households in each cluster, we utilized a 30 × 15 cluster sampling technique. Multivariable associations between socio-demographic factors and changes in the proportion of fully vaccinated children at the UC level were assessed using hierarchical linear regression models. Results: A total of 256,946 children under the age of five (122,950 at baseline and 133,996 at endline) were enrolled in the study. By the endline, full immunization coverage had increased to 60% or more in all three study areas compared to the baseline. Additionally, there was a significant increase in the coverage of both OPV and IPV across all three provinces at the endline. The full immunization rates were assessed on three levels of the framework: the distal, intermediate (access and environment), and proximal level (camp attendance and effectiveness). At the distal level, on multivariate analysis, family size was found to be a significant predictor of change in immunity within the families (β = 0.68; p ≤ 0.0001). At the intermediate level, the likelihood of full immunization decreased with the decrease in knowledge about vaccination (β = −0.38; p = 0.002), knowledge about polio vaccine (β = −0.25; p = 0.011), and knowledge about IPV (β = −0.06; p = 0.546). Perceived obstacles to vaccination were fear of adverse events (β = −0.4; p ≤ 0.0001) and lack of education (β = 0.23; p = 0.031), which were found to be significant in bivariate and multivariate analyses. At the proximal level, community mobilization (β = 0.26; p = 0.008) and attendance at health camp (β = 0.21; p ≤ 0.0001) were found to enhance full immunization coverage. On the other hand, the most prominent reason for not attending health camp included no need to attend the health camp as the child was not ill (β = −0.13; p = 0.008). Conclusions: This study found that community mobilization and attendance at health camps significantly enhanced full immunization coverage. The findings highlight the importance of community engagement and targeted interventions in improving immunization coverage and addressing barriers to healthcare seeking. [ABSTRACT FROM AUTHOR]

In Pakistan raw coal and a little quantity of waste plastics are burnt to sustain high temperature inside brick making kilns. The gaseous emissions of the kilns contain a considerable amount of darkish colored particulates. It is currently believed that the plastic burning produces these particulates. Advanced characterization instruments, such as a scanning electron microscope, energy dispersive spectroscopy, X-ray fluorescence, X-ray diffractometer, surface area analyzer using nitrogen gas adsorption isotherms, and thermogravimetric analyzer, were used to find out the chemistry and physics of the particulates. At a magnification of 30,000x, the SEM picture shows masses that are roughly roundish in shape and their size is in between 0.1 to 0.5 microns. The elements detected in these particles are carbon, oxygen, and sulfur (EDS analysis), or in other words, these elements are a typical composition of raw coal. This elemental analysis suggest that fine coal particles come out with usual combustion gases and these emitted particulates are not plastic combustion product. To strengthen this finding, the sample when calcined discarded a significant amount of sulphur oxides species, as determined in the XRF study by noticing a considerable decrease of sulphur content in the calcined particles, suggesting that the particles are actually a coal. The N2 isotherm graph reveals that the light weight flying coal particles has a very low surface area. Additionally, the XRD and TGA studies supports the conclusion that these dark colored particulate emissions are primarily fine coal particles (cenosphere). [ABSTRACT FROM AUTHOR]

Afforestation initiatives are increasingly important in addressing global environmental challenges. This study focuses on the Billion Tree Afforestation Project in Pakistan and investigates the survival and growth of multipurpose species planted under the project in the Dera Ismail Khan Forest Division through a strati$ed random sampling technique. A total of 88 sample plots of 0.1 ha size were taken for 10 plantation sites extended over an area of 461.5 ha. Results showed that the average pit density was 1090 pits/ha, while the regeneration was recorded as 695 individuals/ha. Regarding survival rate, all plantation sites showed the best performance, mostly above 90%. Major species such as Eucalyptus camaldulensis have the highest composition (78%), followed by Acacia nilotica with 14%, while Dalbergia sissoo has the lowest percentage of 7%. The highest growth performance was exhibited by Dalbergia sissoo and Eucalyptus camaldulensis. Dalbergia sissoo attained an average girth of 19 cm and a height of 2.8 m. Eucalyptus camaldulensis attained a mean girth of 16.1 cm and 19.3 cm at 24 months and 27 months, respectively, whereas the mean height at 24 months and 27 months was 2.93 m and 2.8 m, respectively. Furthermore, scatterplots were developed for growth rate analysis between height and girth for all measured individual trees and mean values. The highest correlation was shown by Acacia nilotica with 0.81 and 0.99 coe%cient of determination (R²) for overall individual values and mean values. The study concluded that the plantation activities in the study area were successful as all the sites showed good survival rates. [ABSTRACT FROM AUTHOR]

As part of comprehensive study of lichen diversity of northern areas of Pakistan using molecular and morphological approaches, we found three species of family lecanoraceae belonging to genus Omphalodina and Rhizoplaca. The analyses revealed one new record in the lichen biota of Pakistan namely Rhizoplaca parilis while the other two Omphalodina chrysoleuca and Rhizoplacamelanophthalma are reported for the first time on basis of ITS data, represent new records for Gilgit, Baltistan. Brief descriptions and phylogenetic analyses of the taxa are given. [ABSTRACT FROM AUTHOR]

Background The objective of our study was to measure and give insight into the seropositivity of anti-SARS-CoV-2 antibodies in the patients in our pediatric hospital surgical unit in Pakistan. Methods A prospective cohort study was conducted at a tertiary care pediatric hospital surgical unit in Pakistan between 1 January 2021 and 1 June 2021 on the enrolled neonates and children aged 1 day to 13 years. All patients from three different pediatric strata [neonates (<1 month), infants (1 to 12 months) and children (>1 year)] were enrolled in the study. Results Six-hundred patients were enrolled, and 426 patients were included in the study. Among 426 patients, 234 (54.9%) were male, and 192 (45.1%) were female. Overall only 118 (27.7%) patients developed symptoms. The other 308 (72.3%) were asymptomatic of which 28 (9.1%) had fever, 28 (9.1%) had cough, 38 (12.33%) had body aches, 292 (94.8%) had vomiting/diarrhea, and only 28 (9.1%) developed loss of smell and taste. Our results showed seropositivity of 27.7% (n  = 118), while 72.3% (n  = 308) had negative antibody titers. Conclusion A much higher pediatric SARS-CoV-2 burden of 27.7% was found in our pediatric surgical unit than has previously been reported in the literature of 6.8% for children in pediatric hospitals or pediatric surgical units. Contrary to reporting early in the COVID-19 pandemic, this study determined that children experience a significant burden of COVID-19 infection. Thus, children appear very important in SARS-CoV-2 pandemic, from harboring the virus and further studies need to be done to find if they are transmitting the disease silently. [ABSTRACT FROM AUTHOR]

Microplastic pollution has become a global concern, with potential negative impacts on various ecosystems and wildlife species. Among these species, ducks (Anas platyrhynchos) are particularly vulnerable due to their feeding habits and proximity to aquatic environments contaminated with microplastics. The current study was designed to monitor microplastic (MP) pollutants in the freshwater ecosystem of the Panjkora River, Lower Dir, Pakistan. A total of twenty (20) duck samples were brought up for four months and 13 days on the banks of the river, with no food intake outside the river. When they reached an average weight of 2.41 ± 0.53 kg, all samples were sacrificed, dissected, and transported in an ice box to the laboratory for further analysis. After sample preparation, such as digestion with 10% potassium hydroxide (KOH), density separation, filtration, and identification, the MP content was counted. A total of 2033 MP particles were recovered from 20 ducks with a mean value of 44.6 ± 15.8 MPs/crop and 57.05 ± 18.7 MPs/gizzard. MPs detected in surface water were 31.2 ± 15.5 MPs/L. The major shape types of MPs recovered were fragments in crop (67%) and gizzard (58%) samples and fibers in surface water (56%). Other types of particles recovered were fibers, sheets, and foams. The majority of these detected MP particles were in the size range of 300–500 µm (63%) in crops, and 50–150 µm (55%) in gizzards, while in water samples the most detected particles were in the range of 150–300 µm (61%). Chemical characterization by FTIR found six types of polymers. Low-density polyethylene (LDPE) had the greatest polymer detection rate (39.2%), followed by polyvinyl chloride (PVC) (28.3%), high-density polyethylene (HDPE) (22.7%), polystyrene (6.6%), co-polymerized polypropylene (2.5%), and polypropylene homopolymer (0.7%). This study investigated the presence of microplastics in the crops and gizzards of ducks, as well as in river surface water. The results revealed the significant and pervasive occurrence of microplastics in both the avian digestive systems and the surrounding water environment. These findings highlight the potential threat of microplastic pollution to wildlife and ecosystems, emphasizing the need for further research and effective mitigation strategies to address this pressing environmental concern. [ABSTRACT FROM AUTHOR]

The rise of antimicrobial resistance (AMR) in bacterial pathogens such as Klebsiella pneumoniae (Kp) is a pressing public health and economic concern. The 'One-Health' framework recognizes that effective management of AMR requires surveillance in agricultural as well as clinical settings, particularly in low-resource regions such as Pakistan. Here, we use whole-genome sequencing to characterise 49 isolates of Klebisella spp. (including 43 Kp) and 2 presumptive Providencia rettgeri isolates recovered from dairy farms located near 3 cities in Pakistan—Quetta (n = 29), Faisalabad (n = 19), and Sargodha (n = 3). The 43 Kp isolates corresponded to 38 sequence types (STs), and 35 of these STs were only observed once. This high diversity indicates frequent admixture and limited clonal spread on local scales. Of the 49 Klebsiella spp. isolates, 41 (84%) did not contain any clinically relevant antimicrobial resistance genes (ARGs), and we did not detect any ARGs predicted to encode resistance to carbapenems or colistin. However, four Kp lineages contained multiple ARGs: ST11 (n = 2), ST1391-1LV (n = 1), ST995 (n = 1) and ST985 (n = 1). STs 11, 1391-1LV and 995 shared a core set of five ARGs, including blaCTX-M-15, harboured on different AMR plasmids. ST985 carried a different set of 16 resistance genes, including blaCTX-M-55. The two presumptive P. rettgeri isolates also contained multiple ARGs. Finally, the four most common plasmids which did not harbour ARGs in our dataset were non-randomly distributed between regions, suggesting that local expansion of the plasmids occurs independently of the host bacterial lineage. Evidence regarding how dairy farms contribute to the emergence and spread of AMR in Pakistan is valuable for public authorities and organizations responsible for health, agriculture and the environment, as well as for industrial development. [ABSTRACT FROM AUTHOR]

The World Health Organization (WHO) declared the monkeypox outbreak a public health emergency in June 2022. In Pakistan, positive cases of monkeypox were reported in April 2023. Healthcare workers (HCWs) are considered as a front-line force to combat such outbreaks. A questionnaire-based cross-sectional study was conducted among 11 public sector educational institutions in Punjab, Pakistan, during May and June 2023 among final year medical, pharmacy, and nursing students concerning their knowledge of monkeypox. This included the signs/symptoms of monkeypox. Healthcare students were chosen as they are the HCWs of tomorrow. A total of 389 healthcare students participated in the study, with a mean age of 23.17 ± 1.72 years, and the majority were female. The mean knowledge score was 17.69 ± 4.55 (95% CI 17.24–18.14) out of a maximum total knowledge score of 26 (each correct answer was given a score of 1). The proportion of students with good, moderate, and poor knowledge was 21.6%, 43.2%, and 35.2%, respectively. Age (p = 0.017), gender (p < 0.001), and education (p < 0.001) had a significant impact on the knowledge score. In the multivariate linear regression model, education was the only significant factor linked to knowledge scores. Overall, the majority of future HCWs had moderate knowledge of monkeypox. Consequently, educational activities are needed to improve monkeypox-related knowledge among future HCWs. Furthermore, emerging infectious diseases should be routinely incorporated into HCW curricula. [ABSTRACT FROM AUTHOR]

This exploratory qualitative study problematises how Pakistan's public-school education shapes female identities, employing compulsory school textbooks. Drawing on Foucault's Discourse Analysis and other selected notions, the study also analyses 12 teachers' and 424 students' perspectives on this. The findings highlight Pakistani females' disproportionate and gendered stereotypical social representations in textbooks, which the teachers further reinforce through teaching/social practices in schools. Discursively constructed, most students identify with these and reproduce them when conceptualising an ideal Pakistani woman. The study also underlines how an education system, apparently promising equity and inclusiveness, can be incredibly exclusive, 'guiding' the country's 50% female population to make homemaking their destiny. This education perpetrates social othering, encourages self-righteousness and privileges men over women. Social ramifications of this education entail exclusion and disempowerment of Pakistani women as a social category. This has serious implications for certain sustainable development goals SDGs, 2030, inter alia. [ABSTRACT FROM AUTHOR]

Background. Heavy metal pollution has become a global environmental issue. Heavy metals are contaminating the agro-soils, growing crops, and vegetables through different agricultural practices. In this study, besides the phytoremediation potential of maize, the role of chromium (Cr) and lead (Pb) on crop and soil health has been investigated. Methods. Two maize varieties, Pak-Afgoi and Neelem, were grown under varying concentrations of Cr (50-300 ppm) and Pb (30-300 ppm) and different growth parameters i.e., seed germination, leaf size/number, stem girth, plant height, biomass, chlorophyll content, relative growth rate (RGR), and net assimilation rate (NAR) were studied under Cr and Pb stress. Likewise, the effect of metals was also assessed on different soil characteristics including soil texture, pH, EC, soil organic matter, urease activity and nutrients. Results. Studied plant attributes were adversely affected by heavy metals toxicity. Affected values of RGR and NAR showed a linear correlation with affected growth and dry matter yield of maize. Heavy metals impacted different soil parameters including soil microbial performance and revealed a declining trend as compared to control soil. Maize varieties showed a significant phytoremediation potential i.e., uptake of Cr and Pb was 33% and 22% in Pak-Afgoi, while Neelem showed 38% and 24% at 300 ppm, respectively. Data regarding metal translocation factor (TF), bioaccumulation factor (ACF), and biomagnification ratio (BMR) significantly revealed the potential of maize varieties in the removal of Cr and Pb metals from affected soils. However, Cr-accumulation was higher in shoots, and Pb accumulated in plant roots showed a differential behavior of metal translocation and affinity with the varieties. These maize varieties may be recommended for general cultivation in the Cr and Pb-contaminated areas. [ABSTRACT FROM AUTHOR]

Soil, a significant natural resource, plays a crucial role in supporting various ecosystems and serves as the foundation of Pakistan's economy due to its primary use in agriculture. Hence, timely monitoring of soil type and salinity is essential. However, traditional methods for identifying soil types and detecting salinity are time-consuming, requiring expert intervention and extensive laboratory experiments. The objective of this study is to propose a model that leverages MODIS Terra data to identify soil types and detect soil salinity. To achieve this, 195 soil samples were collected from Lahore, Kot Addu, and Kohat, dating from October 2022 to November 2022. Simultaneously, spectral data of the same regions were obtained to spatially map soil types and salinity of bare land. The spectral reflectance of band values, salinity indices, and vegetation indices were utilized to classify the soil types and predict soil salinity. To perform the classification and regression tasks, the study employed three popular techniques in the research community: Random Forest (RF), Ada Boost (AB), and Gradient Boosting (GB), along with Decision Tree (DT), K-Nearest Neighbor (KNN), and Extra Tree (ET). A 70–30 test train validation split was used for the implementation of these techniques. The efficacy of the multi-class classification models for soil types was evaluated using accuracy, precision, recall, and f1-score. On the other hand, the regression models' performances were evaluated and compared using R-squared (R 2 ), Mean Squared Error (MSE), Mean Absolute Error (MAE), and Root Mean Squared Error (RMSE). The results demonstrated that Random Forest outperformed other methods for both predicting soil types (accuracy = 65.38, precision = 0.60, recall = 0.57, and f1-score = 0.57) and predicting salinity (R 2 = 0.90, MAE = 0.56, MSE = 0.98, RMSE = 0.97). Finally, the study designed a web portal to enable real-time prediction of soil types and salinity using these models. This web portal can be utilized by farmers and decision-makers to make informed decisions regarding soil, crop cultivation, and agricultural planning. [ABSTRACT FROM AUTHOR]

Background: The prevalence of chronic kidney disease is 21.2% in Pakistan and rising due to increasing prevalence of diabetes. Ultrasonography is effective tool in early detection and diagnosis of impaired renal function along with renal function tests. Objective: To study the correlation of serum creatinine with renal echogenicity grading and renal length in patients with chronic kidney disease. Methods: This prospective cross sectional study was conducted at Medicine, Nephrology and Radiology departments of Pakistan Railway General Hospital, Rawalpindi from 30th September 2021 to 15th July 2022. A total of 200 patients with chronic kidney disease were enrolled randomly by consecutive non probability sampling from medical and nephrology in and out patients after fulfilling inclusion and exclusion criteria per study design. After history, physical examination of serum creatinine was done and the patient was sent for ultrasound in the radiology department. Demographic data and study data was entered on pre designed proforma and analyzed using SPSS21. Results: Mean age of the patients was 53.45±8.93 years. There were 110 male and 90 female patients in our study. Mean renal length was 9.55 cm. Mean serum creatinine was 1.921 mg/dl with grade 1 cortical echogenicity, 3.561 with grade 2, 4.267 with grade 3 and 6.001 with grade 4 echogenicity respectively on ultrasound. A negative correlation (r= -0.144) was present between renal length and serum creatinine. Conclusion: Renal cortical echogenicity is a valuable sonographic parameter and is found to be significantly related to serum creatinine level in chronic kidney disease. While renal length showed weaker association with serum creatinine levels. [ABSTRACT FROM AUTHOR]

Enzootic bovine leucosis is caused by bovine leukaemia virus (BLV), a Deltaretrovirus belonging to the family Retroviridae. BLV causes huge economic losses to the dairy industry in the form of decreased milk production, premature culling, and poor reproductive performance of the animals. The aim of the present study was to determine the seroprevalence of BLV infection in buffalo in two districts of Punjab, Pakistan. A total of 384 samples were collected and analysed using a commercial indirect enzyme-linked immunosorbent assay (ELISA) to investigate the seroprevalence of BLV through the detection of the anti-BLV gp51 antibody. A predesigned data questionnaire proforma was employed to find out the association of risk factors with disease. Overall, 18.2% of buffaloes were seropositive for BLV in the study population. The results revealed a significant association (P < 0.05) of age with BLV infection. Furthermore, milk yield and pregnancy had a significant association with the seroprevalence of BLV infection in buffalo whereas no significant association was found with sex, breeding, and health status. Biochemical and oxidative stress markers revealed a significant decrease in liver enzymes alanine transaminase (ALT) and aspartate transaminase (AST), glutathione peroxidase (GPX), and superoxide dismutase (SOD) in seropositive animals as compared to healthy animals. It is concluded that BLV has a considerable prevalence in buffalo in Punjab, Pakistan and there is a dire need to investigate the disease epidemiology at both national and international levels and strategies should be developed to implement an effective control program. [ABSTRACT FROM AUTHOR]

Poliomyelitis is a condition of great concern and is endemic in only two countries of the world: Pakistan and Afghanistan. Community mobilization plays a vital role in raising awareness and can help reduce polio vaccine refusals. The objective of this study will be to decrease polio vaccine refusals and zero-dose vaccines by motivating behavior change through the provision of conditional–collective–community-based incentives (C3Is) based on a reduction in polio vaccine refusals. The project will adopt a pretest/post-test quasi-experimental design with two intervention high-risk union councils (HRUCs) and two control union councils (UCs) of peri-urban (Karachi) and rural (Bannu) settings in Pakistan. A participatory community engagement and demand creation strategy with trust-building community mobilization with C3Is, to reduce vaccine refusals and improve polio immunization coverage in two HRUCs, will be used. These UCs will be divided into clusters based on the polio program framework and community groups will be formed in each cluster. These community groups will carry out awareness activities and will be given serial targets to reduce vaccine refusals and those who qualify will be provided C3Is. The project intends to create a replicable model that the government can integrate within health systems for long-term sustainability until the goal of eradication of poliovirus is achieved. The evaluation will be carried out by an independent data collection and analysis team at baseline and endline (after 12 months of intervention). The trial is registered with linicalTrials.gov with number NCT05721274. [ABSTRACT FROM AUTHOR]

Introduction: The World Health Organization recommends regular monitoring of the efficacy of nationally recommended antimalarial drugs. We present the results of studies on the efficacy of recommended antimalarials and molecular markers of artemisinin and partner resistance in Afghanistan, Pakistan, Somalia, Sudan and Yemen. Methods: Single‐arm prospective studies were conducted to evaluate the efficacy of artesunate‐sulfadoxine‐pyrimethamine (ASSP) in Afghanistan and Pakistan, artemether‐lumefantrine (AL) in all countries, or dihydroartemisinin‐piperaquine (DP) in Sudan for the treatment of Plasmodium falciparum. The efficacy of chloroquine (CQ) and AL for the treatment of Plasmodium vivax was evaluated in Afghanistan and Somalia, respectively. Patients were treated and monitored for 28 (CQ, ASSP and AL) or 42 (DP) days. Polymerase chain reaction (PCR)‐corrected cure rate and parasite positivity rate at Day 3 were estimated. Mutations in the P. falciparum kelch 13 (Pfk13) gene and amplifications of plasmepsin (Pfpm2) and multidrug resistance‐1 (Pfmdr‐1) genes were also studied. Results: A total of 1680 (249 for ASSP, 1079 for AL and 352 for DP) falciparum cases were successfully assessed. A PCR‐adjusted ASSP cure rate of 100% was observed in Afghanistan and Pakistan. For AL, the cure rate was 100% in all but four sites in Sudan, where cure rates ranged from 92.1% to 98.8%. All but one patient were parasite‐free at Day 3. For P. vivax, cure rates were 98.2% for CQ and 100% for AL. None of the samples from Afghanistan, Pakistan and Yemen had a Pfk13 mutation known to be associated with artemisinin resistance. In Sudan, the validated Pfk13 R622I mutation accounted for 53.8% (14/26) of the detected non‐synonymous Pfk13 mutations, most of which were repeatedly detected in Gadaref. A prevalence of 2.7% and 9.3% of Pfmdr1 amplification was observed in Pakistan and Yemen, respectively. Conclusion: High efficacy of ASSP, AL and DP in the treatment of uncomplicated falciparum infection and of CQ and AL in the treatment of P. vivax was observed in the respective countries. The repeated detection of a relatively high rate of Pfk13 R622I mutation in Sudan underscores the need for close monitoring of the efficacy of recommended ACTs, parasite clearance rates and Pfk13 mutations in Sudan and beyond. Registration numbers of the trials: ACTRN12622000944730 and ACTRN12622000873729 for Afghanistan, ACTRN12620000426987 and ACTRN12617001025325 for Pakistan, ACTRN12618001224213 for Somalia, ACTRN12617000276358, ACTRN12622000930785 and ACTRN12618001800213 for Sudan and ACTRN12617000283370 for Yemen. [ABSTRACT FROM AUTHOR]

The main objective of this research is to investigate the approaches used to integrate rural women working with Sindhiyani Tehreek and Aurat Foundation in Pakistan into sustainable development programs and determine the impact of such efforts on individual empowerment, community resilience, and overall socioeconomic development. The research has adopted a qualitative approach to collect the primary and secondary sources of data. The data is analyzed using thematic analysis. The study has also incorporated case studies of the Aurat Foundation and Sindhiyani Tehreek to trace the role of rural women in sustainable development projects in Pakistan. The findings of this study are set to add to academic knowledge as well as policy formulation. Policymakers, NGOs, and development practitioners can plan and implement more targeted and impactful interventions if given strategies are effectively implemented that integrate rural women of these organizations into sustainable development programs. Finally, this research aims to inform a more inclusive and gender-sensitive approach to sustainable development, ensuring that women working in Sindhiyani Tehreek and Aurat Foundation have been playing a key role in sustainable development projects in Pakistan. [ABSTRACT FROM AUTHOR]