51. Altered protein kinase activities of lymphoid cells transformed by Abelson and Moloney leukemia viruses
- Author
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Lorenzo A. Pinna, Anna Maria Brunati, Daniela Saggioro, and Luigi Chieco-Bianci
- Subjects
Abelson murine leukemia virus ,Lymphoma ,viruses ,T-Lymphocytes ,Biophysics ,Mitogen-activated protein kinase kinase ,Abelson leukemia virus ,Biochemistry ,Cell Line ,Mice ,Structural Biology ,hemic and lymphatic diseases ,Genetics ,Cyclic AMP ,Animals ,(Thymocyte) ,c-Raf ,Moloney leukemia virus ,Molecular Biology ,Protein Kinase C ,Mice, Inbred BALB C ,biology ,Chemistry ,Cyclin-dependent kinase 2 ,Casein kinase 2 Tyrosine protein kinase ,Cell Biology ,biochemical phenomena, metabolism, and nutrition ,Protein-Tyrosine Kinases ,biology.organism_classification ,Cell Transformation, Viral ,Protein kinase R ,Virology ,Molecular biology ,Leukemia Virus, Murine ,Cell Transformation, Neoplastic ,biology.protein ,Cyclin-dependent kinase 9 ,Casein kinase 2 ,Moloney murine leukemia virus ,Casein Kinases ,Protein Kinases - Abstract
Five different types of protein kinase acivities have been evaluated in cell lines from murine lymphomas induced by Abelson leukemia virus (A-MuLV), whose oncogene codes for a tyrosine protein kinase. Such activities were compared with those of normal cells and of cells transformed by Moloney leukamia virus (M-MuLV), lacking oncogene sequences in its genome. While cAMP-dependent protein kinase and casein kinase-1 do not undergo significant changes, casein kinase-2 rises in both A-MuLV and M-MuLV infected lymphocytes, becoming largely associated with the particulate fraction of transformed cells. Protein kinase-C on the other hand is unchanged in M-MuLV transformed cells but it undergoes a 2–3-fold increment in both soluble and particulate fractions of A-MuLV transformed lymphocytes, which also display high tyrosine protein kinase activity.
- Published
- 1986