601. Proteomic and physiological assessment of stress sensitive and tolerant variety of tomato treated with brassinosteroids and hydrogen peroxide under low-temperature stress.
- Author
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Khan TA, Yusuf M, Ahmad A, Bashir Z, Saeed T, Fariduddin Q, Hayat S, Mock HP, and Wu T
- Subjects
- Antioxidants analysis, Chlorophyll analysis, Solanum lycopersicum chemistry, Solanum lycopersicum drug effects, Photosynthesis drug effects, Proteomics, Stress, Physiological physiology, Brassinosteroids pharmacology, Cold Temperature, Hydrogen Peroxide pharmacology, Solanum lycopersicum growth & development, Plant Proteins analysis, Steroids, Heterocyclic pharmacology, Stress, Physiological drug effects
- Abstract
The aim of current investigation was to perform proteomics and physio-chemical studies to dissect the changes in contrasting varieties (S-22 and PKM-1) of Lycopersicon esculentum under low-temperature stress. Plant grown under variable low-temperature stress were analysed for their growth biomarkers, antioxidant enzyme activities, and other physiological parameters, which headed toward the determination of protein species responding to low-temperature and 24-epibrassinolide (EBL) concentrations. The plants grown under temperatures, 20/14, 12/7, and 10/3 °C recorded significantly lower growth biomarkers, SPAD chlorophyll, net photosynthetic rate and carbonic anhydrase activity in S-22 and PKM-1. Moreover, the combined effect of EBL and hydrogen peroxide (H
2 O2 ) significantly improved the parameters mentioned above and consecutively upgraded the different antioxidant enzymes (CAT and SOD) with higher accumulation of proline under stress and stress-free environments. Furthermore, proteomics study revealed that the maximum number of differentially expressed proteins were detected in S-22 (EBL + H2 O2 ); while treatment with EBL + H2 O2 + low temperature lost expression of 20 proteins. Overall, three proteins (O80577, Q9FJQ8, and Q9SKL2) took a substantial part in the biosynthesis of citrate cycle pathway and enhanced the growth and photosynthetic efficiency of tomato plants under low-temperature stress., (Copyright © 2019 Elsevier Ltd. All rights reserved.)- Published
- 2019
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